ABSTRACT
BACKGROUND: Secondary salinized soil in greenhouses often contains excess nitrate. Inoculation of Bacillus megaterium NCT-2 with nitrate assimilation ability represents an attractive approach for soil remediation. However, the effects of NCT-2 on the structure and function of soil microbial communities have not been explored. METHODS: Greenhouse experiments were carried out to investigate changes in soil properties, Brassica chinensis L. growth, bacterial, and fungal community structure and function in response to NCT-2 inoculation. RESULTS: The NCT-2 inoculant significantly reduced the nitrate content in B. chinensis and inhibited the rebound of soil nitrate in the later stage. The shifts of bacterial community structure and function by NCT-2 was negligible, and a greater disturbance of soil fungal community structure and function was observed, for example the strong inhibitory effect on ectomycorrhizal fungi. These results indicated that the NCT-2 inoculant likely achieved the remediation effect in secondary salinized soil by shifting fungal community. The present findings add to the current understanding of microbial interactions in response to bacterial inoculation and can be of great significance for the application of NCT-2 inoculants in secondary salinized soil remediation.
ABSTRACT
The salinity characteristics of greenhouse soils with cropping obstacles in Shanghai suburb were investigated and analyzed. The salinity contents of the salinization greenhouse soils showed a trend of first increasing and then decreasing with the increasing cropping duration. The salinized soils mainly included slightly salted, mildly salted and salted soils, which accounted for 17.39%, 56.52% and 13.04%, respectively. Among them, the degree of salinity in greenhouse soil planted with asparagus in Chongming County was the highest. Among the salt ions in greenhouse soils, the cations were mainly Ca2+ and Na+, while the anions were mainly NO3- and SO4(2-). The degree of salinity was mainly influenced by fertilization mode, cropping duration, crop type and management level, which led to the great variation in the salinity contents and salt ions. Canonical correspondence analysis found that the contents of Ca2+, Mg2+ and NO3- in greenhouse soils were greatly affected by cropping duration, and the degree of salinity would be enhanced and attenuated with long-term application of single fertilizer and mixed application of chemical fertilizer and organic manure, respectively. The greenhouse soils in Shanghai suburb could be classified as four patterns influenced by the relationship between salinity ions and samples, and the most soils were influenced by Ca2+, Mg2+, NO3- and Cl-, which required to be primarily controlled.
