Nitrogen Fertilization and Solvents as Factors Modifying the Antioxidant and Anticancer Potential of Arnica montana L. Flower Head Extracts.

Arnica montana L. is one of Europe's endemic endangered medicinal plants, with diverse biological activities commonly used in medicine, pharmacy, and cosmetics. Its flower heads are a rich source of raw material, with antibacterial, antifungal, antiseptic, anti-inflammatory, antiradical, antioxidant, and antitumor properties. The objective of the present study was (i) to characterize the chemical composition of flower heads of A. montana plants cultivated under nitrogen fertilization, (ii) to identify the impact of the nitrogen fertilization and extraction method (water, ethanol) on the antioxidant activity of extracts, and (iii) to determine the role of different nitrogen doses applied during plant cultivation and different extraction methods in the anticancer activity of the extracts through analysis of apoptosis and autophagy induction in HT29, HeLa, and SW620 cell lines. The present study shows that nitrogen is a crucial determinant of the chemical composition of arnica flower heads and the antioxidant and anticancer activity of the analyzed extracts. Nitrogen fertilization can modify the composition of pharmacologically active substances (sesquiterpene lactones, flavonoids, essential oil) in Arnicae flos. The content of sesquiterpene lactones, flavonoids, and essential oil increased with the increase in the nitrogen doses to 60 kg N ha-1 by 0.66%, 1.45%, and 0.27%, respectively. A further increase in the nitrogen dose resulted in a decrease in the content of the analyzed secondary metabolites. Varied levels of nitrogen application can be regarded as a relevant way to modify the chemical composition of arnica flower heads and to increase the anticancer activity, which was confirmed by the increase in the level of apoptosis with the increase in fertilization to a level of 60 kg N ha-1. The fertilization of arnica plants with low doses of nitrogen (30 and 60 kg N ha-1) significantly increased the LOX inhibition ability of the ethanol extracts. The present study is the first report on the anticancer activity of A. montana water extracts, with emphasis on the role of water as a solvent. In further studies of factors modifying the quality of Arnicae flos, attention should be paid to the simultaneous use of nitrogen and other microelements to achieve synergistic results and to the possibility of a more frequent use of water as a solvent in studies on the biological activity of A. montana extracts.

Effects of biotransport and hydro-meteorological conditions on transport of trace elements in the Scott River (Bellsund, Spitsbergen).

The shaping of surface water chemistry in the Svalbard Archipelago is strongly dependent on the geology of the catchment and the process of long-range transport of atmospheric pollutants (LRATP). It was found that the dissolved trace elements in the Scott River, which catchment is characterized by a decreasing degree of glaciation, were of the natural origin (i.a. weathering and dissolution of local geological substratum). The exception was Zn originated from LRATP. The paper describe the influence changes in hydro-meteorological conditions and the presence of a seabird colony on the variability in the transport of trace elements within the Scott River catchment. The work assesses long-time fluctuations in the concentration of twenty five trace elements (i.a. Al, Cr, Cu, Pb, Sr, and Zn) from eighty-four surface water samples and their relation to changes in water discharge (Q), precipitation (P), pH, and dissolved organic carbon (DOC) at two river sites (with one being under the influence of the biotransport factor). Based on the results of matrix correlation and cluster analysis it was found that the additional load of DOC from the nesting site of Larus Argentatus in the mouth section of the river drastically changed the hydro-geochemical cycle of Co, Ni, Zn, Ga, Sr, Rb, Ba and U (0.30 < r < 0.51). Furthermore, the results of cluster analysis confirmed that the bird colony's nesting site was strongly responsible for the presence of U, Rb, Zn, Ni and marine-derived nutrients (e.g. Se and Li). The discharge of glacier meltwater and the alkaline character of water have a negative effect on the dissolution of Li and Mn (-0.31 < r < -0.51), but positively affect the level of Rb and U (r = 0.31 and 0.35, respectively) due to it being washing out a seabird nesting colony in the mouth section of the Scott River. It was observed that the event of rises in air temperature and rain, which results in increased water discharge, caused an intense transport of the trace elements load. Moreover, results of the precipitation sensitivity coefficient factor (CF) proved that precipitation effect the occurrence of Li, Sr and U in the Scott River.

First evaluation of wastewater discharge influence on marine water contamination in the vicinity of Arctowski Station (Maritime Antarctica).

In Antarctica, waste is generated mainly during scientific research programmes and related logistics. In this study, the impact of wastewater on the western shore of Admiralty Bay was investigated during austral summer in 2017 and 2019. A range of physicochemical parameters and the presence of selected trace metals, formaldehyde and different groups of surfactants were determined in wastewater coming from Arctowski Station and in nearby coastal waters. The presence of selected trace metals (e.g., Cr: 2.7-4.4 µg/L; Zn: 15.2-37.3 µg/L; and Ni: 0.9-23.3 µg/L) and the sums of cationic (0.3-1.5 mg/L), anionic (3.1-1.7 mg/L), and non-ionic (0.6-2.4 mg/L) surfactants in wastewater indicated the potential influence of anthropogenic factors on sea water. The determined surfactants are found in many hygiene products that end up in the waste water tank after human use and, if untreated, can be released into surface waters with discharge. In addition, the levels of some trace metals indicate that they cannot come only from natural sources, but are the result of human activity. The reported data show disturbances in the marine environment caused by non-treated wastewater discharge, e.g. by comparing the obtained results from the values of the no observed effect concentrations (NOECs) on selected Antarctic bioindicators, and provide information for the implementation of proper wastewater treatment at any Antarctic station in the future.

Seashore sediment and water chemistry at the Admiralty Bay (King George Island, Maritime Antarctica) - Geochemical analysis and correlations between the concentrations of chemical species.

This study covers water and sediment chemical characteristics by the western shore of Admiralty Bay (King George Island, Antarctica) in 2016. Chemical processes between sediment and water have been described based on the determination of ions, metals, non-metals, and TOC concentrations. Rock weathering is an important source of Fe, Ni, Co, Al in the seashore area. The PCA shows the impact of acidification in the release metals from sediment. Our results indicate that riverine mineral fluxes need to be accounted for as the volume of melt increases in response to climate change. Based on geoaccumulation indexes (anthropogenic fingerprint), we observed an increased concentration of Pb (Igeo = 1.643), in the lake near station facilities and Cd in the area of Ecology Glacier (Igeo > 1.389). Taking into account climate change and the intensification of anthropopressure, our study indicates that Antarctica requires a special focus on the seasonal dynamics of mineral content and pollution assessment.

Environmental characteristics of a tundra river system in Svalbard. Part 2: Chemical stress factors.

Bacterial communities in the Arctic environment are subject to multiple stress factors, including contaminants, although typically their concentrations are small. The Arctic contamination research has focused on persistent organic pollutants (POPs) because they are bioaccumulative, resistant to degradation and toxic for all organisms. Pollutants have entered the Arctic predominantly by atmospheric and oceanic long-range transport, and this was facilitated by their volatile or semi-volatile properties, while their chemical stability extended their lifetimes following emission. Chemicals present in the Arctic at detectable and quantifiable concentrations testify to their global impact. Chemical contamination may induce serious disorders in the integrity of polar ecosystems influencing the growth of bacterial communities. In this study, the abundance and the types of bacteria in the Arctic freshwater were examined and the microbial characteristics were compared to the amount of potentially harmful chemical compounds in particular elements of the Arctic catchment. The highest concentrations of all determined PAHs were observed in two samples in the vicinity of the estuary both in June and September 2016 and were 1964 ng L-1 (R12) and 3901 ng L-1 (R13) in June, and 2179 ng L-1 (R12) and 1349 ng L-1 (R13) in September. Remarkable concentrations of the sum of phenols and formaldehyde were detected also at the outflow of the Revelva river into the sea (R12) and were 0.24 mg L-1 in June and 0.35 mg L-1 in September 2016. The elevated concentrations of chemical compounds near the estuary suggest a potential impact of the water from the lower tributaries (including the glacier-fed stream measured at R13) or the sea currents and the sea aerosol as pollutant sources. The POPs' degradation at low temperature is not well understood but bacteria capable to degrading such compounds were noted in each sampling point.

Annual variability of heavy metal content in Svalbard reindeer faeces as a result of dietary preferences.

During both winter and summer, Svalbard reindeer selectively feed on different types of vegetation that are not only a source of nutritional value, but also a place of heavy metal accumulation. In the present study, the content of cadmium, chromium, copper, iron, lead, nickel, manganese, and zinc in reindeer excrement was measured. The main aims were to determine the seasonal content of several heavy metals in Svalbard reindeer faeces, and to compare their values in terms of dietary preferences during the year. Summer and winter reindeer excrement was gathered along a designated linear transect running through Bolterdalen and the vegetation described on 1 m2 plots. All of the analysed heavy metals were detected in the reindeer faeces and this fact seems to be connected with the incomplete content of these elements in an animal's tissue after forage digestion. Analysis showed differences between summer and winter excrement in terms of concentrations of cadmium, chromium, iron, and nickel, but no differences were found for the other four elements analysed (manganese, lead, zinc, and copper). However, concentrations of heavy metals in faeces are rather low in comparison with both the levels in the vegetation that may be grazed by reindeer and in reindeer tissue.

Sweat iron concentration during 4-week exercise training.

INTRODUCTION: One possible way of iron loss is sweating. It is unclear how physical activity performed by untrained individuals affects the iron status in sweat. OBJECTIVE: The purpose of this study was to analyse iron concentration in sweat during 4-week exercise training to determine the changes in iron excretion during follow-up exercises. MATERIAL AND METHODS: 43 untrained volunteers participated in the study, 29 of whom completed the full exercise programme. The training programme consisted of exercises on a cycle ergometer and cross-trainer. In the first week, participants exercised for 8 minutes on each device, in the second for 10 minutes, and in the third and fourth weeks they exercised for 15 min on each device. Intensity was submaximal and defined as 85% of maximal heart rate. A sterile sweat patch was placed on the skin between shoulder blades. RESULTS: Concentration of iron on the first and the fifteenth day of exercises was comparable and statistically insignificant. Iron concentration was highly increased on the last day of training in comparison with first (p<0.001) and fourteenth day (p<0.006). The median of iron concentration in 29 samples on the first day of sampling was 21.2 ppb, in the fifteenth - 52.5 ppb, and on the twenty-eighth day - 286.2 ppb. In relation with the sodium concentration, the iron content was also increased on the twenty-eighth day of the training programme (p<0.005). CONCLUSIONS: Iron sweat loss significantly increased during the 4-week exercise programme. A possible explanation may be improvement in the thermoregulation mechanism and secretory activity of sweat glands. Iron sweat loss may be an indicator of iron deficiency observed in active individuals.

Svalbard reindeer as an indicator of ecosystem changes in the Arctic terrestrial ecosystem.

Over the years, noticeable effort has been directed towards contaminant determination in multiple biotic samples collected from the inhabitants of the Arctic. Little consideration has been given to polar herbivores, however, especially those from the European parts of the Arctic. To provide a broader perspective, we aimed to decipher trace element concentration in hairs of the key species in the Arctic, namely the Svalbard reindeer (Rangifer tarandus platyrhynchus), and to recognise whether diet variations could correspond with forward exposure. The effect of habitat and diet was investigated using the ratios of stable isotopes of carbon (δ13C) and nitrogen (δ15N), and previous literature studies on vegetation from the areas of interest. Analysis was performed for eighteen elements in total, both toxic and essential. Metals were present in a decreasing order Fe > Zn > Ba > Cu > Pb > Cr > Ni > V > Ga =La > Rb > As > Li > Co > Hg > Cd > Cs > Be. Similarity in trends in the studied subpopulations was observed for many metals. A significant log-linear correlation was observed for most of the elements, excluding nitrogen and carbon isotopes signature. Extremely high iron levels were determined in some of the samples, suggesting past iron overload. Zinc, in contrast to the remaining metals, did not correlate well with any other element. Mercury was determined at very low levels, in accordance with previous literature regarding its concentrations in moss and lichen species in Svalbard. The analysis of stable isotopes showed a high variation in nitrogen isotopes signatures. Further research is required to properly evaluate the potential health risks and ecological implications of elevated exposure.

Comparison of hydrochemistry and organic compound transport in two non-glaciated high Arctic catchments with a permafrost regime (Bellsund Fjord, Spitsbergen).

An increase in air temperature related to climate change results in the retreat of glaciers, the degradation of permafrost, and the expansion of glacier-free areas in the polar regions. All these processes lead to changes in the Arctic landscape. They influence the hydrochemistry of streams and rivers fed by glaciers and thawing permafrost. In this study, we examine eighty two water samples from two non-glaciated catchments with snow-permafrost regime: the Tyvjobekken Creek and the Reindeer Creek (NW Wedel-Jarlsberg Land, Spitsbergen). We cover hydrometeorological measurements, fluctuations of physicochemical parameters (pH, specific electrolytic conductivity (SEC)), and the presence of selected organic compounds (dissolved organic carbon (DOC), formaldehyde (HCHO), ∑phenols). The obtained levels of DOC (0.061-0.569mgCL-1) and HCHO (0.40). The Tyvjobekken Creek catchment, in contrast to Reindeer Creek catchment, turned out to be resistant to rapid changes in meteorological conditions (r<0.10) and surface runoff. The processes of permafrost thawing, calcium carbonate dissolution, and biogeochemical "breathing" of soils proved to be crucial for the development of water chemistry. In conclusion, the surface water chemistry of the Reindeer Creek was found to result from the mutual influence of hydrometeorological indices and the biogeochemical environment of the catchment.

The interaction between bacterial abundance and selected pollutants concentration levels in an arctic catchment (southwest Spitsbergen, Svalbard).

Persistent organic pollutants (POPs) have been a topic of interest in environmental sciences for >60years. POPs in the Arctic have been investigated since the 1970s, when first atmospheric measurements revealed the presence of these pollutants in the polar regions. Major contaminant transport routes to the Arctic include atmospheric and oceanic transport, as well as inflow from rivers and sea ice. The sources of pollutants, such as industry, power generators, vehicle and ship exhausts, introduce the PAHs, phenols, formaldehyde or metals into the Arctic. Transport via sea currents, however, can take several years. The highest concentration levels of total PAHs were observed in two samples from the tributaries in July 2015 and were 1069ngL-1 and 3141ngL-1 and in September 2015, the highest concentrations were observed in samples collected from Revvatnet lake and were 978ngL-1 and 1823ngL-1. The highest concentrations of trace elements in both months were 41µgL-1 in the sample from the highest tributary (July 2015) and 79µgL-1 in the same sample (September 2015). The purpose of this study was also to determine abundance of bacteria in the Arctic freshwater of different types. Microbes are omnipresent and represent diverse biological communities. In the freshwater ecosystems, microorganisms form the base of the food chain supporting higher trophic levels. Although microbes are generally thought to live in the warm regions of Earth, many of them develop in cold climates. In the Revelva catchment, the biggest number of bacteria were detected at the river estuary in July 2015 and at the sampling point located in the Revvatnet lake in September 2015. Generally, the bacterial abundance indices depended on nutrient levels to a small extent, showing the environment of the Revelva catchment not to be nutrient limited, which is in accordance with its rich biological life also in macroscale.

Water chemistry of tundra lakes in the periglacial zone of the Bellsund Fiord (Svalbard) in the summer of 2013.

Climate changes observed in the Arctic (e.g. permafrost degradation, glacier retreat) may have significant influence on sensitive polar wetlands. The main objectives of this paper are defining chemical features of water within six small arctic lakes located in Bellsund (Svalbard) in the area of continuous permafrost occurrence. The unique environmental conditions of the study area offer an opportunity to observe phenomena influencing water chemistry, such as: chemical weathering, permafrost thawing, marine aerosols, atmospheric deposition and biological inputs. In the water samples collected during the summer 2013, detailed tundra lake water chemistry characteristics regarding ions, trace elements, pH and specific electrolytic conductivity (SEC25) analysis were determined. Moreover, water chemistry of the studied lakes was compared to the water samples from the Tyvjobekken Creek and precipitation water samples. As a final step of data analysis, Principal Component Analysis (PCA) was performed. Detailed chemical analysis allowed us to conclude what follows: (1) Ca2+, Mg2+, SO42-, Sr are of geogenic origin, (2) NO3- present in tundra lakes and the Tyvjobekken Creek water samples (ranging from 0.31 to 1.69mgL-1 and from 0.25 to 1.58mgL-1 respectively) may be of mixed origin, i.e. from biological processes and permafrost thawing, (3) high contribution of non-sea-salt SO42->80% in majority of studied samples indicate considerable inflow of sulphate-rich air to the study area, (4) high content of chlorides in tundra lakes (range: 25.6-32.0% meqL-1) indicates marine aerosol influence, (5) PCA result shows that atmospheric transport may constitute a source of Mn, Co, Ni, Cu, Ga, Ba and Cd. However, further detailed inter-season and multi-seasonal study of tundra lakes in the Arctic are recommended. Especially in terms of detailed differentiation of sources influence (atmospheric transport vs. permafrost degradation).

Impact of a newly-formed periglacial environment and other factors on fresh water chemistry at the western shore of Admiralty Bay in the summer of 2016 (King George Island, Maritime Antarctica).

This study provides a comprehensive analysis of the inorganic chemistry of flowing water at the western shore of Admiralty Bay. In the water samples, ions, and major and trace metals (and B) were detected and quantified. Additionally, the parameters of pH, specific electrolytic conductivity (SEC25) and total organic carbon (TOC) were determined. Moreover, multivariate data set was created and Principal Component Analysis (PCA) was performed. Generally, the water has low total content of the measured mineral constituents <100mgL-1. PCA analysis we distinguished two groups of chemical variables shaping water chemistry in the investigated creeks: I - components of marine aerosol origin (presence of Na+, Cl- and B) and II - those associated with chemical weathering processes (presence of Al and Fe). Furthermore, the results showed that the flowing water in the newly-formed periglacial areas (formed over the last 30years) are rich in easily soluble Al and Fe and have lower total measured contents of mineral constituents during the summer period than creeks in non-glacial catchments. Permafrost influence on water chemistry is difficult to identify. The rather insignificant difference between TOC concentrations in waters during summer indicates that permafrost is not a store of organic matter in the studied area. Moreover, local biological factors (lichens and mosses) and those limited to the sea-shore vicinity (seabirds and mammals activity) are significant sources of PO43-, NO3-, and NH4+. Despite the described geological and biological features influencing water chemistry, the impact of anthropogenic activity still needs to be verified, especially in terms of heavy metal concentrations.

Do morphometric parameters and geological conditions determine chemistry of glacier surface ice? Spatial distribution of contaminants present in the surface ice of Spitsbergen glaciers (European Arctic).

The chemism of the glaciers is strongly determined by long-distance transport of chemical substances and their wet and dry deposition on the glacier surface. This paper concerns spatial distribution of metals, ions, and dissolved organic carbon, as well as the differentiation of physicochemical parameters (pH, electrical conductivity) determined in ice surface samples collected from four Arctic glaciers during the summer season in 2012. The studied glaciers represent three different morphological types: ground based (Blomlibreen and Scottbreen), tidewater which evolved to ground based (Renardbreen), and typical tidewater glacier (Recherchebreen). All of the glaciers are functioning as a glacial system and hence are subject to the same physical processes (melting, freezing) and the process of ice flowing resulting from the cross-impact force of gravity and topographic conditions. According to this hypothesis, the article discusses the correlation between morphometric parameters, changes in mass balance, geological characteristics of the glaciers and the spatial distribution of analytes on the surface of ice. A strong correlation (r = 0.63) is recorded between the aspect of glaciers and values of pH and ions, whereas dissolved organic carbon (DOC) depends on the minimum elevation of glaciers (r = 0.55) and most probably also on the development of the accumulation area. The obtained results suggest that although certain morphometric parameters largely determine the spatial distribution of analytes, also the geology of the bed of glaciers strongly affects the chemism of the surface ice of glaciers in the phase of strong recession.