Long Noncoding RNA LOC550643 Acts as an Oncogene in the Growth Regulation of Colorectal Cancer Cells.

Long noncoding RNAs play a key role in the progression of colorectal cancer (CRC). However, the role and mechanism of LOC550643 in CRC cell growth and metastasis remain largely unknown. In this study, we assessed the clinical impacts of LOC550643 on CRC through the analysis of The Cancer Genome Atlas database, which revealed the significant upregulation of LOC550643 in CRC. Moreover, the high expression of LOC550643 was associated with poor survival in patients with CRC (p = 0.001). Multivariate Cox regression analysis indicated that LOC550643 overexpression was an independent prognostic factor for shorter overall survival in patients with CRC (adjusted hazard ratio, 1.90; 95% confidence interval, 1.21-3.00; p = 0.006). A biological function analysis revealed that LOC550643 knockdown reduced colon cancer cell growth by hindering cell cycle progression. In addition, LOC550643 knockdown significantly induced cell apoptosis through the inhibition of signaling activity in phosphoinositide 3-kinases. Moreover, LOC550643 knockdown contributed to the inhibition of migration and invasion ability in colon cancer cells. Furthermore, miR-29b-2-5p interacted with the LOC550643 sequence. Ectopic miR-29b-2-5p significantly suppressed colon cancer cell growth and motility and induced cell apoptosis. Our findings suggest that, LOC550643-miR-29b-2-5p axis was determined to participate in the growth and metastasis of colon cancer cells; this could serve as a useful molecular biomarker for cancer diagnosis and as a potential therapeutic target for CRC.

An aqueous-based process to bioactivate poly(ε-caprolactone)/mesoporous bioglass composite surfaces by prebiotic chemistry-inspired polymer coatings for biomedical applications.

Despite the wide use of aliphatic polyesters, such as poly(L-lactic acid) (PLLA) and poly(ε-caprolactone) (PCL), for many biomedical applications, these materials are limited due to their hydrophobic properties and lack of functional groups to bond with ligands to enhance the cell reorganization. Recently, a composite consisting of bioglass and PCL was demonstrated to enhance the mechanical strength and to improve the degradation rate. Although numerous approaches have been developed to improve the wettability of aliphatic polyesters to create a favorable interface with cells, only few surface modification methods can be independently applied to surfaces with different material. In this work, mesoporous bioglass (MBG) nanoparticles embedded in PCL films were modified by the polymerization of aminomalonitrile (AMN) with 3,4,5-trihydroxybenzaldehyde (THBA). The copolymer layer was further utilized as a mediator to conjugate chitosan and evaluate the antibacterial efficacy. Our results show that the hydrophilicity of the composite membranes significantly improved after treatment. In addition, after immersion in simulated body fluid (SBF) for 14 days, hydroxyapatite formation was only observed on the treated membranes. This result demonstrates that the surface treatment did not alter the MBG bioactivity. Moreover, the cell culture results reveal that the extension level of cells and expression of alkaline phosphatase activity (ALP) of osteoblast-like (MG63) cells were higher on treated composite films compared to untreated ones. The results imply that the treatment procedure can be simultaneously and homogeneously applied to the organic/inorganic composites. In addition, Staphylococcus aureus adhesion on AMN-co-THBA and chitosan/ AMN-co-THBA was significantly lower than untreated PCL. Moreover, the percentage of dead bacteria was highest on the chitosan/ AMN-co-THBA membranes. These results indicate that the AMN-co-THBA modification can be used in composite materials and complex constructs, and it provides a potential method to create versatile surface properties for biomedical applications.