ABSTRACT
A 41-yr-old patient experienced an anaphylactic shock reaction caused by formaldehyde in a root canal sealant during endodontic treatment. The clinical events, positive skin tests, and a high level of immunoglobin E to formalin RAST (class 4) suggest the involvement of immunoglobin E-dependent mechanisms toward formaldehyde. This very infrequent observation in endodontic therapy focuses attention on the different pathological manifestations related to formalin, their mechanisms, and the prevention possibilities in dentistry.
Subject(s)
Anaphylaxis/chemically induced , Drug Hypersensitivity/etiology , Formaldehyde/adverse effects , Root Canal Filling Materials/adverse effects , Adult , Extravasation of Diagnostic and Therapeutic Materials/complications , Humans , Immunoglobulin E/immunology , Male , Polymers/adverse effects , Resorcinols/adverse effects , Root Canal Filling Materials/chemistry , Skin TestsABSTRACT
The case of GAPO syndrome reported here is the 24th recorded case, 23 cases having been published previously. The 29-year-old male under discussion presents all the typical features of the syndrome, having short stature, dysmorphic craniofacial features, total alopecia and pseudoanodontia. Orally, the erupted primary dentition was extremely worn and on radiographic examination, the second mandibular molars were found to be unerupted, together with the entire permanent dentition. Cephalometry revealed the absence of facial pneumatisation, a deficient cranial base with diminished upper face height and maxillary and mandibular hypoplasia with a prognathic skeletal pattern. Histological examination of an extracted primary incisor and its surrounding root bone revealed extensive ankylosis. This paper describes in detail the clinical findings and reviews, and discusses previously published cases in relation to the present one. As with prior cases, parental consanguinity was present in the pedigree.
Subject(s)
Abnormalities, Multiple/diagnosis , Alopecia/diagnosis , Anodontia/diagnosis , Eye Abnormalities/diagnosis , Growth Disorders/diagnosis , Optic Atrophy/diagnosis , Adult , Anodontia/pathology , Eye Abnormalities/pathology , Humans , Male , Optic Atrophy/pathology , Phenotype , SyndromeSubject(s)
Follicular Cyst/pathology , Adolescent , Child , Follicular Cyst/surgery , Humans , Molar, Third/pathologyABSTRACT
The effects of transferrin on the proliferation kinetics of these cells from day-14 lower first molars, cultured for 2-6 days in a chemically defined medium supplemented with 5 and 50 micrograms/ml of human diferric transferrin, were studied. Transferrin stimulated the mitotic and [3H]-thymidine labelling indices. These data were correlated with immunolocalization of the transferrin receptor using indirect immunofluorescence and specific monoclonal antibodies. The presence of specific transferrin receptors in pre-odontoblasts and pre-ameloblasts, and in ameloblasts of older teeth (day-18 to day-21), was also assessed by indirect immunofluorescence and binding experiments using iodinated transferrin.
Subject(s)
Odontogenesis/drug effects , Tooth/embryology , Transferrin/pharmacology , Ameloblasts/drug effects , Animals , Cell Division/drug effects , Mice , Mice, Inbred Strains , Molar , Odontoblasts/drug effects , Organ Culture Techniques , Stimulation, ChemicalSubject(s)
Collagen/therapeutic use , Freeze Drying , Hemostasis, Surgical/methods , Tooth Extraction , Cellulose, Oxidized , Child , Child, Preschool , Female , Humans , Male , Postoperative Care , Tooth, DeciduousABSTRACT
The accumulation of sulfated GAG in embryonic mouse molars before, during, and after terminal differentiation of odontoblasts was localized by [35S]autoradiography combined with the use of chondroitin ABC lyase. Much more sulfated GAG were accumulated in the dental papilla than in the dental epithelium. High incorporation of [35S]sulfate occurred at the epithelio-mesenchymal junction, which is the site of dental basement membrane and predentin. Before terminal differentiation of odontoblasts, the distribution of sulfated GAG was uniform at the basement membrane. After the onset of terminal differentiation of odontoblasts, much more sulfated GAG accumulated at the tip of principal cusps than at the apical (inferior) parts of cusps, and sulfated GAG were then found to be degraded more rapidly at the epithelio-mesenchymal junction than at other parts of the tooth germ. Thus regional variation in the rate of degradation of GAG exists in the tooth germs. Trypsin-isolated dental epithelia cultured in vitro synthesized a new basement membrane that could be labeled with [3H]glucosamine but not with 35SO4(-2). The epithelial-derived basal lamina contains little or no sulfatated GAG.
Subject(s)
Glycosaminoglycans/metabolism , Sulfur/metabolism , Tooth Germ/metabolism , Animals , Autoradiography , Mice , Mice, Inbred Strains , Molar , Odontoblasts/metabolism , Sulfur Radioisotopes , Tooth Germ/cytologyABSTRACT
First mandibular embryonic mouse molars (day 18) were cultured for 8 days. Maintenance of crown pattern and amelogenesis were studied in function of different culture conditions. Grown on top of semi-solid coagulum (composed of cock plasma, embryonic extract, fetal calf serum and MEM or BGjb) the typical crown pattern was always conserved. Amelogenesis existed in 85% if the coagulum was composed of 60% BGjb, 30% plasma, 10% embryonic extract and 180 microgram/ml ascorbic acid. Embryonic extract did not inhibit the mineralization. Grown on Millipore filter in the presence of different media (MEM or BGJb supplemented with serum, embryonic extract and ascorbic acid) the crown pattern was always disturbed. Nevertheless amelogenesis was generally initiated. In absence of serum and embryonic extract, chemically defined media (MEM or BGjb, supplemented with glutamine, glycine and ascorbic acid) allowed functional differentiation of odontoblasts and polarization of ameloblasts. These cells did not secrete enamel. If these defined media were devoid of ascorbic acid, predentin was secreted but necrosis of dental papilla cells was observed.