ABSTRACT
This is the first record on literature to use biochar as support for CoFe2O4 to applicate and evaluate it as photocatalyst for degradation of organic pollutants. The support was verified by XRD, FT-IR, SEM, EDS and band gap. Composites CFO1BQ3, CFO1BQ1, and CFO3BQ1 showed 100% degradation in 60â min. This outstanding performance can be related to the drop in band gap energy and recombination rate of e¯/h + . The composites showed better efficiency when compared to pure CoFe2O4 (â¼78%). This might be associate to the fact that biochar has a high concentration of phenolic, hydroxyl and carboxylic functional groups on its surface. In this reaction h+, O2â¢-, and â¢OH were the reactive species involved in the degradation. The toxicity of ponceau was tested before and after the treatment, through biochemical biomarkers in Danio rerio fish. In general, the treatment proved to be efficient in reducing ponceau toxicity in D. rerio fish.
ABSTRACT
Atrazine (ATZ) is one of the pesticides mostly widely used in Brazil; several studies have shown the toxic effects of this herbicide on aquatic organisms such as fish. Thus, it is absolutely necessary finding alternatives to protect the health of fish, mainly of species commercially important for aquaculture, which may be exposed to atrazine deriving from agricultural runoff. The aim of the current study was to investigate interactions between dietary supplementation with vitamin C (Vit C) antioxidant and exposure to ATZ in Rhamdia quelen fish exposed to this herbicide. R. quelen specimens were divided into four groups: (1) CTRL, (2) VitC, (3) ATZ, (4) ATZ + VitC. Groups 3 and 4 were exposed to ATZ (10 µg L-1) for 96 h, after 30 days of VitC supplementation (1 g kg-1). Liver samples were collected for biomarker assays. Group 4 was the only group presenting decreased protein carbonyl content. Non-protein thiol (NPSH) levels were significantly higher in groups VitC, ATZ and ATZ + VitC than in CTRL. Group ATZ + VitC presented significant increase in glutatione-peroxidase (GPx) activity in comparison to the other investigated groups. Ascorbic acid (AA) levels were significantly higher in group VitC and lower in group ATZ. Therefore, interactions between herbicide ATZ and dietary supplementation with Vit C have shown biochemical changes in R. quelen fish. Thus, dietary supplements with adequate amounts of Vit C can be added as antioxidants to the diet of fish bred in aquaculture systems in order to protect them from exposure to ATZ.
Subject(s)
Atrazine , Catfishes , Herbicides , Water Pollutants, Chemical , Animals , Antioxidants/metabolism , Ascorbic Acid , Atrazine/toxicity , Catfishes/metabolism , Dietary Supplements , Herbicides/toxicity , Protein Carbonylation , Water Pollutants, Chemical/metabolism , Water Pollutants, Chemical/toxicityABSTRACT
The aim of the current study is to investigate whether silver catfish (Rhamdia quelen) individuals exposed to commercial formulation of the chlorantraniliprole insecticide used in rice crops present changes in biochemical parameters. Fifty-four (54) silver catfish individuals were distributed in six units per tank (n = 6/repetition; triplicate/treatment) and subjected to the following treatments: T1-control, without insecticide; T2 (0.02 µg/L of insecticide) and T3 (0.20 µg/L of insecticide). Exposure time lasted 24 or 96 h, and it was followed by 96 h recovery in pesticide-free water. Results have indicated biochemical changes in cortisol, glucose, lactate and plasma protein levels, as well as few ionic changes in animals' gills during the exposure and recovery periods. Chlorantraniliprole incidence in water resulted in some biochemical changes in silver catfish specimens' plasma and gills throughout the acute exposure protocol (sub-lethal dose). Thus, chlorantraniliprole insecticide has caused osmoregulatory and/or biochemical imbalance in the investigated species under the herein adopted laboratory conditions; these changes did not get back to normal levels even after specimens were left to recover for 96 h in clean water.
Subject(s)
Catfishes , Insecticides , Animals , Gills , Humans , Insecticides/toxicity , ortho-Aminobenzoates/toxicityABSTRACT
Imidacloprid (IMI) is an insecticide used worldwide, a neonicotinoid that could cause toxicity in non-target organisms. Zebrafish (Danio rerio) is a model organism widely used in different fields of research such as behavioral studies, biochemical parameters as well as neurotoxicity research. Here, we investigate whether the exposure to three concentrations (0.15, 15, and 45 µg/L) of IMI for 96 h alters responses in zebrafish. Oxidative stress parameters and acetylcholinesterase activity (AChE) as well as the behavioral responses of locomotion were measured. IMI exposure decreased distance traveled in fish exposed to the 45 µg/L. In the exploratory activity, time spent and transitions to the top area of the water column decreased in fish exposed to all concentrations of IMI. In addition, exposures to 45 and 15 µg/L of IMI decreased episodes of erratic movement in the zebrafish. Exposures to IMI at a concentration of 45 µg/L decreased the time spent in erratic movements and increased the time spent with no movement (i.e., "freezing"). Glutathione S-transferase (GST) activity was increased in the brain of zebrafish exposed for 96 h to concentrations of 0.15 and 45 µg/L. Brain AChE activity was reduced and the levels of carbonyl protein (CP) increased in brain of zebrafish at concentrations of 15 and 45 µg/L. Lipid peroxidation measured by TBARS and, also non-protein thiols (NPSH) did not show any variation in the brain of zebrafish exposed to IMI. Changes in the activity of cholinergic neurotransmitters in the brain tissues of zebrafish indicate IMI toxicity. Exposures of fish over 96 h to IMI at a nominal concentration of 45 µg/L caused more extensive sublethal responses in zebrafish, but this concentration is well above those expected in the aquatic environment. Studies are warranted to evaluate the effects on behavior and biomarker responses in fish exposed over longer periods to IMI at environmentally relevant concentrations.
Subject(s)
Water Pollutants, Chemical , Zebrafish , Animals , Antioxidants , Neonicotinoids/toxicity , Nitro Compounds , Oxidative Stress , Water Pollutants, Chemical/analysis , Water Pollutants, Chemical/toxicityABSTRACT
The disposal of untreated sanitary sewage in the soil has several consequences for human health and leads to environmental risks; thus, it is necessary investigating, monitoring and remediating the affected sites. The aims of the current study are to evaluate ecotoxicological effects on Eisenia andrei earthworms exposed to soil subjected to sources of sanitary sewage discharge and to investigate whether prevention values established by the Brazilian legislation for soil quality, associated with the incidence of chemical substances in it, are satisfactory enough to assure the necessary quality for different organisms. Earthworms' behavior, reproduction, acetylcholinesterase activity, catalase, superoxide dismutase and malondialdehyde levels were evaluated. The reproduction and behavior of earthworms exposed to sanitary sewage were adversely affected. Increased superoxide dismutase and catalase activity acted as antioxidant defense mechanism. Significantly increased lipid peroxidation levels and acetylcholinesterase activity inhibition have indicated lipid peroxidation in cell membrane and neurotransmission changes, respectively. Results have confirmed that sanitary sewage induced oxidative stress in earthworms. In addition, based on biochemical data analysis, the integrated biomarker response (IBR) has evidenced different toxicity levels in earthworms between the investigated points. Finally, results have indicated that effluents released into the soil, without proper treatment, lead to contaminant accumulation due to soil saturation and it can hinder different processes and biological development taking place in the soil. In addition, the current study has shown that physical-chemical analyses alone are not enough to assess soil quality, since it is also requires adopting an ecotoxicological approach. Brazilian legislation focused on soil quality must be revised and new guiding values must be proposed.
Subject(s)
Oligochaeta/physiology , Soil Pollutants/analysis , Animals , Antioxidants/metabolism , Brazil , Catalase/metabolism , Ecotoxicology , Environmental Pollution/analysis , Humans , Malondialdehyde/metabolism , Oligochaeta/drug effects , Oxidative Stress/drug effects , Sewage , Soil/chemistry , Superoxide Dismutase/metabolismABSTRACT
The health of family farmers is at risk due to occupational exposure to pesticides. The aims of the current study were to investigate the level of farmers' perception of risks associated with pesticide use and to assess their health condition based on biochemical and immunological tests. Family farmers living in a vegetable-producing region in Southern Brazil were selected to participate in the study. More than 70% of the family farmers were often exposed to more than one type of pesticides; 41.2% were intensively using several pesticides for more than one decade and 74.4% were not using personal protective equipment (PPE) at the time of pesticide handling due to low perception of the risks posed by these chemicals. Enzymatic analysis performed in participants' blood samples showed changes in catalase (CAT) and glutathione reductase (GR) activity, in lipid peroxidation (TBARS) and carbonylated protein levels, as well as in chemoattractant (IL-8) and anti-inflammatory (IL-10) interleukin expression. Low perception of health-related risks posed by pesticides can be attributed to factors such as low schooling and lack of information, which put farmers' health at risk, as evidenced by blood biochemical and immunological changes.
Subject(s)
Occupational Exposure , Pesticides , Agriculture , Brazil , Farmers , Health Knowledge, Attitudes, Practice , Humans , VegetablesABSTRACT
Antibiotics residues (AR), antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) are a new class of water contaminants, due to their adverse effects on aquatic ecosystems and human health. Contamination of water bodies occurs mainly by the excretion of antibiotics incompletely metabolized by humans and animals and is considered the main source of contamination of antibiotics in the environment. Given the imminent threat, the World Health Organization (WHO) has categorized the spread of antibiotics as one of the top three threats to public health in the twenty-first century. The Urban Wastewater Treatment Plants (UWWTP) bring together AR, ARB, ARG, making the understanding of this peculiar environment fundamental for the investigation of technologies aimed at combating the spread of bacterial resistance. Several methodologies have been employed focusing on reducing the ARB and ARG loads of the effluents, however the reactivation of these microorganisms after the treatment is widely reported. This work aims to elucidate the role of UWWTPs in the spread of bacterial resistance, as well as to report the efforts that have been made so far and future perspectives to combat this important global problem.
Subject(s)
Bacteria/drug effects , Bacteria/genetics , Drug Resistance, Bacterial/genetics , Drug Resistance, Microbial , Genes, Bacterial , Wastewater/microbiology , Anti-Bacterial Agents/pharmacology , Bacteria/isolation & purification , Drug Resistance, Microbial/genetics , Genes, Bacterial/genetics , Water PurificationABSTRACT
Brazilian freshwater ecosystems are continuously exposed to pesticides and domestic sewage. The Uruguay River was chosen for this study because of its international importance, as it flows through Brazil, Argentina, and Uruguay. It receives contaminants such as pesticides and domestic residues. Thus, the aim of this study to assess the accumulation of pesticides in muscle of the fish Astyanax jacuhiensis, its biochemical responses, and the presence of pesticides in water. In total, seven pesticides were registered in water from both river sites. Eight pesticides were detected in fish muscle. The biochemical responses showed that brain lipid peroxidation (LPO) and protein carbonyl (PC) in A. jacuhiensis were higher in the summer. Muscle showed the highest LPO levels in the spring and the highest PC in the summer. Liver LPO and PC levels were higher in the spring and summer. In the gills, the PC was higher in the spring and the LPO in the spring and winter. In the brain and in the gills, glutathione-S-transferase activity was high in the summer and autumn. Catalase activity was lower during the winter and spring. Non-protein thiol (NPSH) levels were lower in the brain in the winter and spring. Muscle tissue showed lower NPSH in the winter (site 1). Liver NPSH showed increased levels in liver in the spring and winter (site 2). The biochemical results clearly is related to pesticides and/or to the presence of other contaminants in the water such as metals or domestic sewage. The accumulation of pesticides in fish muscle added evidence that pesticides have been used in the area surrounding the Uruguay River. In conclusion, the biomarkers assayed in the present study could be used in future investigations considering other sampling sites along Uruguay River.
Subject(s)
Characidae/physiology , Environmental Monitoring , Pesticides/toxicity , Water Pollutants, Chemical/toxicity , Animals , Biomarkers/metabolism , Brazil , Characidae/metabolism , Characiformes/metabolism , Characiformes/physiology , Ecosystem , Gills/metabolism , Lipid Peroxidation , Metals/metabolism , Pesticides/analysis , Rivers/chemistry , Seasons , Water Pollutants, Chemical/analysisABSTRACT
Biofilms are a consortium of communities of organisms that live in syntrophic relationships and present a higher organization level than that of individual cells. Biofilms dominate microbial life in streams and rivers, enable crucial ecosystem processes, contribute to global biogeochemical flows and represent the main active bacterial life form. Epilithic biofilms are the main biomass found in rivers; their exposure to contaminants can lead to changes in their structure and composition. The composition of these communities is influenced by physicochemical factors, temperature, light and prior exposure to pollutants, among other factors, and it can be used for water quality monitoring purposes. The heterogenous composition of biofilms enables them to accumulate compounds in an integrative manner. Moreover, the availability of several sorption sites and their likely saturation can contribute to bioaccumulation. In aquatic environments, biofilms are also susceptible to the acquisition of antibiotic resistance genes and participate in their dissemination. Anthropic pressure intensification processes continuously expose water resources and, consequently, biofilm communities to different contamination sources. Therefore, the use of biofilms to indicate environmental pollution is reinforced by the progress of studies on the subject. Biofilm communities' response to pollutants in aquatic environments can be mainly influenced by the presence of different organisms, which may change due to community development or age. The current research aims to review studies about biofilm contamination and highlight the importance of biofilm use to better evaluate and maintain the quality of water bodies.
Subject(s)
Biofilms , Pesticides/metabolism , Pharmaceutical Preparations/metabolism , Water Pollutants, Chemical/metabolism , Biodegradation, Environmental , EcosystemABSTRACT
Sewage effluent effects on the biochemical parameters of Astyanax bimaculatus organs were investigateted. Treated sewage was collected in a treatment plant; 43 compounds, among them, pharmaceuticals and hormones, were investigated. Caffeine, ciprofloxacin, clindamycin, ofloxacin, oxytetracycline, paracetamol, sulfadiazine, sulfamethoxazole, sulfathiazole and tylosin waste was detected in the collected material. Fish were divided into four groups: control, TSE (treated sewage effluent), TSE + P (TSE with increased concentration of five pharmaceuticals) and PTSE (TSE + P post-treated with O3/H2O2/UV). Biochemical parameters were evaluated in different organs after 14-day exposure. TBARS levels increased significantly in the brain of animals in the TSE and TSE + P groups in comparison to the control. There was significant reduction in TBARS levels recorded for the liver, muscle and gills of animals in the PTSE group in comparison to those of animals in the other groups. AChE activity reduced in the muscle of animals in the groups showing the highest pharmaceutical concentrations. CAT activity in the liver of animals in groups exposed to pharmaceutical effluent was inhibited. GST activity increased in brain of animals in the TSE + P and PTSE groups, whereas reduced levels of this activity were observed in liver of animals in the TSE group. Increased GST activity was observed in the brain of animals in TSE + P and PTSE groups. Based on integrated biomarker response values, the TSE + P group presented greater changes in the analyzed parameters. Results point out that pharmaceutical waste can cause oxidative stress, as well as affect biochemical and enzymatic parameters in Astyanax sp. Post-treatment can also reduce damages caused to fish, even in case of the likely formation of metabolites. Based on these results, these metabolites can be less toxic than the original compounds; however, they were not able to fully degrade the pharmaceutical waste found in the sewage, which can interfere in fish metabolism.
Subject(s)
Environmental Monitoring , Pharmaceutical Preparations , Waste Disposal, Fluid , Water Pollutants, Chemical/toxicity , Animals , Fishes , Gills , Liver , SewageABSTRACT
Reservoirs are lentic man-made waterbodies resulting from river damming processes. Pollutants coming from adjacent areas can accumulate in the water and sediment of these modified freshwater environments. Fish are often found in reservoirs occupying several trophic niches. Biochemical biomarkers are early warning signals of environmental disturbance to an organism. It is essential to understand how pollutants, abiotic variables and biochemical biomarker responses behave throughout the seasons to implement biomonitoring programs. Loricariichthys anus and Geophagus brasiliensis were collected, and abiotic variables were seasonally measured for one year, at six sampling sites in Passo Real reservoir, in a subtropical region of Southern Brazil. Biochemical biomarkers were analyzed in four tissues of both fish species, as well as metal and pesticide concentrations in the reservoir's water and sediment. Redundancy analysis (RDA) was carried out to find the temporal relationship between biomarkers and environmental variables. RDA has clearly shown the separation of seasons for both species. Azoxystrobin, simazine and propoxur were the pesticides mostly contributing to the variation, whereas metals had lesser contribution to it. Seasonality appears to be the main factor explaining biomarkers' variability. PERMANOVA has confirmed the effect of temperature and dissolved oxygen on biomarkers of both fish species. Thus, it is hard to differentiate if the fluctuation in biomarkers' responses only reflects the normal state of organisms or it is a biological consequence from negative effects of fish exposure to several types of pollution (sewage, pesticides, and fertilizers) entering this aquatic system. In this study, to circumvent the seasonality issue on biomonitoring, the analysis of biomarkers on these fish should not be carried out in organs directly affected by temperature (such as liver and gills), or during reproduction periods (mainly in Spring).
Subject(s)
Environmental Monitoring , Water Pollutants, Chemical/analysis , Animals , Biomarkers , Brazil , SeasonsABSTRACT
World food production is directly related to human population growth. Chemicals are constantly applied to pest control in crops to increase productivity. Therefore, sustainable alternatives are needed to reduce environmental impacts. The biochemical responses in liver and muscle of Astyanax lacustris collected in different rice planting systems were analysed. Ten fish were collected in organic rice cultivation systems and conventional as well as water and sediment for pesticide analysis. In water from conventional system, bentazon (56.1 µg L-1), fipronil (0.226 µg L-1) and propoxur (0.141 µg L-1) were found, while azoxystrobin and quinclorac were below the limit of quantification (LOQ). There were no pesticides in the sediment from the conventional system. In the water of the organic system, only propoxur (below the LOQ) was registered. Metalaxil (0.025 µg kg-1) were verified in the sediment, while diphenoconazole, dinoxifene and tebuconazole were below the LOQ. The presence of these pesticides in crops with an organic production system may be related to proximity to crops to conventional production systems. Besides this, the amount is very low as compared with conventional system. Muscle glycogen, protein and amino acid levels were higher in fish collected in organic ponds. Lactate and ammonia levels were higher in conventional cultures. Lipids and proteins had greater oxidative damage in both tissues in the conventional system. Although pesticides were detected in organic sediments, the parameters of metabolic and oxidative damage were probably related by exposure to higher concentrations of pesticide in the water or by the lower oxygen content of conventional systems. However, fish mortality was not observed during collections. Despite being an anthropic environment, areas of organic cultivation seem to present better conditions for the survival of A. lacustris.
Subject(s)
Oryza , Pesticides/analysis , Water Pollutants, Chemical/analysis , Agriculture , Animals , Fishes , Humans , Organic AgricultureABSTRACT
Due to high global demand, large amounts of abbattoir waste are generated from pork production. Mismanagement of abattoir waste on agricultural lands can result in soil and water contamination with pathogens and contaminants like metals and nutrients. Therefore, possible effects on soil organisms prior to application should be evaluated. Thus, the aim of this study was to determine the effects of fresh pig abattoir waste (PAWf) and waste after stabilization processes on E. andrei through tests of avoidance behavior, acute toxicity and chronic toxicity. In order to do this, the waste was evaluated fresh (i.e., non-treated), and after aerated composting (PAWa), natural composting (PAWn) and vermicomposting (PAWv). In addition, we used a natural soil with no history of agricultural use as control soil. The evaluation was based on avoidance behavior, mortality, initial and final earthworm weight, and reproduction, in addition to a set of enzyme assays formed by acetylcholinesterase, lipid peroxidation, catalase and glutathione S-transferase measured over time. The ecotoxicological results showed that PAWf and PAWa increased AChE activity at different experimental periods, while PAWn decreased activity at 14 days compared to the control. PAWf and PAWa increased TBARS levels at 7 and 14 days, respectively. CAT activity decreased at 3, 7 and 14 days in PAWv, while GST activity increased at 3 days in PAWa and at 3 and 14 days in PAWf compared to the control. In the acute toxicity test, PAWa and PAWn had a toxic effect on E. andrei, resulting in 100% mortality at 14 days of exposure. Based on our findings, pig abattoir waste should undergo vermicomposting prior to agricultural application to soils.
Subject(s)
Industrial Waste , Oligochaeta , Soil Pollutants , Abattoirs , Animals , Enzyme Assays , Industrial Waste/analysis , Oligochaeta/drug effects , Soil/chemistry , Soil Pollutants/toxicity , SwineABSTRACT
Nowadays, many irrigated rice producers have adopted the rice-fish farming systems, in which fish are reared in the same environment as rice is grown. These animals can biologically control many pests that disturb the crop, as well as increase the income of producers when they are reared in the same area as rice. However, a large variety of pesticides is often used for pest control in conventional irrigated rice crops, and fish are exposed to different pesticide concentrations and active ingredients. Thus, the aim of the current study is to assess the potential risks of these pesticides to freshwater fish (Cyprinus carpio) cultured in integrated rice-fish farming systems. We assessed five pesticides widely used in rice culture. This is the first report on the bioaccumulation of the insecticides lambda-cyhalothrinâ¯+â¯thiamethoxam and clorantraniliprole and of the fungicide tebuconazoleâ¯+â¯trifloxystrobin associated with oxidative stress and with enzymatic and non-enzymatic parameters in edible fish samples reared in integrated in rice-fish farming systems. After 100â¯days of exposure, lambda-cyhalothrin and tebuconazole bioaccumulated in carp muscles. Acethylcholinesterase activity in the brain was lower in the experimental group than in the control group. Overall, the biochemical analyses showed increased responses in the experimental groups in comparison to the controls. Lipid peroxidation levels increased in the liver, gills and muscles. Protein oxidation increased in the liver and muscles. Catalase activity increased in the liver, and glutathione S-transferase increased in the liver, gills and muscles. Results demonstrate that these pesticides have severe adverse consequences in fish and indicate their potential risk to human health due to their bioaccumulation in farmed fish.
Subject(s)
Carps/metabolism , Oxidative Stress , Pesticides/toxicity , Water Pollutants, Chemical/toxicity , Animals , OryzaABSTRACT
Among all organotin compounds , triphenyltin hydroxide (TPhTH) is widely used as fungicide and moluscicide in Brazil. However, the effects of TPhTH on the biochemical parameters of non-target organisms, such as fish, are little known. The aim of the present study is to assess the possible toxic effects of different concentrations of waterborne TPhTH on silver catfish belonging to species Rhamdia quelen. The fish were exposed to two different concentrations of TPhTH (1.08 and 1.70 µg/L as Sn) for 15 days and then compared to the control group (triplicate, n = 3). The antioxidant profile (catalase (CAT) and the glutathione S-transferase (GST)) and the oxidative stress parameters (TBARS-thiobarbituric acid-reactive substances and protein carbonyl (PC)) were set after the exposure to TPhTH. The TBARS level and the PC content increased in several organs of the Rhamdia quelen (brain, liver, muscle and gills) under the two concentrations of TPhTH in comparison to the control group. The CAT activity in the liver and gills has enhanced in all tested TPhTH concentrations. The GST activity increased in the brain, liver and muscle tissues under all the TPhTH concentrations. The significant changes in the biomarkers indicated that the investigated pesticide could have harmful effect on fish, in the field. However, these biomarkers were measured after the fish received doses lower than the recommended for use in agriculture.
Subject(s)
Catfishes/metabolism , Organotin Compounds/toxicity , Oxidative Stress/physiology , Water Pollutants, Chemical/toxicity , Animals , Antioxidants/metabolism , Biomarkers/metabolism , Brazil , Catalase/metabolism , Fish Proteins/metabolism , Gills/metabolism , Glutathione Transferase/metabolism , Liver/drug effects , Oxidation-Reduction , Pesticides/metabolism , Superoxide Dismutase/metabolism , Thiobarbituric Acid Reactive Substances/metabolismABSTRACT
Agricultural practices are directly related to the use of pesticides, which indiscriminately and without due care may contribute to the occurrence of numerous intoxications. Several studies have demonstrated the relationship of certain pesticides and the occurrence of oxidative stress and therefore, in recent years have developed methods of analysis of several biomarkers of cellular damage that can be measured and quantified. In this context, the objective of this study was to evaluate the possible changes in biochemical markers: glutamic aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, urea, total protein, and oxidative markers such as lipid peroxidation, damage to proteins and the activity of the enzymes acetylcholinesterase (AChE) and catalase (CAT) in farmers exposed to different pesticides for at least five years from Ibirubá - RS city. With the exception of AST and ALT, the results showed a significant difference between the mean total protein, urea and creatinine in the control group, showing that no changes in liver or kidney function of rural workers. In the oxidative parameters, there was a decrease in AChE activity and CAT in the control group; there were an increase in protein carbonyl and a decreased on TBARS compared to control group. Therefore, the results demonstrated a change in oxidative status of rural workers compared with the control group, mainly by possible inhibition of AChE activity and the occurrence of oxidative stress without showing changes in biochemical parameters.
As práticas agrícolas estão diretamente relacionadas com o uso de agrotóxico, que, de forma indiscriminada e sem o devido cuidado, podem contribuir para a ocorrência de inúmeras intoxicações. Diversos estudos demonstraram a relação de determinados pesticidas e a ocorrência do estresse oxidativo e, portanto, nos anos recentes, tem-se desenvolvido métodos de análise de diversos biomarcadores de dano celular, o qual pode ser medido e quantificado. Nesse contexto, o objetivo deste estudo foi avaliar as possíveis alterações nos marcadores bioquímicos: aspartato aminotransferase (AST), alanina aminotransferase (ALT), creatinina, ureia, proteína total, e marcadores oxidativos como: a peroxidação de lipídios, danos nas proteínas e a atividade das enzimas acetilcolinesterase (AChE) e catalase (CAT), em agricultores da cidade de Ibirubá, RS, expostos a diferentes agrotóxicos por pelo menos cinco anos. Com exceção da AST e da ALT, os resultados mostraram diferença significativa entre as médias das proteínas totais, ureia e creatinina, no grupo controle, mostrando que não houve alterações na função hepática ou renal dos trabalhadores rurais. Nos parâmetros oxidativos avaliados, houve uma diminuição da atividade da AChE e da CAT em relação ao grupo controle; um aumento das proteínas carboniladas e uma diminuição dos níveis de TBARS em relação ao grupo controle. Portanto, os resultados demonstram uma alteração oxidativa nos trabalhadores rurais, comparados com o grupo controle, principalmente pela possível inibição da AChE e ocorrência do estresse oxidativo, sem demonstrar mudança nos parâmetros bioquímicos analisados.
ABSTRACT
Effects of carbofuran commercial formulation on oxidative stress parameters were studied in carps (Cyprinus carpio) exposed to 50µg/L for 7 and 30 days under rice field and laboratory conditions. Thiobarbituric acid reactive substance (TBARS) levels were increased in the brain of fish after 7 and 30 days under rice field and laboratory conditions. In the liver and muscle, TBARS levels increased after 7 and 30 days under laboratory conditions, whereas in rice field the levels increased only after 30 days. Protein carbonyl content in the liver increased after 7 and 30 days under both experimental conditions. Acetylcholinesterase (AChE) activity was decreased in the brain and muscle after 7 and 30 days under both experimental conditions evaluated. The superoxide dismutase (SOD) activity increased in the liver after 7 and 30 days under rice field condition, whereas under laboratory condition this enzyme increased only after 30 days. The catalase (CAT) activity in the liver decreased after 30 days under rice field condition, whereas no changes were observed under laboratory conditions. In rice field, glutathione S-transferase (GST) decreased after 7 days but increased after 30 days, whereas no change was observed in fish exposed to carbofuran under laboratory conditions. These results suggest that environmental relevant carbofuran concentrations may cause oxidative stress, affecting biochemical and enzymatic parameters on carps. Some parameters could be used as biomarkers to carbofuran exposure.
Subject(s)
Carbofuran/toxicity , Carps/physiology , Oxidative Stress/drug effects , Water Pollutants, Chemical/toxicity , Animals , Brain/drug effects , Carbofuran/analysis , Enzyme Activation/drug effects , Enzymes/metabolism , Fresh Water/chemistry , Liver/drug effects , Liver/enzymology , Muscles/drug effects , Oryza , Thiobarbituric Acid Reactive Substances/metabolism , Water Pollutants, Chemical/analysisABSTRACT
Toxic effects of penoxsulam herbicide on acetylcholinesterase, thiobarbituric acid-reactive substances and protein carbonyl were studied in silver catfish (Rhamdia sp.) and carp (Cyprinus carpio). Acetylcholinesterase activity was inhibited in both brain and muscle tissue, with the inhibition being greater in carp than in silver catfish. The levels of malondialdehyde (MDA), an indicator of lipid peroxidation, decreased in silver catfish brain tissue, but increased in the carp brain. MDA also increased significantly in muscle tissue of silver catfish. The levels of protein carbonyl, another measure of oxidative damage, increased in the brain of both fish species, and in the muscle of carp. However, silver catfish exhibited a decrease in muscle protein carbonyl. It appears that silver catfish may possess better mechanisms of defense against penoxsulam toxicity than carp.