ABSTRACT
The trend of replacing antimicrobials as growth promoters in animal nutrition is growing. Functional oils emerge as an alternative because of their richness in bioactive compounds and bioavailability. The present study aims to evaluate the fatty acid profile, antioxidant capacity, composition of phenolic compounds, and toxic capacity in Wistar rats of pracaxi oil (Pentaclethra macroloba). DDPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (Ferric Reducing Antioxidant Power), and ABTS (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were performed to assess antioxidant capacity. The composition of phenolic compounds was determined by specific reagents. For the evaluation of subchronic oral toxicity, 40 Wistar albino rats (20 males and 20 females) were randomized into 10 groups with different levels of pracaxi oil administered orally. The doses administered were 0, 300, 600, 1200 and 2400 mg/kg (Group 1 to 5 females and Group 6 to 10 males). The animals were submitted to evaluations described in the OECD manual (Guide 407). The analytical results showed that pracaxi oil has different fatty acids in its chemical composition: oleic, linoleic, arachidic, and behenic acids, which account for more than 90% of its composition. In a smaller percentage, lauric acid (0.17%), myristic (0.09%), palmitic (1.49%), stearic (3.45%), and linolenic acid (1.39%) were also found. According to the results of the antioxidant tests, pracaxi oil has a high antioxidant capacity and is a product with a high presence of phenolic compounds. Regarding the toxicity assessment, there were no alterations in the clinical signs and weight of organs. However, in histology, there were mild alterations of a possible toxic process with the increase in the oil dose. This research is extremely valuable since pracaxi oil is a product with little information about its potential use in animal nutrition.
Subject(s)
Antioxidants , Plant Oils , Animals , Rats , Fatty Acids , Plant Oils/chemistry , Rats, WistarABSTRACT
Prototheca bovis has been associated with several cases of mastitis in cattle but no record of intramammary infections has been reported in goats. This infection does not respond to available treatments and the disposal recommendation of affected animals cause great damage to the dairy industry. Alternatives for dealing with infections caused by Prototheca spp. are required worldwide. In vitro results suggest polypyrrole as promising molecule for combating this alga, because an algaecide effect was observed on tested Prototheca spp. isolates. Thus, this study evaluated goats as an experimental model for intramammary infection by P. bovis and a protocol for treating these animals with an intramammary polypyrrole solution. The possibility of P. bovis promoting an intramammary infection in goats was experimentally proven, demonstrating this species as an important model for studies involving algae mastitis. Furthermore, polypyrrole reduced the counts of Prototheca sp. in the analyzed samples, showing potential to fight this microorganism also in vivo. The results obtained in this study demonstrate the ability of P. bovis to colonize breast tissue in lactating goats and the highly soluble molecule of polypyrrole has potential use for the treatment of protothecosis.
Subject(s)
Mastitis, Bovine , Prototheca , Female , Animals , Cattle , Humans , Polymers/therapeutic use , Lactation , Pyrroles/therapeutic use , Goats , Mastitis, Bovine/drug therapyABSTRACT
A suinocultura industrial compreende o conjunto de produtores que incorporam os avanços tecnológicos em genética, nutrição, sanidade e demais aspectos produtivos, enquanto a suinocultura de subsistência desempenha um papel importante para a alimentação humana, sendo a criação de suínos em criatórios amplamente difundida entre os pequenos proprietários de terra ou em assentamentos. Este trabalho teve como objetivo avaliar a pesquisa de anticorpos contra Brucella sp. nos suínos de granjas comerciais, criatórios de subsistência e javalis asselvajados. Foram visitados 32 criatórios de suínos domésticos localizados na região sul de Mato Grosso do Sul. Considerando-se os fenótipos, verificou-se que os sinais indicativos de contato têm influência do município onde está situada a propriedade (χ2=8.8594, p=0,0029), sendo que o município de Deodápolis tem uma chance de ocorrência mais elevada (OR=13,00;IC95%:2,12-79,59). Através da detecção fenotípicaobserva-sea presença de animais híbridos nas propriedades analisadas, e evidencia-se assim que os animais da propriedade em algum momento entraram em contato com suínos asselvajados, os quais podem ser responsáveis pela disseminação de diversas patologias. A ausência da detecção de anticorpos anti-Brucella sp. em suínos de criatórios sugere que a bactéria não é circulante nas populações de javalis nas áreas de estudo.
Industrial pig farming comprises the group of producers that incorporate technological advances in genetics, nutrition, health and other productive aspects, while subsistence pig farming plays an important role for human nutrition, with the creation of pigs in farms being widely spread among small farms. landowners or settlements. This study aimed to evaluate the search for antibodies against Brucella sp. pigs on commercial farms, livestock farms and wild boars. Were visited 32 domestic swine farms located in the southern region of Mato Grosso do Sul. Considering the phenotypes, it was found that the indicative signs of contact have influence from the municipality where the property is located (χ2=8.8594, p=0.0029) , and the municipality of Deodápolis has a higher chance of occurrence (OR=13,00;IC95%:2,12-79,59). Through the phenotypic detection, the presence of hybrid animals in the analyzed properties is clearly observed, and it is evident that the animals of the property at some point came into contact with pigs, which may be responsible for the spread of several pathogens. The absence of detection of anti-Brucella sp. in breeding pigs it suggests that the bacteria is not circulating in wild boar populations in the study areas.
Subject(s)
Animals , Brucellosis/immunology , Seroepidemiologic Studies , One Health , Swine/microbiology , ZoonosesABSTRACT
A suinocultura industrial compreende o conjunto de produtores que incorporam os avanços tecnológicos em genética, nutrição, sanidade e demais aspectos produtivos, enquanto a suinocultura de subsistência desempenha um papel importante para a alimentação humana, sendo a criação de suínos em criatórios amplamente difundida entre os pequenos proprietários de terra ou em assentamentos. Este trabalho teve como objetivo avaliar a pesquisa de anticorpos contra Brucella sp. nos suínos de granjas comerciais, criatórios de subsistência e javalis asselvajados. Foram visitados 32 criatórios de suínos domésticos localizados na região sul de Mato Grosso do Sul. Considerando-se os fenótipos, verificou-se que os sinais indicativos de contato têm influência do município onde está situada a propriedade (χ2=8.8594, p=0,0029), sendo que o município de Deodápolis tem uma chance de ocorrência mais elevada (OR=13,00;IC95%:2,12-79,59). Através da detecção fenotípicaobserva-sea presença de animais híbridos nas propriedades analisadas, e evidencia-se assim que os animais da propriedade em algum momento entraram em contato com suínos asselvajados, os quais podem ser responsáveis pela disseminação de diversas patologias. A ausência da detecção de anticorpos anti-Brucella sp. em suínos de criatórios sugere que a bactéria não é circulante nas populações de javalis nas áreas de estudo.
Industrial pig farming comprises the group of producers that incorporate technological advances in genetics, nutrition, health and other productive aspects, while subsistence pig farming plays an important role for human nutrition, with the creation of pigs in farms being widely spread among small farms. landowners or settlements. This study aimed to evaluate the search for antibodies against Brucella sp. pigs on commercial farms, livestock farms and wild boars. Were visited 32 domestic swine farms located in the southern region of Mato Grosso do Sul. Considering the phenotypes, it was found that the indicative signs of contact have influence from the municipality where the property is located (χ2=8.8594, p=0.0029) , and the municipality of Deodápolis has a higher chance of occurrence (OR=13,00;IC95%:2,12-79,59). Through the phenotypic detection, the presence of hybrid animals in the analyzed properties is clearly observed, and it is evident that the animals of the property at some point came into contact with pigs, which may be responsible for the spread of several pathogens. The absence of detection of anti-Brucella sp. in breeding pigs it suggests that the bacteria is not circulating in wild boar populations in the study areas.
Subject(s)
Animals , Swine , Brucellosis/veterinary , Seroepidemiologic Studies , Antibodies, BacterialABSTRACT
A leishmaniose é uma zoonose de grande importância para a saúde pública. São grandes os estudos para que se conheçam diferentes técnicas para diagnóstico e que estes testes tenham alta sensibilidade. Este estudo teve como objetivo comparar a sensibilidade do teste parasitológico frente a diferentes locais de colheita de material biológico. As amostras colhidas do linfonodo e medula óssea se mostraram igualmente os mais sensíveis, na ordem de 44% dos casos. Estes valores elevaram-se ao realizar a pesquisa do parasito simultaneamente em duas técnicas.
Leishmaniasis is a zoonosis of great importance to public health. They are great studies to get to know different techniques for diagnosis and these tests have high sensitivity. This study aimed to compare the sensitivity of parasitological testing against different biological material collection sites. Samples taken from the lymph node and bone marrow also showed the most sensitive in the order of 44% of the cases. These figures amounted to perform the search of the parasite simultaneously in both techniques.
La leishmaniosis es una zoonosis de gran importancia para la salud pública. Son grandes los estudios para que se conozcan diferentes técnicas de diagnóstico y que estas pruebas tengan alta sensibilidad. Este estudio tuvo como objetivo comparar la sensibilidad de prueba parasitológica frente a diferentes sitios de recolección de material biológico. Las muestras tomadas de los ganglios linfáticos y médula ósea se demostraron, igualmente, los más sensibles, en el orden de 44% de los casos. Estas cifras se elevaron al realizar la investigación del parásito de forma simultánea en dos técnicas.
