ABSTRACT
As part of a parasitological survey, several specimens of two new monopisthocotylean species, Neotetraonchus celsomanueli sp. nov. and N.peruvianus sp. nov. (Dactylogyridea, Dactylogyridae), were collected from the gill filaments of the Peruvian sea catfish Galeichthys peruvianus (Siluriformes, Ariidae) off Puerto Pizarro, Tumbes region, Peru. Neotetraonchus celsomanueli sp. nov. is characterised by an MCO with a T-shaped distal end and an accessory piece that is ribbed and expanded proximally with a worm-shaped termination. Neotetraonchus peruvianus sp. nov. is typified by its MCO, which has a sledgehammer-shaped distal end and an accessory piece with a claw-shaped distal end. Additionally, N.peruvianus sp. nov. is characterised by its jellyfish-shaped onchium. A partial 28S rDNA sequence was obtained from N.celsomanueli sp. nov., and a phylogenetic analysis was conducted. This analysis revealed the phylogenetic position of Neotetraonchus celsomanueli sp. nov. within a clade comprising monopisthocotylean parasites of diadromous and marine ariid catfishes, including Hamatopeduncularia spp., Chauhanellus spp., Thysanotohaptor Kritsky, Shameem, Kumari & Krishnaveni, , and Neocalceostomoides spinivaginalis Lim, 1995. This finding brings the number of known Neotetraonchus species to seven and represents the first described Neotetraonchus species infecting marine catfishes from Peru.
Subject(s)
Catfishes , Fish Diseases , Gills , Phylogeny , Animals , Catfishes/parasitology , Peru , Fish Diseases/parasitology , Gills/parasitology , Trematode Infections/veterinary , Trematode Infections/parasitology , DNA, Ribosomal/genetics , Trematoda/classification , Trematoda/genetics , Trematoda/anatomy & histology , Trematoda/isolation & purification , DNA, Helminth/genetics , RNA, Ribosomal, 28S/genetics , Platyhelminths/classification , Platyhelminths/genetics , Platyhelminths/anatomy & histology , Platyhelminths/isolation & purification , Sequence Analysis, DNAABSTRACT
Multicenter collaborative networks are essential for advancing research and improving clinical care for a variety of conditions. Research networks are particularly important for central nervous system infections, which remain difficult to study due to their sporadic occurrence and requirement for collection and testing of cerebrospinal fluid. Establishment of long-term research networks in resource-limited areas also facilitates diagnostic capacity building, surveillance for emerging pathogens, and provision of appropriate treatment where needed. We review our experience developing a research network for encephalitis among twelve hospitals in five Peruvian cities since 2009. We provide practical suggestions to aid other groups interested in advancing research on central nervous system infections in resource-limited areas.
ABSTRACT
Herpes simplex virus (HSV) is one of the most commonly identified infectious aetiologies of encephalitis in North America and Europe. The epidemiology of encephalitis beyond these regions, however, is poorly defined. During 2009-2012 we enrolled 313 patients in a multicentre prospective study of encephalitis in Peru, 45 (14·4%) of whom had confirmed HSV infection. Of 38 patients with known HSV type, 84% had HSV-1 and 16% had HSV-2. Patients with HSV infection were significantly more likely to present in the summer months (44·4% vs. 20·0%, P = 0·003) and have nausea (60·0% vs. 39·8%, P = 0·01) and rash (15·6% vs. 5·3%, P = 0·01) compared to patients without HSV infection. These findings highlight differences in the epidemiology and clinical presentation of HSV encephalitis outside of the Northern Hemisphere that warrant further investigation. Furthermore, there is an urgent need for improved HSV diagnostic capacity and availability of intravenous acyclovir in Peru.
Subject(s)
Encephalitis, Herpes Simplex/epidemiology , Simplexvirus/isolation & purification , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Encephalitis, Herpes Simplex/pathology , Encephalitis, Herpes Simplex/virology , Female , Humans , Infant , Male , Middle Aged , Peru/epidemiology , Prospective Studies , Seasons , Simplexvirus/classification , Young AdultABSTRACT
The purpose of the study was to determine prospectively the diagnostic value of a computed radiography (CR) system by comparing mammographic hard copy images with screen-film mammography (SFM). A series of 100 patients, who came for diagnostic investigation, underwent two-view SFM (Lorad M-IV Platinum) and digital mammography with a CR system (AGFA CR system). The images were obtained by double exposure, i.e. same view without removing compression of the corresponding breast. The CR images were processed with dedicated processing for mammography. Six radiologists read sets of SFM and CR images. The primary efficacy parameter was the overall diagnostic value. The secondary efficacy parameters were lesion conspicuity and lesion details (for masses and micro-calcifications), tissue visibility at chest wall and at skin line, axillary details, overall density and sharpness impression and the overall noise impression. These parameters were scored by a 7-point scoring system. "CR non-inferior to SFM" was concluded if the lower confidence interval bound exceeded 80%. The confidence interval for the overall diagnostic value was between 96.4% and 100%. Pooled analysis of the ten features for image quality comparison demonstrated for all but one feature (lesion details of the calcifications) CR non-inferiority to SFM.
Subject(s)
Mammography/methods , Tomography, X-Ray Computed/methods , X-Ray Intensifying Screens , Adult , Aged , Aged, 80 and over , Artifacts , Female , Humans , Image Processing, Computer-Assisted/methods , Male , Middle Aged , Observer Variation , Prospective Studies , Radiographic Image Enhancement/methodsABSTRACT
The genetic variability of a Quechua-speaking Andean population from Peru was examined on the basis of four Y chromosome markers and restriction sites that define the Amerindian mitochondrial DNA (mtDNA) haplogroups. Forty-nine out of 52 (90.4%) individuals had mtDNA which belonged to one of the four common Amerindian haplogroups, with 54% of the samples belonging to haplogroup B. Among 25 males, 12 had an Amerindian Y chromosome, which exists as four haplotypes defined on the basis of the DYS287, DYS199, DYS392 and DYS19 markers, three of which are shared by Amazonian Amerindians. Thus, there is a clear directionality of marriages, with an estimated genetic admixture with non-Amerindians that is 9 times lower for mtDNA than for Y chromosome DNA. The comparison of mtDNA of Andean Amerindians with that of people from other regions of South America in a total of 1,086 individuals demonstrates a geographical pattern, with a decreasing frequency of A and C haplotypes and increasing frequency of the D haplotype from the north of the Amazon River to the south of the Amazon River, reaching the lowest and the highest frequencies, respectively, in the more southern populations of Chile and Argentina. Conversely, the highest and lowest frequencies of the haplogroup B are found, respectively, in the Andean and the North Amazon regions, and it is absent from some southern populations, suggesting that haplotypes A, C and D, and haplotype B may have been dispersed by two different migratory routes within the continent.
Subject(s)
DNA, Mitochondrial/genetics , Emigration and Immigration , Genetic Variation , Indians, South American/genetics , Y Chromosome , Female , Haplotypes , Humans , Male , Peru/ethnology , Polymorphism, Restriction Fragment LengthABSTRACT
Neovascularizaçäo de córnea (NC) é considerado um dos maiores fatores de risco para rejeiçäo em transplantes de córnea. Várias formas de tratamento para NC tem sido sugeridas incluindo esteróides, irradiaçäo, tiotepa, ciclorioterapia e outros. Tratamento para NC usando Argon e YAG laser, tem melhorado o prognóstico de transplante de córnea e parece ser seguro e eficaz. Näo há, porém, na literatura pesquisada, estudo comparando as duas formas de tratamento. Em estudo randomizado, comparamos as duas formas de tratamento para NC usando Argon e YAG laser para avaliar sua segurança e eficácia, em pacientes com NC refratária ao tratamento convencional. Em nosso estudo, Argon laser mostrou ser terapia alternativa e/ou complentar útil no tratamento ..