ABSTRACT
Urate crystal size change and the modification of coated proteins by oxygen radicals released by stimulated polymorphonuclear cells (PMN) could represent a likely "switch-off" mechanism of the spontaneous resolution of acute gout attack. The absorption spectra and the uric acid, allantoin and urea concentrations were determined before and after in vitro exposition of monosodium urate (MSU) crystals to superoxide anion (O2) photochemically generated. The results showed a complete dissolution of MSU crystals after incubation under O2-, with decrease of uric acid and increase of allantoin and urea concentrations. Our results were confirmed by polarizing, electron microscopy and calorimetric techniques. The results obtained seem to confirm that the spontaneous resolution of acute gout attack could be attributed to the dissolving effect on urate crystals and the modification of coated proteins by O2- released by phagocytizing PMN.
Subject(s)
Acute-Phase Reaction/physiopathology , Gout/physiopathology , Inflammation/physiopathology , Calorimetry , Crystallization , Humans , Lymphocytes/metabolism , Microscopy, Electron, Scanning , Remission, Spontaneous , Superoxides/metabolism , Superoxides/pharmacology , Uric Acid/metabolismSubject(s)
Ferric Compounds/pharmacology , Ferrous Compounds/pharmacology , Iron/pharmacology , Neutrophils/drug effects , Oxygen/metabolism , Arthritis, Rheumatoid/pathology , Deferoxamine/pharmacology , Humans , Hydroxides , Hydroxyl Radical , In Vitro Techniques , Luminescent Measurements , Zymosan/pharmacologySubject(s)
Blood Proteins/metabolism , Estradiol/pharmacology , Progesterone/pharmacology , Testosterone/pharmacology , Uric Acid/blood , Colorimetry , Contraceptives, Oral , Cyclofenil/therapeutic use , Female , Humans , In Vitro Techniques , Norethindrone/analogs & derivatives , Norethindrone/therapeutic use , Norethindrone Acetate , Protein Binding , Time Factors , UltrafiltrationSubject(s)
Anti-Inflammatory Agents/pharmacology , Neutrophils/metabolism , Oxygen/metabolism , Arthritis, Rheumatoid/pathology , Aspirin/pharmacology , Cell Membrane/drug effects , Diclofenac/pharmacology , Free Radicals , Humans , Indomethacin/pharmacology , Luminescent Measurements , Neutrophils/drug effects , Piroxicam , Thiazines/pharmacology , Zymosan/pharmacologyABSTRACT
The Authors attested the evidence of a cross-reactivity among some Klebsiella antigens and HLA B 27 positive lymphocytes, suggesting the hypothesis that Klebsiella may be an initiating agent in ankylosing spondylitis (AS). The Authors determined also serum antibody response against Klebsiella antigens in AS patients. The results obtained are discussed.
