ABSTRACT
Measurement of oxygen tension in compressed collagen sheets was performed using matrix-embedded optical oxygen sensors based on platinum(II) and palladium(II) porphyrins supported on polyacrylamide nanoparticles. Bespoke, fully water-soluble, mono-functionalised Pt(II) and Pd(II) porphyrin complexes designed for conjugation under mild conditions were obtained using microwave-assisted metallation. The new sensors display a linear response (1/τ vs. O2) to varying oxygen tension over a biologically relevant range (7.0 × 10(-4) to 2.7 × 10(-1) mM) in aqueous solutions; a behaviour that is maintained following conjugation to polyacrylamide nanoparticles, and following embedding of the nanosensors in compressed collagen sheets, paving the way to innovative approaches for real-time resolution of oxygen gradients throughout 3D matrices useful for tissue regeneration.
Subject(s)
Coordination Complexes/chemistry , Molecular Probes/chemistry , Oxygen/analysis , Palladium/chemistry , Platinum/chemistry , Porphyrins/chemistry , Spectrometry, Fluorescence , Acrylic Resins/chemistry , Collagen/chemistry , Coordination Complexes/chemical synthesis , Microwaves , Nanoparticles/chemistry , Tissue Engineering , Water/chemistryABSTRACT
Polyacrylamide nanoparticles bearing two orthogonal reactive functionalities were prepared by reverse microemulsion polymerisation. Water-soluble photosensitisers and peptide or carbohydrate moieties were sequentially attached to the new nanospecies by orthogonal conjugations based on copper-catalysed azide-alkyne cycloaddition and isothiocyanate chemistry.
Subject(s)
Acrylic Resins/chemical synthesis , Crystallization/methods , Nanostructures/chemistry , Nanostructures/ultrastructure , Nanotechnology/instrumentation , Nanotechnology/methods , Macromolecular Substances/chemistry , Materials Testing , Molecular Conformation , Particle Size , Surface PropertiesABSTRACT
BACKGROUND: The possibility of eradicating cancer by selective destruction of tumour blood vessels may represent an attractive therapeutic avenue, but most pharmaceutical agents investigated so far did not achieve complete cures and are not completely specific. Antibody conjugates now allow us to evaluate the impact of selective vascular shutdown on tumour viability and to study mechanisms of action. METHODS: We synthesised a novel porphyrin-based photosensitiser suitable for conjugation to antibodies and assessed anticancer properties of its conjugate with L19, a clinical-stage human monoclonal antibody specific to the alternatively spliced EDB domain of fibronectin, a marker of tumour angiogenesis. RESULTS: Here we show in two mouse model of cancer (F9 and A431) that L19 is capable of highly selective in vivo localisation around tumour blood vessels and that its conjugate with a photosensitiser allows selective disruption of tumour vasculature upon irradiation, leading to complete and long-lasting cancer eradication. Furthermore, depletion experiments revealed that natural killer cells are essential for the induction of long-lasting complete responses. CONCLUSIONS: These results reinforce the concept that vascular shutdown can induce a curative avalanche of tumour cell death. Immuno-photodynamic therapy may be particularly indicated for squamous cell carcinoma of the skin, which we show to be strongly positive for markers of angiogenesis.
Subject(s)
Immunoconjugates/administration & dosage , Neoplasms, Experimental/blood supply , Neoplasms, Experimental/drug therapy , Neovascularization, Pathologic/drug therapy , Photochemotherapy , Animals , Carcinoma, Squamous Cell/drug therapy , Fluorescent Antibody Technique , Humans , Killer Cells, Natural/physiology , Mice , Mice, Inbred BALB CABSTRACT
Photodynamic sensitizers are drugs activated by light of a specific wavelength and are used in the photodynamic therapy (PDT) of certain diseases. Second- and third-generation photosensitizers with improved PDT properties are now under investigation. In this issue of the British Journal of Pharmacology, Leung et al. have described the synthesis and investigation of a second-generation photosensitizer (BAM-SiPc) targeted towards the cells of HepG2 and HT29 tumours. BAM-SiPc is selectively functionalized with bis-amino groups and has demonstrated potent PDT activity in a small animal model. However, it also exhibited non-selective distribution and accumulation in multiple animal (small mouse) organs and tissue. These issues highlight the importance and need for good biodistribution and localization properties for an efficacious photosensitizer. The lack of tumour specificity may have a significant impact on the potential BAM-SiPc has in clinical PDT.
Subject(s)
Photochemotherapy , Photosensitizing Agents/therapeutic use , Animals , Humans , Neoplasms/therapy , Photosensitizing Agents/adverse effects , Photosensitizing Agents/pharmacologyABSTRACT
A promising approach to increase the specificity of photosensitizers used in photodynamic therapy has been through conjugation to monoclonal antibodies (MAb) directed against tumour-associated antigens. Many of the conjugations performed to date have relied on the activated ester method, which can lead to impure conjugate preparations and antibody crosslinking. Here, we report the development of photosensitizer-MAb conjugates utilising two porphyrin isothiocyanates. The presence of a single reactive isothiocyanate allowed facile conjugation to MAb FSP 77 and 17.1A directed against internalizing antigens, and MAb 35A7 that binds to a non-internalizing antigen. The photosensitizer-MAb conjugates substituted with 1-3 mol of photosensitizer were characterised in vitro. No appreciable loss of immunoreactivity was observed and binding specificity was comparable to that of the unconjugated MAb. Substitution with photosensitizer had a minimal effect on antibody biodistribution in vivo for the majority of the conjugates, although a decreased serum half-life was observed using a cationic photosensitizer at the higher loading ratios. Tumour-to-normal tissue ratios as high as 33.5 were observed using MAb 35A7 conjugates. The internalizing conjugate showed a higher level of phototoxicity as compared with the non-internalizing reagent, using a cell line engineered to express both target antigens. These data demonstrate the applicability of the isothiocyanate group for the development of high-quality conjugates, and the use of internalizing MAb to significantly increase the photodynamic efficiency of conjugates during photoimmunotherapy.
Subject(s)
Antibodies, Monoclonal/pharmacokinetics , Immunotherapy/methods , Immunotoxins/pharmacokinetics , Isothiocyanates/chemistry , Photochemotherapy/methods , Porphyrins/chemistry , Animals , Antibody Specificity , Cell Line, Tumor , Flow Cytometry , Humans , Isothiocyanates/immunology , Male , Mice , Mice, Nude , Photosensitizing Agents/chemistry , Photosensitizing Agents/immunology , Porphyrins/immunology , Tissue DistributionABSTRACT
A novel method for conjugating porphyrins and related molecules to proteins has been developed. The method, which involves synthesizing porphyrins, chlorins, and bacteriochlorins bearing a single amine-reactive isothiocyanate group represents a facile system for protein labeling with these photoactive species. Problems associated with the noncovalent binding of porphyrins to proteins are highlighted, and a method for purifying conjugates to yield exclusively covalently bound porphyrin protein species is demonstrated. Biological activity of porphyrin-bovine serum albumin conjugates formed and purified by these methods is demonstrated using laser scanning confocal microscopy.
Subject(s)
Isothiocyanates/chemistry , Photosensitizing Agents/chemical synthesis , Porphyrins/chemistry , Serum Albumin, Bovine/chemistry , Animals , Cattle , Cells, Cultured , Drug Carriers/chemical synthesis , Drug Carriers/chemistry , Electrophoresis, Polyacrylamide Gel , HeLa Cells , Humans , Isothiocyanates/administration & dosage , Microscopy, Confocal , Molecular Conformation , Molecular Probes/chemical synthesis , Molecular Probes/chemistry , Molecular Structure , Photochemistry , Photochemotherapy , Photosensitizing Agents/chemistry , Porphyrins/administration & dosage , Protein Binding , Serum Albumin, Bovine/administration & dosageABSTRACT
The first synthesis of two porphyrin-phthalocyanine heterodimers with a direct triple bond linkage between the macrocycles, designed for the study of photo-induced energy and electron transfer processes, is presented along with preliminary ground state and fluorescence spectroscopic data.
ABSTRACT
A series of straight chain, branched and cyclo-delta-aminolaevulinic acid (ALA) esters have been synthesized and their photosensitizing properties analysed using an in vitro system of rat pancreatoma cells. Structurally favourable ALA esters not only induced the formation of more of the endogenous photosensitizer, protoporphyrin IX (PpIX), but they did so at a faster rate than ALA itself. This action was reflected in a substantial increase in photocytotoxicity of some 270 times, using the more potent ALA esters. An important structural feature was identified in two of the ALA esters which greatly limited PpIX production, i.e. a branch point located next to the site of ester cleavage. Experiments on the transport of ALA and of ALA esters across the cell membrane showed that ALA, but not ALA esters, gain access to the cell via the di- and tripeptide transporter, PEPTI. Finally, these results show that the esterification of ALA can greatly increase its cellular uptake, so generating more intracellular PpIX, improved tumour cell photosensitization and enhanced photocytotoxicity.
Subject(s)
Aminolevulinic Acid/analogs & derivatives , Pancreatic Neoplasms/drug therapy , Photochemotherapy/methods , Photosensitizing Agents/pharmacology , Symporters , Aminolevulinic Acid/chemical synthesis , Aminolevulinic Acid/pharmacology , Animals , Biological Transport, Active , Carrier Proteins/metabolism , Esters/chemical synthesis , Esters/pharmacokinetics , Esters/pharmacology , Pancreatic Neoplasms/metabolism , Peptide Transporter 1 , Photosensitizing Agents/chemical synthesis , Photosensitizing Agents/pharmacokinetics , Protoporphyrins/pharmacokinetics , Protoporphyrins/pharmacology , Rats , Spectrometry, Fluorescence , Structure-Activity Relationship , Tumor Cells, CulturedABSTRACT
mAb B4 is a monoclonal antibody directed against HIV receptor complex. The antibody had broad neutralizing activity against HIV and provided postexposure prophylaxis to hu-peripheral blood leukocyte (PBL)-severe combined immunodeficient mice and chimpanzees. B4 recognized a complex receptor site for HIV on the T cell surface that includes CD4 and also may be influenced by interaction with HIV coreceptors. mAb B4 preferentially neutralized primary HIV-1 isolates compared with T cell line-adapted strains, including syncytium-inducing and non-syncytium-inducing phenotypes, representatives from HIV-1 subtypes A-G, as well as HIV-2, simian immunodeficiency virus, and chimeric simian/human immunodeficiency virus (SHIV). Neutralization was demonstrated in both pre- and postinfection models. The administration of mAb B4 after infectious challenge totally interrupted the infection of hu-PBL-severe combined immunodeficient mice by PBL-grown HIV-1 and the infection of chimpanzees by chimp-adapted HIV-1. This mode of protection suggested that the anti-HIV receptor antibody is efficacious for prophylaxis after exposure to HIV and for prevention of maternal transmission and may be an effective antiretroviral agent for treatment.
Subject(s)
Antibodies, Monoclonal/pharmacology , CD4 Antigens/immunology , HIV-1/immunology , Immunotherapy , Leukocytes/immunology , Receptors, HIV/immunology , Animals , Antibodies, Monoclonal/therapeutic use , HIV-1/classification , HIV-1/isolation & purification , Homosexuality, Male , Humans , Male , Mice , Mice, Inbred BALB C , Mice, SCID , Neutralization Tests , Pan troglodytes , Viral Plaque AssayABSTRACT
A synthetic-peptide-based enzyme-linked immunosorbent assay (EIA) capable of screening for antibodies to both human immunodeficiency virus type 1 (HIV-1) and HIV-2 has been developed for use in blood banks and diagnostic laboratories. Microtiter wells are coated with two synthetic peptides, one corresponding to the highly conserved envelope region of HIV-1 and another corresponding to the conserved envelope region of HIV-2. Overall, sensitivity was 100% in 303 individuals diagnosed with AIDS and 96 individuals diagnosed with AIDS-related complex, 14.8% in a study of 500 high-risk group members, 99.9% in 600 EIA repeatedly reactive (RR)-HIV-1 Western blot (WB)-positive repository specimens, and 100% for 222 geographically diverse HIV-1 specimens and 216 confirmed HIV-2-positive specimens evaluated. The specificity was determined to be 99.72% for a total of 13,004 serum and plasma samples from random volunteer donors evaluated across five blood banks. Forty donors who were found to be EIA RR-WB indeterminate but nonreactive on the United Biomedical, Inc., test (UBI HIV 1/2 EIA) were prospectively followed as an additional measure of specificity. None of the 40 low-risk cases evolved into a positive WB pattern at follow-up. The sensitivity and specificity of this new assay are comparable to those of other Food and Drug Administration-licensed HIV-1 and HIV-1-HIV-2 assays that are currently available in the United States. The UBI HIV 1/2 EIA affords laboratories another choice in the detection of antibodies for HIV-1 and HIV-2 with a test based on an alternative antigen format.
Subject(s)
AIDS Serodiagnosis , HIV Antibodies/blood , HIV-1/immunology , HIV-2/immunology , Blotting, Western , Enzyme-Linked Immunosorbent Assay , Evaluation Studies as Topic , Humans , Sensitivity and Specificity , Vaccines, SyntheticABSTRACT
Photodynamic therapy (PDT) has, during the last quarter century, developed into a fully fledged biomedical field with its own association, the International Photodynamic Association (IPA) and regular conferences devoted solely to this topic. Recent approval of the first PDT sensitizer, Photofrin (porfimer sodium), by health boards in Canada, Japan, the Netherlands and United States for use against certain types of solid tumors represents, perhaps, the single most significant-indicator of the progress of PDT from a laboratory research concept to clinical reality. The approval of Photofrin will undoubtedly encourage the accelerated development of second-generation photosensitizers, which have recently been the subject of intense study. Many of these second-generation drugs show significant differences, when compared to Photofrin, in terms of treatment times postinjection, light doses and drug doses required for optimal results. These differences can ultimately be attributed to variations in either the quantum efficiency of the photosensitizer in situ, which is in turn affected by aggregation state, localized concentration of endogenous quenchers and primary photophysics of the dye, or the intratumoral and intracellular localization of the photosensitizer at the time of activation with light. The purpose of this review is to bring together data relating to the biodistribution and pharmacokinetics of second-generation sensitizers and attempt to correlate this with structural and electronic features of these molecules. As this requires a clear knowledge of photosensitizer structure, only chemically well-characterized compounds are included, e.g. Photofrin and crude sulfonated phthalocyanines have been excluded as they are known to be complex mixtures. Nonporphyrin-based photosensitizers, e.g. rose bengal and the hypericins, have also been omitted to allow meaningful comparisons to be made between different compounds. As the intracellular distribution of photosensitizers to organelles and other subcellular structures can have a large effect on PDT efficacy, a section will be devoted to this topic.
Subject(s)
Photosensitizing Agents/chemistry , Photosensitizing Agents/pharmacokinetics , Animals , Hematoporphyrin Derivative/chemistry , Hematoporphyrin Derivative/pharmacokinetics , Humans , Molecular Structure , Neoplasms/drug therapy , Neoplasms/metabolism , Photochemotherapy , Tissue DistributionABSTRACT
Hexadecafluorinated zinc phthalocyanine (ZnPcF16), an analogue of zinc phthalocyanine (ZnPc) in which all hydrogen atoms have been substituted by fluorine, was prepared as a single isomeric product via the condensation of tetrafluorophthalonitrile with zinc acetate. Fluorination renders the ZnPc soluble in most common solvents. The photodynamic properties and pharmacokinetics of the ZnPcF16 were evaluated in EMT-6 tumour-bearing Balb/c mice using 65Zn-radiolabelled analogues. Both dyes, administered i.v. at 1 mumol kg-1 as Cremophor emulsions, revealed good tumour uptake [approximately 8-9 per cent of the injected dose per g tissue (%IDg-1)] at 24 h post injection (p.i.), with the fluorinated dye reaching higher concentrations (approximately 11%IDg-1) at 48 h p.i. and subsequently higher tumour-blood ratios due to rapid blood clearance. ZnPcF16 at a dose of 5 mumol kg-1 (4.3 mg kg-1) induced complete tumour regression after phototherapy (24 h p.i., 650-700 nm band, 360 J cm-2, 200 mW cm-1). At a dose of 2 mumol kg-1 and phototherapy at 24 h p.i., the tumour volume doubling time increased to 11 days vs 6 days for the control tumours. A similar tumour growth delay was observed when phototherapy was conducted at 48 h or 72 h after dye injection implying that tumour response correlates with tumour dye concentrations rather than serum concentrations. As a result of its low solubility, the administered dose of ZnPc was limited to 1 mumol kg-1 and at this drug level significant tumour response was only observed when the dye was solubilised as the pyridinium salt. Isolation of the neoplastic cells after in vivo dye administration and in vitro exposure to red light followed by a colony formation assay showed that the ZnPcF16 exhibited a 1-2 order of magnitude higher potential for direct cell killing as compared with Photofrin and about a five times lower efficiency than ZnPc. However, all three photosensitisers induced complete occlusion of tumour vasculature immediately after PDT, suggesting that tumour regression mainly resulted from vascular stasis. The ZnPcF16 offers several advantages over ZnPc for clinical applications, including improved solubility in most solvents, resulting in facilitated drug formation, favourable pharmacokinetics as well as the potential use in fluorine magnetic resonance (F-MR) imaging.
Subject(s)
Indoles/pharmacology , Indoles/pharmacokinetics , Neoplasms, Experimental/drug therapy , Neoplasms, Experimental/metabolism , Organometallic Compounds/pharmacology , Organometallic Compounds/pharmacokinetics , Photochemotherapy , Photosensitizing Agents/pharmacology , Photosensitizing Agents/pharmacokinetics , Zinc Radioisotopes , Animals , Emulsions , Isoindoles , Mice , Mice, Inbred BALB C , Polyethylene Glycols/administration & dosage , Tissue Distribution , Zinc CompoundsABSTRACT
Hexadecafluoro zinc phthalocyanine (ZnPcF16), a second generation sensitizer for the photodynamic therapy of cancer, was incorporated in three vehicles: poly(D,L-lactic acid) (PLA) nanoparticles, polyethylene glycol (PEG)-coated nanoparticles and a Cremophor EL (CRM) oil-water emulsion. Nanoparticles were prepared by the salting-out procedure. Biodistribution of the dye was assessed by fluorescence in EMT-6 mammary tumour bearing mice after intravenous injection of 1 mumol kg-1 ZnPcF16. Plain nanoparticles were rapidly retained by the reticuloendothelial system (RES) as reflected by the low area under the blood concentration-time curve (AUC0-168, 57 micrograms h g-1). Little tumour uptake of the dye was observed with this formulation. In contrast, PEG-coated nanoparticles displayed a reduced RES uptake, leading to significantly higher blood levels over an extended period (t1/2 30 h; AUC 0-168 227 micrograms h g-1) and enhanced tumour uptake. At 48 h post injection, tumour to skin and tumour to muscle concentration ratios reached 3.5 and 10.8, respectively. Blood levels of ZnPcF16 after administration as a CRM emulsion decreased faster than with PEG-coated nanoparticles (t1/2 12 h), but since no early liver uptake was observed, the AUC0-168 and the tumour uptake were only slightly lower. However, with the CRM formulation, a late liver uptake was observed, reaching 51% of the injected dose after 7 days.
Subject(s)
Indoles/administration & dosage , Lactic Acid , Mammary Neoplasms, Experimental/drug therapy , Organometallic Compounds/administration & dosage , Photochemotherapy , Radiation-Sensitizing Agents/administration & dosage , Animals , Colloids , Delayed-Action Preparations , Emulsions , Glycerol/analogs & derivatives , Indoles/pharmacology , Injections, Intravenous , Lactates , Male , Mammary Neoplasms, Experimental/metabolism , Mice , Mice, Inbred BALB C , Microspheres , Organometallic Compounds/pharmacology , Polyesters , Polyethylene Glycols , Polymers , Radiation-Sensitizing Agents/pharmacokinetics , Solvents , Tissue DistributionABSTRACT
We evaluated a series of first- and second-generation enzyme-linked immunoassays for anti-HCV and compared the findings to those with two confirmatory assays, the recombinant immunoblot assay and serum HCV RNA, in patients with chronic hepatitis C, primary biliary cirrhosis, and chronic hepatitis B. All second-generation immunoassays had good sensitivities (98-100%). Interestingly, detection of HCV RNA had a sensitivity of only 93%, although it was 100% specific. The recombinant immunoblot assay and a peptide-based immunoassay also had good specificity (97% and 100%, respectively), whereas the second-generation immunoassay based on recombinant proteins had a high rate of false positivity, particularly among patients with primary biliary cirrhosis and hyperglobulinemia (specificity 68%). Thus, the diagnosis of HCV infection appears to require the use of more than one test. Whereas a second-generation enzyme-linked immunoassay can be used as an initial test, a confirmatory test (such as recombinant immunoblot assay or determination of hepatitis C viral RNA) may be required if the diagnosis remains uncertain.
Subject(s)
Hepatitis C/diagnosis , Hepatitis, Chronic/diagnosis , Cohort Studies , False Positive Reactions , Hepacivirus/isolation & purification , Hepatitis B/diagnosis , Hepatitis C/epidemiology , Hepatitis, Chronic/epidemiology , Hepatitis, Chronic/microbiology , Humans , Immunoblotting , Immunoenzyme Techniques , Liver Cirrhosis, Biliary/diagnosis , Polymerase Chain Reaction , RNA, Viral/blood , Sensitivity and SpecificityABSTRACT
Zinc phthalocyanine substituted with four hydroxyl groups attached to the macrocycle, either directly or via spacer chains of three or six carbon atoms, were tested for their photodynamic ability to inactivate Chinese hamster lung fibroblasts (line V-79) in vitro, and to induce regression of EMT-6 tumours grown subcutaneously in Balb/c mice. Their potential to inflict direct cell killing during photodynamic therapy was investigated by examining vascular stasis immediately following photoirradiation using fluorescein as a marker, and also by an in vivo/in vitro EMT-6 cell survival assay. Both of the tetraalkylhydroxy substituted zinc phthalocyanines are effective photodynamic sensitisers in vivo with the tetrapropylhydroxy compound exhibiting about twice the activity of the tetrahexylhydroxy analogue. The differences in activities were accentuated in vitro, the tetrapropylhydroxy compound was two orders of magnitude more potent than the tetrahexylhydroxy analogue in photoinactivating V-79 cells. The tetrahydroxy compound lacking spacer chains failed to exhibit photodynamic activity in either system. Tumour response with the active compounds was preceded by vascular stasis immediate following irradiation which suggests, together with the absence of activity in the in vivo/in vitro assay, that tumour regression involves an indirect response to the photodynamic action rather than direct cell killing. These data demonstrate the importance of the spatial orientation of functional groups around the macrocycle of photosensitisers for their efficacy in the photodynamic therapy of cancer.
Subject(s)
Indoles/pharmacology , Organometallic Compounds/pharmacology , Photochemotherapy/methods , Radiation-Sensitizing Agents/pharmacology , Animals , Coloring Agents/pharmacology , Cricetinae , Cricetulus , Female , Fibroblasts/cytology , Fibroblasts/drug effects , Isoindoles , Lung/cytology , Mammary Neoplasms, Experimental/drug therapy , Mice , Mice, Inbred BALB C , Structure-Activity RelationshipABSTRACT
The effect of human serum components on the photodynamic activity of zinc phthalocyanine (ZnPc) toward Chinese hamster fibroblasts (line V-79) was studied. Photodynamic activities were correlated with cellular uptake of radiolabeled [65Zn]ZnPc, which allowed corrections to be made for the amount of sensitizer present in the cells at the time of irradiation and to express photodynamic efficiencies on a cellular dye concentration basis. All serum components, with the exception of high-density lipoproteins, inhibit uptake of ZnPc by V-79 cells, when compared to incubation of ZnPc with the same cells in serum-free medium. High-density lipoproteins increased ZnPc uptake by 23%, but the photodynamic efficiency corrected for the cellular ZnPc concentration was unaffected. Very low-density lipoprotein and globulins decreased ZnPc cell uptake but likewise did not affect the cellular photodynamic efficiency of the dye. In contrast low-density lipoprotein and albumin, while inhibiting ZnPc cell uptake, increased the cellular photodynamic efficiency of ZnPc, suggesting that these proteins facilitate localization of the dye at cellular targets sensitive to photodynamic damage and vital to cell survival. We conclude from these results that association of ZnPc with serum components can have important, and widely differing, effects on both degree of uptake and cellular distribution of the photosensitizer.
Subject(s)
Blood Proteins/pharmacology , Indoles/metabolism , Indoles/pharmacology , Organometallic Compounds/metabolism , Organometallic Compounds/pharmacology , Photosensitizing Agents/metabolism , Photosensitizing Agents/pharmacology , Zinc/pharmacology , Animals , Biological Transport/drug effects , Cell Line , Cell Survival/drug effects , Cricetinae , Cricetulus , Culture Media, Serum-Free , Humans , Isoindoles , Light , Lipoproteins/blood , Lipoproteins/pharmacology , Lung , Serum Albumin/pharmacology , Serum Globulins/pharmacology , Zinc/metabolism , Zinc CompoundsABSTRACT
Aluminium phthalocyanines substituted to different degrees with hydrophilic sulphonic acid and hydrophobic phthalimidomethyl groups were investigated in vivo as new agents for the photodynamic therapy of malignant tumours. Parameters studied included the photodynamic action on EMT-6 mammary tumours in BALB/c mice, the therapeutic window and the potential for direct cell killing, assayed via an in vivo/in vitro test. Although the efficiency of photoinactivation of the EMT-6 tumour increases by a factor of ten with reduction of the number of sulphonic acid groups from four to two, no further effect was seen with the addition of the hydrophobic phthalimidomethyl groups. Addition of the latter groups however increased the potential for direct cell killing by a factor of two and expanded the therapeutic window by a factor of four, thus improving the usefulness of the dye as a photosensitiser for the photodynamic therapy of cancer.
Subject(s)
Indoles/toxicity , Neoplasms, Experimental/drug therapy , Organometallic Compounds/toxicity , Photochemotherapy/methods , Aluminum , Animals , Coloring Agents , Dose-Response Relationship, Drug , Indoles/administration & dosage , Indoles/chemistry , Male , Mice , Mice, Inbred BALB C , Organometallic Compounds/administration & dosage , Organometallic Compounds/chemistry , Solubility , Structure-Activity RelationshipABSTRACT
The photocytotoxicity of sulfonated phthalimidomethyl aluminum phthalocyanine, a more hydrophobic photosensitizer as compared to phthalocyanine substituted with sulfonate groups only, was investigated. Inclusion of 1-2 phthalimidomethyl groups into disulfonated aluminum phthalocyanine, resulted in increased partition coefficients between n-octanol and water, and a six-fold increase in both cellular uptake and photocytotoxicity towards Chinese hamster lung fibroblast cells (line V-79). Reducing the number of phthalimidomethyl groups, or increasing the degree of sulfonation, lead to a decrease in the partition coefficient, cellular uptake, and phototoxicity. The quantum yield of singlet oxygen was comparable for all dyes tested in this series, indicating that no significant change in this photophysical parameter resulted from phthalimidomethylation. These results suggest that the addition of 1-2 phthalimidomethyl groups to disulfonated aluminum phthalocyanine improves cellular uptake, but, as the relative efficiency of cell killing was not effected, the intracellular distribution on photosensitive molecules may not be modified.
Subject(s)
Cell Survival/radiation effects , Indoles/pharmacology , Organometallic Compounds/pharmacology , Phthalimides/pharmacology , Radiation-Sensitizing Agents/pharmacology , Animals , Cell Line , Cell Survival/drug effects , Indoles/metabolism , Light , Organometallic Compounds/metabolism , Phthalimides/metabolism , Structure-Activity RelationshipABSTRACT
To evaluate the biodistribution and tumor uptake of chlorogallium tetraoctadecycloxy phthalocyanine, a potential new drug for the photodynamic therapy of cancer, we prepared the radioactive 67Ga-labeled complex and its water soluble sulfonated derivative. The non-sulfonated dye was obtained by condensation of octadecyloxyphthalonitrile in the presence of a mixture of 67Ga and 69Ga chloride. The sulfonated derivative was obtained by treatment of the condensation product with oleum. As singlet molecular oxygen has been implicated in the photodynamic action of phthalocyanines (Pcs), the quantum yield of singlet oxygen (phi delta) was evaluated for chlorogallium tetraoctadecyloxy Pc, and also its zinc, aluminum and metal free analogues. After intraperitoneal administration of the non-sulfonated dye into RIF tumor bearing C3H mice, a very high 67Ga-uptake was observed in the spleen, while tumor radioactivity remained low during the 3 day study. The in vivo stability of the 67Ga-phthalocyanine complex was confirmed by comparing the distribution pattern with that of 67Ga-citrate, which proved to be significantly different. Intravenous injection of the sulfonated dye resulted in an overall lowering of 67Ga-uptake by most tissues, particularly in the spleen, while tumor radioactivities were slightly higher. These data suggest that amphiphilic photosensitizers, containing both polar sulfonate groups and long aliphatic substituents, exhibit the best distribution pattern for both imaging and photodynamic therapy of tumors.
Subject(s)
Gallium Radioisotopes , Indoles/pharmacology , Neoplasms, Experimental/metabolism , Photochemotherapy , Animals , Free Radicals , Indoles/therapeutic use , Intestinal Absorption , Mice , Mice, Inbred C3H , Neoplasms, Experimental/drug therapy , Oxygen/metabolism , Sulfuric Acids/chemistryABSTRACT
It has been suggested recently that neither an ester nor an ether linkage was present in the tumour-localising component of Photofrin II (PFII) (a commercial preparation of DHE). We now report that our chemical and spectroscopic studies, establish the presence of an ester linkage with the possibility that an ether linkage may also be involved.