ABSTRACT
In August 2020, anew West Nile virus (WNV) outbreak affected 71 people with meningoencephalitis in Andalusia (Spain). Samples from these individuals were received in our laboratory, a regional Virus Referral Centre. The aim of this study was to compare the agreement, sensitivity and specificity of findings between the WNV VIRCLIA IgG and IgM assay (Vircell, Spain) and the WNV ELISA IgM and IgG assay (Euroimmun, Germany) and to compare the performance of WNV VIRCLIA IgM and Euroimmun ELISA for cerebrospinal fluid (CSF) diagnosis. The study included 24 CSF samples (paired with serum samples) and 247 serum samples from 217 patients with suspected WNV infection (1 or 2 per patient). The agreement between ELISA and CLIA tests for IgM and Ig G detection in serum was 93% (kappa index = 0.85) and 96% (kappa index = 0.89) respectively. Sensitivity values of ELISA and CLIA tests for IgM in serum samples were 96.7% and 98.9%, respectively, and specificity values were 96.4% and 95.4% respectively. Sensitivity values of ELISA and CLIA test for IgG in serum samples were 91.1% and 97%, respectively, and specificity values were 100% and 98.8% respectively. Results obtained with ELISA and CLIA tests in CSF samples showed 75% agreement between them (kappa index = 0.51). According to these findings, the WNV VIRCLIA IgM and IgG monotest offers an accurate qualitative detection of WNV in serum and CSF specimens.
Subject(s)
West Nile Fever , West Nile virus , Antibodies, Viral , Enzyme-Linked Immunosorbent Assay/methods , Humans , Immunoglobulin M , West Nile Fever/diagnosis , West Nile Fever/epidemiologyABSTRACT
The objectives of this study were to report on the antimicrobial susceptibility of 141 clinically significant anaerobic bacteria isolated from bloodstream infections between January 2016 and April 2020 in a tertiary-care hospital in Granada (Spain) and to describe the main clinical features of the patients. Species identification was performed by MALDI-TOF MS (Bruker Daltonics, Billerica, MA, USA). Antimicrobial susceptibility tests were performed against penicillin, amoxicillin-clavulanic acid, imipenem, moxifloxacin, clindamycin, metronidazole, and piperacillin-tazobactam using the gradient diffusion technique and EUCAST breakpoints, except for moxifloxacin (CLSI breakpoints). The most frequent anaerobes were Bacteroides (43.9%, n = 62), Clostridium (24.1%, n = 34) and Gram-positive anaerobic cocci (GPACs) (15.6%, n = 22). Almost all tested anaerobes were susceptible to imipenem and amoxicillin-clavulanic acid, except for Bacteroides. High overall resistance rates to clindamycin were observed, especially for Gram-positive anaerobic cocci (GPACs) (54.5%) and for Bacteroides spp. (45.1%). Overall, low resistance rates to almost all antibiotics were observed for Clostridium. High resistance rates to penicillin were also observed for Gram-positive anaerobic bacilli (GPABs) (44.4%), as well as to metronidazole (22.2%), although only nine isolates were included. Antimicrobial susceptibility testing for anaerobes should always be performed in severe infections, such as those localized in the bloodstream. The information obtained contributes to selecting empirical treatments according with local data on resistance.
ABSTRACT
No disponible
Subject(s)
Humans , Female , Middle Aged , Bone Diseases, Infectious/microbiology , Flavobacteriaceae/isolation & purification , Flavobacteriaceae Infections/microbiology , Foot Injuries/microbiology , Anti-Bacterial Agents/therapeutic use , Bone Diseases, Infectious/drug therapy , Flavobacteriaceae/drug effects , Flavobacteriaceae Infections/drug therapy , Foot Injuries/drug therapyABSTRACT
Finegoldia magna is a Gram-positive anaerobic cocci frequently reported in human diseases. We report a rare case of mechanical prosthetic endocarditis due to this microorganism in a patient with heart disease. A 50-year-old man with prosthetic mitral and aortic valve presented with pericardial effusion, cardiac tamponade, and multiorgan dysfunction. Anaerobic blood cultures yielded a positive result, allowing further identification as F magna by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The patient suffered replacement of mechanical mitral prosthesis by a new mechanical prosthesis, growing also F magna in the valvular culture. The isolate was identified as F magna by 16S ribosomal RNA sequence analysis. As a complication, a convulsive episode occurred, but a positive outcome was finally observed.
ABSTRACT
Eggerthia catenaformis is a Gram-positive anaerobic rod, which has been rarely reported in human diseases. We report the second case of bacteremia due to this microorganism in an elderly patient. A 73-year-old man, without underlying diseases presented with fever, odynophagia and swelling of the cervical lymph node for several days. Culture of drained cervical fluid resulted in the isolation of Raoultella ornithinolytica and Streptococcus anginosus. Anaerobic blood cultures yielded a rare anaerobic microorganism, identified as Eggerthia catenaformis. No resistance to tested antimicrobials was documented. Treatment with drainage and several antibiotic regimens was established, and the general condition of the patient improved, at two months of follow-up.
Subject(s)
Abscess/complications , Bacteremia/diagnosis , Bacteremia/pathology , Firmicutes/isolation & purification , Gram-Positive Bacterial Infections/diagnosis , Gram-Positive Bacterial Infections/pathology , Periodontal Diseases/complications , Abscess/diagnosis , Aged , Anti-Bacterial Agents/therapeutic use , Blood/microbiology , Drainage , Enterobacteriaceae/isolation & purification , Enterobacteriaceae Infections/diagnosis , Enterobacteriaceae Infections/microbiology , Humans , Lymphadenitis/complications , Lymphadenitis/diagnosis , Lymphadenitis/microbiology , Lymphadenitis/therapy , Male , Periodontal Diseases/diagnosis , Streptococcal Infections/diagnosis , Streptococcal Infections/microbiology , Streptococcus anginosus/isolation & purificationABSTRACT
No disponible
Subject(s)
Humans , Female , Adult , Breast Diseases/microbiology , Gram-Positive Rods/isolation & purification , Abscess/microbiology , Gram-Positive Rods/pathogenicity , Coinfection/microbiologyABSTRACT
Trueperella bernardiae es un microorganismo grampositivo facultativo anaerobio que forma parte de la microbiota normal de la piel y de la orofaringe, que recientemente ha sido reasignado l género Trueperella. Este patógeno ha sido descrito en muy pocos casos como causante de infección en los seres humanos, debido a su aspecto corineforme y su presencia en cultivos mixtos, y a las dificultades diagnósticas
Subject(s)
Humans , Actinomycetaceae , Abscess , Bacterial Infections , Gram-Positive BacteriaABSTRACT
Prevotella dentalis is a Gram-negative anaerobic rod involved in various human diseases, especially oral infections. We report a rare case of a pleural effusion due to this microorganism in an elderly patient. An 88-year-old man with chronic respiratory disease presented with a left pleural effusion for more than 1 month. Culture of drained pleural fluid resulted in isolation of P. dentalis. Resistance to penicillin and moxifloxacin was documented. Treatment with drainage and clindamycin was established, but the patient developed cognitive impairment and died after a worsening of his general condition.
