ABSTRACT
The Internet Data Center (IDC) is one of the most important infrastructures in the field of information technology. The cooling system for heat dissipation of IDC is indispensable due to it generates a large amount of heat during its calculation process, which may potentially harm its normal operation. Electronic fluorinated fluids have been widely used in cooling systems of IDC with stable physical and chemical properties. However, the biological toxicity of electronic fluorinated fluids has not been fully evaluated and there is a lack of unified safety standards, which may pose potential risks to the environment and human health. Here, hexafluoropropylene terpolymer (HFPT) as an example has been systematically studied, fully considering the application scenarios of data centers. Also, the emergency effects of fluorinated coolants in mammalian models from the perspectives of inhalation, skin contact, accidental entry into eyes, accidental ingestion, and chronic toxicity, are evaluated. Multiple in vivo experiments have proven that HFPT not only has stable physical and chemical properties, that can maintain the safe operation of IDC, but also has low physiological toxicity to mammals and can provide health benefits to data center staff and the assurance of surrounding environment. This study proves the good biological safety of electronic fluorinated fluids and provides a reference for environmental assessment and risk management of liquid cooling technology in IDC. Supplementary Information: The online version contains supplementary material available at 10.1007/s43188-024-00234-3.
ABSTRACT
As a developing radiation treatment for tumors, neutron capture therapy (NCT) has less side effects and a higher efficacy than conventional radiation therapy. Drugs with specific isotopes are indispensable counterparts of NCT, as they are the indespensable part of the neutron capture reaction. Since the creation of the first and second generations of boron-containing reagents, NCT has significantly advanced. Notwithstanding, the extant NCT medications, predominantly comprised of small molecule boron medicines, have encountered challenges such monofunctionality, inadequate targeting of tumors, and hypermetabolism. There is an urgent need to promote the research and development of new types of NCT drugs. Bio-nanomaterials can be introduced into the realm of NCT, and nanotechnology can give conventional medications richer functionality and significant adaptability. This can complement the advantages of each other and is expected to develop more new drugs with less toxicity, low side effects, better tumor targeting, and high biocompatibility. In this review, we summarized the research progress of nano-drugs in NCT based on the different types and sources of isotopes used, and introduced the attempts and efforts made by relevant researchers in combining nanomaterials with NCT, hoping to provide pivotal references for promoting the development of the field of tumor radiotherapy.
Subject(s)
Neoplasms , Humans , Neoplasms/radiotherapy , Neoplasms/drug therapy , Animals , Neutron Capture Therapy/methods , Nanoparticles/chemistry , Nanostructures/therapeutic use , Nanostructures/chemistry , Nanotechnology/methods , Boron Neutron Capture Therapy/methods , Boron Compounds/therapeutic use , Boron Compounds/chemistry , Boron Compounds/pharmacologyABSTRACT
With the popularity and development of electronic devices, the demand for lithium batteries is increasing, which also puts high demands on the energy density, cycle life and safety of lithium batteries. Gel electrolytes achieve both of these requirements by curing the electrolytes to reduce the interfacial side reactions of lithium metal batteries. The ionic conductivity of the gel electrolytes prepared by inâ situ curing reach 8.0×10-4 â S cm-1 , and the ionic mobility number is 0.53. Meanwhile, the gel electrolytes maintain a stable electrochemical window of 1.0-5.0â V. Benefited with the interfacial regulation of PEGDA gel electrolytes, the gel lithium metal batteries show better cycling stability, and achieved 97 % capacity retention after 200 cycles (0.2â C) with a lower increasing rate of impedance.
ABSTRACT
Rechargeable lithium metal batteries (LMBs) offer excellent opportunities for applications requiring high-energy-density battery systems. So far, it has received a lot of interest in pairing higher-energy-density high-voltage nickel-rich cathodes. Here, fluorinated solvents were used instead of the usual carbonate solvents to prepare gel polymer electrolytes (FGPE) by in situ polymerization of polymers introducing the fluorine-containing groups. Theoretically and experimentally, FGPE has proven to be ultra-compatible with the lithium metal anode and LiNi0.8Co0.1Mn0.1O2 cathode. A stable plating/stripping process of over 2000 h can be achieved for symmetrical lithium cells using FGPE. The Li||FGPE||NCM811 cell has a longer cycle life at a high voltage (4.5 V). In addition, the zero self-extinguishing time indicates that the FGPE has sufficient safety. In summary, the design of this electrolyte provides ideas to improve the safety and energy density of LMBs.
ABSTRACT
In response to the low removal capacity and poor tolerance of fungi to diesel-contaminated soil, a novel immobilization system using biochar to enhance composite fungi was proposed. Rice husk biochar (RHB) and sodium alginate (SA) were used as immobilization matrices for composite fungi, and the adsorption system (CFI-RHB) and the encapsulation system (CFI-RHB/SA) were obtained. CFI-RHB/SA exhibited the highest diesel removal efficiency (64.10%) in high diesel-contaminated soil over a 60-day remediation period compared to the free composite fungi (42.70%) and CFI-RHB (49.13%). SEM demonstrated that the composite fungi were confirmed to be well attached to the matrix in both CFI-RHB and CFI-RHB/SA. FTIR analysis revealed the appearance of new vibration peaks in diesel-contaminated soil remediated by immobilized microorganisms, demonstrating changes in the molecular structure of diesel before and after degradation. Furthermore, CFI-RHB/SA maintains a stable removal efficiency (>60%) in higher concentrations of diesel-contaminated soil. High-throughput sequencing results indicated that Fusarium and Penicillium played a key role in the removal of diesel contaminants. Meanwhile, both dominant genera were negatively correlated with diesel concentration. The addition of exogenous fungi stimulated the enrichment of functional fungi. The insights gained from experiment and theory help to provide a new understanding of immobilization techniques of composite fungi and the evolution of fungal community structure.
Subject(s)
Oryza , Soil Pollutants , Biodegradation, Environmental , Oryza/chemistry , Soil Pollutants/analysis , Fungi , Soil/chemistryABSTRACT
This study investigated the effect of Ca(H2PO4)2 on pH, leaching toxicity and speciations of soil before and after leaching on it. Different amounts of Ca(H2PO4)2 were added to Pb-contaminated soil and stabilized for 30 days. The changes of pH and leaching toxicity of Pb-contaminated soil were tracked during that period. The content of Pb in soil before and after leaching was also determined after 30 days of stabilization. Results showed that the pH of the Pb-contaminated soil didn't change much with the addition of-Ca(H2PO4)2. When the amount of Ca(H2PO4)2 reached to 3 wt%, the leaching toxicity met the standard limiting level of groundwater class III of China. The change of leaching toxicity was found to be mainly affected by the water-soluble fraction and mild acid-soluble fraction of lead. The speciation experiments revealed that the changes on reducible, oxidizable, and residual fractions are significant, while there are only minor changes on the water-soluble and mild acid-soluble fractions. X-ray diffractometry (XRD) analysis showed that Pb9(PO4)6 and Pb2P2O7 substances were generated in the stabilized soil. The stabilization mechanism of Ca(H2PO4)2 was mainly attributed to the formation of insoluble Pb phosphate precipitates through interactions between the heavy metal Pb and the Ca(H2PO4)2. In such a way the active species of Pb in the soil can be successfully stabilized. Novelty statementAt present, the leaching toxicity is currently used for the evaluation of stabilization effect of heavy metal contaminated soil. The speciation distribution of stabilized contaminated soil before and after leaching has rarely been studied, and the research on stabilizing contaminated soil after leaching is less.Therefore, this paper mainly studies the stabilization effect through the speciation changes of contaminated soil before and after leaching, providing a new idea and method for the evaluation of the stabilization effect of contaminated soil remediation.Ca(H2PO4)2 has no significance in pH of contaminated soil: 5.05ï¼pHï¼5.5.The content of the water-soluble fraction and the mild acid-soluble fraction of Pb were availably reduced by Ca(H2PO4)2.The content of the water-soluble fraction and the mild acid-soluble fraction of Pb has no marked change before and after leaching.The stabilization mechanism of Ca(H2PO4)2 is through interaction between the Pb in the soil and phosphate to form insoluble substances of lead phosphate.Ca(H2PO4)2 has a good effect on the stabilization of lead-contaminated soil.
Subject(s)
Soil Pollutants , Biodegradation, Environmental , Calcium/analysis , Lead , Phosphates/analysis , Phosphates/chemistry , Soil/chemistry , Soil Pollutants/analysisABSTRACT
The ion intercalation behavior in 2D materials is widely applied in energy storage, electrocatalysis, and desalination. However, the detailed effect of ions on the performance, combining the influence of interlayer force and the change of solvent shell, is far less well understood. Here the solvated alkali metal ions with different sizes are intercalated into the lattice of 2D materials with different spacings (Ti3 C2 Tx , δ-MnO2 , and reduced graphene oxide) to construct the intercalation model related with sub-nanometer confined ions and solvent molecules to further understand the intercalation capacitance. Based on electrochemical methods and density functional theory calculation, the ions lose the electrostatic shielding solvent shell or shorten the distance between the layers, resulting in a significant increase in capacitance. It is found that the intercalation capacitance arises from the diffusion of solvated ions and is controlled by quantum and electrochemical capacitance for desolvated ions. This effect of solvation structure on performance can be applied in a variety of electrochemical interface studies and provides a new research view for energy storage mechanisms.
ABSTRACT
Aprotic lithium-oxygen batteries currently suffer from poor cyclic stability and low achievable energy density. Herein, gold nanoparticles capped with mercaptosuccinic acid are dispersed in 1.0 M LiClO4/dimethyl sulfoxide (DMSO) as a novel electrolyte for lithium-oxygen batteries. Morphological and electrochemical analyses indicate that film-like amorphous lithium peroxide is formed using the gold nanocolloid electrolyte instead of bulk crystals in battery discharging, which apparently increases the conductivity and accelerates the decomposition kinetics of discharge products in recharging, accompanied by the release of incorporated gold nanoparticles with the decomposition of lithium peroxide into the electrolyte. Experiments and theoretical calculations further demonstrate that the suspended gold nanoparticles in the electrolyte can adsorb some intermediates generated by an oxygen reduction reaction, which effectively alleviates the cleavage of the electrolyte and impedes the corrosion of the lithium anode. As a result, the life span of lithium-oxygen batteries is dramatically increased from 55 to 438 cycles, and the rate performance and full-discharge capacity are also massively enhanced. The battery failure is attributed to the degradation of gold nanocolloid electrolytes, and further studies on improvement of colloid stability during battery cycling are underway.
ABSTRACT
Residual electrolyte is the main pollution source in the lithium ion battery disassembly process. A practical detoxified approach is studied using the lithium hexafluorophosphate in the decommissioned power battery with dimethyl carbonate as a solvent. The pH measurement, Fourier transform infrared spectroscopy, micromorphology and phase structure characterization techniques showed that the process in this study is capable of removing lithium hexafluorophosphate from decommissioned power batteries, while controlling the proper ratio of NaOH can also completely precipitate F- into CaF2 crystal and allows recycling of the organic solvents. This process scheme of residual electrolyte treatment effectively reduces environmental pollution during the decommissioned power batteries recycling process, and has the benefit of resource reuse for valuable elements.
Subject(s)
Electric Power Supplies , Lithium , Electrolytes , Recycling , SolventsABSTRACT
A multifunctional PC10A/DOX/MoS2 hydrogel was designed and prepared for chemotherapy/photothermal therapy/photodynamic therapy of 4T1 tumor, and the immune responses triggered by photothermal and photodynamic effect of MoS2 nanosheet in the hydrogel were also studied. Positively charged DOX and negatively charged PC10A were loaded on the surface of MoS2 nanosheet through layer-by-layer method to prepare hybrid PC10A/DOX/MoS2 nanoparticles. PC10A/DOX/MoS2 nanoparticles were dispersed in PC10A hydrogel to prepare PC10A/DOX/MoS2 hydrogel. 2D MoS2 nanosheet in the hydrogel was simultaneously utilized as photothermal agent and photodynamic agent for the generation of hyperthermia and reactive oxygen species, respectively. This PC10A/DOX/MoS2 hydrogel was injectable and possessed excellent biocompatibility. The results of in vivo tumor-bearing mice experiments showed that a remarkably enhance tumor inhibition was observed by the combination of chemo-photothermal-photodynamic therapy compared with photothermal therapy, photodynamic therapy, or chemotherapy alone. In addition, the results of in vivo therapy exhibited that the PC10A/DOX/MoS2 hydrogel with laser irradiation could activate antitumor immune effects to suppress the growth of primary 4T1 breast tumors and distal lung metastatic nodules. Therefore, these results demonstrated that the PC10A/DOX/MoS2 hydrogel was promising to be utilized in antitumor immunity therapy triggered by photothermal therapy and photodynamic therapy for malignant tumor.
Subject(s)
Breast Neoplasms/therapy , Doxorubicin/administration & dosage , Lung Neoplasms/secondary , Lung Neoplasms/therapy , Photochemotherapy/methods , Photothermal Therapy/methods , Tumor Escape/drug effects , Animals , Breast Neoplasms/immunology , Cell Line, Tumor , Disulfides , Doxorubicin/chemistry , Doxorubicin/pharmacology , Drug Therapy , Female , Humans , Hydrogels , Injections , Lung Neoplasms/immunology , Mice , Molybdenum , Nanoparticles , Peptides , Xenograft Model Antitumor AssaysABSTRACT
Polypyrrole encapsulated nickel nanorods (PPy@Ni) have been prepared by electro-polymerization using Ni nanorods as template. PPy@Ni were characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman spectrometer, Fourier transform infrared (FTIR) spectra and vibrating sample magnetometry (VSM). The maximum saturation magnetization of PPy@Ni was up to 44.6 emu g-1. Further, PPy@Ni was used as the additive for the conductivity improvement in polymer matrix. Experimental results showed that the electric conductivity reached 1.8×10-3 S m-1 when 5% PPy@Ni was added in the poly(urethane acrylate) matrix by applying a magnetic field. Further tests showed that when the additive concentration increased from 0.1% to 5%, the correspondence saturation magnetization increased from 15.1 emug-1 to 31.3 emug-1. This work provides new light on controllable fabrication of conducting polymer by adding magnetic alignment conductive additives.
ABSTRACT
Simple low rate pre-activation effectively prolonged the cycle life of Li-O2 batteries with MWNT cathodes in a 1 M LiClO4/DMSO electrolyte from 55 to 290 cycles, and the ultimate capacity and rate performance were also significantly enhanced, attributed to reconstructed homogeneous and compact SEI layers on the Li anodes by pre-activation.
ABSTRACT
It is a challenging task to prepare well-dispersed and highly luminescent quantum dots (QDs) powder and a new strategy is reported in this article. Sodium carboxymethyl starch (CMS-Na) was employed in this work to prepare the QDs-starch composite. Ultraviolet (UV) light shows that the blank starches had no fluorescence, while the QDs-starches were highly luminescent. Scanning electron microscopy (SEM) observation shows that the QDs-starch composite has the typical particle morphology with the diameter around 200 nm. Energy dispersive X-ray spectroscopy (EDX) results show that there are intensive tellurium (Te) and cadmium (Cd) element signals. Combined fluorescent lifetime and steady-state spectrometer show that the QDs-starch quantum yields (QYs) increase when the QDs loading increases from 1 × 10-6 mol/g to 2 × 10-6 mol/g, but when the loadings further increase, the QYs decrease slightly. For the red colour (λem = 660 nm) QDs, the QYs can reach to as high as 28.2%, and for the other colour QDs they can also have the QYs above 22%. Time-resolved photobleaching experiments show that the fluorescent QDs-starch composite has a half-decay time of 40.23 s. These results indicate that the CMS-Na is a promising QDs dispersant to obtain high QY QD composites.
Subject(s)
Luminescence , Quantum Dots/chemistry , Quantum Theory , Starch/analogs & derivatives , Starch/chemistry , Ultraviolet RaysABSTRACT
Here, we report the synthesis and characterization of CoFe2O4 doping Ag2S dendrimer-modified nanoparticles (CoFe2O4-Ag2S DMNs) in Cry1Ab protein detection and imaging. The near-infrared Ag2S quantum dots were first prepared by using the thermal decomposition method, followed by modification of the water-soluble quantum dots using the method of solvent evaporation and ligand exchange, and finally the fluorescent magnetic bifunctional nanoparticles were obtained by binding with CoFe2O4. As-prepared CoFe2O4-Ag2S DMNs were characterized by fluorescence (FL) spectroscopy and transmission electron microscopy (TEM). Results showed that Ag2S DMNs could sensitively detect Cry1Ab both in vitro and in vivo. In vitro, the enhanced FL intensity as a function of the concentration is notably consistent with the Langmuir binding isotherm equation in the range of 0-200 ng/mL of Cry1Ab proteins. The detection limit of this method was found to be 0.2 ng/mL. Meanwhile, the fluorescence wavelength was extended to the second near-infrared range (NIR-II, 1.0~1.4 µm), which enables in vivo imaging. This study highlights the importance of NIR QDs doping magnetic materials as a new method to trace Bacillus thuringiensis (Bt) in insects and their potential applications in in vivo NIR tissue imaging.
Subject(s)
Bacillus thuringiensis/isolation & purification , Bacterial Proteins/analysis , Cobalt/chemistry , Endotoxins/analysis , Ferric Compounds/chemistry , Fluorescent Dyes/chemistry , Hemolysin Proteins/analysis , Nanoparticles/chemistry , Silver Compounds/chemistry , Animals , Bacillus thuringiensis Toxins , Dendrimers/chemical synthesis , Dendrimers/chemistry , Fluorescent Dyes/chemical synthesis , In Vitro Techniques , Larva , Microscopy, Electron, Transmission , Moths , Particle Size , Spectrometry, Fluorescence , Surface PropertiesABSTRACT
Nitrogen doped carbon nanocage with graphitic shell (NGCS) was fabricated through in-situ solid reaction between calcium acetate and dicyandiamide in an inert atmosphere followed by acid etching. The role played by the calcium acetate (Ca(Ac)2) and dicyandiamide (DCD) during the synthesis process is one-stone-two-birds. Calcium acetate plays multiple functions: template agent, graphitization catalyst, and carbon source. Dicyandiamide can be considered as the nitrogen sources and the chemical reaction agent that can be reacted with calcium acetate to form it into CaCN2. The NGCS obtained at 800 °C has a specific surface area of 420 m2/g and nitrogen content of 8.87 at%. The excellent electrochemical performance can be attributed to the combination effects of porous structure, nitrogen doping and graphitized nanocage shell of NGCS electrode. The hollow structure serves as the reservoir for fast electrolyte ion supplement. Nitrogen groups not only improve the wettability of interfaces between carbon surface and electrolyte, but also generate extra pseudocapacitance through redox reaction. The graphitic carbon nanocage shell can enhance the conductivity and facilitates the fast charge transfer. At a current density of 0.5 A/g, the specific capacitance of the NGCS-800 electrode is 215 F/g. Furthermore, the NGCS-800 electrode exhibits excellent rate capability (80% capacitance retention at 10 A/g) and outstanding cycling stability (96.89% capacitance retention after 5000 cycles). These intriguing results demonstrate that nitrogen doped carbon with graphitic shell will be highly promising as electrode materials for supercapacitors and other energy storage and conversation applications.
ABSTRACT
It is of great significance to seek high-performance solid electrolytes via a facile chemistry and simple process for meeting the requirements of solid batteries. Previous reports revealed that ion conducting pathways within ceramic-polymer composite electrolytes mainly occur at ceramic particles and the ceramic-polymer interface. Herein, one facile strategy toward ceramic particles' alignment and assembly induced by an external alternating-current (AC) electric field is presented. It was manifested by an in situ optical microscope that Li1.3Al0.3Ti1.7(PO4)3 particles and poly(ethylene glycol) diacrylate in poly(dimethylsiloxane) (LATP@PEGDA@PDMS) assembled into three-dimensional connected networks on applying an external AC electric field. Scanning electron microscopy revealed that the ceramic LATP particles aligned into a necklacelike assembly. Electrochemical impedance spectroscopy confirmed that the ionic conductivity of this necklacelike alignment was significantly enhanced compared to that of the random one. It was demonstrated that this facile strategy of applying an AC electric field can be a very effective approach for architecting three-dimensional lithium-ion conductive networks within solid composite electrolyte.
ABSTRACT
BACKGROUND: Ag2S has the characteristics of conventional quantum dot such as broad excitation spectrum, narrow emission spectrum, long fluorescence lifetime, strong anti-bleaching ability, and other optical properties. Moreover, since its fluorescence emission is located in the NIR-II region, has stronger penetrating ability for tissue. Ag2S quantum dot has strong absorption during the visible and NIR regions, it has good photothermal and photoacoustic response under certain wavelength excitation. RESULTS: 200 nm aqueous probe Ag2S@DSPE-PEG2000-FA (Ag2S@DP-FA) with good dispersibility and stability was prepared by coating hydrophobic Ag2S with the mixture of folic acid (FA) modified DSPE-PEG2000 (DP) and other polymers, it was found the probe had good fluorescent, photoacoustic and photothermal responses, and a low cell cytotoxicity at 50 µg/mL Ag concentration. Blood biochemical analysis, liver enzyme and tissue histopathological test showed that no significant influence was observed on blood and organs within 15 days after injection of the probe. In vivo and in vitro fluorescence and photoacoustic imaging of the probe further demonstrated that the Ag2S@DP-FA probe had good active targeting ability for tumor. In vivo and in vitro photothermal therapy experiments confirmed that the probe also had good ability of killing tumor by photothermal. CONCLUSIONS: Ag2S@DP-FA was a safe, integrated diagnosis and treatment probe with multi-mode imaging, photothermal therapy and active targeting ability, which had a great application prospect in the early diagnosis and treatment of tumor.
Subject(s)
Molecular Probes , Optical Imaging/methods , Photoacoustic Techniques/methods , Quantum Dots , Silver Compounds , A549 Cells , Animals , HeLa Cells , Humans , Hydrophobic and Hydrophilic Interactions , Male , Mice , Mice, Inbred BALB C , Molecular Probes/chemistry , Molecular Probes/toxicity , Phosphatidylethanolamines/chemistry , Phosphatidylethanolamines/toxicity , Phototherapy , Polyethylene Glycols/chemistry , Polyethylene Glycols/toxicity , Quantum Dots/chemistry , Quantum Dots/toxicity , Silver Compounds/chemistry , Silver Compounds/toxicityABSTRACT
Graphene quantum dot (GQD) has been attractive in analytical science field due to its low toxicity, stable photoluminescence. Herein, nitrogen-doped GQD (N-GQD) was prepared by a facile solvothermal treatment of GO using dimethylformamide, and exhibited a green emission with 23.1% quantum yield. The N-GQD probe showed a selective and sensitive fluorescence enhancement response to Al3+, the mechanism might be the formation of a complex between Al3+ and N-GQD constrained the photo-induced electron transfer (PET) process of N-GQD itself. With Benesi-Hildebrand equation, the binding constant and molar ratio between N-GQD and Al3+ was calculated to be 4.6 × 104Lmol-1 and 1:1 respectively. The pKa value of N-GQD was also determined to be 4.4 by capillary electrophoresis. In pH 4.0 PBS solution, there was a good linear relation between the fluorescence intensity and the logarithm of concentration of Al3+ in the range of 2.5-75µmolL-1, the limit of detection (3σ) was 1.3µmolL-1. This "Off - On" fluorescence method had been applied to accurate quantification of aluminum in hydrotalcite tablets. What's more, the fluorescence switch property of N-GQD was explored by alternate addition of Al3+ and EDTA. The probe was also utilized for detection Al3+ in living cells due to its excellent biocompatibility.
Subject(s)
Aluminum/analysis , Graphite/chemistry , Nitrogen/chemistry , Optical Imaging/methods , Quantum Dots/chemistry , Spectrometry, Fluorescence/methods , Aluminum Hydroxide/chemistry , Antacids/chemistry , Biosensing Techniques/methods , Cations/analysis , Fluorescence , HeLa Cells , Humans , Limit of Detection , Magnesium Hydroxide/chemistry , Quantum Dots/ultrastructure , Tablets , Water/analysisABSTRACT
In this paper, visual detection of trace lead ion was established by aptamer and silver staining. The basic strategy was that aminated PS2.M aptamer was immobilized onto slide and formed stable G-quadruplex structure. PbS was generated by adding S2-, and it catalyzed subsequent silver staining reaction, through the silver staining amplification effect, the slide presented visible ash black. The gray value of slide after silver staining was analyzed and the semi-quantitative detection of Pb2+ in solution was realized. The results showed that optical darkness ratio (ODR) and logarithmic value of Pb2+ concentration had a good linear relationship (R2â¯=â¯0.951) over the range of 0.5-10⯵M. In addition, there was no obvious interference of other common metal ions for the detection, indicating that this method presented outstanding selectivity. And it was also used for qualitative and semi-quantitative determination of Pb2+ in soil sample successfully.
Subject(s)
Aptamers, Nucleotide/chemistry , Biosensing Techniques , Environmental Pollutants/analysis , Lead/analysis , Metal Nanoparticles/chemistry , Silver/chemistry , Cations, Divalent , Colorimetry/methods , Humans , Limit of Detection , Silver Staining/instrumentation , Silver Staining/methodsABSTRACT
In this paper, gold nanorods and InP/ZnS quantum dots were encapsulated together in a silica medium, and the targeting molecular peptide c(RGDfC) was further connected after surface modification with PEG and PEG derivatives to prepare a multifunctional Au@QD@SiO2/PEG-c(RGDfC) probe. Dynamic Light Scattering showed that the probe size was about 215.01 ± 2.72 nm, and its dispersibility was good. In in vitro experiments when the concentration was as high as 200 µg mL-1, the activity of the cells was still 85% due to low toxicity. In vivo experiments showed that the probe had excellent tumor targeting, X-ray computed tomography (CT) imaging and fluorescence imaging capabilities. The experiments revealed that the probe had a long blood circulation time (T1/2 = 7.78 h) in mice. Biochemical analysis, liver enzyme analysis and histomorphological analysis after probe injection showed that the probe had no obvious side effects on the normal functions of the main organs, indicating good biosafety. In vivo imaging experiments showed that 6 d after intravenous injection, the tumor sites of a HeLa tumor-bearing nude mice positive group presented obvious fluorescence and CT signals, indicating that the prepared nanoprobe had good tumor targeting dual-mode imaging capabilities and therefore showed great potential in biomedical imaging applications, especially the diagnosis of cancer.