ABSTRACT
Hantavirus is the causative agent of hemorrhagic fever with renal syndrome (HFRS). Heilongjiang Province is experiencing an epidemic of HFRS, the main causative agent is a variant of hantavirus called Seoul virus (SEOV). In this study, the entire genome of one SEOV, the DN2 strain, was sequenced and analyzed. The alignment analysis of the sequences indicated that the DN2 strain shares the highest homology with the SEOV-LYO852 strain. The nucleotide identity is 97.6% for the S segment, 97.7% for the M segment, and 98.0% for the L segment. The corresponding amino acid sequence homologies are 99.1%, 98.9% and 99.8%. The phylogenetic analysis of the segments suggests that the DN2 strain has a high genetic relationship with SEOV strains and no genetic recombination occurs.
L'Hantavirus est l'agent causal de la fièvre hémorragique avec syndrome rénal (FHSR). La province d'Heilongjiang est au prise avec une épidémie de FHSR, l'agent causal principal est un variant de l'Hantavirus dénommé virus Séoul (SEOV). Dans la présente étude, les séquences complètes d'un SEOV, la souche DN2, ont été séquencées et analysées. L'analyse d'appariement des séquences a démontré que la souche DN2 partage la plus forte homologie avec la souche SEOV-LYO852. L'identité de nucléotides est de 97,6 % pour le segment S, 97,7 % pour le segment M, et de 98,0 % pour le segment L. L'homologie des séquences d'acides aminés correspondants est de 99,1 %, 98,9 %, et 99,8 %. L'analyse phylogénétique des segments suggéraient que la souche DN2 avait une parenté génétique la plus élevée avec les souches de SEOV et qu'aucune recombinaison génétique ne s'est produite.(Traduit par Docteur Serge Messier).
Subject(s)
Genome, Viral/genetics , Hemorrhagic Fever with Renal Syndrome/virology , Seoul virus/genetics , Animals , China/epidemiology , Hemorrhagic Fever with Renal Syndrome/epidemiology , Phylogeny , RNA, Viral/genetics , Rats , Seoul virus/classification , Sequence Alignment , Sequence Analysis , Sequence Homology, Amino Acid , Sequence Homology, Nucleic AcidABSTRACT
BACKGROUND: Hantavirus is a tripartite negative-sense RNA virus. It can infect humans through contaminated rodent excreta and causes two types of fatal human diseases: hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). China exhibits the highest HFRS occurrence rate in the world, and the Heilongjiang area is one of the most severely infected regions. RESULTS: To obtain additional insights into the genetic characteristics of hantaviruses in the port cities of the Heilongjiang area in China, a molecular epidemiological investigation of hantaviruses isolated from rodents was performed in 2014. A total of 649 rodents (11 murine species and 1 shrew species) were caught in 12 port cities in Heilongjiang. Among these rodents, the most common species was A. agrarius, and the second-most common was R. norvegicus. A viral gene PCR assay revealed the presence of two specific genotypes of hantavirus, referred to as Hantaan virus (HTNV) and Seoul virus (SEOV), and the positive SEOV infection rate was higher than that for HTNV. A genetic analysis based on partial M segment sequences indicated that all of the isolates belonging to SEOV could be assigned to two genetic lineages, whereas the isolate belonging to HTNV could be assigned to only one genetic lineage. CONCLUSIONS: These results suggested that HTNV and SEOV are circulating in A. agrarius and R. norvegicus in the port cities in the area of Heilongjiang, but SEOV may be the dominant common hantavirus.
