Fat Juice: A Novel Approach on the Usage and Preparation of Adipose Tissue By-Products.

BACKGROUND: Adipose tissue is considered to be naturally rich in a range of bioactive substances that may be extracted directly for therapeutic use without the need for cell isolation or culture. OBJECTIVES: The aim of this study was to introduce a novel approach that utilizes stromal vascular fraction in conjunction with fat extract, termed "fat juice," and to perform a comprehensive biochemical analysis in relation to the potential clinical relevance of this new combination. METHODS: A total of 11 samples of fat juice from the abdominal lipoaspirate were extracted from 11 healthy patients and analyzed in terms of the quantity and viability of stem cells, the presence and quantification of connective tissue fibers on histopathologic examination, and the levels of interleukin-6, mannose receptor C type 1, and vascular endothelial growth factor measured by enzyme-linked immunosorbent assay. RESULTS: Total stem cell amounts ranged from 0.14 × 105 to 1.31 × 105, and cell viability rates varied between 20% and 67.9%. Interleukin-6 protein and vascular endothelial growth factor expressions were highest in Sample 3, while staining intensity was highest in Sample 4. For collagen I, collagen III, and elastin, the highest expressions were observed in Samples 4 and 8, in Sample 3, and in Samples 2 and 4, respectively. CONCLUSIONS: Fat juice provides an easy-to-inject concentration of adipocyte/preadipocytes, red blood cells, adipose-derived stem cells, endothelial-derived cells, and cell residues. Prepared through an easy isolation process enabling abundant availability, fat juice seems to be an effective skin quality enhancer with potential for widespread use in the fields of plastic surgery, dermatology, and aesthetic/regenerative medicine.

360 Genital Fat Transfer.

BACKGROUND: This study was designed to evaluate utility of transferring autologous adipose-derived mesenchymal stem cells with high regenerative capacity and adipose tissue derived-stromal vascular fraction, so-called 360 Vaginal Beautification technique, in labia majora augmentation and vaginal tightening operation. METHODS: A total of 97 female patients who underwent labia majora augmentation and vaginal tightening operation with 360 Vaginal Beautification technique were included. Post-discharge early (3rd and 7th postoperative day) and late (1st and 3rd postoperative month) surgical complications were assessed , while the Female Genital Self-Image Scale (FGSIS) was applied before surgery and also during postoperative 6-12 months. RESULTS: All complications noted on postoperative 3rd day (ecchymosis of labia majus, ecchymosis of clitoral hood, tenderness in the pubic area and pain at the vaginal entrance points) regressed on postoperative 7th day with no infection, edema, lipoma or granuloma formation in any patient. Total mean FGSIS score was 17.7 ± 1.6 in the pre-operative period, and increased significantly to 20.9 ± 1.4 and 22.2 ± 1.8 in the postoperative 6th month (p < 0.001) and 12th month (p = 0.013), respectively. CONCLUSIONS: The use of autologous fat, called 360 vaginal beautification, in the labia majora augmentation and vaginal tightening appears to be a safe technique due to use of autologous tissue transfer and to be associated with high satisfaction rate and an advantage of being more minimally invasive than surgical labia majora augmentation and vaginal tightening. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine Ratings, please refer to Table of Contents or online Instructions to Authors www.springer.com/00266 .

Genital beautification: a concept that offers more than reduction of the labia minora.

BACKGROUND: The interest and demand for female genital rejuvenation surgery are steadily increasing. This report presents a concept of genital beautification consisting of labia minora reduction, labia majora augmentation by autologous fat transplantation, labial brightening by laser, mons pubis reduction by liposuction, and vaginal tightening if desired. METHODS: Genital beautification was performed for 124 patients between May 2009 and January 2012 and followed up for 1 year to obtain data about satisfaction with the surgery. RESULTS: Of the 124 female patients included in the study, 118 (95.2 %) were happy and 4 (3.2 %) were very happy with their postoperative appearance. In terms of postoperative functionality, 84 patients (67.7 %) were happy and 40 (32.3 %) were very happy. Only 2 patients (1.6 %) were not satisfied with the aesthetic result of their genital beautification procedures, and 10 patients (8.1 %) experienced wound dehiscence. CONCLUSION: The described technique of genital beautification combines different aesthetic female genital surgery techniques. Like other aesthetic surgeries, these procedures are designed for the subjective improvement of the appearance and feelings of the patients. The effects of the operation are functional and psychological. They offer the opportunity for sexual stimulation and satisfaction. The complication rate is low. Superior aesthetic results and patient satisfaction can be achieved by applying this technique. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Effects of Lipokit® centrifugation on morphology and resident cells of adipose tissue / Efectos de la centrifugación de Lipokit® sobre la morfología y las células residentes del tejido adiposo

The aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical soft tissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy and unreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fundamental stepin the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural properties after with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique. Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a micro filter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. These slides were stained with H&E and Toluidine Blue. Paraffin sections were immunohistochemically stained with CD34, von Willebrand Factor, CD73, CD90 and CD105. Smear preparations showed a continuous three dimensional plasma membrane appearance of adipocytes. C+ and C- showed expression of CD34, von Willebrand Factor, CD73, CD90, CD105. C+ seems to have more free cells expressing than C-. While passing the filter of Lipokit, large adipocytes and connective tissue parts disintegrate and thus increases the surface area of lipoaspirate. Lipokit® machine release the group cells which are necessary for angiogenesis and they become more freely to construct angiogenesis.

El objetivo de la ingeniería del tejido adiposo es la creación de tejido graso vascularizado autólogo para ser utilizado en clínica humana para la reconstrucción de tejido blando. Desafortunadamente en la práctica, los resultados a largo plazo del trasplante de grasa son poco fiables y no seguros; para superar este problema, se han desarrollado en diferentes países, en los últimos 20 años, variadas técnicas de lipoinyección. La centrifugación es un paso fundamental en la preparación del tejido adiposo. Nos hemos centrado en algunos marcadores, especialmente, de células precursoras mesenquimales y propiedades histológicas estructurales después de la centrifugación mediante Lipokit y sin la centrifugación por Lipokit del tejido adiposo obtenido mediante liposucción. El tejido adiposo fue tomado por liposucción y se separó en dos porciones. Una se centrifugó mediante el sistema Lipokit (C +), con un microfiltro y la otro no (C-). Después de centrifugación, muestras del frotis y secciones de parafina se prepararon a partir de estos tejidos. Los frotis se tiñeron con H&E y azul de toluidina. Las secciones de parafina se tiñeron inmunohistoquímicamente con CD34, factor de von Willebrand, CD73, CD90 y CD105. Las preparaciones de los frotis mostraron una apariencia tridimensional continua de la membrana plasmática de los adipocitos. Tanto en C+ y C- se observó la expresión de CD34, factor de von Willebrand, CD73, CD90 y CD105. En C+ parecen expresarse más células libres que en C-. Cuando se utilizó el filtro de Lipokit, los adipocitos grandes y partes del tejido conectivo se desintegraron, por lo tanto aumentó el área de superficie de lipoaspirado. El sistema Lipokit® libera los grupos celulares que son necesarios para la angiogénesis y se hacen más libres para promoverla.