Enhancer-AAVs allow genetic access to oligodendrocytes and diverse populations of astrocytes across species.

Proper brain function requires the assembly and function of diverse populations of neurons and glia. Single cell gene expression studies have mostly focused on characterization of neuronal cell diversity; however, recent studies have revealed substantial diversity of glial cells, particularly astrocytes. To better understand glial cell types and their roles in neurobiology, we built a new suite of adeno-associated viral (AAV)-based genetic tools to enable genetic access to astrocytes and oligodendrocytes. These oligodendrocyte and astrocyte enhancer-AAVs are highly specific (usually > 95% cell type specificity) with variable expression levels, and our astrocyte enhancer-AAVs show multiple distinct expression patterns reflecting the spatial distribution of astrocyte cell types. To provide the best glial-specific functional tools, several enhancer-AAVs were: optimized for higher expression levels, shown to be functional and specific in rat and macaque, shown to maintain specific activity in epilepsy where traditional promoters changed activity, and used to drive functional transgenes in astrocytes including Cre recombinase and acetylcholine-responsive sensor iAChSnFR. The astrocyte-specific iAChSnFR revealed a clear reward-dependent acetylcholine response in astrocytes of the nucleus accumbens during reinforcement learning. Together, this collection of glial enhancer-AAVs will enable characterization of astrocyte and oligodendrocyte populations and their roles across species, disease states, and behavioral epochs.

Neural Plasticity in Sensorimotor Brain-Machine Interfaces.

Brain-machine interfaces (BMIs) aim to treat sensorimotor neurological disorders by creating artificial motor and/or sensory pathways. Introducing artificial pathways creates new relationships between sensory input and motor output, which the brain must learn to gain dexterous control. This review highlights the role of learning in BMIs to restore movement and sensation, and discusses how BMI design may influence neural plasticity and performance. The close integration of plasticity in sensory and motor function influences the design of both artificial pathways and will be an essential consideration for bidirectional devices that restore both sensory and motor function.

Windows and periscopes into primate behavior.

Calcium imaging of neurons in monkeys making reaches is complicated by brain movements and limited by shallow imaging depth. In a pair of recent studies, Trautmann et al., 2021 and Bollimunta et al. (2021) present complementary solutions to these problems.

Distance and Angle Correction System (DACS) for a kHz A-Scan Rate Pump-Probe Laser-Ultrasound Inspection.

Non-contact optical detection of ultrasound critically depends on the amount of light collected from the detection surface. Although it can be optimized in multiple ways for an ideal flat polished surface, industrial non-destructive testing and evaluation (NDT&E) usually requires optical detectors to be robust for unpolished material surfaces that are usually rough and curved. Confocal detectors provide the best light collection but must trade off sensitivity with depth of field. Specifically, detection efficiency increases with the numerical aperture (NA) of the detector, but the depth of field drops. Therefore, fast realignment of the detector focal point is critical for in-field applications. Here, we propose an optical distance and angle correction system (DACS) and demonstrate it in a kHz-rate laser-ultrasound inspection system. It incorporates a Sagnac interferometer on receive for the fast scanning of aircraft composites, which minimizes the required initial alignment. We show that DACS performs stably for different composite surfaces while providing ±2° angular and ±2 mm axial automatic correction with a maximum 100 ms realignment time.