Phylogeographic analysis reveals extensive genetic variation of native grass Elymus nutans (Poaceae) on the Qinghai-Tibetan plateau.

Introduction: Elymus nutans holds ecological and pastoral significance due to its adaptability and nutritional value, the Qinghai-Tibet Plateau (QTP) is a key hub for its genetic diversity. To conserve and harness its genetic resources in highland ecosystems, a thorough assessment is vital. However, a comprehensive phylogeographic exploration of E. nutans is lacking. The objective of this study was to unravel the genetic diversity, adaptation, and phylogenetics of E. nutans populations. Methods: Encompassing 361 individuals across 35 populations, the species' genetic landscape and dynamic responses to diverse environments were decoded by using four chloroplast DNA (cpDNA) sequences and nine microsatellite markers derived from the transcriptome. Results and discussion: This study unveiled a notable degree of genetic diversity in E. nutans populations at nuclear (I = 0.46, He = 0.32) and plastid DNA levels (Hd = 0.805, π = 0.67). Analysis via AMOVA highlighted genetic variation predominantly within populations. Despite limited isolation by distance (IBD), the Mekong-Salween Divide (MSD) emerged as a significant factor influencing genetic differentiation and conserving diversity. Furthermore, correlations were established between external environmental factors and effective alleles of three EST-SSRs (EN5, EN57 and EN80), potentially linked to glutathione S-transferases T1 or hypothetical proteins, affecting adaptation. This study deepens the understanding of the intricate relationship between genetic diversity, adaptation, and environmental factors within E. nutans populations on the QTP. The findings shed light on the species' evolutionary responses to diverse ecological conditions and contribute to a broader comprehension of plant adaptation mechanisms.

Lifting Wavelet-Assisted EM Joint Estimation and Detection in Cooperative Spectrum Sensing.

Spectrum sensing in Cognitive radio (CR) is a way to improve spectrum utilization by detecting spectral holes to achieve a dynamic allocation of spectrum resources. As it is often difficult to obtain accurate wireless environment information in real-world scenarios, the detection performance is limited. Signal-to-noise ratio (SNR), noise variance, and channel prior occupancy rate are critical parameters in wireless spectrum sensing. However, obtaining these parameter values in advance is challenging in practical scenarios. A lifting wavelet-assisted Expectation-Maximization (EM) joint estimation and detection method is proposed to estimate multiple parameters and achieve full-blind detection, which uses lifting wavelet in noise variance estimation to improve detection probability and convergence speed. Moreover, a stream learning strategy is used in estimating SNR and channel prior occupancy rate to fit the scenario where the SU has mobility. The simulation results demonstrate that the proposed method can achieve comparable detection performance to the semi-blind EM method.

Effects of Climatic Change on Phylogeography and Ecological Niche of the Endemic Herb Elymus breviaristatus on the Qinghai-Tibet Plateau.

Past climatic and topographic variations have created strong biogeographic barriers for alpine species and are key drivers of the distribution of genetic variation and population dynamics of species on the Qinghai-Tibet Plateau (QTP). Therefore, to better conserve and use germplasm resources, it is crucial to understand the distribution and differentiation of genetic variation within species. Elymus breviaristatus, an ecologically important rare grass species with strong resistance, is restricted to a limited area of the QTP. In this study, we investigated the phylogeography of E. breviaristatus using five chloroplast genes and spacer regions in natural populations distributed along the eastern QTP. We identified a total of 25 haplotypes among 216 individuals from 18 E. breviaristatus populations, which were further classified into four haplogroups based on geographical distribution and haplotype network analysis. Notably, we did not observe any signs of population expansion. High genetic diversity was exhibited at both species and population levels, with precipitation being the main limiting factor for population genetic diversity levels. Higher genetic diversity was exhibited by populations located near the Mekong-Salween Divide genetic barrier, suggesting that they may have served as a glacial refuge. The significant pattern of genetic differentiation by environmental isolation highlights the influence of heterogeneous environments on the genetic structure of E. breviaristatus populations. Additionally, the results of ecological niche models indicated that the geographic distribution of E. breviaristatus populations has decreased rapidly since the Last Glacial Maximum but is not threatened by future global warming.

An electronic tongue based on conjugated polymers for the discrimination and quantitative detection of tetracyclines.

A fluorescent sensor array has been developed based on conjugated polymers (CPs) having six different skeletons for the detection of tetracyclines (TCs), which are known as environmental pollutants. CPs were synthesized from confined nanoreactors in a controlled manner. The fluorescent response occurs through the fluorescence resonance energy transfer (FRET) effect. By utilizing linear discriminant analysis (LDA), effective differentiation of TCs was accomplished with a very low detection concentration (66 nM). Moreover, the sensor array exhibited a highly sensitive ability to quantitatively distinguish different concentrations of TCs. Finally, the sensor array's potential for detecting TCs in aqueous solutions has been successfully demonstrated, widening its applications in practical environments. With simple preparation process, a low cost of detection and high sensitivity, the experimental results indicate that the CP-based sensor array is a promising platform for the sensitive and quantitative detection of TCs, and provides a good reference for future scientific research.

An Improved SAMP Algorithm for Sparse Channel Estimation in OFDM System.

Channel estimation of an orthogonal frequency division multiplexing (OFDM) system based on compressed sensing can effectively reduce the pilot overhead and improve the utilization rate of spectrum resources. The traditional SAMP algorithm with a fixed step size for sparse channel estimation has the disadvantages of a low estimation efficiency and limited estimation accuracy. An Improved SAMP (ImpSAMP) algorithm is proposed to estimate the channel state information of the OFDM system. In the proposed ImpSAMP algorithm, the received signal is firstly denoised based on the energy-detection method, which can reduce the interferences on channel estimation. Furthermore, the step size is adjusted dynamically according to the l2 norm of difference between two estimated sparse channel coefficients of adjacent phases to estimate the sparse channel coefficients quickly and accurately. In addition, the double threshold judgment is adopted to enhance the estimation efficiency. The simulation results show that the ImpSAMP algorithm outperforms the traditional SAMP algorithm in estimation efficiency and accuracy.

Contribution of the bacterial community of poorly fermented oat silage to biogas emissions on the Qinghai Tibetan Plateau.

To better utilize poorly fermented oat silage on the Qinghai Tibetan Plateau, 239 samples of this biomass were collected from the plateau temperate zone (PTZ), plateau subboreal zone (PSBZ), and nonplateau climatic zone (NPCZ) in the region and analyzed for microbial community, chemical composition and in vitro gas production. Climatic factors affect the bacterial α-diversity and ß-diversity of poorly fermented oat silage, which led to the NPCZ having the highest relative abundance of Lactiplantibacillus plantarum. Furthermore, the gas production analysis showed that the NPCZ had the highest maximum cumulative gas emissions of methane. Through structural equation modeling analysis, environmental factors (solar radiation) affected methane emissions via the regulation of lactate production by L. plantarum. The enrichment of L. plantarum contributes to lactic acid production and thereby enhances methane emission from poorly fermented oat silage. Notably, there are many lactic acid bacteria detrimental to methane production in the PTZ. This knowledge will be helpful in revealing the mechanisms of environmental factors and microbial relationships influencing the metabolic processes of methane production, thereby providing a reference for the clean utilization of other poorly fermented silage.

Resource Allocation and Data Offloading Strategy for Edge-Computing-Assisted Intelligent Telemedicine System.

Intelligent telemedicine technology has been widely applied due to the quick development of the Internet of Things (IoT). The edge-computing scheme can be regarded as a feasible solution to reduce energy consumption and enhance the computing capabilities for the Wireless Body Area Network (WBAN). For an edge-computing-assisted intelligent telemedicine system, a two-layer network architecture composed of WBAN and Edge-Computing Network (ECN) was considered in this paper. Moreover, the age of information (AoI) was adopted to describe the time cost for the TDMA transmission mechanism in WBAN. According to the theoretical analysis, the strategy for resource allocation and data offloading in edge-computing-assisted intelligent telemedicine systems can be expressed as a system utility function optimizing problem. To maximize the system utility, an incentive mechanism based on contract theory (CT) was considered to motivate edge servers (ESs) to participate in system cooperation. To minimize the system cost, a cooperative game was developed to address the slot allocation in WBAN, while a bilateral matching game was utilized to optimize the data offloading problem in ECN. Simulation results have verified the effectiveness of the strategy proposed in terms of the system utility.

Periodate-Based Perovskite Energetic Materials: A Strategy for High-Energy Primary Explosives.

The requirements for high energy and green primary explosives are more and more stringent because of the rising demand in the application of micro initiation explosive devices. Four new energetic compounds with powerful initiation ability are reported and their performances are experimentally proven as designed, including non-perovskites ([H2 DABCO](H4 IO6 )2 ·2H2 O, named TDPI-0) and perovskitoid energetic materials (PEMs) ([H2 DABCO][M(IO4 )3 ]; DABCO=1,4-Diazabicyclo[2.2.2]octane, M=Na+ , K+ , and NH4 + for TDPI-1, -2, and -4, respectively). The tolerance factor is first introduced to guide the design of perovskitoid energetic materials (PEMs). In conjunction with [H2 DABCO](ClO4 )2 ·H2 O (DAP-0) and [H2 DABCO][M(ClO4 )3 ] (M=Na+ , K+ , and NH4 + for DAP-1, -2, and -4), the physiochemical properties of the two series are investigated between PEMs and non-perovskites (TDPI-0 and DAP-0). The experimental results show that PEMs have great advantages in improving the thermal stability, detonation performance, initiation capability, and regulating sensitivity. The influence of X-site replacement is illustrated by hard-soft-acid-base (HSAB) theory. Especially, TDPIs possess much stronger initiation capability than DAPs, which indicates that periodate salts are in favor of deflagration-to-detonation transition. Therefore, PEMs provide a simple and feasible method for designing advanced high energy materials with adjustable properties.

Joint User Clustering and Graph Coloring Based Pilot Assignment for Cell-Free Massive MIMO Systems.

Pilot contamination due to pilot reuse seriously mitigates the performance of the cell-free massive multiple-input multiple-output (MIMO) systems. In this paper, we propose a joint pilot assignment scheme that employs user clustering and graph coloring (UC-GC) to reduce pilot contamination. The proposed method consists of two steps: firstly, we utilize AP selection to classify all users; secondly, we assign pilots to users with more severe pilot contamination using the graph coloring algorithm and then assign pilots to the remaining users. The numerical simulation results show that the proposed scheme outperforms existing pilot assignment schemes and significantly improves throughout with low complexity.

Yiwei decoction promotes apoptosis of gastric cancer cells through spleen-derived exosomes.

Yiwei decoction (YWD) is a formula of traditional Chinese medicine (TCM) that is clinically effective for the prevention and treatment of gastric cancer recurrence and metastasis. According to the theory of TCM, YWD tonifies the body and strengthens the body's resistance to gastric cancer recurrence and metastasis potentially via the immune regulation of the spleen. The aims of the present study were to investigate whether YWD-treated spleen-derived exosomes in rats inhibit the proliferation of tumor cells, to elucidate the anticancer effects of YWD, and to provide evidence supporting the use of YWD as a new clinical treatment for gastric cancer. Spleen-derived exosomes were obtained by ultracentrifugation and identified by transmission electron microscopy, nanoparticle tracking analysis, and western blot analysis. The location of the exosomes in tumor cells was then determined by immunofluorescence staining. After tumor cells were treated with different concentrations of exosomes, the effect of exosomes on cell proliferation was determined by cell counting kit 8 (CCK8) and colony formation assays. Tumor cell apoptosis was detected by flow cytometry. Particle analysis and western blot analysis identified the material extracted from spleen tissue supernatant as exosomes. Immunofluorescence staining showed that spleen-derived exosomes were taken up by HGC-27 cells, and the CCK8 assay confirmed that the relative tumor inhibition rate of YWD-treated spleen-derived exosomes in the 30 µg/mL reached 70.78% compared to control exosomes in the 30 µg/mL (p < 0.05). Compared to control exosomes in the 30 µg/mL, the colony formation assay indicated that YWD-treated spleen-derived exosomes in the 30 µg/mL colonies have decreased by 99.03% (p < 0.01). Moreover, flow cytometry analysis showed that treatment with YWD-treated exosomes in the 30 µg/mL increased the apoptosis rate to 43.27%, which was significantly higher than that of the control group in the 30 µg/mL (25.91%) (p < 0.05). In conclusion, spleen-derived exosomes from YWD-treated animals inhibit the proliferation of HGC-27 cells via inducing apoptosis, suggesting that spleen-derived exosomes are involved in mediating the antitumor effect of YWD. These results demonstrated a novel exosome-mediated anticancer effect of YWD as a TCM formula, thereby supporting the use of YWD-treated exosomes as a new approach for the clinical treatment of gastric cancer.

Research on the Current Situation and Influencing Factors of College athletics Students' Educational Access Based on Psychological Perspective / Investigación sobre la situación actual y los factores que influyen en el acceso educativo de los estudiantes universitarios de atletismo desde la perspectiva psicológica

Competitive athletes face various factors that affect the athletic as well as the academic of the students. Therefore, this statement describes that how athletes cover their mental stress along aggressive struggle of college life. Sports base students have to deal with their academic performance, financial challenges, and progress efficiency of the sports students. The participation of students in sports is important, as it extends the physical activity expands of our society financially, academically, politically, and psychologically. When it comes to the image of an organization or educational institution, the student-athletes is as a highly visible subgroup of students whose performance and visibility is significantly important for the institution. The social environment, including classrooms, can be seen as a system of stress consisting forces that remain relatively stable over time. The data was collected from 242 athletic students. In this paper, the Perceived Level of Learning Anxiety, Perceived Usefulness of Student’s Learning, and Self-Engagement in Learning Course are the Independent Variables. Whereas, Positive Sports Psychology is a Dependent Variable. The research model had three hypothesis statements. The data was collected through online questionnaire portals. Furthermore, the data was analyzed on SMART PLS 3. The results and findings of the data indicated a positive and significant association between the variables.(AU)

Transcriptomics integrated with metabolomics provides a new strategy for mining key genes in response to low temperature stress in Helictotrichon virescens.

H. virescens is a perennial herbaceous plant with highly tolerant to cold weather, but the key genes that respond to low temperature stress still remain unclear. Hence, RNA-seq was performed using leaves of H. virescens treated at 0 °C and 25 °C for 12 h, 36 h, and 60 h, respectively, and a total of 9416 DEGs were significantly enriched into seven KEGG pathways. The LC-QTRAP platform was performed using leaves of H. virescens leaves at 0 °C and 25 °C for 12 h, 36 h, and 60 h, respectively, and a total of 1075 metabolites were detected, which were divided into 10 categories. Additionally, 18 major metabolites, two key pathways, and six key genes were mined using a multi-omics analytical strategy. The RT-PCR results showed that with the extension of treatment time, the expression levels of key genes in the treatment group gradually increased, and the difference between the treatment group and the control group was extremely significant. Notably, the functional verification results showed that the key genes positively regulated cold tolerance of H. virescens. These results can lay a foundation for the in-depth analysis of the mechanism of response of perennial herbs to low temperature stress.

Efficacy of the neutralizing antibodies after the booster dose on SARS-CoV-2 Omicron variant and a two-year longitudinal antibody study on Wild Type convalescents.

OBJECTIVES: Waning vaccine-induced immunity and emergence of new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants which may lead to immune escape, pose a major threat to the COVID-19 pandemic. Currently, enhanced efficacy of the neutralization antibodies (NAb) produced after the booster dose of vaccinations against the Omicron variant is the main focus of vaccine strategy research. In this study we have analyzed the potency of the NAbs and IgGs produced after the third vaccine dose in patients infected with Omicron variant and wild-type (WT) SARS-CoV-2. METHODS: We enrolled 75 patients with Omicron variant breakthrough infections, and 87 patients with WT infections. We recorded the clinical characteristics and vaccination information of all patients and measured the NAb and anti-S1 (spike protein) + N (nucleocapsid protein) IgG-binding antibodies against SARS-CoV-2 in serum samples of Omicron variant-infected patients at admission, and patients with WT COVID-19 infection from the time of admission and discharge, and one-year to two-years follow-ups. RESULTS: Our results demonstrated higher NAb levels, fewer clinical symptoms, and faster viral shedding in Omicron variant infected patients vaccinated with the booster dose. Hybrid immunity (natural infection plus vaccination) induces higher NAb levels than vaccine-only immunity. NAb and IgG levels decreased significantly at one-year follow-up in WT convalescents with natural infection. The NAb and IgG levels in booster-vaccinated COVID-19 patients were higher than those in two-dose-vaccinated patients. CONCLUSION: Our results suggest that booster vaccinations are required to improve the level of protective NAbs. Moreover, our data provide important evidence for vaccination strategies based on existing vaccines.

Preference-Matched Multitask Assignment for Group Socialization under Mobile Crowdsensing.

Mobile crowdsensing (MCS) has been an emerging sensing paradigm in recent years, which uses a sensing platform for real-time processing to support various services for the Internet of Things (IoT) and promote the development of IoT. As an important component of MCS, how to design task assignment algorithms to cope with the coexistence of multiple concurrent heterogeneous tasks in group-oriented social relationships while satisfying the impact of users' preferences on heterogeneous multitask assignment and solving the preference matching problem under heterogeneous tasks, is one of the most pressing issues. In this paper, a new algorithm, group-oriented adjustable bidding task assignment (GO-ABTA), is considered to solve the group-oriented bilateral preference-matching problem. First, the initial leaders and their collaborative groups in the social network are selected by group-oriented collaboration, and then the preference assignment of task requesters and users is modeled as a stable preference-matching problem. Then, a tunable bidding task assignment process is completed based on preference matching under budget constraints. Finally, the individual reasonableness, stability, and convergence of the proposed algorithm are demonstrated. The effectiveness of the proposed algorithm and its superiority to other algorithms are verified by simulation results.

Characterization and Application of EST-SSR Markers Developed from Transcriptome Sequences in Elymus breviaristatus (Poaceae: Triticeae).

BACKGROUND: Elymus L. is the largest genus in the Triticeae tribe. Most species in this genus are highly stress resistant, with excellent forage value. Elymus breviaristatus, a rare species endemic to the Qinghai-Tibet Plateau (QTP), is declining due to habitat fragmentation. However, genetic data for E. breviaristatus are limited, with expressed sequence tag (EST) markers being particularly rare, hampering genetic studies and protection measures. RESULTS: We obtained 9.06 Gb clean sequences from the transcriptome of E. breviaristatus, generating 171,522 unigenes, which were assembled and functionally annotated against five public databases. We identified 30,668 SSRs in the E. breviaristatus transcriptome, from which 103 EST-SSR primer pairs were randomly selected. Of these, 58 pairs of amplified products of the expected size, and 18 of the amplified products were polymorphic. Model-based Bayesian clustering, the unweighted pair group method with arithmetic average (UPGMA), and principal coordinate analysis (PCoA) of 179 wild E. breviaristatus in 12 populations using these EST-SSRs were generally consistent, grouping the 12 populations into two major clades. Analysis of molecular variance (AMOVA) found 70% of the genetic variation among the 12 populations and 30% within the populations, indicating a high level of genetic differentiation (or low gene exchange) among the 12 populations. The transferability of the 58 successful EST-SSR primers to 22 related hexaploid species was 86.2-98.3%. UPGMA analysis generally grouped species with similar genome types together. CONCLUSIONS: Here, we developed EST-SSR markers from the transcriptome of E. breviaristatus. The transferability of these markers was evaluated, and the genetic structure and diversity of E. breviaristatus were explored. Our results provide a basis for the conservation and management of this endangered species, and the obtained molecular markers represent valuable resources for the exploration of genetic relationships among species in the Elymus genus.

Proteomics Investigation of Diverse Serological Patterns in COVID-19.

Serum antibodies IgM and IgG are elevated during Coronavirus Disease 2019 (COVID-19) to defend against viral attacks. Atypical results such as negative and abnormally high antibody expression were frequently observed whereas the underlying molecular mechanisms are elusive. In our cohort of 144 COVID-19 patients, 3.5% were both IgM and IgG negative, whereas 29.2% remained only IgM negative. The remaining patients exhibited positive IgM and IgG expression, with 9.3% of them exhibiting over 20-fold higher titers of IgM than the others at their plateau. IgG titers in all of them were significantly boosted after vaccination in the second year. To investigate the underlying molecular mechanisms, we classed the patients into four groups with diverse serological patterns and analyzed their 2-year clinical indicators. Additionally, we collected 111 serum samples for TMTpro-based longitudinal proteomic profiling and characterized 1494 proteins in total. We found that the continuously negative IgM and IgG expression during COVID-19 were associated with mild inflammatory reactions and high T cell responses. Low levels of serum IgD, inferior complement 1 activation of complement cascades, and insufficient cellular immune responses might collectively lead to compensatory serological responses, causing overexpression of IgM. Serum CD163 was positively correlated with antibody titers during seroconversion. This study suggests that patients with negative serology still developed cellular immunity for viral defense and that high titers of IgM might not be favorable to COVID-19 recovery.

Do you take off your mask correctly? A survey during COVID-19 pandemic in Ningbo, China.

Guidelines and recommendations from public health authorities related to face masks have been essential for containing the COVID-19 pandemic. A cross-sectional survey was conducted in Ningbo City, China, from April 8 to 12, 2022. We assessed the behavioral differences and correlates of mask usage, primarily mask-removal. We examined public mask-wearing behavior during on-site COVID-19 nucleic acid detection. The survey instrument was developed based on the guidelines issued by the World Health Organization and consisted of demographics, mask-wearing knowledge, and behavior. We analyzed data from 1180 participants; 73.2% demonstrated good mask-wearing knowledge. However, regarding mask-wearing behavior, only 53.7% knew the correct way to remove a mask; 70.3% maintained hand hygiene after touching the outside. Binary logistic regression analyses revealed that health prevention knowledge and free mask distribution were positively associated with two types of mask-wearing behaviors. Most participants used masks during the COVID-19 pandemic; however, mask-removal and hand hygiene were neglected when touching the outside of the mask. More attention must be paid to mask-removal and hand hygiene details. Local health authorities should consider introducing the free distribution of masks.

Ataxia with oculomotor apraxia type 2 caused by a novel homozygous mutation in SETX gene, and literature review.

Objectives: Autosomal recessive inherited ataxia with oculomotor apraxia type 2 (AOA2), caused by SETX gene mutations, is characterized by early-onset, progressive cerebellar ataxia, peripheral neuropathy, oculomotor apraxia and elevated serum α-fetoprotein (AFP). This study aimed to expand and summarize the clinical and genetic characteristics of SETX variants related to AOA2. Methods: The biochemical parameters, electromyogram and radiological findings of the patient were evaluated. Whole-exome sequencing (WES) was performed on the patient using next-generation sequencing (NGS), the variants were confirmed by Sanger sequencing and the pathogenicity of the variants was classified according to the American College of Medical Genetics and Genomics/Association for Molecular Pathology (ACMG/AMP) guidelines. We reviewed 57 studies of AOA2 patients with SETX mutations and collected clinical and genetic information. Results: The patient was a 40-year-old Chinese woman who primarily presented with numbness and weakness of the lower limbs in her teenage years. She had elevated AFP, increased serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH) and decreased anti-Müllerian hormone (AMH) levels. We identified a novel homozygous missense mutation of the SETX gene, c.7118 C>T (p. Thr2373Ile), in the patient via Whole-exome and Sanger sequencing. The variant was located in the DNA/RNA helicase domain and is highly conserved. The protein prediction analysis verified the SETX variant as a damaging alteration and ACMG/AMP guidelines classified it as likely pathogenic. Through a literature review, we identified 229 AOA2 cases with SETX variants, and among the variants, 156 SETX variants were exonic. We found that 107 (46.7%) patients were European, 50 (21.8%) were African and 48 (21.0%) were Asian. Among the Asian patients, five from two families were Mainland Chinese. The main clinical features were cerebellar ataxia (100%), peripheral neuropathy (94.6%), cerebellar atrophy (95.3%) and elevated AFP concentration (92.0%). Most reported SETX mutations in AOA2 patients were missense, frameshift and nonsense mutations. Conclusion: We discovered a novel homozygous variant of the SETX gene as a cause of AOA2 in the current patient and expanded the genotypic spectrum of AOA2. Moreover, the clinical features of AOA2 and genetic findings in SETX were assessed in reported cohorts and are summarized in the present study.

Maximizing Average Throughput of Cooperative Cognitive Radio Networks Based on Energy Harvesting.

Energy harvesting (EH) and cooperative communication techniques have been widely used in cognitive radio networks. However, most studies on throughput in energy-harvesting cooperative cognitive radio networks (EH-CCRNs) are end-to-end, which ignores the overall working state of the network. For the above problems, under the premise of prioritizing the communication quality of short-range users, this paper focuses on the optimization of the EH-CCRN average throughput, with energy and transmission power as constraints. The formulated problem was an unsolved non-deterministic polynomial-time hardness (NP-hard) problem. To make it tractable to solve, a multi-user time-power resource allocation algorithm (M-TPRA) is proposed, which is based on sub-gradient descent and unary linear optimization methods. Simulation results show that the M-TPRA algorithm can improve the average throughput of the network. In addition, the energy consumed by executing the M-TPRA algorithm is analyzed.

Physical condition, psychological status, and posttraumatic stress disorder among recovered COVID-19 subjects: A mediation analysis.

The physical condition of individuals who contracted COVID-19 had a profound influence on mitigating the physical and psychological impact of the disease and the symptoms of posttraumatic stress disorder (PTSD). Little attention has been focused on the influence of physical condition on PTSD among recovered COVID-19 subjects. This study explored the relationship between physical and psychological status and PTSD and the potential mechanisms. Questionnaires were completed by 73 (50.7%, 73/144) COVID-19 recovered subjects who were diagnosed in Taizhou, Zhejiang, China. We conducted a face-to-face survey from January 17 to March 10, 2020. The mediation analysis approach was applied in this research. Our data show that recovered COVID-19 subjects who were in better physical condition exhibited fewer psychological problems [B (95%CI), (-1.65 -3.04, -0.26)] and lower PTSD [B (95%CI), -6.13 (-9.43, -2.83)]. In addition, the worse the psychological status of recovered COVID-19 subjects was, the stronger the PTSD (B [95%CI], 0.58 [0.02, 1.14]). Moreover, psychological status could significantly mediate the impact of physical condition on PTSD (ß1θ2 = -0.87). Together, COVID-19 recovered subjects who have better physical condition could decrease their PTSD, and the worse the physical condition of COVID-19 recovered subjects would increase their psychological problems. Our finding about psychological status could significantly mediate the impact of the physical condition on PTSD might be useful for medical institutions and the government seeking to help with the follow-up rehabilitation training of recovered COVID-19 subjects.