ABSTRACT
A sandwich-type surface-enhanced Raman scattering (SERS) sensor using dual aptamers and gold-enhanced Raman signal probes has been successfully constructed for the detection of tumor-derived extracellular vesicles. The simple and sensitive sensor has the capability to detect tumor extracellular vesicles in 10-fold diluted human serum samples.
Subject(s)
Extracellular Vesicles , Metal Nanoparticles , Neoplasms , Gold , Humans , Neoplasms/diagnosis , Spectrum Analysis, RamanABSTRACT
Herein, we developed a simple and rapid strategy to synthesize gold/silver bimetallic nanoclusters (Au/Ag NCs) with highly biochemical stability by a one-pot route. The Au/Ag NCs were obtained via a chemical reduction procedure in alkaline aqueous solution at 75⯰C within only 20â¯min by employing bovine serum albumin (BSA) as both ligand and reductant. The as-obtained Au/Ag NCs displayed bright orange fluorescence with an emission peak located at 570â¯nm and temperature-dependent fluorescence property, which were utilized as fluorescent thermometer directly. More intriguingly, the Au/Ag NCs were very stable against various pH values, ions, biothiols, H2O2, fetal bovine serum (FBS), RPMI 1640 medium and amino acids. Taking advantage of the excellent biochemical stability, a ratiometric fluorescence biosensor, fluorescein-5-isothiocyanate (FITC)-Au/Ag NCs, was constructed for pH sensing based on the incorporation of FITC into the Au/Ag NCs. Furthermore, the ratiometric pH sensor was also successfully applied on the model of HeLa cells.
Subject(s)
Alloys/chemistry , Gold/chemistry , Metal Nanoparticles/chemistry , Silver/chemistry , Temperature , Alloys/chemical synthesis , Animals , Biosensing Techniques , Cattle , Fluorescein-5-isothiocyanate/chemistry , Fluorescence , HeLa Cells , Humans , Hydrogen-Ion Concentration , Serum Albumin, Bovine/chemistry , Spectrometry, FluorescenceABSTRACT
Exosomes are cell-derived small extracellular vesicles that are naturally secreted by all types of cells and widely distributed in various biofluids. They carry a variety of key bioactive molecules (e.g., nucleic acids, proteins, growth factors, cytokines) from their parent cells and convey them to neighboring or even distant cells through circulation. In recent years, tumor-derived exosomes have attracted great interest from investigators because they actively participate in nearly all aspects of tumor development and facilitate both tumor growth and metastasis through exosome-mediated intercellular communication. The vesicular contents are increasingly considered potential biomarkers for tumor diagnoses and prognosis. With the progress made in isolation and analytical technologies, the functions of exosomes and their contents in tumor development are also becoming clearer. In this review article we describe the recent developments in exosome isolation techniques and analysis of exosomal contents. We also address their applications in cancer detection and therapy.
Subject(s)
Exosomes/metabolism , Neoplasms/diagnosis , Antineoplastic Agents/chemistry , Antineoplastic Agents/therapeutic use , Biomarkers, Tumor/genetics , Biomarkers, Tumor/metabolism , Cell Communication , Drug Carriers/chemistry , Exosomes/chemistry , Humans , MicroRNAs/metabolism , Neoplasms/drug therapy , Neoplasms/pathology , Proteins/metabolism , RNA, Messenger/metabolismABSTRACT
An enzyme-free and ultrasensitive fluorescence assay for the detection of nucleic acids was successfully established by a hybridization-triggered DNAzyme cascade (HTDC). This simple and cost-effective sensor has good sensitivity, selectivity and the capability for detection of target DNA from complex fluids.
Subject(s)
Biological Assay/methods , DNA, Catalytic/chemistry , DNA/analysis , Nucleic Acid Amplification Techniques/methods , Base Sequence , DNA/chemistry , Fluorescence , Fluorescent Dyes/chemistry , Nucleic Acid Hybridization , Zinc/chemistryABSTRACT
A novel and adjustable lamp based on competitive interaction among dsDNA-SYBR Green I (SGI), ion quencher, and analyte was designed for bioanalysis. The "filament" and switch of the lamp could be customized by employing different dsDNA and ion quencher. The poly(AT/TA) dsDNA was successfully screened as the most effective filament of the lamp. Two common ions, Hg2+ and Fe3+, were selected as the model switch, and the corresponding ligand molecules cysteine (Cys) and pyrophosphate ions (PPi) were selected as the targets. When the fluorescence-quenched dsDNA/SGI-ion complex was introduced into a target-containing system, ions could be bound by competitive molecules and separate from the complex, thereby lighting the lamp. However, no light was observed if the biomolecule could not snatch the metal ions from the complex. Under the optimal conditions, sensitive and selective detection of Cys and PPi was achieved by the lamp, with practical applications in fetal bovine serum and human urine. This ion quencher regulated lamp for fluorescent bioassays is simple in design, fast in operation, and is more convenient than other methods. Significantly, as many molecules could form stable complexes with metal ions selectively, this ion quencher operated lamp has potential for the detection of a wide spectrum of analytes. Graphical abstract A novel and adjustable lamp on the basis of competitive interaction among dsDNA-SYBR Green I, ions quencher and analyte was designed for bioanalysis. The filament and switch of lamp could be customized by employing different dsDNA and ions quencher.