ABSTRACT
Respiratory syncytial virus (RSV) infection is a leading cause of severe lower respiratory tract illness in young infants, the elderly and immunocompromised individuals. We demonstrate here that the co-inhibitory molecule programmed cell death 1 (PD-1) is selectively upregulated on T cells within the respiratory tract during both murine and human RSV infection. Importantly, the interaction of PD-1 with its ligand PD-L1 is vital to restrict the pro-inflammatory activities of lung effector T cells in situ, thereby inhibiting the development of excessive pulmonary inflammation and injury during RSV infection. We further identify that PD-L1 expression on lung inflammatory dendritic cells is critical to suppress inflammatory T-cell activities, and an interferon-STAT1-IRF1 axis is responsible for increased PD-L1 expression on lung inflammatory dendritic cells. Our findings suggest a potentially critical role of PD-L1 and PD-1 interactions in the lung for controlling host inflammatory responses and disease progression in clinical RSV infection.
Subject(s)
B7-H1 Antigen/metabolism , Dendritic Cells/immunology , Dendritic Cells/metabolism , Respiratory Syncytial Virus Infections/immunology , Respiratory Syncytial Virus Infections/metabolism , Respiratory Syncytial Viruses/immunology , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , Animals , B7-H1 Antigen/antagonists & inhibitors , B7-H1 Antigen/genetics , Disease Models, Animal , Disease Progression , Gene Expression , Host-Pathogen Interactions , Humans , Interferon Regulatory Factor-1/metabolism , Interferons/metabolism , Lymphocyte Activation/immunology , Mice , Programmed Cell Death 1 Receptor/metabolism , Respiratory Syncytial Virus Infections/genetics , Respiratory Syncytial Virus Infections/pathology , STAT1 Transcription Factor/metabolismABSTRACT
Tissue injury is a common sequela of acute virus infection localized to a specific organ such as the lung. Tissue injury is an immediate consequence of infection with lytic viruses. It can also result from the direct destruction of infected cells by effector CD8(+) T lymphocytes and indirectly through the action of the T cell-derived proinflammatory cytokines and recruited inflammatory cells on infected and uninfected tissue. We have examined CD8(+) T cell-mediated pulmonary injury in a transgenic model in which adoptively transferred, virus-specific cytotoxic T lymphocytes (CTLs) produce lethal, progressive pulmonary injury in recipient mice expressing the viral target transgene exclusively in the lungs. We have found that over the 4-5 day course of the development of lethal pulmonary injury, the effector CTLs, while necessary for the induction of injury, are present only transiently (24-48 h) in the lung. We provide evidence that the target of the antiviral CD8(+) T cells, the transgene expressing type II alveolar cells, are not immediately destroyed by the effector T cells. Rather, after T cell-target interaction, the type II alveolar cells are stimulated to produce the chemokine monocyte chemoattractant protein 1. These results reinforce the concept that, in vivo, the cellular targets of specific CTLs may participate directly in the development of progressive tissue injury by activating in response to interaction with the T cells and producing proinflammatory mediators without sustained in vivo activation of CD8(+) T cell effectors.
Subject(s)
CD8-Positive T-Lymphocytes/immunology , Cytotoxicity, Immunologic , Lung/immunology , T-Lymphocyte Subsets/immunology , Adoptive Transfer , Animals , Chemokine CCL2/immunology , Hemagglutinins, Viral/immunology , Influenza A virus/immunology , Lung/pathology , Mice , Mice, Transgenic , T-Lymphocytes, Regulatory/immunologyABSTRACT
CTL play a major role in the clearance of respiratory syncytial virus (RSV) during experimental pulmonary infection. The fusion (F) glycoprotein of RSV is a protective Ag that elicits CTL and Ab response against RSV infection in BALB/c mice. We used the strategy of screening a panel of overlapping synthetic peptides corresponding to the RSV F protein and identified an immunodominant H-2K(d)-restricted epitope (F(85-93); KYKNAVTEL) recognized by CD8(+) T cells from BALB/c mice. We enumerated the F-specific CD8(+) T cell response in the lungs of infected mice by flow cytometry using tetramer staining and intracellular cytokine synthesis. During primary infection, F(85-93)-specific effector CD8(+) T cells constitute approximately 4.8% of pulmonary CD8(+) T cells at the peak of the primary response (day 8), whereas matrix 2-specific CD8(+) T cells constituted approximately 50% of the responding CD8(+) T cell population in the lungs. When RSV F-immune mice undergo a challenge RSV infection, the F-specific CD8(+) T cell response is accelerated and dominates, whereas the primary response to the matrix 2 epitope in the lungs is reduced by approximately 20-fold. In addition, we found that activated F-specific effector CD8(+) T cells isolated from the lungs of RSV-infected mice exhibited a lower than expected frequency of IFN-gamma-producing CD8(+) T cells and were significantly impaired in ex vivo cytolytic activity compared with competent F-specific effector CD8(+) T cells generated in vitro. The significance of these results for the regulation of the CD8(+) T cell response to RSV is discussed.
Subject(s)
Antigens, Viral/immunology , Lung/immunology , Respiratory Syncytial Virus Infections/immunology , T-Lymphocytes, Cytotoxic/immunology , Viral Proteins/immunology , Animals , Epitope Mapping , Female , H-2 Antigens/immunology , Immunodominant Epitopes/immunology , Lung/cytology , Mice , Peptide Fragments/immunology , Spleen/cytology , Spleen/immunologyABSTRACT
Vaccination with the respiratory syncytial virus (RSV) attachment (G) protein results in immune-mediated lung injury after natural RSV infection with pathogenic features characteristic of an exaggerated Th2 response. Here we demonstrate that approximately half of the CD4(+) T cells infiltrating the lungs of G-primed mice utilize a single V beta gene (V beta 14) with remarkably limited CDR3 diversity. Furthermore, elimination of these V beta 14-bearing CD4(+) T cells in vivo abolishes the type 2-like pulmonary injury. These results suggest that a novel subset of CD4(+) T cells may be crucial in the development of pathology during human RSV infection and that genetic or environmental factors prior to or at the time of G antigen exposure may affect the commitment of this discrete antigen-specific T cell subset to Th2 differentiation.
Subject(s)
Antigens, Viral/immunology , CD4-Positive T-Lymphocytes/immunology , Respiratory Syncytial Virus Infections/immunology , Viral Proteins/immunology , Animals , Cells, Cultured , Complementarity Determining Regions/genetics , Female , Immunoglobulin Variable Region/genetics , Immunologic Memory , Lung/immunology , Lymphocyte Activation , Lymphocyte Depletion , Mice , Mice, Inbred BALB C , Pulmonary Eosinophilia/immunology , Receptors, Antigen, T-Cell, alpha-beta/genetics , Respiratory Syncytial Virus Infections/pathology , Respiratory Syncytial Viruses/immunology , T-Lymphocyte Subsets/classification , Th2 Cells/immunologyABSTRACT
Physically distinct cholesterol/sphingolipid-rich plasma membrane microdomains, so-called lipid rafts, have been recognized to play an important regulatory role in various cellular processes, from membrane trafficking to signal transduction, in a number of cell types. We report here that the ability of TCR on activated, functional CD8+ T lymphocytes to efficiently bind MHC class I tetramer complexes is dependent on the integrity of lipid rafts on the T lymphocyte membrane. We further provide evidence that TCR interact (associate) with lipid raft elements on the T cell surface before receptor engagement and that the topological arrangement of TCR on the cell surface is likewise influenced by lipid raft integrity.
Subject(s)
CD8-Positive T-Lymphocytes/metabolism , Histocompatibility Antigens Class I/metabolism , Membrane Microdomains/physiology , Receptors, Antigen, T-Cell/metabolism , beta-Cyclodextrins , Animals , Binding Sites/drug effects , Binding Sites/immunology , CD8-Positive T-Lymphocytes/drug effects , CD8-Positive T-Lymphocytes/immunology , Cell Line , Cell Membrane/drug effects , Cell Membrane/immunology , Cell Membrane/metabolism , Cells, Cultured , Clone Cells , Cyclodextrins/pharmacology , Epitopes, T-Lymphocyte/immunology , Hemagglutinin Glycoproteins, Influenza Virus/immunology , Lymphocyte Activation , Membrane Microdomains/metabolism , Mice , Mice, Inbred BALB C , Peptide Fragments/immunology , T-Lymphocytes, Cytotoxic/drug effects , T-Lymphocytes, Cytotoxic/immunology , T-Lymphocytes, Cytotoxic/metabolismABSTRACT
Hepatitis C virus (HCV) is an important human pathogen that is remarkably efficient at establishing persistent infection. The HCV core protein is the first protein expressed during the early phase of HCV infection. Our previous work demonstrated that the HCV core protein suppresses host immune responses, including anti-viral cytotoxic T-lymphocyte responses in a murine model. To investigate the mechanism of HCV core-mediated immunosuppression, we searched for host proteins capable of associating with the core protein using a yeast two-hybrid system. Using the core protein as bait, we screened a human T cell-enriched expression library and identified a gene encoding the gC1q receptor (gC1qR). C1q is a ligand of gC1qR and is involved in the early host defense against infection. Like C1q, HCV core can inhibit T-cell proliferative responses in vitro. This core-induced anti-T-cell proliferation is reversed by addition of anti-gC1qR Ab in a T-cell proliferation assay. Furthermore, biochemical analysis of the interaction between core and gC1qR indicates that HCV core binds the region spanning amino acids 188 to 259 of gC1qR, a site distinct from the binding region of C1q. The inhibition of T-cell responsiveness by HCV core may have important implications for HCV persistence in humans.
Subject(s)
Complement C1q/immunology , Hepacivirus/immunology , Hyaluronan Receptors , Membrane Glycoproteins , Receptors, Complement/immunology , T-Lymphocytes, Cytotoxic/immunology , Viral Core Proteins/immunology , Animals , Binding Sites , Carrier Proteins , Cell Division , Cell Line , Cells, Cultured , Chromosome Mapping , Hepacivirus/genetics , Humans , Mice , Mitochondrial Proteins , Receptors, Complement/genetics , T-Lymphocytes, Cytotoxic/cytology , Viral Core Proteins/geneticsABSTRACT
BALB/c mice immunized with a vaccinia virus expressing the attachment (G) glycoprotein of respiratory syncytial virus (RSV) develop a virus-specific CD4(+) T cell response that consists of a mixture of Th1 and Th2 CD4(+) T cells following intranasal infection with live RSV. Recent work has shown that both Th1 and Th2 CD4(+) T cells are elicited to a single region comprising aa 183-197 of the G protein. To more precisely define the CD4(+) T cell epitope(s) contained within this region, we created a panel of amino- and carboxyl-terminal truncated as well as single alanine-substituted peptides spanning aa 183-197. These peptides were used to examine the ex vivo cytokine response of memory effector CD4(+) T cells infiltrating the lungs of G-primed RSV-infected mice. Analysis of lung-derived memory effector CD4(+) T cells using intracellular cytokine staining and/or ELISA of effector T cell culture supernatants revealed a single I-E(d)-restricted CD4(+) T cell epitope with a core sequence mapping to aa 185-193. In addition, we examined the T cell repertoire of the RSV G peptide-specific CD4(+) T cells and show that the CD4(+) T cells directed to this single immunodominant G epitope use a restricted range of TCR Vss genes and predominantly express Vss14 TCR.
Subject(s)
Epitopes, T-Lymphocyte/immunology , Glycoproteins/immunology , HN Protein , Immunodominant Epitopes/immunology , Respiratory Syncytial Virus, Human/immunology , Th1 Cells/immunology , Th2 Cells/immunology , Viral Proteins/immunology , Alanine/genetics , Amino Acid Sequence , Animals , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/metabolism , CD4-Positive T-Lymphocytes/virology , Cells, Cultured , Epitopes, T-Lymphocyte/analysis , Epitopes, T-Lymphocyte/metabolism , Female , Glycoproteins/biosynthesis , Glycoproteins/genetics , Immunodominant Epitopes/analysis , Immunodominant Epitopes/metabolism , Immunologic Memory , Immunophenotyping , Lung/immunology , Lung/pathology , Lung/virology , Lymphocyte Count , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Mutagenesis, Site-Directed , Receptors, Antigen, T-Cell, alpha-beta/metabolism , Respiratory Syncytial Virus Infections/immunology , Respiratory Syncytial Virus Infections/virology , Th1 Cells/metabolism , Th1 Cells/virology , Th2 Cells/metabolism , Th2 Cells/virology , Vaccinia virus/genetics , Vaccinia virus/immunology , Viral Envelope Proteins , Viral Proteins/biosynthesis , Viral Proteins/geneticsABSTRACT
Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that persistently infects 85% of the adult population worldwide. In this report, we examine the proliferative response and cytokine secretion profile of CD4(+) T lymphocytes from a panel of unrelated EBV-positive donors against two EBV latent antigens, EBNA1 and EBNA3C. Substantial proliferative responses by CD4(+) lymphocytes were demonstrated to both antigens in multiple, randomly selected donors. Surprisingly, we observed a striking and consistent difference in cytokine response to EBNA1 and EBNA3C. EBNA1-specific CD4(+) T lymphocytes from multiple unrelated donors preferentially produced type 2-like cytokines in response to antigenic stimulation, while the response to EBNA3C was a characteristic type 1 response. The implications of these findings for EBV persistence and the development of EBV-associated malignancies are discussed.
Subject(s)
CD4-Positive T-Lymphocytes/immunology , Cytokines/biosynthesis , Epstein-Barr Virus Nuclear Antigens/immunology , Herpesvirus 4, Human/immunology , Th2 Cells/immunology , Adult , Antigens, Viral/genetics , Antigens, Viral/immunology , Blood Donors , Cells, Cultured , Dose-Response Relationship, Drug , Humans , Infectious Mononucleosis/immunology , Infectious Mononucleosis/virology , Lymphocyte Activation , Recombinant Proteins/immunology , Recombinant Proteins/metabolism , Th1 Cells/immunologyABSTRACT
CD8(+) cytotoxic T lymphocytes (CTLs) recognize antigen in the context of major histocompatibility complex (MHC) class I molecules. Class I epitopes have been classified as dominant or subdominant depending on the magnitude of the CTL response to the epitope. In this report, we have examined the in vitro memory CTL response of H-2(d) haplotype murine CD8(+) T lymphocytes specific for a dominant and subdominant epitope of influenza hemagglutinin using activation marker expression and staining with soluble tetrameric MHC-peptide complexes. Immune CD8(+) T lymphocytes specific for the dominant HA204-210 epitope give rise to CTL effectors that display activation markers, stain with the HA204 tetramer, and exhibit effector functions (i.e., cytolytic activity and cytokine synthesis). In contrast, stimulation of memory CD8(+) T lymphocytes directed to the subdominant HA210-219 epitope results in the generation of a large population of activated CD8(+) T cells that exhibit weak cytolytic activity and fail to stain with the HA210 tetramer. After additional rounds of restimulation with antigen, the HA210-219-specific subdominant CD8(+) T lymphocytes give rise to daughter cells that acquire antigen-specific CTL effector activity and transition from a HA210 tetramer-negative to a tetramer-positive phenotype. These results suggest a novel mechanism to account for weak CD8(+) CTL responses to subdominant epitopes at the level of CD8(+) T lymphocyte differentiation into effector CTL. The implications of these findings for CD8(+) T lymphocyte activation are discussed.
Subject(s)
Antigens, Viral , CD8-Positive T-Lymphocytes/cytology , CD8-Positive T-Lymphocytes/immunology , T-Lymphocytes, Cytotoxic/immunology , Animals , Cell Differentiation , Cell Line , Clonal Anergy , Cytotoxicity, Immunologic , Epitopes/chemistry , Female , H-2 Antigens/chemistry , Hemagglutinin Glycoproteins, Influenza Virus , Immunodominant Epitopes/chemistry , Lymphocyte Activation , Mice , Mice, Inbred BALB C , Protein Structure, Quaternary , T-Lymphocytes, Cytotoxic/cytologyABSTRACT
The Ag receptors on CD8+ CTL recognize foreign antigenic peptides associated with cell surface MHC class I molecules. Peptides derived from self proteins are also normally presented by MHC class I molecules. Here we report that an H-2Kd-restricted murine CD8+ CTL clone directed to an influenza hemagglutinin epitope can recognize a peptide derived from the murine mitochondrial aconitase enzyme in association with H-2Kd molecules. Surprisingly, this self peptide is not normally displayed on the cell surface associated with the restricting MHC class I molecule. Several lines of evidence suggest that this self peptide, although requiring association with the Kd molecule for CTL recognition, is not associated with this or other MHC class I allele under physiologic conditions in intact cells. Rather, it is sequestered in the cytoplasm associated with a carrier protein and is released only upon cell disruption. These results suggest a means of restricting the entry of self peptide into the class I pathway. In addition, this finding raises the possibility that self peptides sequestered within the cell can, after release from damaged cells, interact with MHC class I molecules on bystander cells and trigger autoimmune injury by virus-specific CTLs during viral infection.
Subject(s)
Epitopes, T-Lymphocyte/metabolism , Influenza A virus/immunology , Oligopeptides/immunology , Oligopeptides/metabolism , T-Lymphocytes, Cytotoxic/immunology , Aconitate Hydratase/immunology , Aconitate Hydratase/isolation & purification , Aconitate Hydratase/metabolism , Amino Acid Sequence , Animals , Antigen Presentation , Carrier Proteins/immunology , Carrier Proteins/metabolism , Clone Cells , H-2 Antigens/immunology , H-2 Antigens/metabolism , Hemagglutinin Glycoproteins, Influenza Virus/immunology , Hemagglutinin Glycoproteins, Influenza Virus/metabolism , Mice , Mice, Inbred BALB C , Mice, Inbred C3H , Mice, Inbred C57BL , Mice, Knockout , Mitochondria/enzymology , Molecular Sequence Data , Subcellular Fractions/immunology , Subcellular Fractions/metabolism , T-Lymphocytes, Cytotoxic/metabolism , T-Lymphocytes, Cytotoxic/virology , Tumor Cells, CulturedABSTRACT
In BALB/c mice, sensitization to respiratory syncytial virus (RSV) attachment (G) glycoprotein leads to the development of lung eosinophilia upon challenge infection with RSV, a pathology indicative of a strong in vivo induction of a Th-2-type response. In this study, we found that a strong, RSV G-specific, Th-1-type cytokine response occurred simultaneously with a Th-2-type response in G-primed mice after RSV challenge. Both Th-1 and Th-2 effector CD4(+) T cells recognized a single immunodominant site on this protein, implying that the differentiation of memory CD4(+) T cells along the Th-1 or Th-2 effector pathway was independent of the epitope specificity of the T cells. A similar observation was made in G-primed H-2(b) haplotype mice after RSV challenge, further suggesting that this process is not dependent on the peptide epitope presented. On the other hand, genes mapping to loci outside of the major histocompatibility complex region are crucial regulators of the development of a Th-2-type response and lung eosinophilia. The implication of these findings for the immune mechanisms underlying the pathogenesis of RSV is discussed.
Subject(s)
Epitopes, T-Lymphocyte/immunology , HN Protein , Major Histocompatibility Complex/immunology , Respiratory Syncytial Virus Infections/immunology , Respiratory Syncytial Virus, Human/immunology , Th1 Cells/immunology , Th2 Cells/immunology , Viral Proteins/immunology , Animals , Cytokines/biosynthesis , Eosinophilia/immunology , Female , Humans , Lung/immunology , Lung/pathology , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Peptides/immunology , Tumor Cells, Cultured , Viral Envelope ProteinsABSTRACT
CD8(+) T cells appear to play an important pathophysiologic role in many inflammatory lung diseases. The primary effector function of this T-cell subset is cytolysis of virus-infected cells, and it is widely believed that there are two primary molecular mechanisms by which this occurs: the perforin/granzyme-mediated pathway of cytolysis, and the Fas ligand (FasL)-Fas (CD95/APO-1) pathway of induction of target-cell apoptosis. This conclusion is based primarily on data obtained with hematopoetic cell lines as target cells. There is also a growing body of evidence that Fas is involved in the transduction of apoptotic signals in a variety of inflammatory disease states, particularly involving the liver and the lung. In the study reported here we took advantage of a novel in vitro assay to directly assess the effector mechanisms employed in CD8(+) T-cell-mediated cytolysis of alveolar epithelial cells. We present evidence that FasL-induced, Fas-mediated apoptosis does not directly contribute to T-cell-mediated cytolysis of alveolar epithelial-derived cells, even though Fas is expressed and functional on these cells. We also demonstrated that the perforin-independent cytolytic activity of CD8(+) T cells against alveolar epithelial-derived cells is explained entirely by tumor necrosis factor-alpha (TNF-alpha), which is expressed on CD8(+) T cells. Furthermore, we show that bystander cytolysis of alveolar epithelial-derived cells by antiviral CD8(+) T cells is entirely perforin-independent. This activity is mediated exclusively by TNF-alpha. Both alveolar epithelial-derived cells and primary murine type II cells show susceptibility to apoptosis triggered by soluble TNF-alpha, without the need for transcriptional or translational inhibition. We also confirmed the resistance of alveolar type II cells to FasL in vivo by performing adoptive transfer of perforin-deficient antiviral CD8(+) T cells into transgenic mice expressing a target antigen in type II epithelial cells. Significant lung injury developed in the transgenic CD8(+) T-cell recipients, whether or not Fas was expressed in these animals. Furthermore, preincubation of the T cells with antibody to TNF-alpha completely abolished the injury. These results suggest that alveolar epithelial cells are relatively sensitive to T cell-triggered, TNF-alpha-mediated apoptosis, and resistant to apoptosis triggered by FasL. These observations may have important ramifications for understanding of the pathophysiology of interstitial and inflammatory lung diseases.
Subject(s)
CD8-Positive T-Lymphocytes/immunology , Cytotoxicity, Immunologic , Membrane Glycoproteins/physiology , Pulmonary Alveoli/immunology , Tumor Necrosis Factor-alpha/physiology , Animals , Apoptosis , Cell Line , Epithelial Cells/immunology , Fas Ligand Protein , Mice , Mice, Inbred BALB C , Mice, Transgenic , Perforin , Pore Forming Cytotoxic Proteins , Pulmonary Alveoli/cytologyABSTRACT
CD8(+) T cells infiltrate the lung in many clinical conditions, particularly in interstitial lung disease. The role(s) that CD8(+) T cells might be playing in the pathogenesis of inflammatory lung disease is unclear at present, as is the direct contribution of CD8(+) T cell effector activities to lung injury. This report describes a transgenic model used to evaluate the impact, on respiratory structure and function, of CD8(+) T lymphocyte recognition of a target antigen expressed endogenously in alveolar epithelial cells. We found that adoptive transfer of cloned CD8(+) cytotoxic T lymphocytes (CTLs) specific for an alveolar neo-antigen (influenza hemagglutinin) leads to progressive lethal injury in transgenic mice, which dramatically affects lung structure and function. Transgenic recipients of CD8(+) CTLs exhibited tachypnea and progressive weight loss, becoming moribund over a period of several days. Concomitantly, the animals developed a progressive interstitial pneumonitis characterized initially by lymphocytic infiltration of alveolar walls and spaces, followed by an exuberant mononuclear cell infiltration that correlated with restrictive pulmonary mechanics and a progressive diffusion impairment. These results indicate that antigen-specific CD8(+) T cell recognition of an alveolar epithelial "autoantigen" is, in and of itself, sufficient to trigger an inflammatory cascade that results in the histological and physiological manifestations of interstitial pneumonia.
Subject(s)
Lung Diseases/immunology , Lung/immunology , T-Lymphocytes, Cytotoxic/immunology , Adoptive Transfer , Animals , Autoantigens/immunology , Cell Line , Hemagglutinin Glycoproteins, Influenza Virus/genetics , Hemagglutinin Glycoproteins, Influenza Virus/immunology , Lung/pathology , Mice , Mice, Inbred BALB C , Mice, TransgenicABSTRACT
T lymphocytes play a pivotal role in the immune response during viral infections. In a murine model of experimental respiratory syncytial virus (RSV) infection, mice sensitized to either of the two major glycoproteins of RSV develop distinct patterns of cytokine secretion and lung inflammation upon subsequent RSV infection. Mice sensitized to RSV-G (attachment) glycoprotein exhibit a strong interleukin (IL)-4 and IL-5 response and develop pulmonary eosinophilia, whereas mice sensitized to RSV-F (fusion) glycoprotein develop a predominantly T helper cell (Th)1 response and pulmonary inflammation characterized by mononuclear cell infiltration. In this study, we examined the potential role of virus-specific CD8+ T cytolytic T cells on the differentiation and activation of functionally distinct CD4+ T cells specific to these viral glycoproteins. Mice primed with recombinant vaccinia virus expressing RSV-F glycoprotein mounted a strong RSV-specific, MHC class I-restricted cytolytic response, whereas priming with recombinant vaccinia virus expressing RSV-G glycoprotein failed to elicit any detectable cytolytic response. Priming for a RSV-specific CD8+ T cell response, either with a recombinant vaccinia virus expressing RSV-G glycoprotein in which a strong CD8+ T cell epitope from RSV-M2 (matrix) protein has been inserted or with a combination of vaccinia virus expressing the matrix protein and the RSV-G glycoprotein, suppressed the eosinophil recruitment into the lungs of these mice upon subsequent challenge with RSV. This reduction in pulmonary eosinophilia correlated with the suppression of Th2 type cytokine production. The importance of CD8+ T cells in this process was further supported by the results in CD8+ T cell deficient, beta 2 microglobulin KO mice. In these mice, priming to RSV-F glycoprotein (which in normal mice primed for a strong cytolytic response and a pulmonary infiltrate consisting primarily of mononuclear cells on RSV challenge) resulted in the development of marked pulmonary eosinophilia that was not seen in mice with an intact CD8+ T cell compartment. These results indicate that CD8+ T cells may play an important role in the regulation of the differentiation and activation of Th2 CD4+ T cells as well as the recruitment of eosinophils into the lungs during RSV infection.
Subject(s)
CD8-Positive T-Lymphocytes/virology , Cytokines/metabolism , Down-Regulation/immunology , HN Protein , Pulmonary Eosinophilia/immunology , Respiratory Syncytial Virus Infections/immunology , Respiratory Syncytial Viruses/immunology , Th2 Cells/metabolism , Amino Acid Sequence , Animals , CD8-Positive T-Lymphocytes/immunology , Cytokines/biosynthesis , Cytotoxicity, Immunologic , Female , Mice , Mice, Inbred BALB C , Mice, Knockout , Molecular Sequence Data , Molecular Weight , Pulmonary Eosinophilia/pathology , Pulmonary Eosinophilia/virology , Recombinant Fusion Proteins/administration & dosage , Recombinant Fusion Proteins/immunology , Respiratory Syncytial Virus Infections/genetics , Respiratory Syncytial Virus Infections/pathology , Spleen/immunology , Spleen/metabolism , Spleen/virology , Th2 Cells/virology , Viral Envelope Proteins , Viral Matrix Proteins/administration & dosage , Viral Matrix Proteins/genetics , Viral Proteins/administration & dosage , Viral Proteins/geneticsABSTRACT
Mice sensitized to the G (attachment) or F (fusion) glycoproteins of respiratory syncytial virus (RSV) expressed different patterns of cytokine production and lung pathology when challenged by intranasal infection with RSV. Five days after challenge, mice sensitized to G glycoprotein produced high levels of interleukin-4 (IL-4) and IL-5 in the lungs and spleens and developed extensive pulmonary eosinophilia, while mice sensitized to F glycoprotein produced IL-2 and developed a mononuclear cell infiltration. Memory lymphocytes isolated 2 weeks after intranasal challenge of mice primed to the G or F glycoprotein secreted only IL-2 and gamma interferon (IFN-gamma) when stimulated with RSV. IL-4 and IL-5 production characteristic of Th2-type effectors in the lung was observed only after multiple rounds of in vitro stimulation of RSV G-specific memory T lymphocytes with antigen. Also IFN-gamma production appeared to play only a minor role in the expression of pulmonary pathology characteristic of Th1 or Th2 T-lymphocyte responses, because mice genetically deficient in IFN-gamma production by gene disruption displayed the same pattern of pulmonary inflammation to RSV infection after priming to RSV F or G as conventional mice. These results suggest that effector T lymphocytes exhibit a different pattern of cytokine production than memory T-lymphocyte precursors precommitted to a Th1 or Th2 pattern of differentiation. Furthermore, these observations raise the possibility that the cytokine response of human memory T lymphocytes after a single exposure to antigen in vitro may not accurately reflect the cytokine response of differentiated effector T lymphocytes at the site of infection in vivo.
Subject(s)
Antigens, Viral/immunology , Cytokines/immunology , HN Protein , Respiratory Syncytial Virus Infections/immunology , Respiratory Syncytial Viruses/immunology , T-Lymphocytes/immunology , Viral Proteins/immunology , Animals , CD4-Positive T-Lymphocytes , Eosinophils/immunology , Female , Immunologic Memory , Interferon-gamma/immunology , Lung/pathology , Male , Mice , Mice, Inbred BALB C , Respiratory Syncytial Virus Infections/pathology , Viral Envelope ProteinsABSTRACT
In the adaptive immune response to most viruses, both the cellular and humoral arms of the immune system play complementary roles in eliminating virus and virus-infected cells and in promoting recovery. To evaluate the relative contribution of CD4+ and CD8+ effector T lymphocytes in virus clearance and recovery, we have examined the host response to lethal type A influenza virus infection in B lymphocyte-deficient mice with a targeted disruption in the immunoglobulin mu heavy chain. Our results indicate that naive B cell-deficient mice have a 50- 100-fold greater susceptibility to lethal type A influenza virus infection than do wild type mice. However, after priming with sublethal doses of influenza, immune B cell-deficient animals show an enhanced resistance to lethal virus infection. This finding indicates that an antibody-independent immune-mediated antiviral mechanism accounts for the increased resistance to lethal virus challenge. To assess the contribution of influenza-specific CD4+ and CD8+ effector T cells in this process, defined clonal populations of influenza-specific CD4+ and CD8+ effector T cells were adoptively transferred into lethally infected B cell-deficient mice. Cloned CD8+ effectors efficiently promoted recovery from lethal infection, whereas cloned CD4+ T cells conferred only partial protection. These results suggest that memory T lymphocytes can act independently of a humoral immune response in order to confer resistance to influenza infection in immune individuals. The potential implications of these results for vaccination against human influenza infection are discussed.
Subject(s)
B-Lymphocytes/physiology , Immunity, Innate/immunology , Immunologic Memory/physiology , Influenza, Human/immunology , Animals , B-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Female , Humans , Influenza A virus , Male , Mice , Mice, Inbred C57BL , Mice, KnockoutABSTRACT
Cytotoxic T lymphocytes (CTL) recognize short antigenic peptides in association with class I MHC molecules at the cell surface. Newly synthesized viral polypeptides are processed in the cytoplasm and the fragments of antigen are transported into the endoplasmic reticulum (ER) via a peptide transporter where they complex with nascent class I molecules. The peptide-MHC complex is transported to the cell surface and presented to CTL. Sequence analysis of endogenously expressed, MHC-associated self or viral antigens indicates that the naturally processed peptides bound to class I MHC molecules are in general 9 +/- 1 residues long. Peptides bound to specific class I MHC molecules have in common allele-specific motifs of conserved residues. The motif for the class I Kd molecules has been shown to be nine or 10 residues with the sequence X-Tyr-(X)6-I/L or X-Tyr-(X)7-I/L. The Tyr residue at the second position and the I/L residue at the ninth position are allele-specific anchor residues which appear to be required for binding of the peptide to Kd. To examine the stringency of the requirement for Tyr at the second position, we have performed saturation mutagenesis of a minigene encoding the class I Kd-restricted influenza HA210-219 site at the Tyr residue 211. A series of 10 mutants was tested for effects on target-cell sensitization. Most amino acid substitutions for the Tyr residue resulted in a loss of endogenous peptide recognition by HA210-219 reactive CTL, consistent with the critical role of the Tyr at the second position for interaction with Kd molecules. One mutant gene-product encoding a His substitution for the Tyr residue was recognized by CTL. However, the corresponding synthetic peptide containing a His substitution at the dominant anchor position bound only weakly to Kd, and target cells treated with the peptide were poorly recognized by CTL. The endogenous His-containing peptide was also less stably associated with class I MHC Kd molecules at the cell surface than the wild-type Tyr peptide. These data indicate that endogenous antigenic peptides may bind newly-synthesized class I MHC molecules in the ER more efficiently than fully formed class I molecules at the cell surface and that endogenous peptides may dissociate from class I MHC molecules at different rates. The implication of these findings for CTL recognition and epitope mapping are discussed.
Subject(s)
Antigen Presentation/immunology , CD8-Positive T-Lymphocytes/immunology , Epitopes/immunology , Amino Acid Sequence , Animals , Cell Line , Chick Embryo , Cricetinae , Major Histocompatibility Complex/immunology , Molecular Sequence Data , Peptide Biosynthesis , Peptides/immunology , Tumor Cells, CulturedABSTRACT
T cell interaction with antigenic peptides leads to signal transduction and activation events in the effector cells. Recent studies of T cell responses to subtle variants of antigenic peptides can lead to alterations in the activation state of T cells. A variety of physiological roles for altered peptide ligands have recently been postulated, and their potential therapeutic applications have generated considerable interest. This review summarizes progress made in understanding the T cell signal transduction pathways and the nature of T cell responses to altered peptide ligands. Our recent observation of a self peptide as a partial agonist for a cytotoxic T cell clone directed to a foreign antigen suggests that naturally occurring altered peptide ligands may be important in regulating T cell mediated immune response.
Subject(s)
Autoantigens/immunology , Ligands , Lymphocyte Activation/immunology , Peptide Fragments/immunology , Signal Transduction/immunology , T-Lymphocytes/immunology , Animals , Apoptosis/immunology , Exocytosis/immunology , Humans , Immunity, Cellular/immunology , Receptors, Antigen, T-Cell/immunology , T-Lymphocytes, Cytotoxic/immunology , fas Receptor/immunologyABSTRACT
The A/Japan/57 influenza hemagglutinin (HA) was expressed in BALB/c mice under the transcriptional control of the surfactant protein C (SP-C) promoter, resulting in expression of HA in type II alveolar epithelial cells, as well as low level variable expression in other tissues, including the thymus in some of the founder lines. Transgenic animals were able to recover from infection with A/Japan/57 influenza, and they were able to mount antibody responses to A/Japan/57 HA in titers similar to wild type. We therefore tested their CD4+ T lymphocyte responses to HA and found them to be similar to wild type responses. However, CD8+ T cells from A/Japan/57-infected transgenic animals were unable to express cytolytic activity against target cells expressing the A/Japan/57 HA. The CD8+ T cell tolerance was also extremely specific, since transgenics immunized with an influenza strain containing a single amino acid substitution in a dominant HA epitope were able to mount full cytolytic responses to that epitope, but not the wild-type epitope. Adoptive transfer of CD8+ T cell clones into transgenic animals resulted extensive interstitial pneumonitis that was antigen-specific and associated with significant morbidity and mortality. We conclude that a lung-specific transgene may lead to specific CD8+ T cell tolerance, with CD4+ T cell and B cell reactivity to the antigen, and that CD4+ T cell reactivity may remain intact to an antigen expressed in the thymus, even when CD8+ T cell tolerance exists. This observation may have profound implications concerning immune-mediated lung diseases, particularly those mediated by CD4+ T cells.