Characteristics of enterovirus infection associated neurologic disease associated in a pediatric population in Spain.

INTRODUCTION: Enteroviruses are a type of RNA-strained virus with more than 100 different genotypes. Infection can be asymptomatic, and, if any, symptoms can range from mild to severe. Some patients can develop neurological involvement, such as aseptic meningitis, encephalitis, or even cardiorespiratory failure. However, in children, the risk factors for developing severe neurological involvement are not well understood. The aim of this retrospective study was to analyze some characteristics associated with severe neurological involvement in children hospitalized for neurological disease after enterovirus infection. METHODS: retrospective observational study analyzing clinical, microbiological and radiological data of 174 children hospitalized from 2009 to 2019 in our hospital. Patients were classified according to the World Health Organization case definition for neurological complications in hand, foot and mouth disease. RESULTS: Our findings showed that, in children between 6 months old and 2 years of age, the appearance of neurological symptoms within the first 12h from infection onset-especially if associated with skin rash-was a significant risk factor for severe neurological involvement. Detection of enterovirus in cerebrospinal fluid was more likely in patients with aseptic meningitis. By contrast, other biological samples (e.g., feces or nasopharyngeal fluids) were necessary to detect enterovirus in patients with encephalitis. The genotype most commonly associated with the most severe neurological conditions was EV-A71. E-30 was mostly associated with aseptic meningitis. CONCLUSIONS: Awareness of the risk factors associated with worse neurological outcomes could help clinicians to better manage these patients to avoid unnecessary admissions and/or ancillary tests.

High-resolution spectral information enables phenotyping of leaf epicuticular wax in wheat.

BACKGROUND: Epicuticular wax (EW) is the first line of defense in plants for protection against biotic and abiotic factors in the environment. In wheat, EW is associated with resilience to heat and drought stress, however, the current limitations on phenotyping EW restrict the integration of this secondary trait into wheat breeding pipelines. In this study we evaluated the use of light reflectance as a proxy for EW load and developed an efficient indirect method for the selection of genotypes with high EW density. RESULTS: Cuticular waxes affect the light that is reflected, absorbed and transmitted by plants. The narrow spectral regions statistically associated with EW overlap with bands linked to photosynthetic radiation (500 nm), carotenoid absorbance (400 nm) and water content (~ 900 nm) in plants. The narrow spectral indices developed predicted 65% (EWI-13) and 44% (EWI-1) of the variation in this trait utilizing single-leaf reflectance. However, the normalized difference indices EWI-4 and EWI-9 improved the phenotyping efficiency with canopy reflectance across all field experimental trials. Indirect selection for EW with EWI-4 and EWI-9 led to a selection efficiency of 70% compared to phenotyping with the chemical method. The regression model EWM-7 integrated eight narrow wavelengths and accurately predicted 71% of the variation in the EW load (mg·dm-2) with leaf reflectance, but under field conditions, a single-wavelength model consistently estimated EW with an average RMSE of 1.24 mg·dm-2 utilizing ground and aerial canopy reflectance. CONCLUSIONS: Overall, the indices EWI-1, EWI-13 and the model EWM-7 are reliable tools for indirect selection for EW based on leaf reflectance, and the indices EWI-4, EWI-9 and the model EWM-1 are reliable for selection based on canopy reflectance. However, further research is needed to define how the background effects and geometry of the canopy impact the accuracy of these phenotyping methods.

Fluorescence angiography with indocyanine green (ICG) to evaluate anastomosis in colorectal surgery: where does it have more value?

BACKGROUND: Indocyanine green (ICG) fluorescence angiography (FA) was introduced to provide the real-time intraoperative evaluation of the vascular supply of anastomosis. However, further studies are required to evaluate its advantages in colorectal surgery and to know in which procedure this technology has more value. The aim of the present study is to assess the usefulness of the ICG-FA in the colorectal anastomosis evaluation and to evaluate where it is most useful depending on type of resection performed in terms of change of section line based on the ICG-FA and anastomotic leakage (AL) rates. METHODS: This is a prospective study. From September 2014 to November 2018, all patients who underwent any colorectal surgical procedure with ICG-FA in our center were enrolled in the study. Based on the type of surgery, patients were grouped in 4 categories: Group A, right hemicolectomy; Group B, segmental resection of the splenic flexure; Group C, left hemicolectomy; and Group D, anterior resection of the rectum. RESULTS: One-hundred-ninety-two unselected consecutive patients were enrolled: 67 in group A, 9 in B, 81 in C, and 35 in D. Change of section line based on ICG-FA occurred in 35 cases (18.2%): 4 in group A (6%), 1 in group B (11.1%), 21 in group C (25.9%), and 9 in group D (25.7%). ALs occurred in 5 patients (2.6%): 2 in group A (3%), 1 in C (1.2%), and 2 in D (5.7%). CONCLUSIONS: ICG-FA leads to significantly more changes in the resection line in case of left hemicolectomy followed by anterior resection. FA is a promising optical imaging technique to reduce the AL incidence after colorectal procedures. To confirm this data, further studies with wider sample size and with an objective evaluation of the anastomotic perfusion are required.

Hybrid Wheat Prediction Using Genomic, Pedigree, and Environmental Covariables Interaction Models.

In this study, we used genotype × environment interactions (G×E) models for hybrid prediction, where similarity between lines was assessed by pedigree and molecular markers, and similarity between environments was accounted for by environmental covariables. We use five genomic and pedigree models (M1-M5) under four cross-validation (CV) schemes: prediction of hybrids when the training set (i) includes hybrids of all males and females evaluated only in some environments (T2FM), (ii) excludes all progenies from a randomly selected male (T1M), (iii) includes all progenies from 20% randomly selected females in combination with all males (T1F), and (iv) includes one randomly selected male plus 40% randomly selected females that were crossed with it (T0FM). Models were tested on a total of 1888 wheat ( L.) hybrids including 18 males and 667 females in three consecutive years. For grain yield, the most complex model (M5) under T2FM had slightly higher prediction accuracy than the less complex model. For T1F, the prediction accuracy of hybrids for grain yield and other traits of the most complete model was 0.50 to 0.55. For T1M, Model M3 exhibited high prediction accuracies for flowering traits (0.71), whereas the more complex model (M5) demonstrated high accuracy for grain yield (0.5). For T0FM, the prediction accuracy for grain yield of Model M5 was 0.61. Including genomic and pedigree gave relatively high prediction accuracy even when both parents were untested. Results show that it is possible to predict unobserved hybrids when modeling genomic general combining ability (GCA) and specific combining ability (SCA) and their interactions with environments.

Amoebic toxic colitis: analysis of factors related to mortality.

BACKGROUND: Toxic or fulminant colitis due to Entamoeba histolytica infrequently presents but is very serious. Unfortunately, there are numerous contradictory factors related to mortality. METHODS: We analyzed several cases of E. histolytica infection to determine the factors related to mortality. We included patients >15 years of age who were histopathologically diagnosed with amoebic toxic colitis and treated from January 2000 through December 2006. We evaluated demographic, clinical, laboratorial, surgical, and histopathological characteristics. RESULTS: We examined 24 patients and recorded 12 deaths (50%). Twenty patients underwent surgery within a mean time of 24 hours (range: 8-120 hours). Tenesmus and intestinal perforation were determined to be statistically significant (P<0·05) by univariate analysis. Three models of logistic regression were able to determine three statistically significant factors that affected mortality: (1) tenesmus and a lymphocyte count <1·5×10(3) cell/µl; 2) depth of invasion beyond the mucosa and a lymphocyte count <1·5×10(3) cell/µl; 3) time spent with symptoms and perforation. CONCLUSIONS: The mortality rate determined in this study is similar to previously reported series. A low lymphocyte count, significant depth of invasion, and intestinal perforation were determined to be the factors related to increased mortality, while tenesmus and limited amount of time spent with symptoms were associated with survival. Quick diagnosis and appropriate treatment are important factors that reduce mortality.

Primary closure in colon trauma.

BACKGROUND: Primary repair of colon injuries is an accepted therapeutic option; however, controversy persists regarding its safety. Our objective was to report the evolution and presence of complications in patients with colon injury who underwent primary closure and to determine if the time interval (>6 h), degree of injury, contamination, anatomic site injured, PATI (Penetrating Abdominal Trauma Index) >25, and the presence of other injuries in colon trauma are associated with increased morbidity and mortality. METHODS: This was a prospective, observational, longitudinal and descriptive study conducted at the Central Hospital "Dr. Ignacio Morones Prieto," San Luis Potosí, Mexico, from January 1, 2003 to December 31, 2007. We included patients with abdominal trauma with colon injury subjected to surgical treatment. chi(2) was used for basic statistical analysis. RESULTS: There were 481 patients with abdominal trauma who underwent surgery; 77(16.1%) had colon injury. Ninety percent (n = 69) were treated in the first 6 h; 91% (n = 70) were due to penetrating injuries, and gunshot wound accounted for 48% (n = 37). Transverse colon was the most frequently injured (38%) (n = 29). Grade I and II injuries accounted for 75.3% (n = 58). Procedures included primary repair (76.66 %) (n = 46); resection with anastomosis (8.3%) (n = 5); and colostomy (15%) (n = 9). Associated injuries were present in 76.6% (n = 59). There was some degree of contamination in 85.7% (n = 66); 82.8% (58) had PATI <25. Complications associated with the surgical procedure were observed in 28.57% (n = 22). Reoperation was necessary in 10% (n = 8). Average hospital stay was 11.4 days. Mortality was 3.8% (n = 3); none of these were related to colon injury. CONCLUSIONS: Primary repair is a safe procedure for treatment of colon injuries. Patients with primary repair had lower morbidity (p <0.009). Surgery during the first 6 h (p <0.006) and in hemodynamically stable patients (p <0.014) had a lower risk of complications.

Cierre primario en trauma de colon / Primary closure in colon trauma

Objetivo: Informar si la evolución > 6 horas, grado de contaminación y lesión, sitio anatómico lesionado, PATI (penetrating abdominal trauma index) > 25 y presencia de otras lesiones en trauma de colon, se asocian a mayor morbimortalidad en pacientes con lesión colónica a quienes se les realizó cierre primario. Material y métodos: Estudio prospectivo, observacional, longitudinal, descriptivo, en el Hospital Central “Dr. Ignacio Morones Prieto”, San Luis Potosí. Se incluyeron pacientes con trauma abdominal sometidos a cirugía que presentaron lesión colónica. Análisis estadístico básico con χ2. Resultados: 481 pacientes fueron intervenidos por trauma abdominal; 77 (16.1 %) tuvieron lesión colónica, de los cuales 90 % (n = 69) se intervino en las primeras seis horas; 91 % fue lesión penetrante. El colon transverso fue el más lesionado (38 %, n = 29); las lesiones grado I y II representaron 75.3 % (n = 58). Se efectuó cierre primario en 76.66 % (n = 46), resección con anastomosis en 8.3 % (n = 5) y colostomía en 15 % (n = 9). Hubo lesiones asociadas en 76.6 % (n = 59) y contaminación en 85.7 % (n = 66); 82.8 % (58) tuvo PATI < 25; complicaciones asociadas al procedimiento operatorio, 28.57 % (n = 22); reintervenciones, 10 % (n = 8); estancia hospitalaria promedio, 11.4 días; mortalidad no relacionada a lesión de colon, 3.8 % (n = 3). Conclusiones: El cierre primario es un procedimiento seguro para el tratamiento de lesiones colónicas. Los pacientes con cierre primario presentaron menor morbilidad (p < 0.009). Los pacientes con cirugía en las primeras seis horas (p < 0.006) y estabilidad hemodinámica (p < 0.014) tuvieron menor riesgo de complicación.

BACKGROUND: Primary repair of colon injuries is an accepted therapeutic option; however, controversy persists regarding its safety. Our objective was to report the evolution and presence of complications in patients with colon injury who underwent primary closure and to determine if the time interval (>6 h), degree of injury, contamination, anatomic site injured, PATI (Penetrating Abdominal Trauma Index) >25, and the presence of other injuries in colon trauma are associated with increased morbidity and mortality. METHODS: This was a prospective, observational, longitudinal and descriptive study conducted at the Central Hospital "Dr. Ignacio Morones Prieto," San Luis Potosí, Mexico, from January 1, 2003 to December 31, 2007. We included patients with abdominal trauma with colon injury subjected to surgical treatment. chi(2) was used for basic statistical analysis. RESULTS: There were 481 patients with abdominal trauma who underwent surgery; 77(16.1%) had colon injury. Ninety percent (n = 69) were treated in the first 6 h; 91% (n = 70) were due to penetrating injuries, and gunshot wound accounted for 48% (n = 37). Transverse colon was the most frequently injured (38%) (n = 29). Grade I and II injuries accounted for 75.3% (n = 58). Procedures included primary repair (76.66 %) (n = 46); resection with anastomosis (8.3%) (n = 5); and colostomy (15%) (n = 9). Associated injuries were present in 76.6% (n = 59). There was some degree of contamination in 85.7% (n = 66); 82.8% (58) had PATI <25. Complications associated with the surgical procedure were observed in 28.57% (n = 22). Reoperation was necessary in 10% (n = 8). Average hospital stay was 11.4 days. Mortality was 3.8% (n = 3); none of these were related to colon injury. CONCLUSIONS: Primary repair is a safe procedure for treatment of colon injuries. Patients with primary repair had lower morbidity (p <0.009). Surgery during the first 6 h (p <0.006) and in hemodynamically stable patients (p <0.014) had a lower risk of complications.

Neumotórax espontáneo en el embarazo: presentación de un caso / Spontaneous pneumothorax in pregnancy: case report

Introducción: el neumotórax espontáneo es poco frecuente durante el embarazo. La causa más común es la ruptura de una bula o burbuja subpleural apical a causa de incremento en la demanda respiratoria durante el periodo periparto. El principal riesgo materno es compromiso respiratorio; los riesgos fetales, reducción en el aporte de oxígeno y trabajo de parto pretérmino. El riesgo de recurrencia es de 30 a 40 %, particularmente durante el trabajo de parto. El tratamiento se basa en la magnitud del neumotórax; hasta 75 % de los casos se trata con pleurostomía cerrada. Caso clínico: mujer de 22 años de edad, segundo embarazo intrauterino, de 24.2 semanas de gestación, sin antecedente de tabaquismo ni otras toxicomanías, sin sintomatología respiratoria ni historia de neumotórax previo. Presentó dolor pleurítico en hemitórax derecho de inicio súbito, transfictivo y pungitivo, acompañado de disnea. A la exploración física, síndrome de rarefacción pulmonar, sin compromiso obstétrico. La radiografía simple de tórax en proyección posteroanterior mostró neumotórax derecho con colapso pulmonar total. Conclusiones: debe considerarse el diagnóstico en casos de dolor torácico y disnea durante el embarazo y trabajo de parto. El caso reseñado se manejó conservadoramente de manera exitosa con drenaje intercostal a mediano plazo, sin recurrencia ni complicaciones.

BACKGROUND: Spontaneous pneumothorax is a rare condition during pregnancy. The most common cause is the rupture of a subpleural apical bulla or bleb, due to the increased respiratory demand of the peripartum period. The main risk for the mother is respiratory compromise; fetal risks include reduction in oxygen supply and preterm labor. The risk of recurrence is 30-40%, particularly during labor. Treatment is based on the magnitude of pneumothorax. Up to 75% of patients are treated with chest tube drainage. We present the case report of a previously healthy patient. CASE REPORT: Our patient was a 22-year-old female at 24.2 weeks of her second pregnancy. The patient was a non-smoker, had no history of any drug addictions, and no history of previous pulmonary disease. The patient presented with sudden onset of pleuritic right-sided pleuritic chest pain associated with dyspnea. Chest examination was notable for decreased breath sounds and hyperresonance over the right hemithorax. Chest radiography showed right spontaneous pneumothorax with total lung collapse. CONCLUSIONS: Diagnosis of pneumothorax should be considered in any pregnant woman with chest pain and dyspnea. The presented case was successfully treated with closed intercostal chest tube thoracostomy for 7 weeks.

Women in developing countries and benefit sharing.

The aim of this paper is to show that any process of benefit sharing that does not guarantee the representation and participation of women in the decision-making process, as well as in the distribution of benefits, contravenes a central demand of social justice. It is argued that women, particularly in developing countries, can be excluded from benefits derived from genetic research because of existing social structures that promote and maintain discrimination. The paper describes how the structural problem of gender-based inequity can impact on benefit sharing processes. At the same time, examples are given of poor women's ability to organise themselves and to achieve social benefits for entire communities. Relevant international guidelines (e.g. the Convention on Biodiversity) recognise the importance of women's contributions to the protection of biodiversity and thereby, implicitly, their right to a share of the benefits, but no mechanism is outlined on how to bring this about. The authors make a clear recommendation to ensure women's participation in benefit sharing negotiations by demanding seats at the negotiation table.

[Spontaneous pneumothorax in pregnancy. Case report]. / Neumotórax espontáneo en el embarazo. Presentación de un caso.

BACKGROUND: Spontaneous pneumothorax is a rare condition during pregnancy. The most common cause is the rupture of a subpleural apical bulla or bleb, due to the increased respiratory demand of the peripartum period. The main risk for the mother is respiratory compromise; fetal risks include reduction in oxygen supply and preterm labor. The risk of recurrence is 30-40%, particularly during labor. Treatment is based on the magnitude of pneumothorax. Up to 75% of patients are treated with chest tube drainage. We present the case report of a previously healthy patient. CASE REPORT: Our patient was a 22-year-old female at 24.2 weeks of her second pregnancy. The patient was a non-smoker, had no history of any drug addictions, and no history of previous pulmonary disease. The patient presented with sudden onset of pleuritic right-sided pleuritic chest pain associated with dyspnea. Chest examination was notable for decreased breath sounds and hyperresonance over the right hemithorax. Chest radiography showed right spontaneous pneumothorax with total lung collapse. CONCLUSIONS: Diagnosis of pneumothorax should be considered in any pregnant woman with chest pain and dyspnea. The presented case was successfully treated with closed intercostal chest tube thoracostomy for 7 weeks.

Limiting factors impacting on voluntary first person informed consent in the Philippines.

How well can institutional guidelines help ensure the dignity, rights, safety and well being of research participants in an underdeveloped country? In this paper I describe the limits of informed consent as an instrument for the protection of participants in the context of the Philippines. I bring to this paper my experiences as an advocate of rights, a member of an ethics review board, a researcher on the ethics of research and as an observer of the dynamics of clinical practice in an academic public teaching hospital of the University of the Philippines where I am professor.

Neumotorax (D) en RN / Neumotorax (D) in RN

Presenta Proceso de enfermería basado en un estudio realizado a un bebé ingresado en la sala de Neonatología en la Unidad de Cuidados Intensivos, del Hospital San Juan de Dios, durante el período de mayo a junio del 2001, con frecuencia cardíaca, sianótico. El objetivo del proceso es aplicar las etapas del proceso de enfermería en la elaboración de acciones que ayuden a mejorar las condiciones del paciente con Neumotorax

Sevoflurane a flujos bajos en anestesia general para niños: Hospital Escuela Oscar Danilo Rosales-León

El presente estudio es de tipo descriptivo y de corte transversal realizado en Hospital Escuela Oscar Danilo Rosales en la ciudad de León en el período 1999 a febrero del 2000. Se realizó con un total de 25 pacientes programados para cirugía electiva con sevoflurano en flujos con anestesia general. S0e utilizó una ficha de recolección de datos para cada pacientes estudiado. El tiempo promedio de inducción hasta el plano quirúrgico fue de 6 a 10 minutos en todas las edades para un total de 14 casos con un 56 porciento. Según los grupos etáreos la concentración alveolar mínimo utilizados fue de 4-6 porciento es el 48 prociento de los pacientes estudiados de un 8 porciento en niños menores de 6 años. Con CAM de 2-3 porciento debido a la administración de fármacos con premedicación y mayor habilidad en estas edades. Se observa ligero aumento de la frecuencia cardíaca al momento de la inducción debido a la ansiedad y el temor, se utilizó flujos de 2.5 litros en 16 pacientes para un 64 porciento y en un 36 porciento de los casos de flujos de 2 litros, no encontrando en ambos grupos diferenciados significativos que pudieran seguir estados de hipoxia transitoria. En niños menores de 3 años predominó la anestesia inhalatoria para un 20 porciento de los casos, a partir de los 4 años se asoció el Pentotal al Fentanil en 12 casos para un 48 porciento, observándose una buena estabilidad durante el transquirúrgico, igual sucede cuando se usa otros tipos de agentes inhalatorios durante la anestesia general en estas edades. Se observó complicaciones como bradicardia, hipoxia transitoria, agitación larngoespasmo en 5 pacientes que corresponde al 25 porciento. Esto se debió a una anestesia superficial, pues no se alcanzaron concentraciones alveolares y cerebrales adecuadas por el poco volumen de anestésico dentro del vaporizador a causa de su elevado costo y que en nuestro hosptal no contamos con este fármaco