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1.
An Acad Bras Cienc ; 90(3): 2955-2965, 2018.
Article in English | MEDLINE | ID: mdl-30304227

ABSTRACT

This work aimed to evaluate the production of lipolytic complexes, produced by microorganisms isolated from a biological treatment system of effluents from a hotel. To select the best lipolytic microorganism for use in biotechnological processes, we tested 45 bacterial isolates recovered from the raw effluent of the hotel's restaurant waste tank. Lipase production was assayed in culture medium supplemented with olive oil and rhodamine B, incubated at 25 °C and 30 °C for 24 h - 48 h. Results showed 22 isolates lipase producers. All isolates were inoculated on medium without yeast extract to select the ones with highest enzyme yields. Out of these, nine isolates showed high lipase activity. The strain with the larger halo was assayed in submerged culture using an orbital shaker and a bioreactor, with three different substrates (olive oil, grape seed oil, and canola oil). Isolate G40 identified as Acinetobacter baylyi was selected to run the production assays because it showed the best result in the solid medium. In the bioreactor, maximum lipase production was obtained after 12 h of culture with the three substrates evaluated: 0,358 U/mL.min-1 in olive oil, 0,352 U/mL.min-1 with grapeseed oil, and 0,348 U/mL.min-1 with canola oil.


Subject(s)
Bacteria/enzymology , Industrial Waste , Lipolysis/physiology , Wastewater/microbiology , Bacteria/isolation & purification , Bacterial Physiological Phenomena , Culture Media/pharmacology , Olive Oil/pharmacology , Rapeseed Oil/pharmacology , Temperature , Time Factors , Waste Disposal, Fluid , Wastewater/analysis
2.
Appl Opt ; 48(26): 4880-4, 2009 Sep 10.
Article in English | MEDLINE | ID: mdl-19745848

ABSTRACT

We study imaging systems designed to assess the smile of laser diode bars (LDBs). The magnification matrix is derived from the required sampling period and the geometries of the LDBs and the charge-coupled device (CCD) array. These image-forming systems present in-plane pure translation invariance, but in the case of anamorphic ones, lack in-plane rotation invariance. It is shown that the smile parameters of the image of the LDB are linked with the smile parameters of the LDB by simple mathematical expressions. The spatial resolution of such optical systems is estimated at approximately 1 microm for a mean wavelength of lambda approximately 800 nm. Our results suggest that, with the current state-of-the-art, the formation of imaging methods for LDB smile assessment can be used to assess smile heights > or = 1 microm.

3.
J. pediatr. (Rio J.) ; J. pediatr. (Rio J.);75(supl.2): S251-S258, dez. 1999. tab
Article in Portuguese | LILACS | ID: lil-256369

ABSTRACT

Objetivo: O artigo tem por finalidade reafirmar a importância dos animais peçonhentos na infância. As condutas preconizadas são baseadas naquelas propostas pelo Ministério da Saúde do Brasil para padronização do atendimento nesse tipo de acidente. Procura mostrar a importância do diagnóstico clínico e do atendimento precoce. Método: Foi realizada revisão bibliográfica da literatura internacional e nacional, aaabrangendo artigos originais, normas oficiais e capítulos de livros. Resultados: O atendimento por picadas de animais peçonhentos traz ansiedade e insegurança para o pediatra que nem sempre está habituado com essa patologia. O presente artigo procura oferecer um roteiro de fácil compreensão e aplicação nesses atendimentos. AS açoes mais importantes e as principais a serem realizadas estão aqui descritas. Particularidades pouco freqüentes e às vezes inusitadas, com certeza, poderão ser encontradas na bibliografia oferecida no final do trabalho. O acidente por animal peçonhento sempre é mais grave na criança, conseqüentemente as seqüelas e a mortalidade são mais altas. Afirmamos que o atendimento precoce através da soroterapia é de grande importância. Medidas folclóricas e mesmo empíricas devem ser abolidas. Conclusões: A sistematização do atendimento, para evitar que pontos importantes não sejam esquecidos e medidas de pequena importância sejam realizadas, além de facilitar, tornam a assistência e mais ágil efetiva. Os roteiros apresentados no artigo têm essa finalidade


Subject(s)
Humans , Child , Accidents , Anaphylaxis , Antivenins/therapeutic use , Immunization, Passive , Necrosis , Poisons , Venoms , Elapid Venoms , Hymenoptera , Scorpion Venoms , Snake Venoms , Spider Venoms
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