Metals in vegetables from markets in Zaria, Nigeria and risk assessment.

This study, investigated the concentrations of cadmium, lead and arsenic in vegetables grown with irrigation and sold in Sabon gari and Samaru markets in Zaria, Nigeria. Cadmium was absent in amaranthus, pepper and tomatoes purchased from Samaru market. Nevertheless, amaranthus and lettuce had higher concentrations of these toxic metals than pepper. Total arsenic concentrations in the investigated vegetables were higher than the maximum levels set by the World Health Organization. Total daily intake of the metals was higher than the maximum levels for consuming vegetables from these markets. Therefore, individuals who consume these foods may be at risk. These results indicate the possibility of toxic metal contamination in vegetables purchased from Zaria markets.

Identification of novel laccase from cyanobacterium Microcystis flos-aquae and enhanced azo dye bioremediation potential.

Textile industries discharge up to 280,000 tons of dye waste annually, resulting in global pollution and health risks. In Nigeria and other African countries, persistent dyes threaten aquatic life and human health. This study introduces a cost-effective, enzyme-mediated bioremediation alternative using a novel laccase from the cyanobacteriumMicrocystis flos-aquae. This purified enzyme yielded 0.55 % (w/w)with significant activity at 40 °C and pH 4.00. Kinetic studies showed the dependence of M. flos-aquae laccase on Cu2+and its inhibition by EDTA and Fe2+. The efficacy of the enzyme was demonstrated through rapid decolorization of the azo dye Cibacron Brilliant Blue over a wide temperature and pH range. As this enzyme effectively decolorizes dyes across a broad temperature and pH range, it offers a promising solution for bioremediation of textile effluents.

Unveiling the link between Raphidiopsis raciborskii blooms and saxitoxin levels: Evaluating water quality in tropical reservoirs, Brazil.

The proliferation of Raphidiopsis raciborskii blooms has sparked concerns regarding potential human exposure to heightened saxitoxins (STXs) levels. Thus, comprehending how environmental elements drive the proliferation of this STXs-producing species can aid in predicting human exposure risks. This study aimed to explore the link between cyanobacteria R. raciborskii, STXs cyanotoxins, and environmental factors in 37 public supply reservoirs in the tropical region and assess potential health hazards these toxins pose in the reservoir waters. A Structural Equation Model was used to assess the impact of environmental factors (water volume and physical and chemical variables) on R. raciborskii biomass and STXs levels. Furthermore, the potential risk of STXs exposure from consuming untreated reservoir water was evaluated. Lastly, the cumulative distribution function (CDF) of STXs across the reservoirs was computed. Our findings revealed a correlation between R. raciborskii biomass and STXs concentrations. Total phosphorus emerged as a critical environmental factor positively influencing species biomass and indirectly affecting STXs levels. pH significantly influenced STXs concentrations, indicating different factors influencing R. raciborskii biomass and STXs. Significantly, for the first time, the risk of STXs exposure was gauged using the risk quotient (HQ) for untreated water consumption from public supply reservoirs in Brazil's semi-arid region. Although the exposure risks were generally low to moderate, the CDF underscored the risk of chronic exposure due to low toxin concentrations in over 90% of samples. These outcomes emphasize the potential expansion of R. raciborskii in tropical settings due to increased phosphorus, amplifying waterborne STXs levels and associated intoxication risks. Thus, this study reinforces the importance of nutrient control, particularly phosphorus regulation, as a mitigation strategy against R. raciborskii blooms and reducing STXs intoxication hazards.

Nitrogen forms and concentration influence the impact of titanium dioxide nanoparticles on the biomass and antioxidant enzyme activities of Microcystis aeruginosa.

Nanoparticles (NPs) are becoming more widely produced, used, and released into the aquatic environment. In aquatic ecosystems, these NPs affect different populations of photosynthesizing organisms, such as cyanobacteria. This study aimed to evaluate the effects of titanium dioxide (TiO2) NPs (48 mg l-1) combined with low (0.04 mM) and high (9 mM) concentrations of urea and nitrate on Microcystis aeruginosa. Microcystins (MCs) production and release were monitored in the cyanobacterium. The results showed that high urea concentration (9 mM) combined with TiO2 NPs inhibited growth, pigment, and malondialdehyde (MDA) content by 82%, 63%, and 47%, respectively. The treatment also increased the reactive oxygen species (ROS) and glutathione S-transferase (GST) activity by 40.7% and 67.7%, respectively. Similarly, low nitrate (0.04 mM) combined with TiO2 NPs inhibited growth by 40.3% and GST activity by 36.3% but stimulated pigment production and ROS concentration in M. aeruginosa. These responses suggest that high urea combined with TiO2.NPs and high nitrate combined with TiO2 NPs induced oxidative stress in cyanobacteria. The peroxidase (POD) activity of M. aeruginosa decreased by 17.7% with increasing urea concentrations. Our findings suggest that TiO2 NPs combined with changing nutrient (urea and nitrate) concentrations may adversely affect cyanobacterial development and antioxidant defense enzymes.

Risk assessment of the antibiotic amoxicillin on non-toxin-producing strains and toxin-producing strains of Microcystis.

Amoxicillin (AMX) is a common antibiotic used to treat a variety of infectious illnesses in humans and animals, including otitis media, tonsillitis, tonsillopharyngitis, laryngitis, and pharyngitis. The drug ends up in the aquatic ecosystems through animal and human excretion and industrial effluents. The ecological consequences of broad-spectrum antibiotics on non-target species like cyanobacteria are causing considerable concern. The danger of amoxicillin to non-toxin-producing and toxin-producing strains of cyanobacteria is poorly understood. The objective of this study was to analyze the risk (RQ) and physiological effects of AMX on Microcystis aeruginosa EAWAG 198 (non-toxin producing = NTP), Microcystis aeruginosa LE3 (toxin-producing = TP), and Microcystis flos aquae UTEX-LB 2677 (toxin-producing = TP). Our study showed differences in the RQ of the drug to the tested organisms - demonstrating < Microcystis flos aquae UTEX-LB 2677 > Microcystis aeruginosa LE3 > Microcystis aeruginosa EAWAG 198. The calculated EC50 values show that AMX was more toxic to the toxin-producing strains than the non-toxin-producing strains. Amoxicillin led to significant (p < 0.05) growth inhibition and chlorophyll-a content of the exposed cultures. The observed increase in the concentration of intracellular hydrogen peroxide (H2O2) of the exposed cultures at 96 h was significant (p < 0.05), demonstrating that the expressed oxidative stress patterns observed during the study were due to AMX. The current study shows significant variation (p < 0.05) in melondialdehyde (MDA) content and the antioxidant enzymes - glutathione-S-transferase (GST) and peroxidase (POD).

Immobilization, oxidative stress and antioxidant response of Daphnia magna to Amoxicillin and Ciprofloxacin.

Amoxicillin (AMX) and ciprofloxacin (CPX) are broad-spectrum antibiotics with wide application in agriculture and human and veterinary medicine. The drugs end up in the environment where their impact on zooplankton remains scantily understood. This study investigated the immobilization, risk assessment (RQ), antioxidant response, and biochemical changes of Daphnia magna post-exposure to AMX and CPX. Sixty-percent immobilization of Daphnia occurred at 200 µg L-1 AMX and CPX, while EC50 values were 2391.6 µg L-1 and 273.4 µg L-1, respectively. RQs were 113.3 and 11,481.5, while Toxic units were 41.6 and 364.9 for AMX and CPX, respectively. Both antibiotics caused a significant rise in intracellular hydrogen peroxide 48 h post-exposure, indicating oxidative stress. Lipid peroxidation and antioxidant enzyme activity were considerably altered during the research. Thus, environmentally relevant concentrations of AMX and CPX pose an adverse risk that could change the population dynamics of Daphnia magna.

The growth, biochemical composition, and antioxidant response of Microcystis and Chlorella are influenced by Ibuprofen.

Non-steroidal anti-inflammatory drugs like ibuprofen (IBU) are extensively used, causing substantial amounts to end up in aquatic ecosystems. Unfortunately, little research has been done on how these medications influence the physiology of phytoplankton. This study aimed to investigate the toxicological and physiological effects of IBU on the cyanobacteria Microcystis aeruginosa LE3 and Microcystis aeruginosa EAWAG 198, and the chlorophyte Chlorella sorokiniana. Exponential growth phase cultures were exposed to IBU at 10 to 10,000 µg/L for 96 h. The medium effect concentrations revealed varied sensitivity to IBU in the order Chlorella sorokiniana > Microcystis aeruginosa LE3 > Microcystis aeruginosa EAWAG 198. The drug caused a significant difference from control in cell density and chlorophyll-a of the three strains, except for chlorophyll-a in M. aeruginosa EAWAG 198 cultures where a significant difference occurred at 100 µg/L. The cell density of M. aeruginosa LE3 cultures exposed to 10 µg/L IBU increased 24 h post-exposure. Increasing concentrations of IBU induced higher total microcystins content of the Microcystis aeruginosa. Intracellular hydrogen peroxide content, peroxidase, and glutathione S-transferase activities, and lipid peroxidation increased as a function of IBU exposure. Total lipid, carbohydrate, and protein content of Chlorella sorokiniana were stimulated following IBU exposure. We conclude that the increasing presence of IBU in aquatic ecosystems could significantly alter the population dynamics of the investigated and other phytoplankton species.

Genetic Diversity of Microcystin Producers (Cyanobacteria) and Microcystin Congeners in Aquatic Resources across Africa: A Review Paper.

Microcystins are produced by multifaceted organisms called cyanobacteria, which are integral to Africa's freshwater environments. The excessive proliferation of cyanobacteria caused by rising temperature and eutrophication leads to the production and release of copious amounts of microcystins, requiring critical management and control approaches to prevent the adverse environmental and public health problems associated with these bioactive metabolites. Despite hypotheses reported to explain the phylogeography and mechanisms responsible for cyanobacterial blooms in aquatic water bodies, many aspects are scarcely understood in Africa due to the paucity of investigations and lack of uniformity of experimental methods. Due to a lack of information and large-scale studies, cyanobacteria occurrence and genetic diversity are seldom reported in African aquatic ecosystems. This review covers the diversity and geographical distribution of potential microcystin-producing and non-microcystin-producing cyanobacterial taxa in Africa. Molecular analyses using housekeeping genes (e.g., 16S rRNA, ITS, rpoC1, etc.) revealed significant sequence divergence across several cyanobacterial strains from East, North, West, and South Africa, but the lack of uniformity in molecular markers employed made continent-wise phylogenetic comparisons impossible. Planktothrix agardhii, Microcystis aeruginosa, and Cylindrospermopsis raciborskii (presently known as Raphidiopsis raciborskii) were the most commonly reported genera. Potential microcystin (MCs)-producing cyanobacteria were detected using mcy genes, and several microcystin congeners were recorded. Studying cyanobacteria species from the African continent is urgent to effectively safeguard public and environmental health because more than 80% of the continent has no data on these important microorganisms and their bioactive secondary metabolites.

Opposite Sides of Pantoea agglomerans and Its Associated Commercial Outlook.

Multifaceted microorganisms such as the bacterium Pantoea colonize a wide range of habitats and can exhibit both beneficial and harmful behaviors, which provide new insights into microbial ecology. In the agricultural context, several strains of Pantoea spp. can promote plant growth through direct or indirect mechanisms. Members of this genus contribute to plant growth mainly by increasing the supply of nitrogen, solubilizing ammonia and inorganic phosphate, and producing phytohormones (e.g., auxins). Several other studies have shown the potential of strains of Pantoea spp. to induce systemic resistance and protection against pests and pathogenic microorganisms in cultivated plants. Strains of the species Pantoea agglomerans deserve attention as a pest and phytopathogen control agent. Several of them also possess a biotechnological potential for therapeutic purposes (e.g., immunomodulators) and are implicated in human infections. Thus, the differentiation between the harmful and beneficial strains of P. agglomerans is mandatory to apply this bacterium safely as a biofertilizer or biocontroller. This review specifically evaluates the potential of the strain-associated features of P. agglomerans for bioprospecting and agricultural applications through its biological versatility as well as clarifying its potential animal and human health risks from a genomic point of view.

Assessment of microcystins in surface water and irrigated vegetables in Kwaru stream, Hayin Danmani, Kaduna-Nigeria.

Microcystins (MCs) are hepatotoxic secondary metabolites produced by several genera of cyanobacteria. Human exposure routes include drinking contaminated water and consuming contaminated fish, vegetables, crops, and even food supplements that contain cyanotoxin. This study investigated the presence of MCs in a stream with a long history of use as a source of water for irrigation farming. To establish the risk of a lack of monitoring programs, we studied MCs contamination of irrigated vegetables. Toxin levels in the water were generally <0.05 µg L-1 in all the investigated stations. Total microcystin concentrations in spinach (0.306 µg Kg-1 upstream and 0.217 µg Kg-1 downstream), lettuce (0.085 µg Kg-1 upstream and 0.462 µg Kg-1 downstream), carrot (0.050 µg Kg-1 downstream and 0.116 µg Kg-1 downstream), cabbage (0.014 µg Kg-1 upstream and 0.031 µg Kg-1 downstream), tomatoes (0.233 µg Kg-1 upstream), and bitter leaf (0.460 µg Kg-1 upstream and 0.050 µg Kg-1 downstream) collected in March were higher than the levels detected in samples of the same vegetables collected in April. These results highlight Nigeria's severe public health problem, especially in regions with long dry season spells. The level of MCs contamination of irrigated vegetables implies a potential for chronic exposure and associated health challenges.

Effect of ultraviolet radiation (type B) and titanium dioxide nanoparticles on the interspecific interaction between Microcystis flos-aquae and Pseudokirchneriella subcapitata.

Anthropogenic activities have led to the depletion of the ultraviolet radiation screening ozone layer, exposing aquatic biota to its harmful effects. Also, the rising applications of nanotechnology are resulting in the release and contamination of aquatic ecosystems with engineered nanometals like titanium dioxide nanoparticles (nTiO2). The rise in ultraviolet radiation interacts with nanometals, increasing their bioactivities to susceptible aquatic organisms such as algae and cyanobacteria. The effect of ultraviolet radiation B (UVB) and nTiO2 on Microcystis flos-aquae and Pseudokirchneriella subcapitata during inter-specific interaction was investigated. The specific growth rate (d-1) of M. flos-aquae exposed to nTiO2 increased significantly under monoculture conditions but was suppressed during co-culture with P. subcapitata. Contrarily, UVB stimulated the growth of the cyanobacterium regardless of the presence or absence of the green microalgae. However, there was a general decline in the growth of P. subcapitata following cultivation with M. flos-aquae and exposure to UVB and nTiO2. The chlorophyll-a and total chlorophyll content of the monocultures of M. flos-aquae exposed to nTiO2 increased while other co-culture treatments significantly decreased these parameters. The experimental treatments, UVB, nTiO2, and UVB + nTiO2 had differential effects on the pigment content of P. subcapitata. The total protein content, intracellular H2O2, peroxidase (POD), and glutathione S-transferase (GST) activity of both M. flos-aquae and P. subcapitata increased at varying degrees as a function of the treatment condition. Microcystin content was highest in co-cultures exposed to UVB. The results of this study suggest that increasing levels of nTiO2 and UVB significantly alter the growth and cellular metabolic activity of M. flos-aquae and P. subcapitata, but the cyanobacterium will probably be favored by increasing UVB levels and its interaction with nanometals like nTiO2 in aquatic ecosystems.

Susceptibility of phytoplankton to the increasing presence of active pharmaceutical ingredients (APIs) in the aquatic environment: A review.

Human and veterinary pharmaceuticals either in the form of un-metabolized, incompletely metabolized, and metabolized drugs are increasingly present in aquatic ecosystems. These active pharmaceutical ingredients from pharmaceutical industries, hospitals, agricultural, and domestic discharges find their way into water systems - where they adversely affect non-target organisms like phytoplankton. Different aspects of phytoplankton life; ranging from growth, reproduction, morphology, physiology, biochemical composition, oxidative response, proteomics, and transcriptomics are altered by pharmaceuticals. This review discusses the currently available information on the susceptibility of phytoplankton to the ever-increasing presence of pharmaceutical products in the aquatic environment by focusing on the effect of APIs on the physiology, metabolome, and proteome profiles of phytoplankton. We also highlight gaps in literature concerning the salient underlining biochemical interactions between phytoplankton communities and pharmaceuticals that require an in-depth investigation. This is all in a bid to understand the imminent dangers of the contamination of water bodies with pharmaceutical products and how this process unfavorably affects aquatic food webs.

Effects of the antimalarial lumefantrine on Lemna minor, Raphidocelis subcapitata and Chlorella vulgaris.

Lumefantrine is used to treat uncomplicated malaria caused by pure or mixed Plasmodium falciparum infections and as a prophylactic against recrudescence following artemether therapy. However, the pharmaceutical is released into the aquatic environment from industrial effluents, hospital discharges, and human excretion. This study assessed the effects of lumefantrine on the growth and physiological responses of the microalgae Chlorella vulgaris and Raphidocelis subcapitata (formerly known as Selenastrum capricornutum and Pseudokirchneriella subcapitata) and the aquatic macrophyte Lemna minor. The microalgae and macrophyte were exposed to 200-10000 µg l-1 and 16-10000 µg l-1 lumefantrine, respectively. Lumefantrine had a variable effect on the growth of the aquatic plants investigated. There was a decline in the growth of R. subcapitata and L. minor post-exposure to the drug. Contrarily, there was stimulation in the growth of Chlorella vulgaris. All experimental plants had a significant increase in lipid peroxidation, which was accompanied by an increase in malondialdehyde content. Peroxidase activity of L. minor increased only at low lumefantrine concentrations, while the opposite occurred at higher levels of the drug. Incubation in lumefantrine contaminated medium significantly up-regulated the activity of R. subcapitata cultures. Glutathione S-transferase of L. minor exposed to lumefantrine treatments had substantially higher activities than the controls. Our findings suggest lumefantrine could have adverse but variable effects on the growth and physiology of the studied aquatic plants.

Farmed tilapia as an exposure route to microcystins in Zaria-Nigeria: A seasonal investigation.

Several studies have reported the contamination of farmed fish by microcystins, however, alternations in levels of contamination resulting from seasonal changes are infrequently described. This investigation is focused on the seasonal accumulation of microcystins in farmed Nile Tilapia muscle tissue across three farms located in Zaria, Nigeria, as a means of assessing the health risks associated with the consumption of contaminated fish. Total microcystins and cyanobacteria content, respectively, in muscle tissue and gut of tilapia varied, seasonally in the farms. Microcystin levels were higher in fish tissues analyzed in the dry season than the rainy season at Nagoyi and Danlami ponds. Correlating with the levels of microcystins found in fish tissues, the highest dissolved microcystins levels in all the fish farms occurred in the dry season, where the Bal and Kol fish farm had the highest concentration (0.265 ± 0.038 µgL-1). Gut analysis of fish obtained from the ponds, revealed a predominance of Microcystis spp. among other cyanobacteria. Estimation of total daily intake of consumed contaminated Nile tilapia muscles reveal values exceeding WHO recommended (0.04 µg kg-1 body weight) total daily intake of MC-LR. Consumption of tilapia from Danlami pond presented the greatest risk with a value of 0.093 µg kg-1 total daily intake. Results of the present study necessitate the implementation of legislation and monitoring programs for microcystins and other cyanobacteria contaminants of fish obtained from farms and other sources in Zaria and indeed several other African countries.

The combined effect of clethodim (herbicide) and nitrogen variation on allelopathic interactions between Microcystis aeruginosa and Raphidiopsis raciborskii.

The large-scale use of herbicides deteriorates water quality and threatens aquatic biodiversity. Unfortunately, there are few studies on the ecological effects of herbicides on toxin-producing strains of cyanobacteria under changing nutrient conditions. The objective of the present study was to investigate the effects of the herbicide clethodim and nitrogen variation on the allelopathic interactions and toxin production of Microcystis aeruginosa BCCUSP232 and Raphidiopsis raciborskii (formerly known as Cylindrospermopsis raciborskii) ITEPA1. M. aeruginosa had increased cell density when exposed to the clethodim (H +) (23.55 mg/L), whereas the highest cell density of R. raciborskii was observed in the treatment with clethodim plus limited nitrogen. Also, the cell-free exudate of R. raciborskii significantly stimulated the growth of M. aeruginosa on day 3 of the experiment. The concentration of chlorophyll-a in M. aeruginosa cultures generally increased in all the treatments, while in R. raciborskii cultures, the opposite occurred. Total microcystins (MCs) content of M. aeruginosa in the mixed cultures was 68% higher in nitrogen-enriched conditions than the control. A similar increase in MC content occurred in M. aeruginosa unialgal culture treated with R. raciborskii exudate. Total saxitoxin concentration was 81% higher in mixed cultures of R. raciborskii simultaneously exposed to high nitrogen and clethodim. Similarly, unialgal cultures of R. raciborskii exposed to either high nitrogen or clethodim had higher saxitoxins concentrations than the control. The intracellular H2O2 content of M. aeruginosa cultures decreased, whereas, in R. raciborskii cultures, it increased during exposure to high nitrogen and clethodim. Only R. raciborskii had a significant variation in peroxidase activity. The activities of glutathione S-transferase of both strains were higher in the presence of clethodim. These results revealed that nitrogen enrichment and the presence of clethodim might lead to the excessive proliferation of M. aeruginosa and R. raciborskii and increased production of cyanotoxins in aquatic environments.

The presence of microcystins in the coastal waters of Nigeria, from the Bights of Bonny and Benin, Gulf of Guinea.

Microcystins (MCs) are the most studied toxins of cyanobacteria in freshwater bodies worldwide. However, they are poorly documented in coastal waters in several parts of the world. In this study, we investigated the composition of cyanobacteria and the presence of microcystins (MCs) in several coastal aquatic ecosystems of Nigeria. Direct morphological analysis revealed that members of the genus Oscillatoria were dominant with five species, followed by Trichodesmium with two species in Nigerian coastal waters. Oso Ibanilo had the highest cyanobacterial biomass (998 × 103 cells/L), followed by Rivers Ocean (156 × 103 cells/L). Except for the Cross River Ocean, cyanobacteria were present in all the investigated aquatic ecosystems. Ten (10) out of twenty water bodies examined had detectable levels of MCs. Furthermore, genomic DNA analysis for the mcyE gene of microcystin synthetase (mcy) cluster showed identities higher than 86% (query coverage > 96%) with toxic strains of cyanobacteria in all the samples analyzed. Also, the sequences of samples matched those of uncultured cyanobacteria from recreational lakes in Southern Germany. Our findings indicate that the presence of toxic cyanobacteria in coastal waters of Nigeria is of public and environmental health concern.

Clethodim (herbicide) alters the growth and toxins content of Microcystis aeruginosa and Raphidiopsis raciborskii.

Increased agricultural intensification goes with the widespread use of herbicides that adversely affect aquatic biodiversity. The effects of herbicides on toxin-producing cyanobacteria have been poorly studied. The present study aimed to investigate the toxicological and physiological effects of the herbicide clethodim on Raphidiopsis raciborskii (a.k.a. Cylindrospermopsis raciborskii) ITEPA1 and Microcystis aeruginosa BCCUSP232. On day four of the experiment, the exposure to 25 mg/L clethodim resulted in the highest cell density of R. raciborskii. Similarly, exposure to the 1, 5, 20, and 50 mg/L clethodim treatments resulted in the highest cell densities of M. aeruginosa on day 4 of the experiment. Medium effect concentrations (EC50) after 96 h of exposure of both strains to clethodim were 192.98 mg/L and 168.73 mg/L for R. raciborskii and M. aeruginosa, respectively. The presence of clethodim significantly increased the total microcystin content of M. aeruginosa compared to the control cultures. At 400 mg/L, total saxitoxins content of R. raciborskii was 27% higher than that of the control cultures on day 4. In contrast, cultures exposed to 100 mg/L clethodim had the lowest saxitoxins levels per cell quota. There was an increase in the levels of intracellular hydrogen peroxide in both species during exposure to clethodim, which was followed by significant changes (p < 0.05) in the activity of antioxidant enzymes such as peroxidase and superoxide dismutase. These results revealed that the presence of low levels of clethodim in the aquatic environment might lead to the excessive proliferation of cyanobacteria and alteration of their cyanotoxins content.

Assessment of microcystin contamination of Amaranthus hybridus, Brassica oleracea, and Lactuca sativa sold in markets: a case study of Zaria, Nigeria.

Microcystins (MCs) are toxic secondary metabolites produced by several cyanobacteria genera that have been implicated in human cancer cases and deaths. Human exposure routes include direct contact with contaminated water and the consumption of contaminated food. The present study investigated the presence of MCs in three commonly consumed vegetables at the point of sale in market places as a means of assessing the direct human health risk of buying vegetables. Overall, 53% of the vegetables obtained from different markets had levels of MCs that were higher than 1.00 µg/g. Amaranthus hybridus L. (smooth amaranth) had the highest MC concentration (4.79 µg/g) in samples obtained from Sabon Gari Market, while Lactuca sativa L. (garden lettuce) had the lowest concentration (0.17 µg/g) in samples obtained from Dan-Magaji Market. The highest total daily intake (TDI) of MCs by an adult weighing 60 kg was 3.19 µg/kg for A. hybridus, 1.41 µg/kg for Brassica oleracea L. (cabbage), and 2.94 µg/kg for L. sativa. The highest TDI of MCs for a child weighing 25 kg was highest in A. hybridus (1.91 µg/kg), followed by L. sativa (1.77 µg/kg). These results revealed that the consumption of vegetables sold in markets in Zaria, Nigeria, during the dry season represents a major exposure route to MCs. There is, therefore, an urgent need to develop policies and monitoring strategies to tackle this problem in developing countries.

Effect of flavonoids isolated from Tridax procumbens on the growth and toxin production of Microcystis aeruginos.

The excessive proliferation of toxin producing cyanobacteria constitutes a significant health risk to the environment and humans. This is due to the contamination of potable water and accumulation of cyanotoxins in plant and animal tissues. As a means of controlling bloom forming cyanobacteria, secondary metabolites with pro-oxidative activities from plants are used to treat water bodies contaminated with cyanobacterial blooms and their associated toxins. The objective of the present study was to evaluate the mechanism of action of extract, fractions and isolated flavonoids of Tridax procumbens L. on Microcystis aeruginosa (Kützing) Kützing. by monitoring changes in growth, oxidative stress, antioxidant response, and cyanatoxin microcystins (MCs) production. The extract, fraction 3 and the isolated flavonoids significantly reduced the cell density of the cyanobacterium. Furthermore, the extract and fraction 3 increased the production of reactive oxygen species, induced lipid peroxidation, and altered antioxidant enzyme activities of M. aeruginosa. The total MCs content of the cyanobacterium was negatively affected by the presence of the extract, fractions and isolated flavonoids. The present study show that T. procumbens has secondary metabolites that are capable of interfering with the physiology and microcystins production of M. aeruginosa. These characteristics are promising for the control of this noxious cyanobacterium in aquatic ecosystems.

Cyanobacterial biodiversity of semiarid public drinking water supply reservoirs assessed via next-generation DNA sequencing technology.

Next-generation DNA sequencing technology was applied to generate molecular data from semiarid reservoirs during well-defined seasons. Target sequences of 16S-23S rRNA ITS and cpcBA-IGS were used to reveal the taxonomic groups of cyanobacteria present in the samples, and genes coding for cyanotoxins such as microcystins (mcyE), saxitoxins (sxtA), and cylindrospermopsins (cyrJ) were investigated. The presence of saxitoxins in the environmental samples was evaluated using ELISA kit. Taxonomic analyses of high-throughput DNA sequencing data showed the dominance of the genus Microcystis in Mundaú reservoir. Furthermore, it was the most abundant genus in the dry season in Ingazeira reservoir. In the rainy season, 16S-23S rRNA ITS analysis revealed that Cylindrospermopsis raciborskii comprised 46.8% of the cyanobacterial community in Ingazeira reservoir, while the cpcBAIGS region revealed that C. raciborskii (31.8%) was the most abundant taxon followed by Sphaerospermopsis aphanizomenoides (17.3%) and Planktothrix zahidii (16.6%). Despite the presence of other potential toxin-producing genera, the detected sxtA gene belonged to C. raciborskii, while the mcyE gene belonged to Microcystis in both reservoirs. The detected mcyE gene had good correlation with MC content, while the amplification of the sxtA gene was related to the presence of STX. The cyrJ gene was not detected in these samples. Using DNA analyses, our results showed that the cyanobacterial composition of Mundaú reservoir was similar in successive dry seasons, and it varied between seasons in Ingazeira reservoir. In addition, our data suggest that some biases of analysis influenced the cyanobacterial communities seen in the NGS output of Ingazeira reservoir.