Transcriptomic responses and apoptosis in larval red drum (Sciaenops ocellatus) co-exposed to crude oil and ultraviolet (UV) radiation.

Ultraviolet (UV) radiation can significantly increase the toxicity of polycyclic aromatic hydrocarbons (PAHs) in crude oil to early life stage (ELS) fishes through photo-induced /photo-enhanced toxicity. However, little is known about the sub-lethal effects and mechanisms of photo-induced PAH toxicity in ELS fishes. The present study investigated apoptosis and global transcriptomic effects in larval red drum (Sciaenops ocellatus) (24-72 h post-fertilization) following co-exposure to oil (0.29-0.30 µg/L ∑PAH50) and UV. Apoptosis was quantified using the TUNEL assay, and transcriptomic effects were assessed using RNA sequencing analysis. Apoptotic fluorescence was greatest in the eyes and skin following 24 and 48 h co-exposure to oil and UV, indicating photo-induced toxicity. Consistent with these phenotypic responses, pathways associated with phototransduction, eye development, and dermatological disease were among the top predicted pathways impacted. The present study is the first to provide global transcriptomic analysis of UV and oil co-exposure in an ELS fish.

Influence of variable ultraviolet radiation and oil exposure duration on survival of red drum (Sciaenops ocellatus) larvae.

The toxicity of some polycyclic aromatic hydrocarbons (PAHs) increases with ultraviolet (UV) radiation. The intensity of UV radiation varies within aquatic ecosystems, potentially providing reprieves during which tissue repair may occur. Transient/short-term PAH exposure prior to UV exposure may initiate metabolism/clearance, potentially affecting outcomes. Larval Sciaenops ocellatus were exposed to oil and UV radiation, using either variable photoperiods or pre-UV oil exposure durations. Shorter PAH exposures exhibited greater toxicity, as did exposure to shorter photoperiods. Environ Toxicol Chem 2018;37:2372-2379. © 2018 SETAC.