ABSTRACT
Atualmente novos princípios ativos de função reabsortiva têm ganhado campo de estudo para avaliar seus mecanismos e comportamento biológico. Com isso, um novo antirreabsortivo, inibidor da catepsina K tem apresentado efeito positivo na osseointegração. Este estudo tem como objetivo avaliar a resposta óssea da superfície de implantes revestida por duplo ataque ácido e Odanacatib (MK-0822) em ratas ovariectomizadas. Neste estudo foram utilizadas ratas (Albinus, Wistar) ovariectomizadas ou sham (placebo). Cinquenta e dois (52) tiveram as superfícies revestidas por duplo ataque ácido e MK-0822 a 0,06 mg/ml através do método biomimético, e 48 implantes foram instalados em tíbias de ratas ovariectomizadas ou sham. Microscopia eletrônica de varredura (MEV) e energia dispersiva de raios-x (EDS) foram realizadas em 4 implantes após tratamento de superfície, para análise da topografia e composição química, além da realização da análise do ângulo de contato em 16 discos de titânio comercialmente puro tratados com as mesmas superfícies. Aos 15 e 40 dias após instalação de implantes (n=6), foi realizada microtomografia computadorizada. Dados quantitativos foram avaliados adotando-se o nível de significância p< 0,05. Além dos resultados topográficos favoráveis para os grupos tratados com MK-0822, os resultados microtomográficos apresentaram diferença estatisticamente significante entre os grupos SHAM e OVX na maioria dos parâmetros (p< 0,05). Ainda assim, os grupos tratados com MK-0822 apresentaram resultado semelhante ou maior, porém sem diferença estatística, em relação ao grupo controle em todos os parâmetros (TV, BV, BV ̸TV, Tb.Sp e Tb.N)(AU)
Currently new active principles of resorptive function have gained field of study to evaluate their mechanisms and biological behavior. Thereby a new anti-absorbent, cathepsin K inhibitor has had a positive effect on osseointegration. This study aims to evaluate the bone response of the surface of implants coated by double acid-etched and Odanacatib (MK-0822) in ovariectomized rats. In this study, either ovariectomized rats (Albinus, Wistar) or sham (placebo) have been used. Fifty-two (52) implants had the surfaces coated with double acid-etched and MK-0822 at 0,06 mg/ml by the biomimetic method and 48 implants were installed on sham or ovariectomized rat tibias. Scanning electron microscopy (SEM) and X-ray dispersive energy (EDS) were performed in 4 implants after surface treatment for analysis of topography and chemical composition, in addition to performing contact angle analysis on 16 commercially pure titanium discs treated with the same surfaces. At 15 and 40 days after implant installation, microcomputer tomography was performed. Quantitative data was evaluated by adopting the significance level of p< 0.05. Besides the favorable topographic results to MK-0822 coated implants group, the microtomographic results presents statistically significant differences between the SHAM and OVX groups at most of the parameters (p< 0,05). Nevertheless, the MK-0822 coated group presents similar or higher values, although without statistic differences related to the control group in all parameters (TV, BV, BV ̸TV, Tb.Sp and Tb.N)(AU)
Subject(s)
Animals , Rats , Surface Properties , Bone Resorption , Dental Implants , Rats, Wistar , Cathepsin KABSTRACT
(1) Background: The tissue engineering field has been working to find biomaterials that mimic the biological properties of autogenous bone grafts. (2) Aim: To evaluate the osteoconduction potential of injectable calcium phosphate cement implanted in critical defects in rat calvaria. (3) Methods: In the calvarial bone of 36 rats, 7-mm diameter critical size defects were performed. Afterwards, the animals were randomly divided into three groups according to filler material: a blood clot group (BC), blood clot membrane group (BCM), and an injectable ß-tricalcium phosphate group (HBS) cement group. After periods of 30 and 60 days, the animals were euthanized, the calvaria was isolated, and submitted to a decalcification process for later blades confection. Qualitative and quantitative analysis of the neoformed bone tissue were conducted, and histometric data were statistically analyzed. (4) Results: Sixty days post-surgery, the percentages of neoformed bone were 10.67 ± 5.57 in group BC, 16.71 ± 5.0 in group BCM, and 55.11 ± 13.20 in group HBS. The bone formation values in group HBS were significantly higher (p < 0.05) than in groups BC and BCM. (5) Conclusions: Based on these results, it can be concluded that injectable calcium phosphate cement is an osteoconductive material that can be used to fill bone cavities.
ABSTRACT
A proposta deste estudo foi avaliar o processo de regeneração óssea com o uso do biomaterial Biogran®, um vidro bioativo, como uma opção de substituto ósseo para técnicas de enxerto em levantamento do assoalho do seio maxilar através de análises histológica e histomorfométrica. Para tal, 24 coelhos da raça Nova Zelândia foram submetidos ao procedimento de levantamento do seio maxilar bilateralmente, sendo divididos em dois grupos: grupo coágulo implante (GCI), no qual foi realizado o levantamento do seio maxilar, seguindo implante imediato, sem enxerto; e o grupo biovidro implante (GBI), no qual foi realizado o levantamento do seio maxilar, preenchimento com Biogran® e instalação de implante imediata. Os animais foram submetidos à eutanásia aos 7, 15 e 40 dias. A análise histológica mostrou no grupo GCI uma formação de tecido ósseo gradual, sendo maior aos 40 dias, com característica lamelar. Porém, não apresentava diferença estatística entre os períodos de 15 e 40 dias de eutanásia (p=0,210). O grupo GBI revelou uma formação de tecido ósseo mais tardia, com maior quantidade aos 40 dias, com reabsorção lenta e progressiva dos grânulos do biomaterial e sinais indicativos de atividade osteoblástica. Este grupo mostrou diferença estatística entre os períodos de 7 e 15 dias quando comparados aos 40 dias (p<0,05). Na análise intergrupos, não houve diferença estatisticamente significante aos 7 e aos 40 dias, mas sim, aos 15 dias. Dessa forma, pode-se concluir que o Biogran® é um biomaterial com propriedades osteocondutoras, de reabsorção lenta, capaz de produzir tecido ósseo satisfatório. Porém, com quantidade semelhante ao coágulo aos 40 dias, o que gera maiores discussões e a necessidade de mais estudos para comprovar a eficácia desse biomaterial a longo prazo(AU)
The purpose of this study was to evaluate the bone regeneration process with the use of the Biogran® biomaterial, a bioactive glass, as a bone substitute option for grafting techniques in the maxillary sinus floor through histological and histomorphometric analysis. To that end, 24 New Zealand rabbits underwent a bilateral maxillary sinus removal procedure, and were divided into two groups: an implant clot group (ICG), in which the maxillary sinus was removed, followed by immediate implantation without graft ; and the implant bioglass group (GBI), in which the maxillary sinus was removed, filled with Biogran® and implanted in the immediate implant. The animals were submitted to euthanasia at 7, 15 and 40 days. The histological analysis showed a formation of gradual bone tissue in the GCI group, being larger at 40 days, with a lamellar characteristic. However, there was no statistical difference between the periods of 15 and 40 days of euthanasia (p = 0.210). The GBI group revealed a later bone formation with a greater amount at 40 days, with slow and progressive resorption of the biomaterial granules and signs indicative of osteoblastic activity. This group showed statistical difference between the periods of 7 and 15 days when compared to the 40 days (p <0.05). In the intergroup analysis, there was no statistically significant difference at 7 and 40 days, but at 15 days. Thus, it can be concluded that Biogran® is a biomaterial with osteoconductive properties, of slow resorption, able to produce satisfactory bone tissue. However, with a similar amount to the clot at 40 days, which generates more discussions and the need for further studies to prove the efficacy of this biomaterial in the long term(AU)
Subject(s)
Animals , Rabbits , Bone Regeneration , Bone Substitutes , Maxillary Sinus , Biocompatible MaterialsABSTRACT
Frontal bone fractures represent a low percentage of craniofacial fractures. However, a systematic approach and a correct diagnosis are essential for successful treatment and maintenance of physiology of the frontal sinus and late complications. The purpose of this study was to report a clinical patient with anterior wall fracture of the frontal due to sports accident sinus that was surgically treated.