Effects of nickel supplementation on nutrient utilization, mineral balance, haematology and antioxidant status of crossbred dairy calves.

BACKGROUND: Nickel has been identified as an important newer trace mineral playing essential role in animals however, its precise mechanism of action in the body is yet to be determined. Interaction of Ni with other essential minerals is suggested in reports limited to lab animals and needs to be explored further in large animals. AIM: This study was conducted to study the influence of Ni supplementation at different levels, on minerals and health status of crossbred dairy calves. METHOD: Twenty-four Karan Fries crossbred (Tharparkar × Holstein Friesian) male dairy calves were selected based on body weight (137.09 ± 5.68) and age (10.78 ± 0.61) and were divided into 4 treatment groups (n = 6) given basal diet supplemented with 0 (Ni0), 5 (Ni5), 7.5 (Ni7.5) and 10 (Ni10) ppm Ni/kg of DM. Nickel was supplemented in the form of nickel sulphate hexahydrate (NiSO4.6 H2O) solution. To ensure the intake of the required amount of nickel by each animal, the calculated quantity of solution was mixed with 250 g concentrate mixture and offered individually to the calves. The calves were fed total mixed ration (TMR) consisting of green fodder, wheat straw and concentrate mixture in the ratio of 40:20:40 and the nutritional requirements were met according to NRC (2001) guidelines. Growth performance was recorded at fortnightly interval whereas, plasma minerals, haematology, antioxidant and immunity parameters were studied at monthly interval during the 150-day experimental period. Nutrient utilization and mineral balances were estimated with the help of a metabolism trial conducted at the end of feeding trial. RESULTS: Supplementation of Ni exhibited no influence on dry matter intake (DMI), body weight, average daily gain (ADG) and nutrient digestibility of dairy calves. However, the absorption and balance of minerals such as Ni, Fe, Cu, Zn and their respective plasma concentration increased (P < 0.05) with Ni supplementation and highest values were observed in calves fed 10 mg Ni/kg DM. The red blood cell (RBC) count, haemoglobin (Hb) concentration, haematocrit (HCT) and activity of superoxide dismutase (SOD) and catalase antioxidant enzymes showed highest increase (P < 0.05) in calves supplemented with Ni at level of 10 mg/kg DM as compared to other treatment groups. However, white blood cell (WBC) count, glutathione peroxidase (GPx), total antioxidant status (TAS), total immunoglobulins and IgG plasma concentration remained unaltered with addition of different levels of Ni in the diet of calves. CONCLUSIONS: The supplementation of Ni at level of 10 mg/kg DM shows a positive effect on status of trace minerals such as Fe, Cu, Zn and improves the physiological conditions and health status of crossbred dairy calves indicated by improved haematology and antioxidant parameters.

Potential use of Tinospora cordifolia as a herbal supplement in dairy animals: a review.

Supplementation is an integral part of modern dairy-feeding practices which mainly emphasise on higher production. Different supplements have been utilized by the farmers according to the need and action of supplements. Tinospora cordifolia (TC) is one such herbal supplement which can be utilized to feed dairy animals either alone or in combination with other supplements. TC is a herb found in tropical countries and having number of medicinal properties. Beneficial health effects of TC include immunostimulation, protection against inflammation and bacterial action, hepatoprotection, antioxidant and antineoplastic effects. Available studies on TC supplementation in dairy animals have shown a positive effect on health, body parameters and production performance. Supplementing TC is economically feasible, and it can be adopted by farmers as it helps in improving their net income. However, more research is needed in the field of dairy animals to explore the full potential of this herb. This review is written with the objective of highlighting the possibilities of using TC in dairy animal rations and promoting research in the concerned field to fill the gaps in knowledge. Thorough knowledge about TC and its effect in dairy animals can add a new concept to the supplementation process and developments of dairy rations.

Effect of bypass fatty acid and Tinospora cordifolia supplementation on production performance and milk fatty acid profiling in Murrah buffaloes (Bubalus bubalis).

Buffaloes contribute about 49% of total milk production and are considered as major milk-producing animals in India. During the early stage of lactation, mostly high producing animals suffer from negative energy balance (NEB) due to imbalance between the needs and availability of energy, and this NEB has a negative impact on production and health of buffaloes. Taking this into consideration, a study was conducted at National Dairy Research Institute, Karnal on 20 freshly calved healthy Murrah buffaloes (Bubalus bubalis). Buffaloes were grouped depending on their previous milk yield, body weight, and parity as T0 (control), T1 (fatty acids), T2 (Tinospora), and T3 (mix) groups having five animals each. Supplementation of 150 g of bypass fatty acids in T1 group, 150 g of Tinospora powder in T2 group, and combination of 150 of bypass fatty acid and 150 g of Tinospora powder in T3 group over and above the standard feeding schedule was done for a duration of 90 days whereas standard feeding schedule was followed in T0 group. A significant (P < 0.05) increase in average daily milk yield was observed in T1 (10.06 ± 0.56 kg/d/h), T2 (10.30 ± 0.53 kg/d/h), and T3 (10.11 ± 0.48 kg/d/h) in comparison to control group (8.05 ± 0.38 kg/d/h). Significantly (P < 0.05) higher milk fat percent and total solids were also recorded in treatment groups over the control group. Other milk constituents (SNF, protein, lactose, ash) and milk properties (pH, conductivity, freezing point, density) remained unaffected by the supplementations. The somatic cell count values were significantly (P < 0.05) decreased in T2 and T3 with the advancement in the lactation in comparison to T0 and T1. Supplementation also leads to significantly (P < 0.05) higher MUFA and PUFA contents in milk of treatment group buffaloes in comparison to control group. The study outcomes state that combination of bypass fatty acids and Tinospora cordifolia supplementation improves milk production and its composition in lactating Murrah buffaloes.

Effect of feeding Azolla pinnata in combination with direct-fed microbial on broiler performance.

A study was conducted on broiler birds for 42 days to determine the effect of feeding azolla along with direct-fed microbial (DFM) on growth performance, nutrient utilization, biochemical parameters and carcass characteristics. A total of 360-day-old male broiler chicks were divided into 3 groups and 12 replicates with 10 chicks per replicate, according to complete randomized block design. One group was fed a diet containing 2.5% azolla (A) and another was fed a diet containing 2.5% azolla, with direct-fed microbial (A + DFM), viz. L. plantarum and L. rhamnosus, at the concentration of 13.4 × 108 and 1.52 × 108 CFU per mL, respectively, mixed in the daily quota of feed. The control group (CON) was fed a basal diet without azolla and DFM. Azolla fed alone and with DFM did not affect growth performance and feed conversion ratio (FCR) of birds during pre-starter and starter phase; however, significantly (P < 0.05) lower weight gain and higher FCR were observed during finisher period, resulting in an impaired overall FCR. Decreased (P < 0.05) plasma cholesterol and increased (P < 0.05) tibial calcium content were observed in birds fed azolla with DFM. The highest value (P < 0.05) of carcass characteristics like gizzard weight, breast muscle yield and lower meat pH were recorded in the birds fed azolla in combination with DFM, followed by birds fed azolla alone. In conclusion, azolla feeding at 2.5% level shows positive effect on breast muscle yield and gizzard weight of broiler which can be enhanced upon adding DFM to azolla-based diet.