ABSTRACT
Climate change is dramatically altering global precipitation patterns across terrestrial ecosystems, making it critically important that we understand both how and why plant species vary in their drought sensitivities. Andropogon gerardii and Schizachyrium scoparium, both C4 grasses, provide a model system for understanding the physiological mechanisms that determine how species of a single functional type can differ in drought responses, an issue remains a critical gap in our ability to model and predict the impacts of drought on grassland ecosystems. Despite its greater lability of foliar water content, previous experiments have demonstrated that S. scoparium maintains higher photosynthetic capacity during droughts. It is therefore likely that the ability of S. scoparium to withstand drought instead derives from a greater metabolic resistance to drought. Here, we tested the following hypotheses: (H1) A. gerardii is more vulnerable to drought than S. scoparium at both the population and organismal levels, (H2) A. gerardii is less stomatally flexible than S. scoparium, and (H3) A. gerardii is more metabolically limited than S. scoparium. Our results indicate that it is actually stomatal limitations of CO2 supply that limit A. gerardii photosynthesis during drought. Schizachyrium scoparium was more drought-resistant than A. gerardii based on long-term field data, organismal biomass production and physiological gas exchange measurements. While both S. scoparium and A. gerardii avoided metabolic limitation of photosynthesis, CO2 supply of A. gerardii was greatly reduced during late-stage drought stress. That two common, co-occurring C4 species possess such different responses to drought highlights the physiological variability inherent within plant functional groups and underscores the need for more studies of C4 drought tolerance.
ABSTRACT
Over 180 non-native species have been introduced in the Laurentian Great Lakes region, many posing threats to native species and ecosystem functioning. One potential pathway for introductions is the commercial bait trade; unknowing or unconcerned anglers commonly release unused bait into aquatic systems. Previous surveillance efforts of this pathway relied on visual inspection of bait stocks in retail shops, which can be time and cost prohibitive and requires a trained individual that can rapidly and accurately identify cryptic species. Environmental DNA (eDNA) surveillance, a molecular tool that has been used for surveillance in aquatic environments, can be used to efficiently detect species at low abundances. We collected and analyzed 576 eDNA samples from 525 retail bait shops throughout the Laurentian Great Lake states. We used eDNA techniques to screen samples for multiple aquatic invasive species (AIS) that could be transported in the bait trade, including bighead (Hypophthalmichthys nobilis) and silver carp (H. molitrix), round goby (Neogobius melanostomus), tubenose goby (Proterorhinus marmoratus), Eurasian rudd (Scardinius erythrophthalmus), and goldfish (Carassius auratus). Twenty-seven samples were positive for at least one target species (4.7% of samples), and all target species were found at least once, except bighead carp. Despite current regulations, the bait trade remains a potential pathway for invasive species introductions in the Great Lakes region. Alterations to existing management strategies regarding the collection, transportation, and use of live bait are warranted, including new and updated regulations, to prevent future introductions of invasive species in the Great Lakes via the bait trade.
