1.
Biosens Bioelectron
; 10(8): 693-704, 1995.
Article
in English
| MEDLINE
| ID: mdl-7576437
ABSTRACT
Amperometric glucose sensors were constructed based on solid graphite electrodes, surface-modified with NAD+ dependent glucose dehydrogenase (GDH), Toluidine Blue O (TBO), and protective ionic polymers. The electrocatalytic oxidation of NADH was evaluated from cyclic voltammetry with TBO dissolved, adsorbed, and electrostatically or covalently bound to polymers. The NADH and glucose sensors constructed were investigated and operated at 0 mV vs. Ag/AgCl using single potential step chronoamperometry. The operational stability of the glucose sensors was limited by leakage of NAD+. A glucose sensitivity much higher than carbon paste electrode was found. A sensitivity as high as 25 microA cm-2 mM-1 was achieved.
Subject(s)
Biosensing Techniques , Glucose Dehydrogenases , Glucose/analysis , NAD/analysis , Tolonium Chloride , Enzymes, Immobilized , Glucose 1-Dehydrogenase , Oxidation-Reduction
2.
J Theor Biol
; 65(2): 379-91, 1977 Mar 21.
Article
in English
| MEDLINE
| ID: mdl-853756
Subject(s)
Enzymes , Membranes, Artificial , Electric Conductivity , Electron Transport , Energy Transfer , Kinetics , Oils , Potentiometry , Water
3.
FEBS Lett
; 65(2): 155-8, 1976 Jun 01.
Article
in English
| MEDLINE
| ID: mdl-1278416
4.
Nature
; 259(5543): 494-6, 1976 Feb 12.
Article
in English
| MEDLINE
| ID: mdl-130557
5.
FEBS Lett
; 50(2): 223-6, 1975 Feb 01.
Article
in English
| MEDLINE
| ID: mdl-163209
Subject(s)
Adenosine Triphosphatases/metabolism , Bacterial Proteins/metabolism , Cytochrome Reductases/metabolism , Electron Transport Complex IV/metabolism , Mitochondria, Muscle/enzymology , Retinal Pigments/metabolism , Rhodopsin/metabolism , Alkanes , Animals , Cattle , Cytochrome c Group , Electron Transport , Kinetics , Myocardium , Oxidative Phosphorylation Coupling Factors/metabolism , Potentiometry , Water
6.
Biochim Biophys Acta
; 368(1): 22-8, 1974 Oct 18.
Article
in English
| MEDLINE
| ID: mdl-4371323