Abnormal energy metabolism in the pathogenesis of systemic lupus erythematosus.

Systemic lupus erythematosus (SLE) is a severe autoimmune disease with significant socioeconomic impact worldwide. Orderly energy metabolism is essential for normal immune function, and disordered energy metabolism is increasingly recognized as an important contributor to the pathogenesis of SLE. Disorders of energy metabolism are characterized by increased reactive oxygen species, ATP deficiency, and abnormal metabolic pathways. Oxygen and mitochondria are critical for the production of ATP, and both mitochondrial dysfunction and hypoxia affect the energy production processes. In addition, several signaling pathways, including mammalian target of rapamycin (mTOR)/adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) signaling and the hypoxia-inducible factor (HIF) pathway also play important regulatory roles in energy metabolism. Furthermore, drugs with clear clinical effects on SLE, such as sirolimus, metformin, and tacrolimus, have been proven to improve the disordered energy metabolism of immune cells, suggesting the potential of targeting energy metabolism for the treatment of SLE. Moreover, several metabolic modulators under investigation are expected to have potential therapeutic effects in SLE. This review aimed to gain insights into the role and mechanism of abnormal energy metabolism in the pathogenesis of SLE, and summarizes the progression of metabolic modulator in the treatment of SLE.

Gastric point-of-care ultrasonography in patients undergoing radical gastrointestinal surgery before anesthetic induction: an observational cohort study.

BACKGROUND: Pulmonary aspiration of gastric contents is a serious perioperative complication. Patients with gastric cancer may experience delayed gastric emptying. However, the role of qualitative and quantitative gastric ultrasound assessments in this patient population before anesthesia induction has not yet been determined. METHODS: Adult patients with gastrointestinal cancer were recruited and examined using gastric point-of-care ultrasound (POCUS) before anesthetic induction from March 2023 to August 2023 in a tertiary cancer center. Three hundred patients with gastric cancer were conducted with POCUS prior to induction, and three hundred patients with colorectal cancer were included as controls. The cross-sectional area (CSA) of the gastric antrum and gastric volumes (GV) were measured and calculated. We determined the nature of the gastric contents and classified the antrum using a 3-point grading system. A ratio of GV to body weight > 1.5mL/Kg was defined as a high risk of aspiration. RESULTS: In patients with gastric cancer, 70 patients were classified as grade 2 (23%, including 6 patients with solid gastric contents) and 63 patients (21%) were identified as having a high risk of aspiration. Whereas in patients with colorectal cancer, only 11 patients were classified as grade 2 (3.7%), and 27 patients (9.7%) were identified as having a high risk of aspiration. A larger tumor size (OR:1.169, 95% CI 1.045-1.307, P = 0.006), tumor located in antrum (OR:2.304, 95% CI 1.169-4.539,P = 0.016), gastrointestinal obstruction (OR:21.633, 95% CI 4.199-111.443, P < 0.0001) and more lymph node metastasis (OR:2.261, 95% CI 1.062-4.812, P = 0.034) were found to be positively while tumor site at cardia (OR:0.096, 95% CI 0.019-0.464, P = 0.004) was negatively associated with high aspiration risk in patients with gastric cancer. CONCLUSION: The Gastric POCUS prior to induction provides an assessment of the status of gastric emptying and can identify the patients at high risk of aspiration, especially those with gastric cancer. TRIAL REGISTRATION: Chinese Clinical Trial Registry ( www.chictr.org.cn ) identifier: ChiCTR2300069242; registered 10 March 2023.

Changes in information integration and brain networks during propofol-, dexmedetomidine-, and ketamine-induced unresponsiveness.

BACKGROUND: Information integration and network science are important theories for quantifying consciousness. However, whether these theories propose drug- or conscious state-related changes in EEG during anaesthesia-induced unresponsiveness remains unknown. METHODS: A total of 72 participants were randomised to receive i.v. infusion of propofol, dexmedetomidine, or ketamine at a constant infusion rate until loss of responsiveness. High-density EEG was recorded during the consciousness transition from the eye-closed baseline to the unresponsiveness state and then to the recovery of the responsiveness state. Permutation cross mutual information (PCMI) and PCMI-based brain networks in broadband (0.1-45 Hz) and sub-band frequencies were used to analyse drug- and state-related EEG signature changes. RESULTS: PCMI and brain networks exhibited state-related changes in certain brain regions and frequency bands. The within-area PCMI of the frontal, parietal, and occipital regions, and the between-area PCMI of the parietal-occipital region (median [inter-quartile ranges]), baseline vs unresponsive were as follows: 0.54 (0.46-0.58) vs 0.46 (0.40-0.50), 0.58 (0.52-0.60) vs 0.48 (0.44-0.53), 0.54 (0.49-0.59) vs 0.47 (0.42-0.52) decreased during anaesthesia for three drugs (P<0.05). Alpha PCMI in the frontal region, and gamma PCMI in the posterior area significantly decreased in the unresponsive state (P<0.05). The frontal, parietal, and occipital nodal clustering coefficients and parietal nodal efficiency decreased in the unresponsive state (P<0.05). The increased normalised path length in delta, theta, and gamma bands indicated impaired global integration (P<0.05). CONCLUSIONS: The three anaesthetics caused changes in information integration patterns and network functions. Thus, it is possible to build a quantifying framework for anaesthesia-induced conscious state changes on the EEG scale using PCMI and network science.

Facile solvothermal preparation of an organic hybrid dysprosium selenidoantimonate for an efficient oxygen evolution reaction.

To overcome the issue of the sluggish kinetics in the oxygen evolution reaction (OER), the development of an efficient OER electrocatalyst with high intrinsic activity is very desirable for green hydrogen energy utilization from electrochemical water splitting. Herein, a facile and feasible solvothermal reaction of Sb, Se, DyCl3 and triethylenetetramine (teta) at 170 °C for 7 days achieved a new organic hybrid dysprosium selenidoantimonate [Dy(teta)2][SbSe4] (SbSe-1), which comprises discrete [SbSe4]3- and [Dy(teta)2]3+ ions. SbSe-1 was utilized in combination with acetylene black (AB), Ni nanoparticles and the porous Ni foam (NF) support to fabricate a Ni/SbSe-1@AB/NF electrode as an efficient anodic electrocatalyst, showing excellent OER electrocatalytic performance with a low overpotential of 269 mV at 10 mA cm-2. Although some antimony chalcogenides are used as electrocatalysts for the water splitting, organic hybrid lanthanide chalcogenidoantimonates applied as OER electrocatalysts have not emerged. Therefore, SbSe-1 offers the first example of an organic hybrid lanthanide chalcogenido-antimonate as an OER electrocatalyst.

Altered brain dynamics index levels of arousal in complete locked-in syndrome.

Complete locked-in syndrome (CLIS) resulting from late-stage amyotrophic lateral sclerosis (ALS) is characterised by loss of motor function and eye movements. The absence of behavioural indicators of consciousness makes the search for neuronal correlates as possible biomarkers clinically and ethically urgent. EEG-based measures of brain dynamics such as power-law exponent (PLE) and Lempel-Ziv complexity (LZC) have been shown to have explanatory power for consciousness and may provide such neuronal indices for patients with CLIS. Here, we validated PLE and LZC (calculated in a dynamic way) as benchmarks of a wide range of arousal states across different reference states of consciousness (e.g., awake, sleep stages, ketamine, sevoflurane). We show a tendency toward high PLE and low LZC, with high intra-subject fluctuations and inter-subject variability in a cohort of CLIS patients with values graded along different arousal states as in our reference data sets. In conclusion, changes in brain dynamics indicate altered arousal in CLIS. Specifically, PLE and LZC are potentially relevant biomarkers to identify or diagnose the arousal level in CLIS and to determine the optimal time point for treatment, including communication attempts.

Deep-Red Luminescent Cuprous-Lead Bromide as a Dual-Responsive Sensor for Fe3+ and Cr2O72.

Both optically active 1-tetrazole-4-imidazole-benzene (TIB) with bifunctional azole groups and heterometals were utilized to build a new type of one-dimensional (1-D) hybrid cuprous-lead bromide [PbCu2Br4(TIB)2]n (1), which exhibits infrequent deep-red luminescent emission at 704 nm with a large Stokes shift of 321 nm. Owing to the existence of rare free Lewis basic imidazole groups, 1 can be used as the sole dual-responsive luminescent sensor for the efficient and selective detection of Fe3+ and Cr2O72- in an aqueous solution.

Resting State Functional Connectivity Analysis During General Anesthesia: A High-Density EEG Study.

The depth of anesthesia monitoring is helpful to guide administrations of general anesthetics during surgical procedures,however, the conventional 2-4 channels electroencephalogram (EEG) derived monitors have their limitations in monitoring conscious states due to low spatial resolution and suboptimal algorithm. In this study, 256-channel high-density EEG signals in 24 subjects receiving three types of general anesthetics (propofol, sevoflurane and ketamine) respectively were recorded both before and after anesthesia. The raw EEG signals were preprocessed by EEGLAB 14.0. Functional connectivity (FC) analysis by traditional coherence analysis (CA) method and a novel sparse representation (SR) method. And the network parameters, average clustering coefficient (ACC) and average shortest path length (ASPL) before and after anesthesia were calculated. The results show SR method find more significant FC differences in frontal and occipital cortices, and whole brain network (p<0.05). In contrast, CA can hardly obtain consistent ASPL in the whole brain network (p>0.05). Further, ASPL calculated by SR for whole brain connections in all of three anesthesia groups increased, which can be a unified EEG biomarker of general anesthetics-induced loss of consciousness (LOC). Therefore FC analysis based on SR analysis has better performance in distinguishing anesthetic-induced LOC from awake state.

Hirsutanol A exhibits neuroprotective activities against sevoflurane-induced neurotoxicity in aged rats.

The neurotoxicity of the inhaled anesthetic, sevoflurane, has been documented in a number of studies. In this study, we conducted experiments to investigate whether Hirsutanol A (HA), a sesquiterpene compound from the fungus, Chondrostereum sp., can provide protection from sevoflurane-induced neurological toxicity in aged rats, and analyzed the underlying mechanisms. The cognitive dysfunction of rats following sevoflurane exposure was evaluated by behavioral tests. The neuronal cell survival was determined by Nissl staining. In addition, human neuroblastoma H4 cells were exposed to sevoflurane to establish an in vitro model. Apoptotic marker expression in hippocampal tissue was determined by western blotting. Cell apoptosis in vitro was also examined by TUNEL assay and flow cytometry. The expression and translocation of Nrf2 were examined by both western blot and immunofluorescence staining. Our results show that HA significantly attenuated sevoflurane-induced cognitive impairment in aged rats. In addition, HA treatment decreased sevoflurane-induced neuronal apoptosis in the hippocampus and alleviated Aß accumulation. Our results also show that the neuroprotective effect of HA is associated with the activation of Nrf2 signaling. Human neuroblastoma H4 cells were used as a model to examine the protective activity of HA against sevoflurane-induced neurotoxicity. In addition, our results show that the inhibition of Nrf2 by a specific inhibitor or targeting siRNA significantly compromises the attenuating effect of HA on sevoflurane-induced cell apoptosis and Aß accumulation. Our results suggest that HA may function as a neuroprotective agent against sevoflurane-induced neurotoxicity.

Are intrinsic neural timescales related to sensory processing? Evidence from abnormal behavioral states.

The brain exhibits a complex temporal structure which translates into a hierarchy of distinct neural timescales. An open question is how these intrinsic timescales are related to sensory or motor information processing and whether these dynamics have common patterns in different behavioral states. We address these questions by investigating the brain's intrinsic timescales in healthy controls, motor (amyotrophic lateral sclerosis, locked-in syndrome), sensory (anesthesia, unresponsive wakefulness syndrome), and progressive reduction of sensory processing (from awake states over N1, N2, N3). We employed a combination of measures from EEG resting-state data: auto-correlation window (ACW), power spectral density (PSD), and power-law exponent (PLE). Prolonged neural timescales accompanied by a shift towards slower frequencies were observed in the conditions with sensory deficits, but not in conditions with motor deficits. Our results establish that the spontaneous activity's intrinsic neural timescale is related to the neural capacity that specifically supports sensory rather than motor information processing in the healthy brain.

A unique formyl iodoargentate exhibiting luminescent and photocurrent response properties.

A decomposition and self-assembly reaction affords a novel formyl iodoargentate [H2L]n[Ag2I3(µ-CHO)]n (1, L = 2,6-bis(1-imdazoly)pyridine) with an unprecedented CHO- link mode, which provides the only example of iodoargentate incorporating the unstable formyl species under hydrothermal conditions. 1 exhibits luminescent and photocurrent response properties.

A series of new hybrid chalcogenogermanates: the rare examples of chalcogenogermanates combined with trivalent vanadium complexes.

A series of new hybrid chalcogenogermanates [Mn2(en)4Ge2S6]n (1, en = ethylenediamine), [Mn2(dap)4Ge2S6]n (2, dap = 1,2-diaminopropane), [H2dien]n[MnGeS4]n (3, dien = diethylenetriamine), [V(en)2(ea)]2[Ge2Se6] (4, Hea = ethanolamine), [V(teta)(ea)]2[Ge2Se6] (5, teta = triethylenetetramine) and [V2(en)6(µ-O)][Ge2Se6] (6) were solvothermally synthesized and structurally characterized. Both 1 and 2 contain dimeric [Ge2S6]4- anions and [Mn(en)2]2+/[Mn(dap)2]2+ complex cations, which are interconnected to generate 1-D neutral chain-like structures [Mn2(en)4Ge2S6]n and [Mn2(dap)4Ge2S6]n, respectively. 3 consists of a protonated H2dien2+ cation and a 1-D straight chain built from [MnS4] and [GeS4] tetrahedra sharing opposite edges, and is the only example of a chelating amine uncoordinated to a transition metal ion. Both 4 and 5 consist of [Ge2Se6]4- anions constructed by two [GeSe4] tetrahedra sharing a common edge and discrete complex cations [V(en)2(ea)]2+/[V(teta)(ea)]2+. 6 is composed of a [Ge2Se6]4- anion and dinuclear complex cation [V2(en)6(µ-O)]4+ containing an en molecule as a rare monodentate ligand. Although some selenidogermanates with transition metal complexes have been successfully prepared, no selenidogermanates with trivalent vanadium complexes have been documented. Therefore, 4-6 offer the first examples of selenidogermanates with trivalent vanadium complexes under solvothermal conditions. Their optical and photocurrent response properties were studied.

An unusual cuprous iodide polymer incorporating I-, I2 and I3- structural units.

A novel cuprous iodide polymer [Cu2(µ4-L)(µ2-I-)(I2)(I3-)]n·nI2 (1, L = 1-tetrazole-4-imidazole-benzene) has been hydrothermally synthesized. 1 contains a 1-D chain [Cu2(µ4-L)(µ2-I-)(I2)(I3-)]n and free I2 molecules, which are interconnected by weak II interactions to form an unprecedented 3-D cuprous polyiodide network containing rare 1-D [(I-)·2I2·(I3)-]n polyiodide ribbons. 1 shows luminescence and photoelectronic properties, and its theoretical band structure was also studied.

Lycium barbarum polysaccharide protects against neurotoxicity via the Nrf2-HO-1 pathway.

The incidence of neurodegenerative diseases including Alzheimer's and Parkinson's disease has markedly increased over the past few decades. Oxidative stress is considered to be a common pathophysiological condition resulting in neurotoxicity. Lycium barbarum polysaccharide (LBP) is the major active component of Lycium barbarum L., which exhibit potent antioxidant activity. The current study investigated the neuroprotective effects of LBP in H2O2-treated PC12 cells in vitro and in CoCl2-treated rats in vivo. It was determined that LBP concentration-dependently reversed the H2O2-induced increase in reactive oxygen species (ROS) levels, decrease in cell viability, increase in TUNEL-stained cells, increase in caspase-3 and -9 activity and decrease in mitochondrial membrane potential, indicating the amelioration of mitochondrial apoptosis. Furthermore, LBP inhibited the H2O2-induced decrease in nuclear factor erythroid 2-related factor 2 (Nrf)2 and heme oxygenase (HO)-1 expression and binding of Nrf2 to the promoters of HO-1. Silencing of Nrf2 and inhibition of HO-1 by zinc protoporphyrin IX (ZnPP) reversed the protective effects of LBP against H2O2-resulted neurotoxicity in PC12 cells. In CoCl2-treated rats, it was demonstrated that LBP decreased brain tissue apoptosis, reduced the time spent by rats finding the platform site, decreased escape latencies and reduced the distance traveled to find the platform. In addition, LBP inhibited the CoCl2-induced decrease of Nrf2 and HO-1 expression. Administration of ZnPP also suppressed the protective effects of LBP against CoCl2-resulted neurotoxicity in rats. Thus, the current study indicated that LBP exhibits protective effects against neurotoxicity by upregulating Nrf2/HO-1 signaling. These data may increase understanding regarding the neuroprotective activities of LBP.