CRISPRals: A Web Database for Assessing the CRISPR Defense System in the Ralstonia solanacearum Species Complex to Avoid Phage Resistance.

Clustered regularly interspaced short palindromic repeats (CRISPR) has been widely characterized as a defense system against phages and other invading elements in bacteria and archaea. A low percentage of Ralstonia solanacearum species complex (RSSC) strains possess the CRISPR array and the CRISPR-associated proteins (Cas) that would confer immunity against various phages. To provide a wide-range screen of the CRISPR presence in the RSSC, we analyzed 378 genomes of RSSC strains to find the CRISPR locus. We found that 20.1, 14.3, and 54.5% of the R. solanacearum, R. pseudosolanacearum, and R. syzygii strains, respectively, possess the CRISPR locus. In addition, we performed further analysis to identify the respective phages that are restricted by the CRISPR arrays. We found 252 different phages infecting different strains of the RSSC, by means of the identification of similarities between the protospacers in phages and spacers in bacteria. We compiled this information in a database with web access called CRISPRals (https://crisprals.yachaytech.edu.ec/). Additionally, we made available a number of tools to detect and identify CRISPR array and Cas genes in genomic sequences that could be uploaded by users. Finally, a matching tool to relate bacteria spacer with phage protospacer sequences is available. CRISPRals is a valuable resource for the scientific community that contributes to the study of bacteria-phage interaction and a starting point that will help to design efficient phage therapy strategies.

Diversity and evolutionary dynamics of spore-coat proteins in spore-forming species of Bacillales.

Among members of the Bacillales order, there are several species capable of forming a structure called an endospore. Endospores enable bacteria to survive under unfavourable growth conditions and germinate when environmental conditions are favourable again. Spore-coat proteins are found in a multilayered proteinaceous structure encasing the spore core and the cortex. They are involved in coat assembly, cortex synthesis and germination. Here, we aimed to determine the diversity and evolutionary processes that have influenced spore-coat genes in various spore-forming species of Bacillales using an in silico approach. For this, we used sequence similarity searching algorithms to determine the diversity of coat genes across 161 genomes of Bacillales. The results suggest that among Bacillales, there is a well-conserved core genome, composed mainly by morphogenetic coat proteins and spore-coat proteins involved in germination. However, some spore-coat proteins are taxa-specific. The best-conserved genes among different species may promote adaptation to changeable environmental conditions. Because most of the Bacillus species harbour complete or almost complete sets of spore-coat genes, we focused on this genus in greater depth. Phylogenetic reconstruction revealed eight monophyletic groups in the Bacillus genus, of which three are newly discovered. We estimated the selection pressures acting over spore-coat genes in these monophyletic groups using classical and modern approaches and detected horizontal gene transfer (HGT) events, which have been further confirmed by scanning the genomes to find traces of insertion sequences. Although most of the genes are under purifying selection, there are several cases with individual sites evolving under positive selection. Finally, the HGT results confirm that sporulation is an ancestral feature in Bacillus.

Diversity and Evolutionary Dynamics of Antiphage Defense Systems in Ralstonia solanacearum Species Complex.

Over the years, many researchers have reported a great diversity of bacteriophages infecting members of the Ralstonia solanacearum species complex (RSSC). This diversity has driven bacterial evolution by leading the emergence and maintenance of bacterial defense systems to combat phage infection. In this work, we present an in silico study of the arsenal of defense systems that RSSC harbors and their evolutionary history. For this purpose, we used a combination of genomic, phylogenetic and associative methods. We found that in addition to the CRISPR-Cas system already reported, there are eight other antiphage defense systems including the well-known Restriction-Modification and Toxin-Antitoxin systems. Furthermore, we found a tenth defense system, which is dedicated to reducing the incidence of plasmid transformation in bacteria. We undertook an analysis of the gene gain and loss patterns of the defense systems in 15 genomes of RSSC. Results indicate that the dynamics are inclined toward the gain of defense genes as opposed to the rest of the genes that were preferably lost throughout evolution. This was confirmed by evidence on independent gene acquisition that has occurred by profuse horizontal transfer. The mutation and recombination rates were calculated as a proxy of evolutionary rates. Again, genes encoding the defense systems follow different rates of evolution respect to the rest of the genes. These results lead us to conclude that the evolution of RSSC defense systems is highly dynamic and responds to a different evolutionary regime than the rest of the genes in the genomes of RSSC.

A genome-wide scan for genes under balancing selection in the plant pathogen Ralstonia solanacearum.

BACKGROUND: Plant pathogens are under significant selective pressure by the plant host. Consequently, they are expected to have adapted to this condition or contribute to evading plant defenses. In order to acquire long-term fitness, plant bacterial pathogens are usually forced to maintain advantageous genetic diversity in populations. This strategy ensures that different alleles in the pathogen's gene pool are maintained in a population at frequencies larger than expected under neutral evolution. This selective process, known as balancing selection, is the subject of this work in the context of a common bacterial phytopathogen. We performed a genome-wide scan of Ralstonia solanacearum species complex, an aggressive plant bacterial pathogen that shows broad host range and causes a devastating disease called 'bacterial wilt'. RESULTS: Using a sliding window approach, we analyzed 57 genomes from three phylotypes of the R. solanacearum species complex to detect signatures of balancing selection. A total of 161 windows showed extreme values in three summary statistics of population genetics: Tajima's D, θw and Fu & Li's D*. We discarded any confounding effects due to demographic events by means of coalescent simulations of genetic data. The prospective windows correspond to 78 genes with known function that map in any of the two main replicons (1.7% of total number of genes). The candidate genes under balancing selection are related to primary metabolism and other basal activities (51.3%) or directly associated to virulence (48.7%), the latter being involved in key functions targeted to dismantle plant defenses or to participate in critical stages in the pathogenic process. CONCLUSIONS: We identified various genes under balancing selection that play a significant role in basic metabolism as well as in virulence of the R. solanacearum species complex. These genes are useful to understand and monitor the evolution of bacterial pathogen populations and emerge as potential candidates for future treatments to induce specific plant immune responses.

Ultrastructural analysis of spores from diverse Bacillales species isolated from Brazilian soil.

Many species in the order Bacillales form a specialized cell type called a spore that is resistant to a range of environmental stresses. Transmission electron microscopy (TEM) reveals that the spore is comprised of a series of concentric shells, surrounding an interior compartment harbouring the spore DNA. The outermost of these shells varies considerably in morphology among species, likely reflecting adaptations to the highly diverse niches in which spores are found. To better characterize the variation in spore ultrastructure among diverse species, we used TEM to analyse spores from a collection of 23 aerobic spore-forming bacteria from the Solo do Distrito Federal (SDF strains), spanning the genera Bacillus, Lysinibacillus, Paenibacillus and Brevibacillus, isolated from soil from central Brazil. We found that the structures of these spores varied widely, as expected. Interestingly, even though these isolates are novel strains of each species, they were structurally very similar to the known examples of each species in the literature. Because in most cases, the species we analysed are poorly characterized, our data provide important evidence regarding which structural features are likely to be constant within a taxon and which are likely to vary.

Influence of Light on Plant-Phyllosphere Interaction.

Plant-phyllosphere interactions depend on microbial diversity, the plant host and environmental factors. Light is perceived by plants and by microorganisms and is used as a cue for their interaction. Photoreceptors respond to narrow-bandwidth wavelengths and activate specific internal responses. Light-induced plant responses include changes in hormonal levels, production of secondary metabolites, and release of volatile compounds, which ultimately influence plant-phyllosphere interactions. On the other hand, microorganisms contribute making some essential elements (N, P, and Fe) biologically available for plants and producing growth regulators that promote plant growth and fitness. Therefore, light directly or indirectly influences plant-microbe interactions. The usage of light-emitting diodes in plant growth facilities is helping increasing knowledge in the field. This progress will help define light recipes to optimize outputs on plant-phyllosphere communications. This review describes research advancements on light-regulated plant-phyllosphere interactions. The effects of full light spectra and narrow bandwidth-wavelengths from UV to far-red light are discussed.

Relações entre suporte social, autorregulação e consumo de outras substâncias em adultos portugueses

Neste estudo, investigaram-se as relações entre suporte social percebido, auto-regulação e comportamentos de consumo de álcool, tabaco e outras substâncias psicoactivas. A amostra foi constituída por 340 participantes. O suporte social correlacionou-se favoravelmente com a auto-regulação, mas somente na vertente de satisfação com o apoio social é que se observou esta tendência de forma evidente. O número de figuras de apoio diferenciou-se significativamente com o uso de tabaco, o que sugere o âmbito afiliativo de algumas substâncias psicoactivas. Nesse sentido, o uso de tabaco pode estar associado a uma rede social mais ampla, mas não com a qualidade do suporte social.

In this study the relationships between perceived social support, self-regulation and consuming behaviors of tobacco and other psychoactive substances we investigated. The sample consisted of 340 adult participants. Social support correlated positively with self-regulation, but was solely in the aspect of satisfaction with social support that this trend was noted clearly. The number of support providers differed considerably with the use of tobacco, which suggests the affiliative scope of some psychoactive substances. Accordingly, the use of tobacco may be associated with a larger social network, but not with the quality of social support.

Distribución espacial y estructura morfométrica de las praderas de Thalassia testudinum (Hydrocharitaceae) en dos arrecifes del Parque Nacional Sistema Arrecifal Veracruzano, México / Spatial distribution and ecological structure of Thalassia testudinum (Hydrocharitaceae) in two reefs of Veracruz Reef System National Park, Mexico

ResumenLos pastos marinos son ambientes costeros que se han visto amenazados por el incremento de las actividades humanas. Alterando de forma negativa los procesos y servicios ambientales que presentan, así como la disminución de praderas. El objetivo es generar conocimiento de la distribución, estado de la estructura y nivel de fragmentación en dos arrecifes del Parque Nacional Sistema Arrecifal Veracruzano (PNSAV). Se eligieron dos praderas en arrecifes con características distintas: Sacrificios en el Norte que se encuentra cerca de la costa y Cabezo en el Sur que está alejado de la costa. Se determinaron las características específicas de haz y área de las praderas de macrófitas sumergidas presentes e identificaron cuatro grupos de cobertura morfofuncionales. Se comprobaron diferencias significativas entre coberturas (ANOVA no paramétrico, prueba de Kruskal-Wallis). Se realizó una clasificación supervisada de una imagen espacial de alta resolución verificada con datos de campo (55 Sacrificios y 290 Cabezo). El nivel de fragmentación se calculó usando métricas de paisaje a nivel de clase y se realizaron mapas temáticos en función de las cuatro coberturas. Las praderas se encuentran dominadas por Thalassia testudinum; se tuvieron densidades máximas de 208 haces/m2 para Cabezo y 176 haces/m2 en Sacrificios. Cabezo presentó pastos de hojas cortas (9 cm) y delgadas (0.55 cm) en promedio; Sacrificios tuvo hojas más largas (23.5 cm) y gruesas (1 cm). Sacrificios mostró menor grado de fragmentación que Cabezo; en ambos casos la fragmentación de la cobertura vegetal corresponde a menos del 50 %. Aunque el arrecife Cabezo presenta una mayor fragmentación, que crea un gran número de microambientes, siendo reconocido por su importancia como zona de reclutamiento. Este trabajo sirve como una línea de base para la creación de un plan de manejo adecuado (formación de una zona núcleo de Cabezo). Es necesario complementar este trabajo con nuevos esfuerzos del reconocimiento de las praderas de pastos marinos en todos los arrecifes del PNSAV, así como de monitoreos periódicos y reconocimiento de sus servicios ecosistémicos.

AbstractSeagrasses in coastal environments have been threatened by increased human activities; these have negatively altered processes and environmental services, and have decreased grassland areas. The aim of this study was to generate knowledge of Thalassia testudinum distribution, state of the structure and fragmentation level in two reefs of the Veracruz Reef System National Park (PNSAV). Two different reefs were selected: Sacrificios in the North and near the coast, and Cabezo in the South and away from the coast. Shoot-specific and area-specific characteristics of submerged macrophytes meadows present were determined, and four morpho-functional groups were identified. Significant differences between plant coverage were tested through nonparametric ANOVA, Kruskal-Wallis test. A supervised classification of spatial high-resolution image verified with field data was performed (55 Sacrificios and 290 Cabezo). The fragmentation level was calculated using landscape metrics, class level and thematic maps were made based on four covers. The meadows were dominated by Thalassia testudinum; maximum densities were 208 shoot/m2 in Cabezo, and 176 shoot/m2 in Sacrificios. Cabezo presented grasses with short (9 cm) and thin leaves (0.55 cm) on average; while Sacrificios showed longer (23.5 cm) and thicker (1 cm) leaves. Sacrificios showed lower fragmentation degree than Cabezo; in both cases, the vegetation cover fragmentation corresponded to less than 50 %. Although Cabezo reef presents further fragmentation, which creates a large number of microenvironments, being recognized for its importance as recruitment area. This work serves as a baseline for the creation of an adequate management plan (formation of a core area of Cabezo). It is necessary to complement this work with new efforts for the recognition of seagrass prairies in all PNSAV reefs, as well as periodic monitoring and recognition of ecosystem services. Rev. Biol. Trop. 64 (2): 427-448. Epub 2016 June 01.

[Spatial distribution and ecological structure of Thalassia testudinum (Hydrocharitaceae) in two reefs of Veracruz Reef System National Park, Mexico]. / Distribución espacial y estructura morfométrica de las praderas de Thalassia testudinum (Hydrocharitaceae) en dos arrecifes del Parque Nacional Sistema Arrecifal Veracruzano, México.

Seagrasses in coastal environments have been threatened by increased human activities; these have negatively altered processes and environmental services, and have decreased grassland areas. The aim of this study was to generate knowledge of Thalassia testudinum distribution, state of the structure and fragmentation level in two reefs of the Veracruz Reef System National Park (PNSAV). Two different reefs were selected: Sacrificios in the North and near the coast, and Cabezo in the South and away from the coast. Shoot-specific and area-specific characteristics of submerged macrophytes meadows present were determined, and four morpho-functional groups were identified. Significant differences between plant coverage were tested through nonparametric ANOVA, Kruskal-Wallis test. A supervised classification of spatial high-resolution image verified with field data was performed (55 Sacrificios and 290 Cabezo). The fragmentation level was calculated using landscape metrics, class level and thematic maps were made based on four covers. The meadows were dominated by Thalassia testudinum; maximum densities were 208 shoot/m2 in Cabezo, and 176 shoot/m2 in Sacrificios. Cabezo presented grasses with short (9 cm) and thin leaves (0.55 cm) on average; while Sacrificios showed longer (23.5 cm) and thicker (1 cm) leaves. Sacrificios showed lower fragmentation degree than Cabezo; in both cases, the vegetation cover fragmentation corresponded to less than 50 %. Although Cabezo reef presents further fragmentation, which creates a large number of microenvironments, being recognized for its importance as recruitment area. This work serves as a baseline for the creation of an adequate management plan (formation of a core area of Cabezo). It is necessary to complement this work with new efforts for the recognition of seagrass prairies in all PNSAV reefs, as well as periodic monitoring and recognition of ecosystem services. .

Genome-Enabled Phylogeographic Investigation of the Quarantine Pathogen Ralstonia solanacearum Race 3 Biovar 2 and Screening for Sources of Resistance Against Its Core Effectors.

Phylogeographic studies inform about routes of pathogen dissemination and are instrumental for improving import/export controls. Genomes of 17 isolates of the bacterial wilt and potato brown rot pathogen Ralstonia solanacearum race 3 biovar 2 (R3bv2), a Select Agent in the United States, were thus analyzed to get insight into the phylogeography of this pathogen. Thirteen of fourteen isolates from Europe, Africa, and Asia were found to belong to a single clonal lineage while isolates from South America were genetically diverse and tended to carry ancestral alleles at the analyzed genomic loci consistent with a South American origin of R3bv2. The R3bv2 isolates share a core repertoire of 31 type III-secreted effector genes representing excellent candidates to be targeted with resistance genes in breeding programs to develop durable disease resistance. Toward this goal, 27 R3bv2 effectors were tested in eggplant, tomato, pepper, tobacco, and lettuce for induction of a hypersensitive-like response indicative of recognition by cognate resistance receptors. Fifteen effectors, eight of them core effectors, triggered a response in one or more plant species. These genotypes may harbor resistance genes that could be identified and mapped, cloned, and expressed in tomato or potato, for which sources of genetic resistance to R3bv2 are extremely limited.

Effectiveness of two prevention programs on alcohol use as a function of provider type / Eficacia de dos programas de prevención del consumo de alcohol en función del tipo de aplicador

Currently, it is still unclear how to translate effectively programs validated in research for use in real-world contexts. Among the efforts being made to identify strategies which optimize the application of these programs in everyday practice are tests of the differential effectiveness of the programs depending on the application agent. Method: this study analyses the effects of two programs on alcohol use and its variables as a function of provider type. Two hundred students from the first year of secondary education were distributed among five experimental conditions: four treatment conditions, in which the two programs were applied by teachers at the school or external psychologists, and a control condition. Results: the results suggest that, for both programs, teachers obtain better outcomes in alcohol use and concern about addiction. Conclusions: it is concluded that teachers are the ideal application agents in terms of efficiency, and we discuss the implications for research and preventive practice.

En la actualidad no es claro cómo traducir efectivamente los programas validados en la investigación para su aplicación en contextos reales. Entre los esfuerzos que se han realizado está el de identificar estrategias que optimicen la aplicación de estos programas en la práctica cotidiana que tienen diferente efectividad dependiendo del agente que los aplique. Metodología: este estudio analiza los efectos de dos programas sobre el consumo de alcohol y sus variables en función del aplicador. Doscientos estudiantes de primer año de secundaria se distribuyeron en cinco condiciones experimentales: cuatro en condición de tratamiento, en que se aplicaron dos programas aplicados por profesores de la escuela o psicólogos externos, y un grupo control. Resultados: los hallazgos sugieren que en los dos programas los profesores obtienen mejores resultados sobre el consumo de alcohol y la preocupación por la adicción. Conclusiones: se concluye que los profesores son los agentes aplicadores ideales en términos de eficiencia y discutimos las implicaciones que esto tiene para la práctica investigativa y práctica.

Determinación de parámetros hematoquímicos en pacientes VIH-SIDA tratados con antirretrovirales / Determination of hematochemical parameters in HIV/AIDS patients treated with antiretrovirals

Introducción: la terapia con antirretrovirales ha tenido un gran impacto en la calidad de vida de las personas que viven con VIH/SIDA. Los pacientes tratados con estos fármacos necesitan una vigilancia estricta debido a sus efectos tóxicos. Métodos: se realizó un estudio de 50 pacientes VIH/SIDA con tratamiento antirretroviral, atendidos en el hospital Dr. Juan Bruno Zayas entre mayo y septiembre del 2011. Se determinó la variación de parámetros hemato-bioquímicos que incluyeron estudios de perfil lipídico, hemático y renal. Resultados: las mayores afectaciones de los lípidos estuvieron en los pacientes del esquema Rescate. Todos los esquemas mostraron alteraciones de las proteínas totales, la microalbuminuria y la mayoría de los pacientes de los esquemas 4, 1 y Rescate presentaron anemia. Los pacientes estudiados presentaron alteraciones discretas de los parámetros analizados, Conclusiones: la toxicidad de los tratamientos aplicados está por debajo de la reportada en otras investigaciones(AU)

Introduction: Antiretroviral therapy has had a great impact on the quality of life of people living with HIV/AIDS, but these drugs require strict surveillance due to their toxic effects. Methods: A study was conducted of 50 HIV/AIDS patients under antiretroviral treatment cared for at Dr. Juan Bruno Zayas hospital from May to September 2011. Determination was performed of variations in hematochemical parameters, including lipid profiles and hematological and renal studies. Results: Most alterations in lipids were found in patients from the Rescue scheme. All schemes showed alterations in total proteins and microalbuminuria, whereas most patients in Schemes 4, 1 and Rescue had anemia. The patients studied presented moderate alterations of the parameters analyzed. Conclusions: Toxicity of the treatments applied is below that reported by other studies(AU)

Determinación de parámetros hematoquímicos en pacientes VIH-SIDA tratados con antirretrovirales / Determination of hematochemical parameters in HIV/AIDS patients treated with antiretrovirals

INTRODUCCIÓN: la terapia con antirretrovirales ha tenido un gran impacto en la calidad de vida de las personas que viven con VIH/SIDA. Los pacientes tratados con estos fármacos necesitan una vigilancia estricta debido a sus efectos tóxicos. MÉTODOS: se realizó un estudio de 50 pacientes VIH/SIDA con tratamiento antirretroviral, atendidos en el hospital Dr. Juan Bruno Zayas entre mayo y septiembre del 2011. Se determinó la variación de parámetros hemato-bioquímicos que incluyeron estudios de perfil lipídico, hemático y renal. RESULTADOS: las mayores afectaciones de los lípidos estuvieron en los pacientes del esquema Rescate. Todos los esquemas mostraron alteraciones de las proteínas totales, la microalbuminuria y la mayoría de los pacientes de los esquemas 4, 1 y Rescate presentaron anemia. Los pacientes estudiados presentaron alteraciones discretas de los parámetros analizados, CONCLUSIONES: la toxicidad de los tratamientos aplicados está por debajo de la reportada en otras investigaciones.

INTRODUCTION: Antiretroviral therapy has had a great impact on the quality of life of people living with HIV/AIDS, but these drugs require strict surveillance due to their toxic effects. METHODS: A study was conducted of 50 HIV/AIDS patients under antiretroviral treatment cared for at Dr. Juan Bruno Zayas hospital from May to September 2011. Determination was performed of variations in hematochemical parameters, including lipid profiles and hematological and renal studies. RESULTS: Most alterations in lipids were found in patients from the Rescue scheme. All schemes showed alterations in total proteins and microalbuminuria, whereas most patients in Schemes 4, 1 and Rescue had anemia. The patients studied presented moderate alterations of the parameters analyzed. CONCLUSIONS: Toxicity of the treatments applied is below that reported by other studies.

Autorregulação e consumo de substâncias na adolescência / Self-regulation and substance use in adolescence

O objetivo deste estudo é avaliar o papel da autorregulação desenvolvimental na adolescência como fator diferenciador do consumo de tabaco e álcool. Para isso, foi utilizada uma amostra de 407 alunos do Ensino Médio, avaliada com o recurso a um conjunto de questões sobre o consumo destas substâncias e ao Inventário de Autorregulação Adolescente (Moilanen, 2007), um instrumento baseado na teoria desenvolvimental sobre a autorregulação. Os resultados sugerem que a autorregulação é um fator diferenciador entre adolescentes que consomem, ou não, substâncias e a intensidade deste consumo. Adolescentes que consomem tendem a apresentar, consistentemente, pontuações mais baixas na autorregulação a curto e longo-prazo. As limitações e implicações dos resultados são apresentadas e discutidas.(AU)

The purpose of this study is to evaluate the role of developmental self-regulation in adolescence as a differentiating factor of tobacco and alcohol use. It was used a sample of 407 high school students, assessed with a number of questions about such substances use and the Adolescent Self-regulatory Inventory (Moilanen, 2007), a developmentally based measure about self-regulation. Results suggest that self-regulation is a differentiating factor between adolescents who use substances and those who not, and the intensity of consumption. Teenagers who use alcohol and tobacco seem to present consistently lower scores in short and long-term self-regulation. The limitations and implications of the results are presented and discussed.(AU)

Autorregulação e consumo de substâncias na adolescência / Self-regulation and substance use in adolescence

O objetivo deste estudo é avaliar o papel da autorregulação desenvolvimental na adolescência como fator diferenciador do consumo de tabaco e álcool. Para isso, foi utilizada uma amostra de 407 alunos do Ensino Médio, avaliada com o recurso a um conjunto de questões sobre o consumo destas substâncias e ao Inventário de Autorregulação Adolescente (Moilanen, 2007), um instrumento baseado na teoria desenvolvimental sobre a autorregulação. Os resultados sugerem que a autorregulação é um fator diferenciador entre adolescentes que consomem, ou não, substâncias e a intensidade deste consumo. Adolescentes que consomem tendem a apresentar, consistentemente, pontuações mais baixas na autorregulação a curto e longo-prazo. As limitações e implicações dos resultados são apresentadas e discutidas.

The purpose of this study is to evaluate the role of developmental self-regulation in adolescence as a differentiating factor of tobacco and alcohol use. It was used a sample of 407 high school students, assessed with a number of questions about such substances use and the Adolescent Self-regulatory Inventory (Moilanen, 2007), a developmentally based measure about self-regulation. Results suggest that self-regulation is a differentiating factor between adolescents who use substances and those who not, and the intensity of consumption. Teenagers who use alcohol and tobacco seem to present consistently lower scores in short and long-term self-regulation. The limitations and implications of the results are presented and discussed.

Temporal dynamics of Trichodesmium erythraeum (Cyanophyta) in the National Park "Sistema Arrecifal Veracruzano" in the Gulf of Mexico.

Trichodesmium erythraeum Ehrenberg ex Gomont is a microalga worldwide distributed in tropical seas. This filamentous microalga was detected in phytoplankton samples collected in the Protected NaturalArea "Parque Nacional SistemaArrecifal Veracruzano", and because of its ecological importance, its temporal dynamics was studied using net samples (30 microm) collected in one year period. Samples were studied with a light microscope recording the presence, morphological characteristics, life form and relative abundance. Trichodesmium erythraeum occurred as single filaments and was rare in April 2007 and March 2008; as single filaments from May to August and December 2007; as single filaments and small aggregates in September 2007 and January 2008; in aggregates of large size (> 3mm) and high relative abundance in October 2007; and was absence in November 2007 and April 2008. Although the relative abundance of the species was not important comparing with other members of phytoplankton, its occurrence was frequent with a bloom at the beginning of the north winds period. The observed temporal dynamics of this microalga in this coral reef region comes up on the alert in the monitoring red tides programs being implemented in Veracruz coast.

Simulating complex ion channel kinetics with IonChannelLab.

In silico simulation based on Markov chains is a powerful way to describe and predict the activity of many transport proteins including ion channels. However, modeling and simulation using realistic models of voltage- or ligand-gated ion channels exposed to a wide range of experimental conditions require building complex kinetic schemes and solving complicated differential equations. To circumvent these problems, we developed IonChannelLab a software tool that includes a user-friendly Graphical User Interface and a simulation library. This program supports channels with Ohmic or Goldman-Hodgkin-Katz behavior and can simulate the time-course of ionic and gating currents, single channel behavior and steady-state conditions. The program allows the simulation of experiments where voltage, ligand and ionic concentration are varied independently or simultaneously.

Hipertensión arterial, toxoplasmosis cerebral, microsporidiasis y SIDA / Hypertension, cerebral toxoplasmosis, microsporidiasis and aids

Se describe el caso de una paciente de 56 años de edad, que ingresó en el Servicio de Medicina Interna del Hospital Clinicoquirúrgico Juan Bruno Zayas por una emergencia hipertensiva (cefalea, vómitos, lenguaje disártrico, disminución de la fuerza muscular en hemicuerpo derecho y cifras de tensión arterial de 200/140 mm de Hg) y trastornos psicológicos; cuadro clínico que se inició días después de diagnosticarle el VIH/sida. A pesar de todos los esfuerzos realizados por mejorar su calidad de vida, fue remitida al Instituto de Medicina Tropical Pedro Kourí, donde falleció a causa de microsporidiosis renal, hepática, bronquial y vesical; enterocolitis inespecífica, gastritis aguda con ligera hemorragia difusa, hipertrofia concéntrica del ventrículo izquierdo por probable estenosis mitral y bronconeumonía focal(AU)

Hipertensión arterial, toxoplasmosis cerebral, microsporidiasis y SIDA / Hypertension, cerebral toxoplasmosis, microsporidiasis and aids

Se describe el caso de una paciente de 56 años de edad, que ingresó en el Servicio de Medicina Interna del Hospital Clinicoquirúrgico Juan Bruno Zayas por una emergencia hipertensiva (cefalea, vómitos, lenguaje disártrico, disminución de la fuerza muscular en hemicuerpo derecho y cifras de tensión arterial de 200/140 mm de Hg) y trastornos psicológicos; cuadro clínico que se inició días después de diagnosticarle el VIH/sida. A pesar de todos los esfuerzos realizados por mejorar su calidad de vida, fue remitida al Instituto de Medicina Tropical Pedro Kourí, donde falleció a causa de microsporidiosis renal, hepática, bronquial y vesical; enterocolitis inespecífica, gastritis aguda con ligera hemorragia difusa, hipertrofia concéntrica del ventrículo izquierdo por probable estenosis mitral y bronconeumonía focal