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1.
Mem Inst Oswaldo Cruz ; 96(1): 71-9, 2001 Jan.
Article in Portuguese | MEDLINE | ID: mdl-11285477

ABSTRACT

The authors describe a new species of Triatominae (Hemiptera, Reduviidae) from the State of Rio Grande do Sul, Brazil. The study was made with specimens captured in basaltic formations, at an average altitude of 750 m.o.s.l. The new species is included in the oliveirai complex together with other four species T. williami, T. matogrossensis, T. guazu and T. jurbergi). The new species was compared with the most similar one, T. oliveirai, from which the main differences are on the proportions of head, ante and postocular regions, the general color pattern and the male genitalia, specially on the median process of the pygophore, the support of phallosome, the vesica and the process of the endosome.


Subject(s)
Triatominae/classification , Animals , Brazil , Female , Male , Triatominae/anatomy & histology , Triatominae/genetics
2.
Mem Inst Oswaldo Cruz ; 95(6): 795-800, 2000.
Article in English | MEDLINE | ID: mdl-11080763

ABSTRACT

Between 1984 and 1993 the prevalence of the Trypanosoma cruzi infection in opossums (Didelphis marsupialis) was studied in Santa Catarina and Arvoredo Islands, State of Santa Catarina, Brazil. The association of the triatomine bug Panstrongylus megistus with opossums nests and the infection rate of these triatomines by T. cruzi was also studied. Thirteen different locations were studied in Santa Catarina Island (SCI), in which 137 D. marsupialis were collected. Sixty two opossums were collected at the Arvoredo Island (AI), located 12 miles north from SCI. All captured animals were submitted to parasitological examinations that revealed the presence of T. cruzi in 21.9% of the opossums captured in SCI and 45.2% among opossums captured in the AI. The presence of P. megistus was detected in most of the D. marsupialis nests collected in the SCI, however, in the non-inhabited AI only eight triatomines were collected during the whole study. The presence of T. cruzi-infected D. marsupialis associated with P. megistus in human dwellings in the SCI, and the high infection rate of D. marsupilais by T. cruzi in the absence of a high vector density are discussed.


Subject(s)
Chagas Disease/veterinary , Opossums/parasitology , Trypanosoma cruzi/isolation & purification , Animals , Brazil/epidemiology , Chagas Disease/epidemiology , Disease Reservoirs , Female , Humans , Insect Vectors/parasitology , Male , Panstrongylus/parasitology , Prevalence
3.
Acta Trop ; 74(1): 89-93, 2000 Jan 05.
Article in English | MEDLINE | ID: mdl-10643912

ABSTRACT

Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmania-specific monoclonal antibodies (MAbs), and by PCR amplification and hybridization assay of the mini-exon gene with group specific probes. The results obtained with the MAbs were in agreement with the genetic marker. Two isolates (MHOM/BR/89/JSC89-H1 and MHOM/BR/89/JSC89-H2) were identified as L. (Leishmania) amazonensis and two (MHOM/BR/96/LSC96-H3 and MHOM/BR/97/LSC97-H4) as L. (Viannia) braziliensis. The southernmost autochthonous cases of ACL in Brazil are due to two different Leishmania sp. species, confirming the spreading of ACL on the American continent.


Subject(s)
Antibodies, Protozoan/isolation & purification , Leishmania braziliensis/isolation & purification , Leishmania mexicana/isolation & purification , Leishmaniasis, Cutaneous/parasitology , Adolescent , Adult , Animals , Antibodies, Monoclonal/isolation & purification , Brazil , Exons , Genetic Markers , Humans , Leishmania braziliensis/genetics , Leishmania braziliensis/immunology , Leishmania mexicana/genetics , Leishmania mexicana/immunology , Middle Aged , Polymerase Chain Reaction , Rural Population , Species Specificity
4.
Trop Med Int Health ; 5(12): 848-54, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11169273

ABSTRACT

During the 1950s, three foci of Wuchereria bancrofti transmission were identified in the State of Santa Catarina, Brazil. In Florianópolis, São José da Ponta Grossa and Barra da Laguna community treatment of bancroftian filariasis with diethylcarbamazine (DEC) was performed using two distinct approaches, without vector control or improvements in sanitation. In two of the three communities only microfilaraemic individuals were treated, while in Barra da Laguna the entire population received DEC treatment regardless of their infection status. In both cases, transmission of the parasite was blocked and no new cases were detected in all localities for up to 10 years. Recently, a new survey in São José da Ponta Grossa and Barra communities revealed no microfilaria-positive individuals, including residents that were positive in the 1950s. These data confirm that transmission of W. bancrofti was interrupted in Santa Catarina, and mass treatment appears to be more effective than treatment of microfilaraemic individuals only.


Subject(s)
Diethylcarbamazine/therapeutic use , Elephantiasis, Filarial/prevention & control , Filaricides/therapeutic use , Mass Screening , Preventive Health Services , Wuchereria bancrofti , Animals , Brazil/epidemiology , Elephantiasis, Filarial/epidemiology , Humans , Mass Screening/methods , Preventive Health Services/methods
6.
J Parasitol ; 84(3): 601-7, 1998 Jun.
Article in English | MEDLINE | ID: mdl-9645864

ABSTRACT

Seven Trypanosoma spp. isolates obtained from bats (Eptesicus sp.) were characterized using experimental infection in mice, triatomines, and culicines; complement lysis; indirect fluorescence assays; as well as isoenzyme and random-amplified polymorphic DNA (RAPD) profiles. The Trypanosoma sp. isolates were compared with Trypanosoma cruzi, Trypanosoma rangeli. and 2 other bat trypanosomes species, Trypanosoma vespertilionis and Trypanosoma hastatus. Trypanosoma sp. isolates were different from the other species in all experiments, except in complement lysis. Experimental infection of triatomines and culicines with Trypanosoma sp. proved to be transitory. These parasites were noninfective for both normal and immunosuppressed mice. Isoenzyme and RAPD profiles obtained for Trypanosoma sp. were quite distinct from T. cruzi and T. rangeli and closely related to T. vespertilionis and T. hastatus. No cross-reaction was observed between sera from mice infected with Trypanosoma sp. and the other trypanosomatids and vice-versa. Trypanosoma sp. induced no protection against T. cruzi infection in mice. The very low, or nonsimilarity between Trypanosoma sp. isolates and the other species used in this study suggests that they might be members of a distinct bat trypanosome species. However, further studies should be done to prove their affinities with Trypanosoma cruzi-marinkellei, another trypanosome species from bats.


Subject(s)
Aedes/parasitology , Chiroptera/parasitology , Insect Vectors/parasitology , Triatominae/parasitology , Trypanosoma/physiology , Trypanosomiasis/veterinary , Animals , Brazil , Complement System Proteins/immunology , Disease Reservoirs , Fluorescent Antibody Technique, Indirect/veterinary , Guinea Pigs , Humans , Immune Sera/immunology , Isoenzymes/analysis , Male , Mice , Random Amplified Polymorphic DNA Technique , Trypanosoma/classification , Trypanosoma/isolation & purification , Trypanosomiasis/parasitology , Trypanosomiasis/transmission
7.
Acta Trop ; 60(3): 167-77, 1995 Dec.
Article in English | MEDLINE | ID: mdl-8907395

ABSTRACT

Sixty eight Trypanosoma cruzi strains were isolated in the state of Santa Catarina, Southern Brazil, from sylvatic reservoirs or naturally infected vectors and characterized by their biological behaviour in mice, morphology of bloodstream forms and isoenzyme profiles. Twenty eight strains were isolated from the triatomine bug (Panstrongylus megistus), 2 from rodents (Echimys dasythrix and Akodon sp) and 38 from opossums (Didelphis marsupialis). The infectivity in mice of 48 T. cruzi strains showed that 2 (4.2%) were of high virulence, 19 (39.6%) of medium virulence, 15 (31.2%) of low virulence and 12 (25.0%) produced subpatent parasitemia in mice. A morphological study of bloodstream trypomastigotes from 8 T. cruzi strains showed a predominance of stout forms. The isoenzyme pattern of 59 T. cruzi strains showed that 54 (91.5%) belonged to zymodeme Z1, 3 (5.1%) to zymodeme Z2 and 2 (3.4%) to mixed zymodemes, Z1 and Z2. All 34 T. cruzi strains analyzed from opossums were Z1. Three out of 5 strains isolated from adults of P. megistus collected inside houses, belonged to zymodeme Z2 and two strains exhibited mixed zymodemes, Z1 and Z2, in 5 out of 6 enzymes studied. Although the State of Santa Catarina is a non endemic region for human Chagas'disease, the presence of zymodeme Z2 parasites in the sylvatic vector, P. megistus, captured in domiciliary environments suggests the possibility of human and/or domestic mammal infection by T. cruzi.


Subject(s)
Disease Vectors , Isoenzymes/analysis , Parasitemia/parasitology , Trypanosoma cruzi/isolation & purification , Animals , Disease Reservoirs , Male , Mice , Opossums , Trypanosoma cruzi/enzymology , Trypanosoma cruzi/pathogenicity , Virulence
10.
Mem. Inst. Oswaldo Cruz ; 90(1): 121-124, Jan.-Feb. 1995.
Article in English | LILACS | ID: lil-319920

ABSTRACT

The influence of environmental and biological factors on the efficacy of Bacillus thuringiensis serovar israelensis and B. sphaericus as mosquito larvicides are reviewed. The importance of strain dependence, cultivating media/methods, mosquito species/specificity, formulations and their relation to mosquito feeding habits, as well as temperature, solar exposure, larval density and concomitant presence of other aquatic organisms are addressed with reference to the present status of knowledge in Brazil.


Subject(s)
Animals , Bacillus thuringiensis , Culicidae , Mosquito Control , Brazil
11.
Mem Inst Oswaldo Cruz ; 90(1): 121-4, 1995.
Article in English | MEDLINE | ID: mdl-8524073

ABSTRACT

The influence of environmental and biological factors on the efficacy of Bacillus thuringiensis serovar israelensis and B. sphaericus as mosquito larvicides are reviewed. The importance of strain dependence, cultivating media/methods, mosquito species/specificity, formulations and their relation to mosquito feeding habits, as well as temperature, solar exposure, larval density and concomitant presence of other aquatic organisms are addressed with reference to the present status of knowledge in Brazil.


Subject(s)
Bacillus thuringiensis/physiology , Culicidae/microbiology , Mosquito Control/methods , Animals , Brazil
12.
Rev Inst Med Trop Sao Paulo ; 36(1): 43-50, 1994.
Article in Portuguese | MEDLINE | ID: mdl-7997773

ABSTRACT

The aim of this work was to verify the colonization of Panstrongylus megistus on artificial ecotopes in Florianópolis, in the Santa Catarina Island, South Brazil. For this purpose 443 houses of the Lagoa district and 779 house annexes (524 chicken-houses, 46 corrals and 209 storage-houses) in 9 different places were examined from 1985 to 1992. These ecotopes, which include ceilings and basements, were checked after application of dislodging liquid (Pirisa 5%). Colonization by P. megistus was verified in two houses, three chicken-houses and one storage-house of the Lagoa district, where eggs, nymphs and adults were collected. To verify local reports of P. megistus occurrence, another two houses and one school were investigated. The colonization at all of these places was confirmed. In the 9 artificial ecotopes examined, 559 eggs, 305 nymphs and 24 adults were collected. The infection rate of P. megistus by Trypanosoma cruzi was 55.3% (182/329). A similar infection rate of 56.5% (78/138) was obtained in adults of P. megistus from sylvatic ecotopes and in adults captured in the houses by the inhabitants between 1983 to 1991. Precipitin tests revealed blood from just one source in 94.0% of the insects (170/181). Human blood was found in 80.6% (25/31) of the adults and in 5.8% (1/17) of the nymphs captured in the houses. These results suggest the need to ally serious epidemiologic vigilance to the effort of the inhabitants in order to avoid the risk of domiciliation of P. megistus in the houses.


Subject(s)
Insect Vectors/parasitology , Panstrongylus/physiology , Animals , Brazil , Chagas Disease/transmission , Parasite Egg Count , Precipitin Tests
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