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1.
Physiol Genomics ; 5(3): 137-45, 2001 Apr 02.
Article in English | MEDLINE | ID: mdl-11285367

ABSTRACT

The cDNA of an uncoupling protein (UCP) homolog has been cloned from the swallow-tailed hummingbird, Eupetomena macroura. The hummingbird uncoupling protein (HmUCP) cDNA was amplified from pectoral muscle (flight muscle) using RT-PCR and primers for conserved domains of various known UCP homologs. The rapid amplification of cDNA ends (RACE) method was used to complete the cloning of the 5' and 3' ends of the open reading frame. The HmUCP coding region contains 915 nucleotides, and the deduced protein sequence consists of 304 amino acids, being approximately 72, 70, and 55% identical to human UCP3, UCP2, and UCP1, respectively. The uncoupling activity of this novel protein was characterized in yeast. In this expression system, the 12CA5-tagged HmUCP fusion protein was detected by Western blot in the enriched mitochondrial fraction. Similarly to rat UCP1, HmUCP decreased the mitochondrial membrane potential as measured in whole yeast by uptake of the fluorescent potential-sensitive dye 3',3-dihexyloxacarbocyanine iodide. The HmUCP mRNA is primarily expressed in skeletal muscle, but high levels can also be detected in heart and liver, as assessed by Northern blot analysis. Lowering the room's temperature to 12-14 degrees C triggered the cycle torpor/rewarming, typical of hummingbirds. Both in the pectoral muscle and heart, HmUCP mRNA levels were 1.5- to 3.4-fold higher during torpor. In conclusion, this is the first report of an UCP homolog in birds. The data indicate that HmUCP has the potential to function as an UCP and could play a thermogenic role during rewarming.


Subject(s)
Birds/genetics , Carrier Proteins/genetics , Membrane Proteins/genetics , Membrane Transport Proteins , Mitochondrial Proteins , Proteins/genetics , Amino Acid Sequence , Animals , Birds/physiology , Cloning, Molecular , Ion Channels , Membrane Potentials , Mitochondria/physiology , Molecular Sequence Data , Phylogeny , RNA, Messenger/biosynthesis , Saccharomyces cerevisiae/physiology , Sequence Homology, Amino Acid , Thermogenesis , Tissue Distribution , Uncoupling Protein 1 , Uncoupling Protein 2 , Uncoupling Protein 3
2.
Int J Obes Relat Metab Disord ; 24(8): 1065-8, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10951548

ABSTRACT

OBJECTIVE: To investigate whether the region of chromosome 11 (11q13) containing the genes UCP2 and UCP3 could be excluded for linkage with a variety of obesity-related phenotypes in humans. DESIGN: Exclusion mapping using a variance component approach in extended pedigrees. SUBJECTS: Four-hundred and fifty eight individuals (195 females, 263 males) distributed in 10 Mexican American families of probands randomly ascertained with respect to any disease state and who are participating in the San Antonio Family Heart Study. Ages range from 18 to 87 (mean age 35 y). MEASUREMENTS: Serum leptin levels, fat mass (FM), body mass index (BMI), and waist circumference. RESULTS: We were able to exclude the chromosomal region containing UCP2/UCP3 as having an effect on this set of obesity-related phenotypes at relative effect sizes of 10% or greater (P-values<0.05). CONCLUSIONS: These results suggest that variation in these genes is unlikely to have a substantial effect on the expression of obesity-related phenotypes in the Mexican American population.


Subject(s)
Carrier Proteins/genetics , Chromosomes, Human, Pair 11 , Membrane Transport Proteins , Mexican Americans/genetics , Mitochondrial Proteins , Obesity/genetics , Proteins/genetics , White People/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Anthropometry , Chromosome Mapping , Female , Humans , Ion Channels , Leptin/blood , Male , Middle Aged , Pedigree , Phenotype , Texas , Uncoupling Protein 2 , Uncoupling Protein 3
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