ABSTRACT
THE AIM OF THE STUDY: Was to research the muscles response in children during three years of their life to mechanical stimulation with evaluation of the maximum time of its occurrence from 2 to 15 hours of the postmortem period. Assessment of the muscles response to mechanical stress is one of the methods for determining the time of death coming. For the deceased of mature age, there is very little scientific data on this topic and for children and adolescents there is none at all. The results of a study of 244 children corpses aged 1 to 3 years with a precisely established time of death within 2 to 15 hours of the postmortem period are presented. Two 2 age groups have been identified: 1st - from 1 to 2 years, 2nd - from 2 to 3 years. It was determined that the response of muscles to mechanical stress in children depends on age and does not depend on gender. The time of occurrence of the muscle contraction swelling for each age group was established. The results of the study are the first reliable evidence of the postmortem time limit of muscle contraction swelling onset in children of different age groups.
Subject(s)
Neoplasms , Postmortem Changes , Adolescent , Autopsy , Child , Child, Preschool , Humans , Infant , Muscle Contraction , MusclesABSTRACT
Morphofunctional activity of mast cells in renal cancer is studied on specimens from the center of the tumor, peritumorous zone (with an obligatory fragment of the pseudocapsule), and from renal tissues maximally distant from the tumor. The counts and areas of tumor cells were maximum in the peritumorous zone in comparison with intact kidney tissue and central zone of the tumor. Analysis of correlations revealed a direct correlation between the tumor cell count in the peritumorous zone and some clinical morphological parameters of the tumor (anaplasia degree, size, presence of invasion and metastases, and angiogenesis). The count of mast cells in the renal peritumorous zone inversely correlated with 5-year survival: 100% cumulative 5-year survival in patients with mast cell count <6.7±1.6 per field of view.
Subject(s)
Kidney Neoplasms/diagnosis , Mast Cells/pathology , Neovascularization, Pathologic/diagnosis , Biomarkers/analysis , Cell Count , Female , Humans , Kidney/metabolism , Kidney/pathology , Kidney Neoplasms/metabolism , Kidney Neoplasms/mortality , Kidney Neoplasms/pathology , Male , Middle Aged , Neovascularization, Pathologic/metabolism , Neovascularization, Pathologic/mortality , Neovascularization, Pathologic/pathology , Prognosis , Proto-Oncogene Proteins c-kit/analysis , Survival Analysis , Tumor MicroenvironmentABSTRACT
This investigation was aimed at the study of age changes of penis connective tissue structures. Tissue fragments of penis were obtained from 20 cadavers of men at the age of 20-38 years in group I, and from 20 cadavers of men at the age of 41-59 years in group II. The criteria for the exclusion of material from the research were arterial hypertension, diabetes mellitus, atherosclerosis of internal iliac arteries, Peyronie's disease, and anomalies of genital organ development. It was shown that in the cavernous body of penis, aging was associated with the increased amount and thickening of collagen and argyrophilic fibers, decreased content and thinning of elastic fibers, and the reduced amount of smooth muscle cells (SMC). The average area of fibroblast and SMC nucleolus was not different in both groups studied. The average area of endotheliocyte nucleolus was equal to 1.9+/-0.9 microm2 in group II, being lower than that one in group I, in which this index was equal to 2.1+/-0.9 microm2. No differences in the content of type III and IV collagen were found between the study groups. Age-associated decrease in the average area of endothelial cell nucleolus in the cavernous bodies may reflect the reduction of the activity of these cells and may indicate the development of endothelial dysfunction, which is one of the most important steps in the morphogenesis of age-related male erectile dysfunction.
Subject(s)
Aging/pathology , Elastic Tissue/pathology , Erectile Dysfunction/pathology , Penis/pathology , Adult , Aging/metabolism , Cell Nucleus/metabolism , Cell Nucleus/pathology , Collagen/metabolism , Elastic Tissue/metabolism , Endothelial Cells/metabolism , Endothelial Cells/pathology , Erectile Dysfunction/metabolism , Fibroblasts/metabolism , Fibroblasts/pathology , Humans , Male , Middle Aged , Myocytes, Smooth Muscle/metabolism , Myocytes, Smooth Muscle/pathology , Penis/metabolismABSTRACT
The modified method of staining of argyrophilic nucleolar organizer regions (AgNORs) by silver nitrate is presented. This modification eliminates some disadvantages of an original technique. The method accelerates staining and prevents the precipitation of deposits.
