ABSTRACT
In the title compound, C(9)H(9)FO(3), the dihedral angle between the carboxyl group and the benzene ring is 79.4â (3)°. In the crystal, mol-ecules form centrosymmetric dimers through pairs of classical O-Hâ¯O hydrogen bonds. These are further linked by weaker C-Hâ¯O inter-actions, forming a three-dimensional network.
ABSTRACT
The title mol-ecule, C(13)H(18)N(2)O(3), contains a benzene ring fused to an oxazine ring and one tert-but-oxy-carbonyl group bound to the N atom of the oxazine ring. A weak intra-molecular C-Hâ¯O inter-action occurs. In the crystal, inter-molecular N-Hâ¯O and C-Hâ¯O hydrogen bonds stack the mol-ecules down the b axis. Weak C-Hâ¯N contacts connect the stacks, generating a three-dimensional network.
ABSTRACT
AIM: To study the inhibitory effect of 23-HBA on angiogenesis in vitro. METHODS: The effect of 23-hydroxy butulinic acid (23-HBA) on the in vitro proliferation of human microcapillary endothelial cells(HMECs) was examined by sulfonylrhodamine B (SRB) assay. The effect of 23-HBA on endothelial cell migration, and tubule formation on Matrigel was also observed. The CD31 expression in HMECs was dectected by immunohistochemical staining. RESULTS: The proliferation of HMECs was inhibited significantly by 23-HBA with IC(50) being 40.44 mg/L. 23-HBA inhibited endothelial cell migration and tubule formation in a dose-dependent manner. The expression of CD31 in HMECs was reduced after treatment with 10 mg/L 23-HBA. CONCLUSION: 23-HBA can inhibit angiogenesis in vitro, which would become a promising antiangiogenic drug.
