ABSTRACT
BACKGROUND: This work aimed to investigate the potential benefits of administering Prevotella and its primary metabolite succinate on performance, hepatic lipid accumulation and gut microbiota in laying hens. RESULTS: One hundred and fifty 58-week-old Hyline Brown laying hens, with laying rate below 80% and plasma triglyceride (TG) exceeding 5 mmol/L, were used in this study. The hens were randomly allocated into 5 groups and subjected to one of the following treatments: fed with a basal diet (negative control, NC), oral gavage of 3 mL/hen saline every other day (positive control, PC), gavage of 3 mL/hen Prevotella melaninogenica (107 CFU/mL, PM) or 3 mL/hen Prevotella copri (107 CFU/mL, P. copri) every other day, and basal diet supplemented with 0.25% sodium succinate (Succinate). The results showed that PM and P. copri treatments significantly improved laying rate compared to the PC (P < 0.05). The amount of lipid droplet was notably decreased by PM, P. copri, and Succinate treatments at week 4 and decreased by P. copri at week 8 (P < 0.05). Correspondingly, the plasma TG level in Succinate group was lower than that of PC (P < 0.05). Hepatic TG content, however, was not significantly influenced at week 4 and 8 (P > 0.05). PM treatment increased (P < 0.05) the mRNA levels of genes PGC-1ß and APB-5B at week 4, and ACC and CPT-1 at week 8. The results indicated enhanced antioxidant activities at week 8, as evidenced by reduced hepatic malondialdehyde (MDA) level and improved antioxidant enzymes activities in PM and Succinate groups (P < 0.05). Supplementing with Prevotella or succinate can alter the cecal microbiota. Specifically, the abundance of Prevotella in the Succinate group was significantly higher than that in the other 4 groups at the family and genus levels (P < 0.05). CONCLUSIONS: Oral intake of Prevotella and dietary supplementation of succinate can ameliorate lipid metabolism of laying hens. The beneficial effect of Prevotella is consistent across different species. The finding highlights that succinate, the primary metabolite of Prevotella, represents a more feasible feed additive for alleviating fatty liver in laying hens.
ABSTRACT
Clostridium butyricum is an important probiotic for chickens and exerts various biological activities, including altering the composition of the intestinal microbiota, competing with other microorganisms for nutrients, improving the integrity of the intestinal mucosal system, changing the intestinal barrier, and improving overall host health. Intestinal microbes also play vital roles in maintaining the intestinal barrier, regulating intestinal health, and promoting chicken growth. During chicken production, chickens are vulnerable to various stressors that have detrimental effects on the intestinal barrier with significant economic consequences. C. butyricum is a known probiotic that promotes intestinal health and produces the short-chain fatty acid butyric acid, which is beneficial for the growth performance of chickens. This review elucidates the development and utilization of C. butyricum to improve intestinal barrier function and growth performance in chickens through its probiotic properties and interactions with intestinal microbes.
