ABSTRACT
Secretome of primary cultures is an accessible source of biological markers compared to more complex and less decipherable mixtures such as serum or plasma. The protonation state (PS) of secretome reflects the metabolism of cells and can be used for cancer early detection. Here, we demonstrate a superhydrophobic organic electrochemical device that measures PS in a drop of secretome derived from liquid biopsies. Using data from the sensor and principal component analysis (PCA), we developed algorithms able to efficiently discriminate tumour patients from non-tumour patients. We then validated the results using mass spectrometry and biochemical analysis of samples. For the 36 patients across three independent cohorts, the method identified tumour patients with high sensitivity and identification as high as 100% (no false positives) with declared subjects at-risk, for sporadic cancer onset, by intermediate values of PS. This assay could impact on cancer risk management, individual's diagnosis and/or help clarify risk in healthy populations.
ABSTRACT
The authors report their experience in the surgical management of acute cholecystitis. From 1979 to 1991, 308 patients underwent early cholecystectomy. Results obtained, accuracy of pre- and intraoperative diagnostic investigations, and surgical strategy are discussed in relation to prognostic indexes. Personal experience and literature review indicate early cholecystectomy as the treatment of choice in acute cholecystitis.
