Subject(s)
Societies, Veterinary , Veterinary Medicine , Humans , United Kingdom , Veterinary Medicine/standards , SpecializationABSTRACT
Anesthesia is frequently required to provide appropriate medical care to captive great apes. Anesthetic safety can be optimized through placement of an arterial catheter, which allows direct measurement of arterial blood pressure and easy sampling of arterial blood for blood-gas analysis. Arterial catheterization in great apes can be achieved through palpation-guided or ultrasound-guided placement with or without a modified Seldinger technique. Potential sites for arterial catheterization include the anterior tibial artery, caudal tibial and posterior saphenous arteries, dorsal pedal artery, femoral artery, radial artery, and brachial artery. Arterial catheterization is recommended for lengthy great ape anesthetic procedures or those involving invasive procedures.
Subject(s)
Anesthetics , Catheterization, Peripheral , Hominidae , Animals , Catheterization, Peripheral/methods , Catheterization, Peripheral/veterinary , Radial Artery , Femoral ArteryABSTRACT
The current manuscript highlights the aspects of an animal welfare program for a modern zoological facility. The program should be proactive to identify areas for continuous improvement as well as reactive to address any identified animal welfare concerns. The program should go beyond the five freedoms and utilize one of the more modern frameworks as a foundation for the program. The program should have an animal welfare committee where staff can submit animal welfare concerns without fear of retaliation. Ongoing monitoring of all individual animals should utilize both positive and negative indicators of welfare. Staff should be trained on the most current science and be able to understand key concepts about animal welfare. Facilities should also utilize new scientific findings to continuously improve animal care practices. Modern zoological institutions, including both zoos and aquariums, have an ethical responsibility to provide high levels of animal welfare for the animals under their professional care. Simply meeting minimum standards developed decades ago is not adequate, as animals should have the opportunity to thrive.
ABSTRACT
Four clinically healthy red wolves (Canis rufus) developed hyperkalemia during routine anesthetic procedures. All cases were anesthetized using a combination of dexmedetomidine (10-24 mcg/kg), ketamine (2-3 mg/kg), and either midazolam (0.25-0.5 mg/kg) or butorphanol (0.2-0.48 mg/kg). Additional anesthetics were given to effect. Total anesthetic time ranged from 60 to 420 min. Three out of four cases were treated using terbutaline (0.01 mg/kg SC), which successfully resolved the hyperkalemia. No bradyarrhythmias were seen in any cases where electrocardiography (ECG) was monitored (3/4). All cases recovered from anesthesia, with one prolonged recovery. All animals are clinically healthy at the time of writing. Factors including anesthetic duration, the use of α-2 agonists, hyperthermia, and genetics are discussed as possible triggers for hyperkalemia. Serial blood gases, with electrolyte measurements, are recommended during anesthesia of red wolves, particularly when anesthetic time may be prolonged or the patient suffers from hyperthermia. Terbutaline appears to be a successful treatment should hyperkalemia arise.
Subject(s)
Anesthetics , Hyperkalemia , Ketamine , Wolves , Animals , Hyperkalemia/drug therapy , Hyperkalemia/veterinary , Terbutaline , ButorphanolABSTRACT
OBJECTIVE: Bearded dragons (Pogona vitticeps), a popular zoological companion species, frequently require sedation for procedures. A novel formulation of alfaxalone with preservatives was FDA approved for 28-day use after the vial is breached. Research has been performed in squamate species using alfaxalone without preservatives at various doses and routes of administration, but it is unknown whether preservatives affect quality of sedation or cardiac function. ANIMALS: 10 bearded dragons. PROCEDURES: This complete crossover study evaluated the pharmacodynamic effects of alfaxalone with preservatives administered to bearded dragons via intracoelomic (ICo; n = 10), SC (10), IM (9), and IV (9) injection at 15 mg/kg. RESULTS: Deep sedation was achieved in 9 of 10 ICo, 8 of 10 SC, 8 of 9 IM, and 9 of 9 IV administrations. Heart rate significantly decreased from baseline for ICo (P = .008; median heart rate, 46), IM (P = .018; 54), and IV (P = .033; 54) routes, but maintained within clinically acceptable limits. Respiratory rate significantly decreased from baseline for ICo (P = .011; median respiratory rate, 30), SC (P = .024; 12), IM (P = .028; 12), and IV (P = .043; 12) routes. Spontaneous ventilation was retained during all events. Time to first effects was significantly sooner with IV (0 min) administration compared with ICo (P = .02; 5 min) and IM (P = .008; 5 min). Time to loss and recovery of withdrawal, righting reflex, deep pain, and purposeful movement were not significantly different between routes of administration. End-systolic volume was the only echocardiographic parameter significantly affected by IV sedation. CLINICAL RELEVANCE: Sedation quality was most consistent via IV administration at 15 mg/kg, and minimal changes in cardiac function were observed.
Subject(s)
Anesthesia , Lizards , Pregnanediones , Animals , Cross-Over Studies , Anesthesia/veterinary , Pregnanediones/pharmacology , Echocardiography/veterinaryABSTRACT
Death due to illegal trade and trafficking serves as the largest threat to pangolins in their geographic range countries. Appropriate veterinary care is essential to the welfare of sick or debilitated pangolins. Given the challenge of comprehensive physical examination of this species due to its unique anatomic features and poorly studied physiology, laboratory diagnostics can provide essential information to guide appropriate medical care. This retrospective study included previously analyzed blood and urine samples collected from adult (n = 17) African white-bellied pangolins (Phataginus tricuspis) under managed care that were sampled over a 4-yr period as part of routine veterinary examinations. Samples from clinically normal animals were used to summarize results for this species. Pangolins had a lymphocyte-predominant leukogram with a mean neutrophil to lymphocyte ratio of 0.83 (min-max: 0.13-4.81). Seasonal, sex, and age differences were present for several hematologic and serum biochemical analytes. Urine samples had a median urine specific gravity (USG) of 1.040 (min-max: 1.006-1.060), a neutral-to-basic urine pH (6.0-8.6), and contained mainly struvite crystals or infrequently calcium oxalate dihydrate crystals. Winter samples had higher USG than summer samples. The clinicopathologic data provided herein offers applicable information useful for the clinical management of pangolins.
Subject(s)
Calcium Oxalate , Pangolins , Animals , Retrospective Studies , StruviteABSTRACT
Monitoring blood lactate concentrations with a handheld, point-of-care (POC) meter is an efficient and inexpensive method of monitoring critically ill or anesthetized exotic patients. Serial monitoring of lactate allows early recognition of hypoperfusion, allowing for prompt implementation of resuscitative efforts. Reference ranges for exotic animals are currently sparse and often gathered from field studies of wild animals. In the absence of reference ranges, extrapolations can be made regarding mammals and birds, but may be more difficult in reptiles and amphibians.
Subject(s)
Animals, Exotic , Lactic Acid , Amphibians , Animals , Mammals , Point-of-Care Systems , ReptilesABSTRACT
Anesthetic drugs must be delivered at the appropriate dose and route of administration to produce the expected anesthetic effects. This is important for patient safety because anesthetic drugs function by depressing the central nervous and cardiovascular systems, which if improperly dosed or administered could cause potentially life-threatening effects. Several routes of administration and different drug delivery methods are available to safely and reliably anesthetize zoologic companion animal patients. Because of the nature of zoologic companion animal practice, anesthetic procedures pose risks that the anesthetist should understand to carefully plan procedures that are as safe and efficient as possible.
Subject(s)
Anesthetics , Pharmaceutical Preparations , Anesthetics/adverse effects , AnimalsABSTRACT
North American vipers are commonly housed in zoological institutions or studied as free-ranging populations. Because of their venomous predatory and defensive mechanism, sedation or anesthesia is frequently employed to facilitate safe handling and medical procedures, especially of the head. A new formulation of alfaxalone with proprietary preservatives was recently approved and indexed for 28-d use post-vial puncture. Pharmacodynamic effects of alfaxalone in its prior formulation have been researched in nonvenomous species, but the optimal dose and route of administration in vipers have not been reported. In part one, 10 prairie rattlesnakes (Crotalus viridis) participated in a complete four-route crossover study evaluating 20 mg/kg alfaxalone administered intracoelomically (ICo), SC cranial to the heart, IM cranial to the heart, and IV in the ventral coccygeal vein. HR significantly decreased from baseline during IV (P= 0.024), IM (P= 0.024), and SC (P= 0.028) administration. Respiratory rate significantly decreased following alfaxalone delivered IV (P = 0.027). Time to first effects was significantly faster in IV compared with IM (P= 0.01), SC (P= 0.001), and ICo (P= 0.036). All IV and IM administrations resulted in deep sedation, but 70% of the IV and 10% of the IM sedation events resulted in apnea and required intermittent positive ventilation via endotracheal tube. Fifty percent of the ICo sedation events and 10% of the SC sedation events did not result in sedation. One successful SC sedation event resulted in apnea. In part two, echocardiograms were performed in the same rattlesnakes at baseline and at maximum effect of sedation with 20 mg/kg alfaxalone administered IM. Cardiac contractility and output were unaffected. Administration of alfaxalone at 20 mg/kg IM cranial to the heart should facilitate safe handling and minimally invasive procedures in prairie rattlesnakes and related species.
Subject(s)
Anesthetics , Pregnanediones , Animals , Cross-Over Studies , Crotalus , Grassland , Injections, Intramuscular/veterinary , Pregnanediones/pharmacologyABSTRACT
OBJECTIVE: To characterize induction and recovery characteristics of 3 commonly used inhalant anesthetics in prairie rattlesnakes (Crotalus viridis): isoflurane, sevoflurane, and desflurane. ANIMALS: 12 healthy adult prairie rattlesnakes. PROCEDURES: In a randomized crossover design, snakes underwent anesthetic induction with 5% isoflurane, 8% sevoflurane, or 18% desflurane, with a washout period of ≥ 7 days between anesthetic events. Anesthetic depth parameters were recorded throughout induction and recovery, including time to loss and return of righting reflex, muscle tone, ability to intubate, response to pressure, and time to return to spontaneous respiration. Every 5 minutes throughout the anesthetic procedures, heart rate, respiratory rate, and percentage expired anesthetic gas were recorded. RESULTS: No snakes died during the study. Sevoflurane anesthesia resulted in anesthetic gas avoidance behavior in snakes during induction and had the significantly longest recovery time to extubation and time to return of pressure response, compared with the other inhalant anesthetics. Anesthesia with isoflurane resulted in a significantly longer time to return of righting reflex, compared with sevoflurane or desflurane. No significant difference was noted in time to loss of pressure response among the 3 anesthetic gases. Desflurane anesthesia resulted in the significantly quickest loss of righting reflex among the anesthetic protocols; despite this, 4 of 12 desflurane anesthetized snakes did not achieve an anesthetic plane deep enough for intubation. CONCLUSIONS AND CLINICAL RELEVANCE: Isoflurane and sevoflurane, but not desflurane, inhalation anesthesia resulted in consistent and predictable loss of righting reflex and induction of anesthesia deep enough to allow intubation in snakes.
Subject(s)
Anesthetics, Inhalation , Isoflurane , Methyl Ethers , Anesthesia Recovery Period , Anesthesia, Inhalation/veterinary , Animals , Crotalus , Desflurane , Grassland , SevofluraneABSTRACT
Assessment of pododermatitis, osteoarthritis, and other causes of lameness in penguins can be challenging. Subjective gait analysis using visual observation and response to analgesic therapy can be affected by observer variation and caregiver placebo bias. A pressure-sensitive walkway (PSW), however, allows for objective gait analysis and assessment of analgesic therapeutic response. In this study, a 3-m-long PSW was used to analyze gait in 21 adult Humboldt penguins (Spheniscus humboldti). Medical record reviews and comprehensive examinations were performed on all penguins; five penguins were considered abnormal, with either right-sided (n = 3) or bilateral historical lameness-causing disease (n = 2) and were analyzed separately from the normal data set. All penguins walked across the PSW four times and gait parameters (step and stride distances and velocities, maximum force, impulse, and peak pressure) were calculated for each foot in each penguin. Statistical comparisons were made between right and left feet, sexes, and normal and abnormal penguins for each gait parameter. Among normal penguins, there were no significant differences between feet or sex. Left step width was shorter in abnormal penguins than that of normal penguins. Study results established baseline values for Humboldt penguins. This will allow objective monitoring of progression and response to therapy in penguin lameness cases, both current and future. The data also provide a foundation to compare gait parameters with other penguin populations and species.
Subject(s)
Gait Analysis/veterinary , Gait , Spheniscidae/physiology , Animals , Animals, Zoo , Biomechanical Phenomena , Female , Gait Analysis/instrumentation , Gait Analysis/methods , MaleABSTRACT
Acute-phase proteins (APP) are the foundation to the innate immune response and valuable biomarkers that increase with inflammation, infection, neoplasia, stress, and trauma.2,4,16 Little is known about the acute-phase response in cetaceans and if these proteins can be used for health monitoring in individuals and free-ranging populations. The purpose of this study was to characterize serum concentrations of haptoglobin (Hp) and serum amyloid A (SAA), as well as electrophoretic profiles of common bottlenose dolphins (Tursiops truncatus) in free-ranging (n = 33) and professional care (n = 27) settings. Results were correlated to commonly utilized inflammatory indices including erythrocyte sedimentation rate (ESR), fibrinogen, total white blood cell count (WBC), and absolute neutrophil count. SAA levels, measured with a dolphin-specific enzyme linked immunosorbent assay (ELISA), were significantly higher (P = 0.05) in free-ranging dolphins (mean = 4.26; SE = 1.12) when compared with those under professional care (mean = 1.82; SE = 0.45). For dolphins under professional care, a statistically significant correlation was identified between ESR and Hp (P < 0.001; r = 0.69), ESR and SAA (P < 0.001; r = 0.67), fibrinogen and Hp (P = 0.001; r = 0.58), and fibrinogen and SAA (P = 0.002; r = 0.56). In addition, there was a significant correlation between WBC and SAA (P = 0.01; r = 0.38) and absolute neutrophil count and SAA (P = 0.04; r = 0.32). There were no significant correlations between study variables observed in free-ranging dolphins. The variable correlation of APPs with commonly utilized inflammatory indices demonstrates that these proteins are independent measures of inflammation with unique sensitivity, specificity, and timeline of expression. The results of this study contribute to improved health monitoring of dolphins and have the potential to assist in identification of compromised health.
Subject(s)
Blood Sedimentation , Bottle-Nosed Dolphin/blood , Fibrinogen/metabolism , Haptoglobins/metabolism , Leukocyte Count/veterinary , Serum Amyloid A Protein/metabolism , Animals , Blood Protein Electrophoresis/veterinary , Female , Florida , Male , Serum/chemistryABSTRACT
The objective of this study was to determine the accuracy of a noninvasive oscillometric method in relation to invasively measured blood pressure in anesthetized Bennett's wallabies (Macropus rufogriseus) and also to compare the accuracy of two commonly used oscillometric blood pressure monitors (manufactured by Cardell and Datascope). Eleven animals were anesthetized, and each animal was instrumented with an arterial catheter in the right medial metatarsal artery connected to a pressure transducer to obtain invasive measurements of systolic (SAP), diastolic (DAP), and mean (MAP) arterial blood pressure as well as a pressure waveform. A cuff connected to an oscillometric device was placed on the base of the tail for noninvasive measurements. Paired data from noninvasive and invasive blood pressure measurements (SAP, DAP, and MAP) were obtained every 5 min for 60 min. Bland-Altman plots were used to compare invasive and noninvasive measurements and calculate bias and 95% limits of agreement for SAP, DAP, and MAP. For both monitors, the bias of SAP, DAP, and MAP was significant, although the bias of the Cardell was consistently lower than that of the Datascope for all parameters. Limits of agreement were wide for all parameters. In conclusion, when using an oscillometric blood pressure monitor on anesthetized Bennett's wallabies, trends in blood pressure may be monitored, although all displayed readings may not represent the true blood pressure measurement. Indirect measurements of blood pressure made with the oscillometric device cannot substitute for direct measurements.
Subject(s)
Blood Pressure Determination/veterinary , Blood Pressure Monitors/veterinary , Blood Pressure/physiology , Macropodidae/physiology , Oscillometry/veterinary , Anesthesia/veterinary , Animals , Blood Pressure Determination/instrumentation , Blood Pressure Determination/methods , Female , Male , Oscillometry/instrumentationABSTRACT
Objective gait evaluation with a pressure sensitive walkway (PSW) has been used to assess welfare of poultry and to assess lameness and response to therapy in domestic mammals. Objective gait analysis of birds with lameness due to pododermatitis, osteoarthritis, and other common diseases could provide non-biased assessment and therapeutic monitoring for zoo clinicians. The objective of this study was to evaluate the use of a PSW for objective gait analysis in normal domestic ducks (Cairina moschata domestica) and those with experimentally induced arthritis. Eighteen healthy adult ducks walked across the PSW four times in each experiment at each time point. For experiment 1, gait parameters (step and stride distances and velocities, maximum force, impulse, and peak pressure) were calculated for each foot in each duck (time 0). For experiment 2, six of these ducks were randomly selected, anesthetized, and administered a unilateral intra-tarsal injection of monosodium urate solution to induce arthritis. Serial PSW trials were repeated at 1, 2, 3, 4, 8, and 24 hours post-injection. Gait parameters were calculated and compared at each time point, including baseline at time 0. Among the normal ducks, there were no significant differences between right and left feet for any gait parameter. Maximum force and impulse were significantly lower for the affected limb at the 3- and 4-hour time points in ducks with unilateral induced arthritis. This asymmetry was resolved by 8 hours post injection. This PSW transient arthritis model allows for objective assessment of lameness in domestic ducks with maximum force and impulse serving as the most sensitive gait parameters for lameness detection. This method has potential as a model to assess analgesic efficacy for zoo-housed waterfowl and other avian species.
Subject(s)
Arthritis, Experimental/physiopathology , Ducks/physiology , Gait Analysis/veterinary , Gait/physiology , Walking , Animals , Biomechanical Phenomena , Gait Analysis/instrumentation , Gait Analysis/methodsABSTRACT
OBJECTIVE To evaluate the anesthetic and cardiorespiratory effects of IM alfaxalone and isoflurane administration in budgerigars (Melopsittacus undulatus) and compare use of these agents with use of manual restraint. ANIMALS 42 healthy budgerigars. PROCEDURES For dose comparison, birds received alfaxalone (5 or 10 mg/kg [2.27 or 4.54 mg/lb], IM; groups A5 and A10, respectively; n = 6/group). For treatment comparison, birds received alfaxalone (10 mg/kg, IM) or isoflurane (via face mask) or were manually restrained (groups A, I, and M, respectively; n = 10/group). Data were obtained on onset, degree, and duration of sedation or anesthesia; heart and respiratory rates; and recovery times. Birds in the treatment comparison underwent physical examination and blood gas analysis. RESULTS All group A5 birds became sedate, but not recumbent. In group A10, 5 of 6 birds lost the righting reflex; however, none lost the noxious stimulus response. Median time to initial effects was significantly shorter and mean time to complete recovery was significantly longer in group A10 than in group A5. Heart and respiratory rates in group A10 remained clinically acceptable; however, some birds had signs of excitement during induction and recovery. Times to initial effects, recumbency, and complete recovery were significantly longer, yet clinically practical, in group A than in group I. Plasma lactate concentrations were significantly higher in group M than in groups A and I. CONCLUSIONS AND CLINICAL RELEVANCE Alfaxalone administered IM at 10 mg/kg produced effective sedation in healthy budgerigars and may be a viable alternative to isoflurane and manual restraint for brief, minimally invasive procedures. Brief manual restraint resulted in a significant increase in plasma lactate concentration.
Subject(s)
Anesthetics/pharmacology , Melopsittacus , Animals , Injections, Intramuscular/veterinary , Isoflurane , Melopsittacus/physiology , PregnanedionesABSTRACT
The purpose of this study was to determine the efficacy of tramadol and meloxicam in an induced, temporary arthritis model in ducks as assessed by ground-reactive forces measured by a pressure-sensitive walkway (PSW) system. Twelve ducks (Cairina moschata domestica) were randomly separated into 3 equal groups of 4 birds each: water control, tramadol treatment, and meloxicam treatment. Baseline measurements were collected by having all ducks walk along a 3-m-long PSW in a custom-built corral before anesthesia and induction of arthritis. Arthritis was induced in all groups through injection, under anesthesia, of a 3% monosodium urate (MSU) solution into the intertarsal joint. One hour after MSU injection, birds were orally gavage fed 1 mL of tap water (control), tramadol (30 mg/kg), or meloxicam (1 mg/kg). After treatments, all ducks were reevaluated on the PSW at 1, 2, 3, 4, 8, and 24 hours post-MSU injection. The difference in maximum force was significantly greater in the control group than in both the tramadol- (P = .006) and meloxicam-treated (P = .03) individuals. Post hoc comparisons revealed differences between control and treated birds occurred only at the 3- and 4-hour time points after administration. No differences were found in the absolute difference in maximum force between tramadol- and meloxicam-treated birds at any time point (P > .05). Results of this study support the hypothesis that tramadol (30 mg/kg PO) and meloxicam (1 mg/kg PO) improve certain objective variables in an induced arthritis model in ducks. Our findings also support studies in other avian species that determined that both tramadol and meloxicam are effective analgesic drugs in some birds.
Subject(s)
Arthritis, Experimental/veterinary , Ducks , Meloxicam/therapeutic use , Tramadol/therapeutic use , Analgesics, Opioid/therapeutic use , Animals , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Arthritis, Experimental/chemically induced , Arthritis, Experimental/drug therapy , Uric Acid/toxicity , WalkingABSTRACT
Although tricaine methanesulfonate (MS-222) immersion has historically been standard of care for fish and anuran euthanasia, recent research has proven it insufficient for euthanasia of goldfish. To assess appropriateness for humane euthanasia of anurans, this study evaluated the efficacy of MS-222 in Smokey Jungle Frogs (Leptodactylus pentadactylus). Eighteen frogs (21-33 g) were exposed to one of three MS-222 concentrations via partial immersion: 2.5 g/L for 90 min (M2.5/90), 5 g/L for 60 min (M5/60), or 10 g/L for 60 min (M10/60). Physiologic parameters and times to loss of spontaneous movement, righting reflex, and noxious stimulus response were recorded. Following exposure, frogs were rinsed with dechlorinated water, and time to cessation of heart beat was recorded. Survival in M2.5/90, M5/60, and M10/60 was one of six, zero of six, and zero of six, respectively. In M2.5/90, three of six frogs had continued purposeful, spontaneous movement throughout exposure. In M5/60 and M10/60, median (range) time to initial loss of movement was 14.3 (5.5-30.0) and 7.6 (4.8-19.7) min, respectively. Twelve of 18 frogs among all groups demonstrated a median (range) of two (one to six) episodes of regained consciousness with purposeful, spontaneous movement following loss of noxious stimulus response. Median (range) time to heart beat cessation in M2.5/90, M5/60, and M10/60 was 150 (135-210), 157.5 (60-225), and 90 (75-210) min, respectively. Although death was achieved in 17 of 18 frogs, given the repeated events of regained consciousness, MS-222 immersion when used at concentrations ≤10 g/L did not result in rapid and distress-free death and is not sufficient for humane euthanasia in this species.
Subject(s)
Aminobenzoates/therapeutic use , Anesthetics/therapeutic use , Anura/physiology , Euthanasia, Animal/methods , Animals , Dose-Response Relationship, Drug , ImmersionABSTRACT
Large arachnids are commonly managed under professional care, and anesthesia is occasionally required for physical examination and diagnostic and therapeutic procedures. Anesthetic responses and hemolymph gas analysis have been studied previously in spiders, but scorpions have yet to be investigated. This study measured hemolymph gas values with an i-STAT point of care blood gas analyzer in healthy adult Asian forest scorpions (Heterometrus longimanus = HL, n = 8) and dictator scorpions (Pandinus dictator = PD, n = 12) breathing: 1) room air (RA), 2) 100% oxygen for 10 min in a chamber (OX), and 3) 5% isoflurane and oxygen (ISO) in a chamber until induction or loss of righting reflex. All scorpions recovered without complications, and there were no cartridge failures. Analysis of hemolymph gas values revealed that pH was lower in OX compared with RA and ISO and was lower in PD compared with HL scorpions. The partial pressure of carbon dioxide did not differ between inhaled gases but was higher in PD compared with HL. The partial pressure of oxygen (pO2) was higher in ISO compared with OX, and both were higher than when breathing RA. Despite a lack of species difference in pO2, PD had a more dramatic increase in pO2 in ISO compared with HL (significant species and inhalant interaction). PD had a significantly shorter induction time than HL, but recovery time (return of righting reflex) did not differ between species. Subjectively, HL exhibited rough inductions compared with PD, characterized by violent whole-body and tail movements. The unexpected increase in pO2 in ISO compared with OX, along with the species-specific differences and anesthetic effects, emphasizes the unique respiratory physiology of scorpions and demonstrates that further species-specific studies of anesthetics are warranted.
Subject(s)
Anesthetics, Inhalation/adverse effects , Isoflurane/adverse effects , Oxygen/adverse effects , Scorpions/drug effects , Spiders/drug effects , Anesthetics, Inhalation/administration & dosage , Animals , Blood Gas Analysis/veterinary , Hemolymph/chemistry , Isoflurane/administration & dosage , Oxygen/administration & dosage , Reflex, Righting/drug effects , Scorpions/physiology , Spiders/physiologyABSTRACT
OBJECTIVE: To determine the intracoelemic (ICe) dose of alfaxalone required to induce loss of righting reflex (LRR) in garter snakes (Thamnophis sirtalis) and to evaluate the tactile stimulus response in unanesthetized and alfaxalone-anesthetized snakes. ANIMALS: 8 healthy mature garter snakes. PROCEDURES: During the first of 3 phases, snakes received each of 3 doses (10, 20, and 30 mg/kg) of alfaxalone, ICe, with a 2-week washout period between treatments. Times to LRR and return of righting reflex were determined after each dose. During phase 2, unanesthetized snakes underwent tactile stimulation testing with Semmes-Weinstein monofilaments once daily for 3 consecutive days to determine the baseline tactile pressure required to elicit purposeful movement. During phase 3, snakes were anesthetized with alfaxalone (30 mg/kg, ICe), and the tactile pressure required to induce purposeful movement was assessed at predetermined times after LRR. RESULTS: Intracoelomic administration of alfaxalone at doses of 10, 20, and 30 mg/kg induced LRR in 0, 5, and 8 snakes, respectively. For snakes with LRR, median time to LRR following the 30-mg/kg dose (3.8 minutes) was significantly shorter than that following the 20-mg/kg dose (8.3 minutes); median time to return of righting reflex did not differ between the 2 doses. Mean ± SD tactile pressure that resulted in purposeful movement in unanesthetized snakes was 16.9 ± 14.3 g. When snakes were anesthetized, the mean tactile pressure that resulted in purposeful movement was significantly increased from baseline at 10, 20, and 30 minutes after LRR. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested ICe administration of alfaxalone might be effective for anesthetizing garter snakes.
Subject(s)
Anesthetics/pharmacology , Colubridae , Pregnanediones/pharmacology , Reflex, Righting/drug effects , Anesthetics/administration & dosage , Animals , Male , Pregnanediones/administration & dosageABSTRACT
This study documents the pharmacokinetics of oral tramadol in Muscovy ducks. Six ducks received a single 30 mg/kg dose of tramadol, orally by stomach tube, with blood collection prior to and up to 24 hr after tramadol administration. Plasma tramadol, and metabolites O-desmethyltramadol (M1), and N,O-didesmethyltramadol (M5) concentrations were determined by high-pressure liquid chromatography (HPLC) with fluorescence (FL) detection. Pharmacokinetic parameters were calculated using a one-compartment model with first-order input. No adverse effects were noted after oral administration. All ducks achieved plasma concentrations of tramadol above 0.10 µg/ml and maintained those concentrations for at least 12 hr. Elimination half-life was 3.95 hr for tramadol in ducks, which is similar to other avian species. All ducks in this study produced the M1 metabolite and maintained plasma concentrations above 0.1 µg/ml for at least 24 hr.