Camellia oil exhibits anti-fatigue property by modulating antioxidant capacity, muscle fiber, and gut microbial composition in mice.

Camellia seed oil (CO) has high nutritional value and multiple bioactivities. However, the specific anti-fatigue characteristics and the implied mechanism of CO have not yet been fully elucidated. Throughout this investigation, male C57BL/6J mice, aged 8 weeks, underwent exhaustive exercise with or without CO pretreatment (2, 4, and 6 mL/kg BW) for 28 days. CO could extend the rota-rod and running time, reduce blood urea nitrogen levels and serum lactic acid, and increase muscle and hepatic glycogen, adenosine triphosphate, and anti-oxidative indicators. Additionally, CO could upregulate the mRNA and Nrf2 protein expression levels, as well as enhance the levels of its downstream antioxidant enzymes and induce the myofiber-type transformation from fast to slow and attenuate the gut mechanical barrier. Moreover, CO could ameliorate gut dysbiosis by reducing Firmicutes to Bacteroidetes ratio at the phylum level, increasing the percentage of Alistipes, Alloprevotella, Lactobacillus, and Muribaculaceae, and decreasing the proportion of Dubosiella at the genus level. In addition, specific bacterial taxa, which were altered by CO, showed a significant correlation with partial fatigue-related parameters. These findings suggest that CO may alleviate fatigue by regulating antioxidant capacity, muscle fiber transformation, gut mechanical barrier, and gut microbial composition in mice. PRACTICAL APPLICATION: Our study revealed that camellia seed oil (CO) could ameliorate exercise-induced fatigue in mice by modulating antioxidant capacity, muscle fiber, and gut microbial composition in mice. Our results promote the application of CO as an anti-fatigue functional food that targets oxidative stress, myofiber-type transformation, and microbial community.

[Clinical using and observation of occlusion splint of RPD].

PURPOSE: This article describes the design and restoration for 180 cases diagnosed with dentition defect accompanied with medium to severe abrasion and attrition. The purpose of this study is to discuss the rehabilitation of vertical dimension, the balance of occlusion and the whole occlusion system. METHODS: Before clinical preparation of the 180 clinical cases, diagnostic casts were made. Entire restoration treatment plan was adopted to make removable partial dentures with full crown restorations along with occlusion splints. The clinical effect were evaluated at 2nd week, 4th week and 9th week. RESULTS: Of the 180 cases, 148 patients (82.2%) adapted to the denture in 2-3 weeks, 24 patients (13.3%) in 4-5 weeks, 8 patients (14.5%) in 5-6 weeks. The further observation were evaluated over 10-15 years with clinical satisfaction. CONCLUSION: This method not only reserves the tooth structure, but also restores configuration and function of the lost tissues. It's really a constantly effective remedy.