ABSTRACT
The characteristics of a unique genetic collection of radish inbred lines and the history of its creation and study are presented. The possible research prospects are discussed.
Subject(s)
Inbreeding , Raphanus/classification , Raphanus/genetics , History, 20th Century , History, 21st Century , RussiaABSTRACT
The results of genetic analysis of the wavy shoots character supported the hypothesis on the existence of two genes, Wsh1 and Wsh2, whose mutations caused wavy shoots in radish lines 16 and 26, respectively. As shown by analysis of joint inheritance with marker characters, wavy shoots were inherited independently of the biochemical markers (genes Lap1, Ep, Dia1, and Aat). Gene Wsh1 was shown to be linked to genes A, ac, and ar; the mode of inheritance depended on the cross combination. In hybrids with mutant line 16, plants with novel morphological defects, which were absent in the parental forms, were detected. Possible mechanisms of new morphological abnormalities and change of linkage in the combinations analyzed are discussed.
Subject(s)
Genes, Plant/genetics , Mutation , Plant Shoots/genetics , Quantitative Trait, Heritable , Raphanus/genetics , Genetic Markers/geneticsABSTRACT
We characterized radish lines from a genetic collection on the basis of six enzyme systems, identified genes controlling these enzymes, and examined joint inheritance of some biochemical and morphological traits.
Subject(s)
Enzymes/metabolism , Raphanus/enzymology , Aconitate Hydratase/genetics , Aconitate Hydratase/metabolism , Aspartate Aminotransferases/genetics , Aspartate Aminotransferases/metabolism , Endopeptidases/genetics , Endopeptidases/metabolism , Enzymes/genetics , Genetic Markers , Hybridization, Genetic , Leucyl Aminopeptidase/genetics , Leucyl Aminopeptidase/metabolismABSTRACT
Tumor formation was studied in inbred radish lines that produce tumors on plant roots during flowering. In all radish lines under consideration, the sequences homologous to oncogenes tmr/tml of Agrobacterium tumefaciens were revealed by Southern hybridization. No sequences homologous to the tms locus of A. tumefaciens and the oncogenes of A. rhizogenes were determined. It was found that auxin sensitivity and the tumor-producing capacity were coinherited. We suggest that tumor phenotype arise as a result of a combination between agrobacterial "cytokinin" oncogenes and certain alleles of "auxin" radish genes.
