Development and evaluation of onion bulb descaler for removal and collection of surface dry peel.

Onion bulbs contain outer papery dry peel which is rich with industrially important high-value compounds. Removal of dry peel/scale of onion is the common practice during bulb processing, storage and marketing. A conventional manual method of scale removal involves huge labour, drudgery and means of environmental pollution. An onion descaler machine has been therefore developed to mechanize this operation, reduce drudgery and effectively collect the dry scales for industrial use. The operating parameters of the developed machine were optimized for its best performance using RSM. During optimization study, roller speed, roller slope and hopper opening were taken as independent variables. The performance of the machine was evaluated through descaling efficiency and descaling loss. During experimentation, descaling efficiency and descaling loss were obtained in the range of 35.92-98.55% and 0.82-9.07%. The optimum performance of the machine was obtained at 450 rpm roller speed, 2% roller slope and 380 mm of the hopper opening with maximum descaling efficiency (88.29%) and minimum descaling loss (3.65%). The optimization of the onion descaler parameters and their statistical relation provided the necessary information on the combination of material-machine alignment, and design parameters for enhanced descaling efficiency. Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-021-05264-5.

Effect of environment on spatio-temporal structuring of fish assemblages in a monsoon-influenced tropical estuary.

This study analyzes the trophic structure of fish assemblages in a well-mixed tropical estuary, Zuari, located in southwest coast of India. A total of 224 fish species were collected between September 2013 and August 2016 from 324 sampling events from 9 sampling stations using surface and bottom set gillnets. The sampling events were designed to cover three major seasons such as the pre-monsoon, monsoon, and post-monsoon. For each sampling event, abiotic variables such as temperature, salinity, dissolved oxygen, and transparency of the water were recorded. The data obtained for abundance of fish species was subjected to cluster analysis and non-metric multidimensional scaling. The results defined six spatio-temporally varying groups (clusters) within Zuari estuary. Canonical correspondence analysis demonstrated that temperature, salinity, dissolved oxygen, and transparency were important in determining the fish assemblages in Zuari. The estuarine resident species were abundant in the inner zone (riverine side) of the estuary, where the salinity and temperature recorded lower dimensions compared to middle and outer zones (marine side). However, the inner shallow zone of the estuary was not a preferred habitat for the migrating marine species. Majority of the fish species preferred middle and outer zones and post-monsoon and pre-monsoon seasons for inhabiting the estuary. The present study furnishes a reference data for the spatio-temporal dynamics of fish assemblages in tropical monsoonal estuaries.

Baseline radionuclide concentration in selected marine organisms around the coastal areas of Ratnagiri and Sindhudurg districts, west coast of Maharashtra, India.

The present work deals with assessment of baseline radionuclide concentration in marine organisms around selected coasts of Maharashtra, India. This baseline study highlights concentrations of natural and fallout radionuclides in finfish and shellfish species found in the surrounding seawater. Water and fish samples were collected, processed, and analyzed for 238U, 226Ra, 228Ra, 232Th, 40K, and 137Cs by high-resolution gamma spectrometry. 238U, 226Ra, 228Ra, 232Th, 40K, and 137Cs concentrations were found to be in the range of ≤1.5, ≤2.0, ≤0.4, ≤1.5, ≤8.0 to 258, and ≤0.2 Bq/kg, respectively. The seasonal study revealed the maximum concentration in premonsoon season. Activity concentrations were higher in pelagic fish than those in demersal fish. A higher concentration factor was reported in shellfish than in finfish.

Maturation profile and fecundity of the exotic Oreochromis niloticus in the River Yamuna, India.

The knowledge on the reproductive biology of fish Oreochromis niloticus (Linnaeus, 1758) in riverine condition is meagre in the Indian sub-continent which is necessary for the development of an appropriate fishery management protocols for the rivers. In this context, the gonadal maturation profile and fecundity of 0. niloticus was studied. Mature, spawning and spent females recorded in almost all the months of the year evidenced multiple and prolonged breeding behaviour. An overall sex ratio (M/F) of 1: 1.08 observed was statistically non-significant from expected 1:1 ratio (chi2=6.994219, p=0.8642) indicating that males and females were statistically equal in number. The absolute fecundity ranged from 1192 to 4760 with mean of 2590 eggs from ovary weighing between 1.91 g to 28.89 g. Monthly changes in gonado-somatic index (GSI) revealed that fish bred throughout the year except in July and August, but at peak between October-November and March-June. Size at first maturity of male was estimated as 229.6 mm with confidence limit of 235.9 and 223.6 mm and for female as 238.1 mm with confidence limit of 244.7 and 231.7 mm, respectively.

DNA barcoding of gobiid fishes (Perciformes, Gobioidei).

Gobiids constitute a major proportion of fish population in both tropical and temperate freshwater as well as marine ecosystem. Due to their small size, cryptic ecology and ambiguous morphological characters, gobiids diversity was not documented completely. In this study, DNA barcodes were generated for 11 species of gobiids, collected from the Ashtamudi Lake, India. The mitochondrial COI gene was amplified using universal primers and the resulted 650 bp amplicon was sequenced. The COI barcodes clearly distinguished all the species with high inter-specific genetic distance values than intra-specific values based on K2P (Kimura 2 Parameter) model. The average genetic distance (K2P model) within species, genus and family was 1.2%, 22.2% and 25.3%, respectively. In addition to barcode-based species identification system, Nucleotide Diagnostic (ND) characters specific for species were identified. The Neighbor-Joining tree revealed distinct clusters shared by the species of same genera.

DNA barcoding of elasmobranchs from Indian coast and its reliability in delineating geographically widespread specimens.

Identification of elasmobranchs by conventional taxonomy is difficult due to similarities in morphological characters. Species-specific molecular markers are good choice for identifying species irrespective of it's life stage. Recently, mitochondrial cytochrome c oxidase subunit I (COI) gene got global recognition as a barcode gene to discriminate all animals up-to species level. In this study, mitochondrial COI partial gene was used to develop DNA barcodes for 18 species of elasmobranchs (10 species of sharks and 8 species of rays). The COI barcodes clearly distinguished all the species with high interspecific distance values than intraspecific values. The average interspecific and intraspecific distance values are 8.6% and 0.3% for sharks, respectively and 12.4% and 0.63% for rays, respectively using K2P method. The Neighbor-Joining tree showed distinct clusters shared by the species of same genera. The COI barcodes were also used to estimate allopatric divergences for selected species across broad geographical locations and found that Sphyrna lewini, Aetobatus narinari and Neotrygon kuhlii have cryptic diversity.

Cord blood stem cells - a dream for future medicine.

Umbilical cord blood collected at birth is a rich source of stem cells that can be used to treat diseases of the blood and immune system. Cord blood stem cells which have infinite medical potential are currently used in the treatment of several life-threatening diseases, and play an important role in the treatment of blood and immune system related genetic diseases, cancers, and blood disorders. Transplant recipients are less likely to reject a cord blood stem cell and do not require a perfect match as bone marrow transplants do. Transplantation of umbilical cord blood stem cells represents a major advantage in providing a new source of stem cells to patients in need. Private storage of cord blood stem cells clearly has value, in that it provides future patients and families with potential therapeutic options for transplant. Many recent studies showed that human umbilical cord blood stem cells have the potential to generate cells with neuronal characteristics. Therefore, the umbilical cord blood stem cells can be viewed as the stem cells source of choice for clinical and non-clinical research applications.

Ultrastructural alterations in the gills of Labeo rohita fingerlings exposed to thermal extremes.

This study aimed to determine the cellular alterations in the gill of Labeo rohita exposed to lethal temperature maxima (LTM ax ) and lethal temperature minima (LTM in ) by means of transmission electron microscopy (TEM). Acclimation of advanced fingerlings of L. rohita was carried out at 26°C for 30 days. Acclimated fish were subjected to a constant rate of increase or decrease in temperature (0.3°C/min) until the LTM ax and LTM in values were reached. Dissected gills were processed for TEM, both at the end of acclimation period at ambient temperature (26°C) and at lethal temperatures. Results indicated that at ambient temperature, the gill tissues appeared normal. However, significant changes were observed at lethal temperatures. The gill tissues at lethal temperature maxima showed severely damaged lamellae, with more vacuolated space. At lethal temperature minima, gill tissues showed increased density of mitochondria. Our prima-facie report indicated that L. rohita exposed to lethal temperatures exhibited marked ultrastructural changes in the gills.

Molecular phylogeny of elasmobranchs inferred from mitochondrial and nuclear markers.

The elasmobranchs (sharks, rays and skates) being the extant survivors of one of the earliest offshoots of the vertebrate evolutionary tree are good model organisms to study the primitive vertebrate conditions. They play a significant role in maintaining the ecological balance and have high economic value. Due to over-exploitation and illegal fishing worldwide, the elasmobranch stocks are being decimated at an alarming rate. Appropriate management measures are necessary for restoring depleted elasmobranch stocks. One approach for restoring stocks is implementation of conservation measures and these measures can be formulated effectively by knowing the evolutionary relationship among the elasmobranchs. In this study, a total of 30 species were chosen for molecular phylogeny studies using mitochondrial cytochrome c oxidase subunit I, 12S ribosomal RNA gene and nuclear Internal Transcribed Spacer 2. Among different genes, the combined dataset of COI and 12S rRNA resulted in a well resolved tree topology with significant bootstrap/posterior probabilities values. The results supported the reciprocal monophyly of sharks and batoids. Within Galeomorphii, Heterodontiformes (bullhead sharks) formed as a sister group to Lamniformes (mackerel sharks): Orectolobiformes (carpet sharks) and to Carcharhiniformes (ground sharks). Within batoids, the Myliobatiformes formed a monophyly group while Pristiformes (sawfishes) and Rhinobatiformes (guitar fishes) formed a sister group to all other batoids.

Microscopic impacts of gestational diabetes mellitus on the umbilical cord.

This observational study was done among the different types of diabetes, gestational diabetes mellitus (GDM). In the present study we looked for the microscopic impacts of GDM on the umbilical cord. This study was carried out in the department of Anatomy, Chittagong Medical College from May 2006 to April 2007. Total fifty umbilical cords with placenta were collected from selected subjects within 36 to 40th weeks of gestation and divided them into two groups. Twenty five control group (specimen collected from normal mothers) and 25 GDM group (specimen collected from mothers developing diabetes during pregnancy state). Tissues from a particular area of the umbilical cord were processed for histological study by routine procedure. All the slides were examined under light microscope. In GDM group light microscopy showed rupture and erosion of the endothelium and complete rupture of the umbilical vessels resulting in extravasation of blood within Wharton's jelly. The umbilical vein was grossly dilated and thin walled. Smooth muscles of the umbilical vessel showed disruption and degeneration of its fibers. Wharton's jelly showed alteration in the pattern of distribution and degeneration of its fibers with honeycomb like empty spaces among them. It is suggested that GDM might have some harmful effects on the umbilical vessels and connective tissue component of the Wharton's jelly.

Intertidal biodiversity and health status of Bandstand shore (Bandra), Mumbai, India.

A study was carried out to assess the biodiversity status of the intertidal region of rocky shore of Bandstand (Bandra), Mumbai. Among 42 species recorded, Euchelus asper was maximum in density during December (123/m2). However, maximum biomass was recorded for Nerita oryzarum in November (146.94 g/m2). ANOVA revealed no variation in the occurrence of organisms according to transects, but the variation was recorded according to months and quadrates. Shannon and Simpson's diversity index, Margalef's richness index and Pielou's evenness index indicated different level of ecological state of the shore in different months. Dendrogram from Bray-Curtis similarity matrix and non metric Multi-Diamentional Scaling (MDS) revealed maximum closeness of occurrence between N. oryzarum and Planaxis sulcatus. Shepard diagram and abundance/biomass comparison (ABC) curve method revealed light to moderately polluted status of the shore. However, in spite of such condition, this shore is still rich in intertidal biodiversity that should be conserved.

A comparative study on intertidal faunal biodiversity of selected beaches of Mumbai coast.

Comparative study has been done to examine the biodiversity and ecological status of the intertidal region of Tata Institute of Fundamental Research (TIFR), Bandstand and National Centre for Performing Arts (NCPA) rocky beaches in Mumbai, West coast of India. A total of 50 species of intertidal organisms were recorded from these shores. Shannon and Simpson's diversity index, Margalefs richness index and Pielou's evenness index indicated different level of ecological state of the shore in different months. Dendrograms and 2-D non metric MDS ordination from Bray-Curtis similarity matrix of occurrence of intertidal organisms from these sites showed highest similarity and combination pattern of occurrence between Nerita oryzarum and Planaxis sulcatus in TIFR and Bandstand shore. Nerita oryzarum and Tactarius malaccanus at NCPA shore. Abundance/biomass comparison (ABC) method of determining level of disturbance also pointed towards the polluted status of these shores. Study concludes that though these beaches are highly disturbed due to anthropogenic activities, they still support a rich intertidal biodiversity which need immediate attention for protection and conservation.

Biochemical and stress responses of rohu Labeo rohita and mrigal Cirrhinus mrigala in relation to acclimation temperatures.

The biochemical and stress responses of two Indian major carps, rohu Labeo rohita and mrigal Cirrhinus mrigala were studied after acclimating them to four preset temperatures (26, 31, 33 and 36 degrees C) for 30 days. The blood glucose and liver glycogen levels showed an inverse trend in both the species and were significantly different in L. rohita at higher temperatures. The decrease in the liver glycogen level of C. mrigala, however, was not significant. Plasma cortisol levels increased significantly whereas the ascorbic acid content in the brain and kidney of both the species decreased significantly with increasing temperatures. Total lipid content in the liver of both the species decreased significantly with increasing acclimation temperatures. The phospholipid concentration decreased in L. rohita with increasing acclimation temperatures, and in C. mrigala the values decreased up to 33 degrees C and increased at 36 degrees C. In C. mrigala, the cholesterol level decreased up to 33 degrees C and then increased at 36 degrees C, but the absolute value was lower in comparison to L. rohita. The cholesterol levels, however, were not significantly different in L. rohita. Triglycerides and free fatty acids concentrations decreased significantly with increasing acclimation temperatures in both the species. The present study indicates species-specific metabolic responses of L. rohita and C. mrigala to thermal acclimation.

Attitudinal response towards road traffic noise in the industrial town of Asansol, India.

The major objective of the investigation was to evaluate the road traffic noise and its likely impacts on the local community of Asansol city (West Bengal, India) by monitoring and modeling. The attitudinal response of local population due to existing vehicular noise is presented in the paper. Noise and Attitudinal Survey was conducted at 25 locations. A total of 869 individuals were surveyed. The relationship between traffic noise levels and annoyance was studied using correlation, linear and multiple linear regressions analysis. The average L(dn) value was 73.28 +/- 8.51 dB(A) (55.1-87.3); The Traffic Noise Index (TNI) was 80.62 +/- 15.88 dB(A) (49.4-115.8). The mean value of percent of population Highly Annoyed (%HA) due to road traffic noise was 26.50 +/- 3.37 (19.44-33.2), whereas the mean dissatisfaction score (MDS) was 2.96 +/- 0.90 (1.04-4.45). Annoyance modeling was also performed based on field data. It can be said that Noise values gives desirable annoyance predicting values in comparison to vehicular data.

Evaluation and analysis of road traffic noise in Asansol: an industrial town of eastern India.

The objectives of the study were to monitor and assess the road traffic noise in its spatial-temporal aspect in an urban area. The paper discusses the observations, results and their interpretation based on the study. Noise recordings from site, collected from April 2006 to March 2006, were used for statistical analysis and generation of various noise indices. Noise maps were also created for impact analysis and formulation of Noise Risk Zones. Mean Ldn value ranged between 55.1 and 87.3 dB (A). Day time Leq level ranged between 51.2 and 89.0 dB (A), where it ranged between 43.5 and 81.9 dB (A) during night. The study reveals that present noise level in all the locations exceeds the limit prescribed by CPCB. Based on the finding it can be said that the population in this industrial town are exposed to significantly high noise level, which is caused mostly due to road traffic.

Assessment of coastal pollution through bioassay and transplantation of intertidal clams.

In the present study, a survey of Mahim creek and Bay area was undertaken that indicated absence of fauna, particularly molluscs from the area, which was a repository in the past. During bioassay experiments of Mahim creek water, the clams G. divaricatum and C. antiquata could not open their valves in 100% creek water and died within 12 hrs of exposure. The 96 hrs LC50 values of Mahim creek water for G. divaricatum and C. antiquata were found to be 20% and 40% respectively during summer and 38% and 57% respectively during rainy season. When two sets of the clams were transplanted at Mahim creek, they died within 12 hrs. These experiments suggest the extreme level of pollution in the area. This level of pollution is responsible for transforming the area into barren locality in terms of fauna, specially the rich molluscan diversity. However, Gorai creek was found to be comparatively very less polluted and it still serves as breeding and nursery ground for various fishes and prawn species. Hence it must be protected and conserved.

Monthly variation in night time noise levels at residential areas of Asansol city (India).

Intense vehicular movement and industrial activities are the major noise producing sources in the Asansol city. Ambient night time noise levels were monitored in ten residential areas from August 2002 to July 2003, to study the existing quality of noise and their impact in the local community. Based on the monitored noise levels in the area, traffic-social survey was also conducted. About 39% of individuals surveyed were highly annoyed with noise pollution from different origins. The study revealed that night time noise levels (10.00 pm - 6.00 am) in all the locations exceeded the limit prescribed by Central Pollution Control Board. The day time noise level was much higher at all locations in respect of the night time noise level. The Day-Night equivalent noise level (Ldn) was determined and ranged between 67.16 dB(A) and 89.44 dB(A). The percentile noise level (L90) exceeded the CPCB standard in all cases and varied from 72.36 dB(A) to 102.45 dB(A). Based on the results of the study, it can be concluded that the local community is exposed to high noise environment.