High-intensity Focused Ultrasound-A New Choice to Conduct Pulmonary Artery Denervation.

This research aimed to explore whether high-intensity focused ultrasound (HIFU) could conduct pulmonary artery denervation (PADN). HIFU was performed in pulmonary arteries of 6 normotensive rabbits at dose of 250W, 6 times for each rabbit, and an additional 6 rabbits served as controls. Then ATEPH was induced in both groups by intravenous infusion of autogeneic thrombus. Hemodynamics and ultrasonography parameters were measured by right heart catheter and echocardiography pre- and post-establishment of ATEPH models in both groups. Histological analysis and immunohistochemistry of tyrosine hydroxylase (TH) were also performed. After PADN procedures, 5 rabbits were successfully conducted PADN, of which ablation zone was also observed in right auricle or right lung in 4 rabbits. Ablation zone was detected only in right lung in 1 rabbit. Compared with control group, milder right heart hemodynamic changes were found in PADN group, accompanied by improved ultrasound parameters in PADN group. HIFU can acutly damage SNs around pulmonary artery successfully, which may be a new choice to conduct PADN. However, the accuracy of HIFU with PADN needs to be improved.

Lactylation: novel epigenetic regulatory and therapeutic opportunities.

Lactate, which is an end product of glycolysis, has traditionally been considered a metabolic waste. However, numerous studies have demonstrated that lactate serves metabolic and nonmetabolic functions in physiological processes and multiple diseases. Cancer and pulmonary arterial hypertension have been shown to undergo metabolic reprogramming, which is accompanied by increased lactate production. Metabolic reprogramming and epigenetic modifications have been extensively linked; furthermore, posttranslational modifications of histones caused by metabolites play a vital role in epigenetic alterations. In this paper, we reviewed recent research on lactate-induced histone modifications and provided a new vision about the metabolic effect of glycolysis. Based on our review, the cross talk between the metabolome and epigenome induced by glycolysis may indicate novel epigenetic regulatory and therapeutic opportunities. There is a magnificent progress in the interaction between metabolomics and epigenomics in recent decades, but many questions still remained to be investigated. Lactylation is found in different pathophysiological states and leads to diverse biological effects; however, only a few mechanisms of lactylation have been illustrated. Further research on lactylation would provide us with a better understanding of the cross talk between metabolomics and epigenomics.

Progress of Pathogenesis in Pediatric Multifocal Atrial Tachycardia.

Multifocal atrial tachycardia (MAT) is defined as irregular P-P, R-R, and P-R intervals, isoelectric baseline between P waves, and ventricular rate over 100 beats/min. Although the prognosis of pediatric MAT in most patients is favorable, adverse outcomes of MAT have been reported, such as cardiogenic death (3%), respiratory failure (6%), or persistent arrhythmia (7%), due to delayed diagnosis and poorly controlled MAT. Previous studies demonstrated that pediatric MAT is associated with multiple enhanced automatic lesions located in the atrium or abnormal automaticity of a single lesion located in the pulmonary veins via multiple pathways to trigger electrical activity. Recent studies indicated that pediatric MAT is associated with the formation of a re-entry loop, abnormal automaticity, and triggering activity. The occurrence of pediatric MAT is affected by gestational disease, congenital heart disease, post-cardiac surgery, pulmonary hypertension, and infectious diseases, which promote MAT via inflammation, redistribution of the autonomic nervous system, and abnormal ion channels. However, the pathogenesis of MAT needs to be explored. This review is aimed to summarize and analyze the pathogenesis in pediatric MAT.

Morphology-Dependent Electrocatalytic Performance of a Two-Dimensional Nickel-Iron MOF for Oxygen Evolution Reaction.

Developing highly efficient, low-cost, and durable oxygen evolution reaction (OER) electrocatalysts is extraordinarily desirable for achieving clean and sustainable hydrogen energy. Metal-organic frameworks (MOFs) are emerging as attractive candidates for OER electrocatalysts. Herein, a two-dimensional Fe-Ni MOF of Fe(py)2Ni(CN)4 (py = pyridine) is synthesized controllably to generate various nanostructures, including nanoboxes, nanocubes, nanoplates, and nanosheets. Since different morphologies expose different active crystal planes and generate disparate intrinsic active sites, these nanostructures exhibit obviously different electrocatalytic activities. Particularly, the nanoboxes with a hollow structure display superior electrocatalytic activity and stability for OER due to greater active surface area and higher intrinsic activity of the exposed crystal planes, delivering a low overpotential of 285 mV at 10 mA cm-2 and a small Tafel value of 50.9 mV dec-1 in a 1.0 M KOH solution. The morphology-dependent electrocatalytic properties demonstrated in this work provide an efficient strategy to optimize MOF precatalysts for electrochemical energy storage and conversion.

Construction of rGO-Encapsulated Co3 O4 -CoFe2 O4 Composites with a Double-Buffer Structure for High-Performance Lithium Storage.

Transition metal oxides (TMOs) are promising anode materials for next-generation lithium-ion batteries (LIBs). Nevertheless, their poor electronic and ionic conductivity as well as huge volume change leads to low capacity release and rapid capacity decay. Herein, a reduced graphene oxide (rGO)-encapsulated TMOs strategy is developed to address the above problems. The Co3 O4 -CoFe2 O4 @rGO composites with rGO sheets-encapsulated Co3 O4 -CoFe2 O4 microcubes are successfully constructed through a simple metal-organic frameworks precursor route, in which Co[Fe(CN)5 NO] microcubes are in situ coated by graphene oxide sheets, followed by a two-step calcination process. As anode material of LIBs, Co3 O4 -CoFe2 O4 @rGO exhibits remarkable reversible capacity (1393 mAh g-1 at 0.2 A g-1 after 300 cycles), outstanding long-term cycling stability (701 mAh g-1 at 2.0 A g-1 after 500 cycles), and excellent rate capability (420 mAh g-1 at 4.0 A g-1 ). The superior lithium storage performance can be attributed to the unique double-buffer structure, in which the outer flexible rGO shells can prevent the structure collapse of the electrode and improve its conductivity, while the hierarchical porous cores of Co3 O4 -CoFe2 O4 microcubes can buffer the volume expansion. This work provides a general and straightforward strategy for the construction of novel rGO-encapsulated bimetal oxides for energy storage and conversion application.

Size-controllable synthesis of Zn2GeO4 hollow rods supported on reduced graphene oxide as high-capacity anode for lithium-ion batteries.

Germanium-based ternary oxides have aroused wide attention as an anode for high-performance lithium-ion batteries (LIBs). Nevertheless, they usually suffer a large volume expansion and rapid capacity fading during lithiation/delithiation cycles. To address this issue, herein, Zn2GeO4/RGO composites are synthesized with Zn2GeO4 hollow rods in-situ grown on reduced graphene oxide (RGO) sheets. The Zn2GeO4 hollow rods can be facilely adjusted from nano- to micro-size. The lithium storage performances of the composites strongly depend on the size of Zn2GeO4 hollow rods and the content of RGO. The optimized Zn2GeO4/RGO composite exhibits a pseudocapacitance-dominated Li+ storage performance, with a large reversible capacity of 1005 mAh g-1 after 100 cycles at 0.5 A g-1, an excellent rate capability (515 mAh g-1 at a high rate of 5 A g-1) and a good long cycling stability of 500 cycles with a low capacity loss of 0.05% per cycle at 1 A g-1. The outstanding electrochemical performance can be attributed to the unique composition and microstructure of the material as well as the synergistic effect of the conductive RGO sheets and the hollow Zn2GeO4 nanostructure. This work provides a promising anode for high-performance LIBs and a useful inspiration for further improving the Ge-based ternary oxide anodes.

Facile synthesis of novel tungsten-based hierarchical core-shell composite for ultrahigh volumetric lithium storage.

The development of novel high volumetric capacity electrode materials is crucial to the application of lithium-ion batteries (LIBs) in miniaturized consumer electronics. In this work, a novel tungsten-based octahedron (CoWO4/Co3O4) with unique hierarchical core-shell structure is successfully fabricated by simply calcinating a cyanide-metal framework precursor. Benefitting from the heavy element W, the CoWO4/Co3O4 octahedrons show a high mass density of 5.18 g cm-3. When applied as anode materials for LIBs, the CoWO4/Co3O4 octahedrons exhibit an ultrahigh volumetric capacity (6226 mAh cm-3 after 350 cycles at 0.4 A g-1), superior rate capability (3165 mAh cm-3 at 3.0 A g-1) and outstanding long-term cycling performance (4703 mAh cm-3 at 1.0 A g-1 after 800 cycles). The extraordinary lithium storage performance can be ascribed to the unique hierarchical core-shell structure and the possible synergistic effect between W and Co, which provide more Li+ insertion sites and effectively buffer the volume variation during cycling. This work not only provides an ultrahigh volumetric lithium storage anode, but also gives a simple and general strategy for the synthesis of novel anode materials for high volumetric energy density LIBs.

Sequence features accurately predict genome-wide MeCP2 binding in vivo.

Methyl-CpG binding protein 2 (MeCP2) is critical for proper brain development and expressed at near-histone levels in neurons, but the mechanism of its genomic localization remains poorly understood. Using high-resolution MeCP2-binding data, we show that DNA sequence features alone can predict binding with 88% accuracy. Integrating MeCP2 binding and DNA methylation in a probabilistic graphical model, we demonstrate that previously reported genome-wide association with methylation is in part due to MeCP2's affinity to GC-rich chromatin, a result replicated using published data. Furthermore, MeCP2 co-localizes with nucleosomes. Finally, MeCP2 binding downstream of promoters correlates with increased expression in Mecp2-deficient neurons.

Lentivirus-mediated genetic manipulation and visualization of olfactory sensory neurons in vivo.

Development of a precise olfactory circuit relies on accurate projection of olfactory sensory neuron (OSN) axons to their synaptic targets in the olfactory bulb (OB). The molecular mechanisms of OSN axon growth and targeting are not well understood. Manipulating gene expression and subsequent visualizing of single OSN axons and their terminal arbor morphology have thus far been challenging. To study gene function at the single cell level within a specified time frame, we developed a lentiviral based technique to manipulate gene expression in OSNs in vivo. Lentiviral particles are delivered to OSNs by microinjection into the olfactory epithelium (OE). Expression cassettes are then permanently integrated into the genome of transduced OSNs. Green fluorescent protein expression identifies infected OSNs and outlines their entire morphology, including the axon terminal arbor. Due to the short turnaround time between microinjection and reporter detection, gene function studies can be focused within a very narrow period of development. With this method, we have detected GFP expression within as few as three days and as long as three months following injection. We have achieved both over-expression and shRNA mediated knock-down by lentiviral microinjection. This method provides detailed morphologies of OSN cell bodies and axons at the single cell level in vivo, and thus allows characterization of candidate gene function during olfactory development.

Olfactory sensory axon growth and branching is influenced by sonic hedgehog.

Olfactory sensory neuron (OSN) axons extend from the olfactory epithelium to the olfactory bulb without branching until they reach their target region, the glomerulus. In this report, we present evidence to support the involvement of sonic hedgehog in promoting rat olfactory sensory axons to branch and to enter into the glomerulus. Sonic hedgehog (Shh) protein is detected in the glomeruli of the olfactory bulb, whereas its transcript is expressed in the mitral and tufted cells, suggesting that Shh in the glomeruli is produced by mitral and tufted cells. In primary OSN cultures, Shh-N peptide promotes olfactory axon branching. When Shh function is neutralized in vivo by its antibody, growth of newly generated OSN axons into the glomeruli is vastly reduced.