Magnetic Resonance Imaging Artifact Elimination in the Diagnosis of Female Pelvic Abscess under Phase Correction Algorithm.

In order to explore the effect of magnetic resonance imaging (MRI) based on phase correction algorithm in diagnosing female pelvic abscess, firstly, the effect of phase correction algorithm on eliminating MRI image motion artifacts was studied, then it was applied to 71 female pelvic cases admitted to our hospital in the diagnosis of abscess patients with magnetic resonance imaging technology, and the results were compared with the results of multislice spiral CT and laparoscopy to explore the accuracy of MRI and CT. It was found that the results of MRI examination were close to those of laparoscopy, and the difference was not statistically significant (P > 0.05); the results of CT examination and laparoscopy were significantly different, and the difference was statistically significant (P < 0.05); in addition, the results of CT examination, the number of bacterial cysts (43 cases) and tuberculous cysts (12 cases), were significantly lower than the results of MRI (50 cases, 18 cases), and the difference was statistically significant (P < 0.05). The size of the mass shown by the MRI examination (4.1 cm × 4.2 cm × 3.9 cm～13.9 cm × 9.5 cm × 8.7 cm) was larger than the size of the mass revealed by the CT examination (5.2 cm × 4.3 cm × 4.1 cm～15.5 cm × 10.1 cm × 9.6 cm), the difference between the two was statistically significant (P < 0.05), and it was closer to the results of laparoscopic pathology (4.1 cm × 4.3 cm × 3.9 cm～14.1 cm × 9.3 cmP < 0.058.7 cm). In short, the phase correction algorithm could eliminate the motion artifacts of MRI images. In the imaging diagnosis of female pelvic abscess, the MRI diagnosis based on the phase correction algorithm is more ideal than the diagnosis of multislice spiral CT. It can be used as a reference basis for clinical disease treatment.

HOXD-AS1 facilitates cell migration and invasion as an oncogenic lncRNA by competitively binding to miR-877-3p and upregulating FGF2 in human cervical cancer.

BACKGROUND: Long non-coding RNAs (LncRNAs) are dysregulated in multiple human cancers and they are highly involved in tumor progression. Previous studies have identified the oncogenic lncRNA HOXD cluster antisense RNA 1 (HOXD-AS1) in human cancers, while its roles in cervical cancer (CC) remain unclear. Herein we intended to characterize the implication of HOXD-AS1 in CC. METHODS: qRT-PCR was applied to examine the relative expression of HOXD-AS1 in CC tissues, cell lines and transfected cells. Wound healing and transwell assays were applied to detect cell migration and invasion alteration. The targeting relationship between miRNA and mRNA/lncRNA was determined by dual luciferase reporter, qRT-PCR and western blot assays. RESULTS: HOXD-AS1 was overexpressed in CC tissues and cell lines. Its higher level predicted worse prognosis of CC patients. SiRNA mediated knockdown of HOXD-AS1 repressed CC cell migration and invasion, and its overexpression did the opposite. Mechanistically, HOXD-AS1 acted as a competing endogenous RNA (ceRNA) to sponge miR-877-3p and led to upregulation of FGF2, a target of miR-877-3p. Importantly, either miR-877-3p overexpression or FGF2 inhibition could abolish the migration and invasion promotion induced by HOXD-AS1. CONCLUSION: HOXD-AS1 functions as a tumor-promoting lncRNA via the miR-877-3p/FGF2 axis in CC. HOXD-AS1 might be a promising therapeutic target as well as a novel prognostic biomarker for CC.

Gammacerane suppresses the growth of human endometrial carcinoma cells via induction of apoptosis, cell cycle arrest, inhibition of cell migration, invasion and STAT3 signaling pathway.

PURPOSE: This study was conducted to assess the anticancer role of gammacerane against human endometrial cancer. METHODS: The human RL-95 cell line (endometrial cancer) and SV40 (normal endometrial cells) were used in this study. The MTT-based estimation of cell proliferation assay along with the colony formation assay were used for assessing the cell viability. Acridine orange (AO)/Ethidium bromide (EB) staining followed by fluorescent microscopy was performed for estimation of cell apoptosis. Flow cytometry was used to assess the cell cycle phase distribution of cancer cells. Cell migration and invasion were estimated using wound healing and transwell assay, respectively. Western blotting was used for protein expression studies. RESULTS: The cell proliferation assay revealed that gammacerane treatment led to loss of viability of RL-95 cancer cells in a concentration-dependent manner. However, the antiproliferative effects were comparatively less prominent when gammacerane was used against the SV40 normal endometrial cells. AO/EB staining of cancer cells showed that gammacerane is active in inducing apoptosis in RL-95 cells and apoptotic induction effects were more evident at higher concentrations of the molecule. Flow cytometric analysis with Annexin V-FITC/Propidium iodide (PI) fixed cells showed that the percentage of apoptotic cells increased with increase in gammacerane concentration. Apoptotic signal was mediated via the modulation of Bax/Bcl-2 protein ratio. Western blot analysis of STAT3 protein showed that gammacerane treatment reduced the protein levels of STAT3 and the effects were more prominent at higher treatment concentrations. CONCLUSION: Gammacerane, by its ability to take control over the transcription of STAT3 transcription factor, inhibits the proliferation of human endometrial cancer cells. The effects revealed loss of viability, arrest of mitosis and cellular apoptosis.

Aptamer (AS1411)-Conjugated Liposome for Enhanced Therapeutic Efficacy of miRNA-29b in Ovarian Cancer.

AS1411 Aptamer-functionalized liposome was successfully formulated and found to be nanosized. Flow cytometer and CLSM results demonstrated that Aptamer enhanced the targeting of carrier in the cancer cells via nucleolin-mediated transmembrane endocytosis pathway. The lipofectaminebased miR-29b showed a typical concentration-dependent cytotoxic effect in the cancer cells. LP-miR induced a significant reduction in the cell viability of A2780 cells compared to that of nontreated control, while LP-Mut (mutant loaded) did not have any effect on the cell viability indicating the importance of the specific gene sequencing. LP-miR induced a significant decrease in the green fluorescence which is indicative of the decrease in the cell viability. Simultaneously, higher PI positive cells were observed for LP-miR treated cancer cells in Live/Dead assay. Cells treated with LP-miR exhibited the brightest fluorescence indicating the presence of apoptotic cells. Significant increase in the Annexin-V+ cells and PI+ cells were observed for cell treated with LP-miR compared to that of non-treated control indicating the potential of miR-29b. This novel miR-29b-loaded Aptamer-directed liposome could potential serve as a new platform to improve the therapeutic outcome in ovarian cancers.

[Effects of Gui Zhi Fu Ling Jiao Nang on Hemorheology and Expression of Phenotypic Proteins in Uterine Smooth Muscle Cells in Rats with An Intrauterine Device].

Objective To observe the effect of Gui Zhi Fu Ling Jiao Nang (GZFLJN) on the expressions of alpha smooth muscle actin (α-SMA) and proliferating cell nuclear antigen (PCNA) in uterine vascular smooth muscle cells (VSMC) of rat models with an intrauterine device (IUD) and to determine the thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) levels in peripheral blood. Methods Female Wistar rats were randomly divided into four groups:normal group (n=16,with normal breed without treatment),model group (n=18,drenching 0.9% normal saline after modeling of IUD),GZFLJN group (n=18),and aminocaproic acid tablets group (n=17). Immunohistochemical SP method was used to detect the expressions of α-SMA and PCNA in uterine VSMC.ELISA was served to detect the levels of TXB2 and 6-keto-PGF1α in peripheral blood. Results The positive rate of α-SMA were (50.89±9.41)%,(26.93±6.80)%,(48.92±6.80)%,and (34.63±7.26)%,respectively,in normal group,model group,GZFLJN group,and aminocaproic acid tablets group;obviously,it was significantly higher in normal group (t=14.43,P=0.00) and GZFLJN group (t=11.37,P=0.00) than that in model group and it was significantly lower in aminocaproic acid tablets group than in normal group (t=9.96,P=0.00) and GZFLJN group (t=8.23,P=0.00). The positive rate of PCNA were (25.66±7.24)%,(61.26±9.98)%,(28.36±9.17)%,and (50.23±8.71)%,respectively,in these four groups;obviously,it was significantly lower in the normal group (t=20.86,P=0.00) and GZFLJN group (t=19.12,P=0.00) than in model group and it was significantly higher in aminocaproic acid tablets group than in normal group (t=17.82,P=0.00) and GZFLJN group (t=16.05,P=0.00). Serum TXB2 level in these four groups were (445.86±24.43),(508.78±12.42),(448.11±9.63),and (498.11±13.63)ng/L;obviously,it was significantly higher in model group than in normal group (t=16.55,P=0.00) and aminocaproic acid tablets group (t=-4.12,P=0.00) and it was significantly lower in GZFLJN group than in model group (t=-15.23,P=0.00) and aminocaproic acid tablets group (t=-12.08,P=0.00). Serum 6-keto-PGF1α level in these four groups were (23.17±1.93),(18.09±0.93),(22.70±1.61),and (20.70±1.41)ng/L,respectively;obviously,it was significantly lower in model group than in normal group (t=-13.98,P=0.00) and aminocaproic acid tablets group (t=5.26,P=0.00) and it was significantly higher in GZFLJN group than in model group (t=11.43,P=0.00) and aminocaproic acid tablets group (t=8.76,P=0.00). Conclusion GZFLJN can regulate the expressions of α-SMA and PCNA of VSMC in the endometrium of IUD rats and the concentrations of TXB2 and 6-keto-PGF1α in the serum.