ABSTRACT
Biosilicate is a bioactive glass-ceramic used in medical and dental applications. This study evaluated novel reparative materials composed of pure tricalcium silicate (TCS), 30% zirconium oxide (ZrO2 ) and 10 or 20% biosilicate, in comparison with Biodentine. Setting time was evaluated based on ISO 6876 standard, radiopacity by radiographic analysis, solubility by mass loss, and pH by using a pH meter. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and NR assays. Alkaline phosphatase (ALP) activity and alizarin red were used to evaluate cell bioactivity. Antimicrobial activity was assessed on Enterococcus faecalis by the direct contact test. The data were submitted to analysis of variance (ANOVA)/Tukey; Bonferroni and Kruskal-Wallis, and Dunn tests (α = 0.05). The association of Biosilicate with TCS + ZrO2 had appropriate setting time, radiopacity, and solubility, alkaline pH, and antimicrobial activity. TCS and Biodentine showed higher ALP activity in 14 days than the control (serum-free medium). All cements produced mineralized nodules. In conclusion, Biosilicate + TCS ZrO2 decreased the setting time and increased the radiopacity in comparison to TCS. Biosilicate + TCS ZrO2 presented lower solubility and higher radiopacity than Biodentine. In addition, these experimental cements promoted antimicrobial activity and mineralization nodules formation, suggesting their potential for clinical use.
Subject(s)
Calcium Compounds/chemistry , Glass/chemistry , Silicates/chemistry , Zirconium/chemistry , Alkaline Phosphatase , Anthraquinones , Biocompatible Materials , Bone Cements , Bone Neoplasms/pathology , Calcium Compounds/pharmacology , Calcium Compounds/toxicity , Cell Line, Tumor , Dental Cements , Enterococcus faecalis/drug effects , Humans , Hydrogen-Ion Concentration , Materials Testing , Osteosarcoma/pathology , Silicate Cement , Silicates/pharmacology , Silicates/toxicity , Solubility , Zirconium/pharmacology , Zirconium/toxicityABSTRACT
Abstract Differences in liquid-to-powder ratio can affect the properties of calcium silicate-based materials. This study assessed the influence of powder-to-gel ratio on physicochemical properties of NeoMTA Plus. Setting time (minutes), flow (mm and mm²), pH (at different periods), radiopacity (mm Al) and solubility (% mass loss) were evaluated using the consistencies for root repair material (NMTAP-RP; 3 scoops of powder to 2 drops of gel) and root canal sealer (NMTAP-SE; 3 scoops of powder to 3 drops of gel), in comparison to Biodentine cement (BIO) and TotalFill BC sealer (TFBC). Statistical analysis was performed using one-way ANOVA and Tukey tests (α=0.05). BIO had the shortest setting time, followed by NMTAP-RP and NMTAP-SE. TFBC showed the highest setting time and radiopacity. BIO, NMTAP-RP, and NMTAP-SE had similar radiopacity. All materials promoted an alkaline pH. NMTAP-RP/SE presented lower solubility than BIO and TFBC. Regarding the flow, TFBC had the highest values, followed by NMTAP-SE, and NMTAP-RP. BIO had the lowest flow. In conclusion, NMTAP in both powder-to-gel ratios showed high pH and low solubility. The increase in the powder ratio decreased the setting time and flow. These findings are important regarding the proper consistency and work time to clinical application.
Resumen Las diferencias en la proporción líquido/polvo pueden afectar las propiedades de los materiales a base de silicato de calcio. Este estudio evaluó la influencia de la proporción polvo/gel en las propiedades fisicoquímicas del cemento NeoMTA Plus. El tiempo de fraguado (minutos), la fluidez (mm y mm²), el pH (en diferentes períodos), la radiopacidad (mmAl) y la solubilidad (% de pérdida de masa) fueron evaluados utilizando las consistencias para el material de reparación radicular (NMTAP-RP; 3 cucharadas de polvo/2 gotas de gel) y para cemento sellador del conducto radicular (NMTAP-SE; 3 cucharadas de polvo/3 gotas de gel), en comparación con el cemento Biodentine (BIO) y el cemento TotalFill BC (TFBC). El análisis estadístico se realizó utilizando las pruebas ANOVA y Tukey unidireccionales (α=0.05). BIO tuvo el tiempo de fraguado más corto, seguido de NMTAP-RP y NMTAP-SE. TFBC mostró el mayor tiempo de fraguado y radiopacidad. BIO, NMTAP-RP y NMTAP-SE tuvieron una radiopacidad similar. Todos los materiales promovieron un pH alcalino. NMTAP-RP/ SE tuvieron una solubilidad menor que BIO y TFBC. Con respecto a la fluidez, TFBC tuvo los valores más altos, seguido de NMTAP-SE y NMTAP-RP. BIO tuvo la fluidez más baja. En conclusión, NMTAP en la relación polvo/gel mostró un pH alto y una baja solubilidad. El aumento en la proporción de polvo disminuyó el tiempo de fraguado y la fluidez. Estos hallazgos son importantes con respecto a su consistencia y tiempo de trabajo durante la aplicación clínica.
Subject(s)
Calcarea Silicata/analysis , Chemistry, Physical , Dental CementumABSTRACT
OBJECTIVE: To evaluate the cytotoxicity, genotoxicity and antibacterial activity of poly(vinyl alcohol)-coated silver nanoparticles (AgNPs-PVA) and farnesol (FAR). DESIGN: The cytotoxicity (% of cell viability) was evaluated by MTT assay and the genotoxicity (% of DNA in the tail) was evaluated by Comet assay. Root canal disinfection with different irrigating protocols was evaluated ex vivo in human teeth contaminated with Enterococcus faecalis for 21days. Three microbiological samples were collected: initial (after contamination); post-irrigation (after irrigation); and final (after 7days). After each sample, the number of log 10 CFU mL-1 was determined. Statistical analyses was performed using two-way ANOVA and Bonferroni post-hoc tests for MTT assay, Kruskal-Wallis and Dunn post-hoc tests for Cometa and antibacterial assays (α=0.05). RESULTS: The MTT assay showed that AgNPs and FAR were less cytotoxic that sodium hypochlorite (NaOCl) and showed a lower% of DNA in the tail, in comparison with H2O2 (positive control - C+). In the post-irrigation microbiological sample, all the irrigating protocols were more effective than C+ (without irrigation). NaOCl/saline, NaOCl/saline/AgNPs-PVA and NaOCl/saline/FAR led to complete bacterial elimination (p >0.05). In comparison with the initial sample, both the post-irrigation and the final samples showed microbial reduction (p < 0.05). CONCLUSIONS: AgNPs-PVA and FAR showed low cytotoxicity and genotoxicity, and exhibit potential for use as a final endodontic irrigation protocols.
Subject(s)
Anti-Bacterial Agents/pharmacology , Coated Materials, Biocompatible/pharmacology , Enterococcus faecalis/drug effects , Farnesol/pharmacology , Metal Nanoparticles , Polyvinyl Alcohol/pharmacology , Root Canal Irrigants/pharmacology , Silver/pharmacology , Cell Survival/drug effects , Cells, Cultured , Colony Count, Microbial , Comet Assay , Disinfection , Fibroblasts/drug effects , Humans , In Vitro Techniques , Mutagenicity Tests , Sodium Hypochlorite/pharmacologyABSTRACT
La cirugía perirradicular está indicada en casos de fracaso del tratamiento endodóntico convencional y/o cuando no es posible realizarlo. Entre las modalidades existentes, la obturación retrógrada consiste en preparar y obturar la cavidad apical con materiales llamados de retroobturadores. La evolución técnica y científica de la endodoncia posibilita la utilización de puntas ultrasónicas, las cuales han facilitado la confección de retrocavidades con extensión adecuada y siguiendo el eje longitudinal de la raíz. El objetivo del presente estudio es presentar los recursos actuales respecto a la preparación de la retrocavidad en la cirugía perirradicular, destacando sus ventajas y comparándola con el empleo de la preparación convencional con el uso de fresas. Será presentada una revisión de la literatura, considerándose el empleo de ultrasonido y láser para preparación retrógrada, buscando recursos actuales que ofrezcan una mejor preparación apical y por consiguiente, mejorando el pronóstico de las cirugías periapicales (AU)
The endodontic surgery is indicated in cases of failure and/or impossibility of conventional endodontic treatment. Among the existent modalities in the endodontic surgery, the retrograde filling consists on preparation of apical cavity and filling with a retrofilling material. The technical-scientific progress in Endodontics become possible the use of retrotips activated with ultrasonic for retrocavities preparation with appropriate extension and following the long axis of the root. The aim of the study was to present the current resources in the preparation of the retrograde cavity in endodontic surgery, detaching their advantages in comparison with the use of conventional preparation with burns. A review of the literature will be presented, considering the use of ultrasound and laser for retrograde preparation, seeking the current resources that provide better apical preparation, resulting in better prognostic of the endodontic surgery (AU)
Subject(s)
Humans , Retrograde Obturation/methods , Root Canal Preparation/methods , Root Canal Obturation/methods , Laser Therapy/methods , Ultrasonic Therapy/methodsABSTRACT
La rehabilitación oral con implantes osteointegrados es un tratamiento que está bien documentado y con un alto porcentaje de éxito. Sin embargo, exigencias relacionadas al tiempo de tratamiento y a la técnica quirúrgica fueron surgiendo. En ese contexto, el procedimiento de carga inmediata, en el cual la prótesis dental es instalada inmediatamente después del procedimiento quirúrgico se tornó una práctica constante. A pesar de que la carga inmediata ha sido un importante avance, procedimientos mínimamente invasivos, como la instalación de implantes con la técnica directa, sin desplazamiento del colgajo mucoperiostio (flapless) vienen siendo cada vez más realizados. La asociación de la carga inmediata con la técnica directa o de flapless mejora la aceptación de los pacientes y profesionales, pues no hay necesidad de sutura, además de reducir el edema, la hemorragia durante y después de la cirugía, el dolor post-operatorio, el tiempo quirúrgico, la incomodidad y hematoma, reduciendo también la necesidad de medicación post-operatoria. Esas características facilitan las etapas de rehabilitación inmediatamente después de la colocación de los implantes, colaborando con el trabajo del técnico protésico. De esa forma, la propuesta de este trabajo es presentar un caso clínico de rehabilitación oral con implantes osteointegrados y prótesis fijas en ambas arcadas, donde se realizó la técnica directa seguida por carga inmediata, relatando el diagnóstico, planificación, procedimientos quirúrgico/protésicos y seguimiento de 20 meses
The oral rehabilitation with osseointegrated implants is a well-documented treatment with high rate of success. Nevertheless, demands related to treatment time and surgical technique began to appear. In this context, the procedure of immediate loading in which the dental prosthesis is placed right after implant surgery has become a constant practice. Although immediate loading has been an important advance, minimally invasive procedures, such as implant placement without raising a mucoperiosteal flap (flapless) are increasingly being performed. Association of immediate loading with the flapless technique improves acceptance by patients and professionals, because no suturing is required. Moreover, it reduces swelling, bleeding during and after surgery, postoperative pain, surgery time, discomfort and hematoma, as well as the need for postoperative medication. These characteristics ease the stages of rehabilitation soon after implant placement, cooperating with prosthodontist's work. Thus, the proposal of this study is to present a clinical case of oral rehabilitation with osseointegrated implants and fixed prosthesis in both arches, in which the flapless technique was applied, followed by immediate loading. It will discuss the diagnosis, prosthetic planning, surgical/prosthodontic procedures and follow-up for 20 months
Subject(s)
Humans , Female , Middle Aged , Immediate Dental Implant Loading/methods , Mouth Rehabilitation , Dental Prosthesis/methods , Surgery, OralABSTRACT
El objetivo de este estudio fue evaluar el comportamiento biológico de los cementos endodónticos Endofill, Endomethasone y Sealer 26, en tejido conjuntivo subcutáneo de ratones. Fueron establecidos tres grupos experimentales y un grupo control de cinco animales cada uno, en los períodos de 7, 21 y 60 días. Los cortes histológicos obtenidos fueron coloreados con Hematoxilina & Eosina y con tricrómico de Masson, para posteriormente ser evaluadas la reacción inflamatoria adyacente y la formación de tejido fibroso. Los datos obtenidos fueron sometidos al test estadístico de Kruskall-Wallis y Miller con 5% de nivel de significancia. El análisis microscópico mostró reacción inflamatoria más intensa en los períodos de 7 y 21días. A los 60 días hubo formación de una cápsula fibrosa densa para el cemento Endomethasone y parcialmente densa para los demás cementos. El análisis estadístico mostró diferencia estadísticamente significativa con mayor intensidad de reacción inflamatoria solamente para el grupo II (Endofill) en el período de 60 días en relación a los grupos I (control) y grupo III (Endomethasone). Todos los materiales presentaron reacción inflamatoria más intensa en los períodos inicial e intermediario, siendo que la Endomethasone presentó los mejores resultados.
The aim of this study was to evaluate the biological behavior of the root canal filling sealers: Endofill, Endomethasone and Sealer 26 when in contact with the subcutaneous connective tissue of rat. For the study one control and three experimental groups were used. A total of 15 animals were divided into 5 for each period: 7, 21, and 60 days. The obtained histological sections were processed and stained using the hematoxiline & eosine technique. The histological sections were subjective and comparatively analyzed using optic microscopy. The intensity of the inflammatory reaction and the level of fibrosis of the tissue were registered. The results were registered in scores and statistical analysis by KRUSKAL-WALLIS p<0,05 and MILLER methods. The statistical analysis revealed that in the period of 60 days, there was statistical significance to group II (Endofill) between group (control) and III (Endomethasone) with mononuclear cells into connective tissue. All materials promoted inflammatory reaction more intense at 7 and 21 days with the Endomethasone showing the best results.
Subject(s)
Animals , Mice , Dental Pulp Cavity/injuries , Dental Cements/analysis , Materials Testing/methodsABSTRACT
This study evaluated the marginal sealing ability of six temporary restorative materials. Seventy-six human premolars were extracted and divided into six groups (n = 12); the remaining four teeth were used as a control group. Coronal access and biomechanical preparation were performed and the root canals were sealed. The external dental surfaces were covered and the coronal access cavities were filled with one of the six materials. At that point, the teeth were immersed in 0.2% Rhodamine B solution for 72 hours and subjected to thermal cycling. Samples were rinsed in running water and sectioned longitudinally; at that point, the images of each tooth were digitized and marginal leakage was measured using the Image Tool program. Data were subjected to statistical analysis by ANOVA and Tukey's test for comparison between experimental groups. All of the glass ionomer cements tested, particularly Maxxion R, offered satisfactory sealing ability as temporary restorative materials.
