Effect of silk fibroin scaffold loaded with 17-ß estradiol on the proliferation and differentiation of BMSCs.

In this study, different concentrations of 17-ß estradiol silk fibroin (SF)porous scaffolds (SFPS) were prepared using freeze-drying technique, with a hope for optimal concentration and apply it locally to the bone defect area. In this study, the porous scaffold morphology structure was characterized by SEM, FTIR and universal capacity testing machines, and the in vitro cytocompatibility and biological activity of scaffold materials were studied by cell adhesion, viability and proliferation experiments. The results showed that SFPS boasts better physicochemical properties, while 17-ß estradiol SF scaffolds with low concentrations of 10-10 mol/L and 10-12 mol/L had more growth and proliferation of SF scaffolds with higher concentrations, and 10-10 mol/L was the optimal concentration of 17-ß estradiol SFPS, which was more conducive to cell adhesion and proliferation. On the other hand, after osteogenesis induction of BMSCs inoculated on 17-ß estradiol SFPS at different concentrations, it was found that the expression of alkaline phosphatase in BMSCs on different concentrations of 17-ß estradiol porous scaffolds was not large. No conflict of interest exits in the submission of this manuscript.

Research on the Characteristics of Food Impaction with Tight Proximal Contacts Based on Deep Learning.

OBJECTIVE: Based on deep learning, the characteristics of food impaction with tight proximal contacts were studied to guide the subsequent clinical treatment of occlusal adjustment. At the same time, digital model building, software measurement, and statistical correlation analysis were used to explore the cause of tooth impaction and to provide evidence for clinical treatment. METHODS: Volunteers with (n = 250) and without (n = 250) tooth impaction were recruited, respectively, to conduct a questionnaire survey. Meanwhile, models were made and perfused by skilled clinical physicians for these patients, and characteristics such as adjacent line length, adjacent surface area, tongue abduction gap angle, buccal abduction gap angle, and occlusal abduction gap angle were measured. A normality test, differential analysis, correlation analysis of pathological characteristics of the impaction group, principal component analysis (PCA), and binary logistic regression analysis were performed. RESULTS: The adjacent line length, adjacent surface area, tongue abduction gap angle, buccal abduction gap angle, and occlusal abduction gap angle all met normal distribution. There were statistically significant differences in adjacent line length (p < 0.001), adjacent surface area (p < 0.001), and occlusal abduction gap angle (p < 0.001) between the two groups. After dimensionality reduction by PCA on characteristics, adjacent line length, adjacent surface area, buccal abduction gap angle, and occlusal abduction gap angle had a strong correlation with the principal components. Binary logistic regression analysis showed that adjacent line length and adjacent surface area had positive effects on impaction. The buccal abduction gap angle and occlusal abduction gap angle had a significant negative influence on impaction. CONCLUSION: Adjacent line length, adjacent surface area, buccal abduction gap angle, and occlusal abduction gap angle are independent factors influencing food impaction.