Expression-based, consistent biomarkers for prognosis and diagnosis in lung cancer.

OVERVIEW: Lung cancer is one of the deadliest cancers in the world. Its histological classification depends on early diagnosis and successful treatment. Therefore, having specific biomarkers for a quick sorting widens the successful output of lung cancer treatment. MATERIAL AND METHODS: High-throughput sequencing (RNA-seq) was performed of small cohorts of BioBanco samples from healthy and tumour cells from lung adenocarcinoma (LUAD) and squamous cell carcinoma of the lung (lSCC). RNA-seq samples from small cell lung cancer (SCLC) were downloaded from databases. A bioinformatic workflow has been programmed for the identification of differentially expressed genes (DEGs). RESULTS: A total of 4777 DEGs were differentially expressed in SCLC, 3676 DEGs were in lSCC, while the lowest number of DEGs, 2819, appeared in LUAD. Among them, 945 DEGs were common to the three histological types. Once validated their expression profile and their survival predictive capacity in large, public cohorts, three DEGs can be exclusively considered as diagnostic biomarkers, three as prognosis biomarkers, and other three exhibit both diagnosis and prognosis capabilities. CONCLUSIONS: This prospective study presents evidences for the diagnostic and prognostic capabilities of expression changes in CAPN8-2, TMC5 and MUC1 in LUAD, while they are non-significant in SCLC and lSCC. Their translation to clinical practice is proposed.

SEOM clinical guidelines for the treatment of small-cell lung cancer (SCLC) (2019).

Small-cell lung cancer (SCLC) accounts for 15% of lung cancers. Only one-third of patients are diagnosed at limited stage. The median survival remains to be around 15-20 months without significative changes in the strategies of treatment for many years. In stage I and IIA, the standard treatment is the surgery followed by adjuvant therapy with platinum-etoposide. In stage IIB-IIIC, the recommended treatment is early concurrent chemotherapy with platinum-etoposide plus thoracic radiotherapy followed by prophylactic cranial irradiation in patients without progression. However, in the extensive stage, significant advances have been observed adding immunotherapy to platinum-etoposide chemotherapy to obtain a significant increase in overall survival, constituting the new recommended standard of care. In the second-line treatment, topotecan remains as the standard treatment. Reinduction with platinum-etoposide is the recommended regimen in patients with sensitive relapse (≥ 3 months) and new drugs such as lurbinectedin and immunotherapy are new treatment options. New biomarkers and new clinical trials designed according to the new classification of SCLC subtypes defined by distinct gene expression profiles are necessary.

Factores asociados al ataque cerebrovascular isquémico entre los años 2013 a 2016, un estudio de casos y controles / Factors associated with ischaemic cerebrovascular accidents between the years 2013 and 2016: A case-control study

Resumen Objetivo: Determinar los factores asociados al ataque cerebrovascular isquémico en el servicio de urgencias de la Clínica Especializada Los Andes, de la ciudad de Tunja, entre los años 2013 a 2016. Pacientes y métodos: Estudio de casos y controles; los casos correspondieron a 97 pacientes con ataque cerebrovascular isquémico (infarto cerebral isquémico y accidente isquémico transitorio) y los controles a 291 pacientes sin ataque cerebrovascular isquémico que ingresaron a urgencias entre los años 2013 a 2016. Resultados: El sexo femenino correspondió al 56,7% (55) de los casos y al 54,6% de los controles (154) (p = 0,069). La media de edad en el grupo caso fue de 73,7 años ENT#091;DE: 10,5 añosENT#093; y en los controles de 64,5 años ENT#091;DE: 11,3 añosENT#093;. Los factores asociados al ataque cerebrovascular isquémico fueron: antecedente de ataque cerebrovascular isquémico ENT#091;OR 7,7 IC 95% 3,2; 18 p= 0,000ENT#093;, tabaquismo ENT#091;OR 4,4 IC 95% 1,1; 18 p= 0,022ENT#093;, dislipidemia ENT#091;OR 3 IC 95% 1,2; 7,5 p= 0,017ENT#093;, edad igual o mayor a 70 años ENT#091;OR 2,3 IC 95% 1,3; 4,1 p= 0,002ENT#093; e hipertensión arterial ENT#091;OR 1,8 IC 95% 1,06; 3,3 p= 0,029ENT#093;. Conclusiones: Los factores asociados al ataque cerebrovascular isquémico fueron, en orden de importancia, antecedente de ataque cerebrovascular isquémico, tabaquismo, dislipidemia, edad igual o mayor a 70 años E hipertensión arterial.

Abstract Objective: To determine the factors associated with ischaemic cerebrovascular accidents (ICVA) in the Emergency Department of the Andes Specialist Clinic of the city of Tunja, between the years 2013 and 2016. Patients and methods: A case-control study was conducted in which the cases consisted of 97 patients with ICVA (ischaemic cerebral infarction and transient ischaemic accident), and the controls were 291 patients with no ICVA, who were admitted to the Emergency Department between the years 2013 and 2016. Results: There were 56.7% (55) females in the cases, and 54.6% (154) in the controls (P=.069). The mean age of the cases was 73.7 years ENT#091;SD: 10.5 yearsENT#093;, and 64.5 years ENT#091;SD: 11.3 yearsENT#093; in the controls. The factors associated with ICVA were: a history of ICVA ENT#091;OR; 7.7, 95% CI; 3.2-18, P=.000ENT#093;, smoking ENT#091;OR; 4.4, 95% CI; 1.1-18, P=.022ENT#093;, dyslipidaemia ENT#091;OR; 3, 95% CI; 1.2-7.5, P=.017ENT#093;, age equal to or greater than 70 years ENT#091;OR; 2.3, 95% CI; 1.3-4.1, P=.002ENT#093;, and arterial hypertension ENT#091;OR; 1.8, 95% CI; 1.06-3.3, P=.029ENT#093;. Conclusions: The factors associated with ischaemic cerebrovascular accident were, in order of importance, a history of ischaemic cerebrovascular accident, smoking, dyslipidaemia, age equal to or greater than 70 years, and arterial hypertension.

A consensus statement on the gender perspective in lung cancer.

Lung cancer is the most common cancer globally and has the highest mortality. Although this disease is not associated with a particular gender, its incidence is rising among women, who are diagnosed at an increasingly younger age compared with men. One of the main reasons for this rise is women taking up smoking. However, many non-smoking women also develop this disease. Other risk factors implicated in the differential development of lung cancer in women are genetic predisposition, tumour histology and molecular profile. Proportionally more women than men with lung cancer have a mutation in the EGFR gene. This consensus statement reviews the available evidence about the epidemiological, biological, diagnostic, therapeutic, social and psychological aspects of lung cancer in women.

Long-term survival in advanced non-squamous NSCLC patients treated with first-line bevacizumab-based therapy.

BACKGROUND/AIM: First-line bevacizumab-based therapies have been shown to improve clinical outcomes in patients with non-squamous non-small-cell lung cancer (NSCLC). We aimed to descriptively analyse patients with non-squamous NSCLC who received a long-term period of maintenance bevacizumab. PATIENTS AND METHODS: This retrospective study included 104 patients who had already reached a progression-free survival (PFS) of at least 9 months. RESULTS: Median overall survival and PFS were 30.7 and 15.1 months, respectively. The overall response rate was 83 %. Weight loss ≤5 %, ECOG PS = 0, or low number of metastatic sites seem to be predictive factors of good evolution. The incidence of bevacizumab-related adverse events appeared to be similar as the previous studies. CONCLUSION: Our findings show that there is a long-term survivor group whom the administration of bevacizumab resulted in a relevant prolongation of response without new safety signals. Due to the population heterogeneity, it was not possible to identify the standardised predictive factors.

Environmental variation of PCDD/Fs and dl-PCBs in two tropical Andean Colombian cities using passive samplers.

Passive air-sampling data of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and dioxin-like polychlorinated biphenyls (dl-PCBs) taken in Manizales (a medium-sized city) and Bogotá (a megacity), Colombia, were analyzed in order to identify potential sources of pollution and the possible influence of meteorological variables like temperature and precipitation. The results indicate important differences in levels of PCDD/Fs and dl-PCBs between Bogotá and Manizales, attributed to differences in site characteristics and potential local/regional sources. Higher PCDD/Fs concentrations were observed in Bogotá (373fg/m(3)) compared to those observed in Manizales, with mean levels ranging from 64fg/m(3) in a residential zone to 151fg/m(3) around a vehicular-influenced area. Higher dl-PCBs concentrations were observed in the industrial area of Manizales compared to those observed in Bogotá, with mean levels of 6668fg/m(3) and 4388fg/m(3) respectively. In terms of PCDD/Fs congener distribution, there was a predominance of octachlorodibenzodioxin (OCDD) followed by 1,2,3,4,6,7.8-heptachlorodibenzofuran (HpCDF) congeners, with both cities showing higher levels in zones of high vehicular activity. Industrial influence was most evident in dl-PCB levels. In comparison to the mean levels of dl-PCB congeners obtained in the vehicular zones of Bogotá and Manizales, the industrially influenced sampling stations showed higher concentrations of dl-PCB congeners. Passive sampling results suggested that congener concentration profiles are characteristic of their different emission sources, and can be used to distinguish between their industrial or vehicular origins.

SEOM clinical guidelines for the treatment of non-small cell lung cancer (NSCLC) 2015.

Lung cancer is the most common cancer worldwide as well as the leading cause of cancer related deaths as reported by Torre et al (CA Cancer J Clin 65:87-108, 2015]. Non-small cell lung cancer (NSCLC) accounts for up to 85 % of all lung cancers. Multiple advances in the staging, diagnostic procedures, therapeutic options, as well as molecular knowledge have been achieved during the past years, although the overall outlook has not greatly changed for the majority of patients with the overall 5-year survival having marginally increased over the last decade from 15.7 to 17.4 % as reported by Howlader et al. (SEER Cancer Statistics Review 2015).

Recommendations for radiological diagnosis and assessment of treatment response in lung cancer: a national consensus statement by the Spanish Society of Medical Radiology and the Spanish Society of Medical Oncology.

The last decade has seen substantial progress in the diagnostic and therapeutic approach to lung cancer, thus meaning that its prognosis has improved. The Spanish Society of Medical Radiology and the Spanish Society of Medical Oncology have therefore produced a national consensus statement to make recommendations for radiological diagnosis and assessment of treatment response in patients with lung cancer. This expert group recommends multi-detector computed tomography as the technique of choice for investigating this disease. The radiology report should include a full assessment by the TNM staging system. Lastly, when the patient is on immunotherapy, response evaluation should employ not only response evaluation criteria in solid tumours, but also immune-related response criteria.

Baseline levels of dioxin and furan emissions from waste thermal treatment in Colombia.

Background data of polychlorinated dibenzodioxin and dibenzofuran (PCDD/Fs) emissions from the incineration sector in Colombia are presented. Monitoring was carried out during a two-year period, 2003-2005. Twelve plants were sampled for stack gas emissions of dioxins and total solid particulate (TSP). Additionally, PCDD/Fs in several fly ash samples were analyzed. Most incinerators burned industrial refuse materials and medical residues. A wide range of PCDD/Fs emission levels were found. In particular, levels ranging from 6.9 to 343.8 ng I-TEQ/N m(3) were determined in plants without any air pollution control system (APCS). In contrast, 0.5-39.2 ng I-TEQ/N m(3) levels were found in plants with APCS while 8.5-67.5 ng I-TEQ/g were measured in fly ash samples. TSP values ranged from 14 to 448 mg/N m(3). This study also evaluated the impact of implementing different control systems in an incinerator. Finally, for comparison purposes several samples were analyzed by both high resolution gas chromatography coupled to high resolution mass spectrometry (HRGC-HRMS) and high resolution gas chromatography coupled to ion-trap low resolution mass spectrometry-mass spectrometry (HRGC-IT LRMS/MS). Overall, I-TEQ values deviated about 20-30% between both techniques.

Total suspended particulate (TSP), polychlorinated dibenzodioxin (PCDD) and polychlorinated dibenzofuran (PCDF) emissions from medical waste incinerators in Antioquia, Colombia.

Results of a preliminary survey of particulate and dioxin emissions in combustion gases from hospital waste incinerators in Antioquia-Colombia are presented. Base line data of total suspended particulate (TSP) and polychlorinated dibenzodioxin and dibenzofuran (PCDD/PCDF) emissions in incinerators from health care institutions in Antioquia-Colombia will be used to improve the management of medical waste in the local context. All monitored incinerators are batch operated. TSP exceeds 80 mg Nm(-3) in 8 out of 12 incinerators. Dioxin emissions are in the range from about 7 to 700 I-TEQ (ng Nm(-3)). Such a significant amount of dioxin emissions did correlate with entrained particulate matter, mainly as a consequence of poor control of operation parameters. Several suggestions are made to improve medical waste management practices in Colombia.

Glucagonoma with two pancreatic masses and pulmonary metastases as debut of MEN-1.

This is a rare case of a patient with type 1 multiple endocrine neoplasia (MEN-1) syndrome. The case is further unusual in that the glucagonoma debuted with two synchronic pancreatic masses at the time of diagnosis and with pulmonary metastases as the primary site of metastasis and not the more usual site of the liver.

Dioxin emissions from thermal waste management in Medellín, Colombia: present regulation status and preliminary results.

Preliminary results of a study undertaken to characterize dioxin and furan releases from waste incineration plants operating in Medellín-Colombia are presented. Emission and fly ash samples were collected from representative plants burning medical and industrial residues to characterize PCDD/PCDF levels. Analyses were carried out following European standards for stationary gas emissions EN-1948:1996 and US EPA 1613 (fly ashes). Final extracts were analyzed by both high resolution gas chromatography coupled to high resolution mass spectrometry (HRGC-HRMS) and high resolution gas chromatography coupled to ion trap tandem mass spectrometry (HRGC-ITMS/MS). Preliminary results revealed emission levels of 1-30.3 ng I-TEQ/Nm3 depending on whether or not an air pollution control system (APCS) was installed. Fly ashes contained 8.5-68 ng I-TEQ/g. Critical issues that should be addressed in an assessment and in developing waste management plans in Colombia are discussed.

Conservative treatment with transurethral resection, neoadjuvant chemotherapy followed by radiochemotherapy in stage T2-3 transitional bladder cancer.

PURPOSE: Organ preservation has been investigated in patients (p) with infiltrating transitional cell carcinoma (TCC) of the bladder over the past decade as an alternative to radical cystectomy. This is a trimodal schedule study, including transurethral resection of bladder tumor (TURB), neoadjuvant chemotherapy and concomitant radiochemotherapy (RTC). PATIENTS AND METHODS: From April 1996 until August 2005, 29 evaluable patients (p) with T2-T3NXM0 bladder cancer were enrolled. After a transurethral resection of bladder tumor (TURB), we administered 2 cycles of induction chemotherapy with CMV (15 p) or Gemcitabine-Cisplatin (14 p) followed by radiotherapy 45 Gy 1.8 Gy/fraction and two cycles of concomitant cisplatin 70 mg/m(2). 2-3 weeks later, a cystoscopy with tumor-site biopsy was performed. If complete histological response, p were treated with consolidation radiotherapy until 64.8 Gy. For p with residual or recurrent tumor, cystectomy was performed. RESULTS: We included 28 men and 1 women (median age 63, range 39-72 years) with PS (ECOG) 0-1. The stage was: 21 p T2; 6 p T3a; and 2 p T3b. Toxicity was higher in CMV compared with Gem- Cis: grade (3/4) neutropenia 4/15 (26%) vs 1/14 (7%); febrile neutropenia 3/15 (20%) vs 1/14 (7%); grade (3/4) trombocytopenia 2/15 (13%) vs 1/14 (7%). Toxicities with concomitant RCT were low-moderate: urocystitis (26%) and enteritis (18%). RESPONSE: microscopically complete TURB was obtained in 20 p (69%), but not in 9 p (31%) (7 microscopic, and 2 macroscopic residual tumor). We found a complete histologic response after induction RCT in 25 p (86%). After a median follow-up of 69.4 months (m) (range: 8-97.7), there were 8 deaths, with a overall survival of 72%. Furthermore 14 of 29 p (48%) were alive with intact bladder, and median survival time with intact bladder was 63.6 m (50.1-77.2); were predictive of best outcome T2 stage vs T3 (p < 0.0001), and complete histologic resection in initial TURB vs residual tumor (p = 0.0004). CONCLUSIONS: Combined treatment provide high response rates and can be offered as an alternative option to radical cystectomy in selected patients with TCC. Patients with T2 stage and complete histologic resection in initial TURB had the best outcome.

Reconstructing population history using JC virus: Amerinds, Spanish, and Africans in the ancestry of modern Puerto Ricans.

The roots of the Hispanic populations of the Caribbean Islands and Central and South America go back to three continents of the Old World. In Puerto Rico major genetic contributions have come from (1) Asians in the form of the aboriginal Taino population, an Arawak tribe, present when Columbus arrived on the Island, (2) Europeans, largely Spanish explorers, settlers, government administrators, and soldiers, and (3) Africans who came as part of the slave trade. Since JC virus (JCV) genotypes characteristic of Asia, Europe, and Africa have been identified, and excretion of JCV in urine has been proposed as a marker for human migrations, we sought to characterize the JCV strains present in a Caribbean Hispanic population. We found that the strains of JCV present today in Puerto Rico are those derived from the Old World populations represented there: Types 1B and 4 from Spain, Types 3A, 3B, and 6 from Africa, and Type 2A from Asia. The Type 2A genotype represents the indigenous Taino people. This JCV genotype was represented much more frequently (61%) than would be predicted by the trihybrid model of genetic admixture. This might be attributable to characteristics of JCV Type 2A itself, as well as to the nature of the early relationships between Spanish men and native women. These findings indicate that the JCV strains carried by the Taino Indians can be found in today's Puerto Rican population despite the apparent demise of these people more than two centuries ago. Therefore, molecular characterization of JCV provides a tool to supplement genetic techniques for reconstructing population histories including admixed populations.

Characterization of an outbreak of tetM-containing Neisseria gonorrhoeae in Argentina.

Phenotypic and molecular characterization of an outbreak of 9 Neisseria gonorrhoeae (NG) isolates exhibiting high-level plasmid mediated resistance to penicillin and tetracycline (PP-TRNG) that took place in Tandil, Argentina between February and April 1995. Comparison with the patterns of the 3 PP-TRNG strains previously isolated were made. We determined the following markers for each strain: antimicrobial susceptibility, serogroup, auxotype, plasmid profile, presence of tetM determinant and restriction pattern of the tetM-containing plasmid. Antimicrobial tests values were: tetracycline disk diameter 12-14 mm, minimum inhibitory concentration (MIC) 32 micrograms/ml; penicillin disk diameter 6 mm, MIC 32 micrograms/ml and sensitive by both methods to spectinomycin, cefuroxime, ceftriaxone and ciprofloxacin. All isolates were of the same serogroup (WI). Ten of the strains, including the 9 from Tandil outbreak, were arginine-requiring, while the other 2 were methionine and arginine-requiring. All of them demonstrate the same plasmid profile (2.6, 3.2, 25.2 MDa). They were positive for the tetM determinant and the restriction analysis identified it is a Dutch-type plasmid. In spite of the temporal and geographical dispersion, PP-TRNG strains in Argentina seem to be highly homogeneous in terms of antimicrobial susceptibility, serogroup, plasmid profiles and even auxotype.

PIP: This study determines the phenotypic and molecular characterization of an outbreak of 9 Neisseria gonorrhea isolates exhibiting high-level plasmid-mediated resistance to penicillin and tetracycline (PP-TRNG) that took place in Tandil, Argentina. Subjects included patients attending Tandil's Sanatamarina Hospital. Individuals infected with sexually transmitted disease were examined using a standardized questionnaire. Results show that all isolates produced B-lactamase, were penicillin resistant (disk zone diameter 6 mm, MIC 32 mcg/ml) and high-level tetracycline resistant (disk zone diameter 12-14 mm, MIC 32 mcg/ml). Moreover, all strains were fully sensitive to cefuroxime, ceftriaxone, ciprofloxacin, and spectinomycin; while autotype and serogroup results showed that all the strains were arginine-requiring and of serogroup WI. In spite of the temporal and geographical dispersion, PP-TRNG strains in Argentina seem to be highly homogeneous in terms of antimicrobial susceptibility, serogroup, plasmid profiles, and even auxotype.

[Characterization of isolated adenovirus associated with acute lower respiratory infection in pediatrics]. / Caracterización de aislamientos de adenovirus asociados a infección respiratoria aguda baja en pediatría.

BACKGROUND: Acute respiratory infection (ARI) are a health care problem as the adenovirus (ADV) has shown to be one of the most frequent viral agents detected in children admitted for mild ARI in the authors medium. METHODS: Over a 7-year period (1988-1994) ADV isolated from patients under the age of 5, admitted for mild ARI in hospitals in the city of Buenos Aires (Argentina). All the strains were isolated in HEp-2 cell cultures from nasopharyngeal aspirates in which the presence of ADV was detected by indirect immunofluorescence with monoclonal antibodies. Antigenic characterization was performed by sero- and genome neutralization with restriction enzymes. RESULTS: The isolates corresponded to the genomic variants of ADV 7i, ADV 7c and to a greater number of ADV 7h. An increase was observed in the quantity of cases in the second half of the year. In the population studied, the most commonly infected were males (67.9%) and patients from 2 months to 1 year in age (89.2%). Sixty-six percent of the cases were severe infections with the length of hospitalization being greater than that of patients normally admitted for mild ARI by other virus and showed a high mortality. CONCLUSIONS: All the above events suggest that the genomic variants detected are highly pathogenic.

Primer informe en la República Argentina de resistencia a penicilina en Neisseria gonorrhoeae mediada por el plásmido de 3,2 MDal (africano) / First report in Argentine of resistance to penicillin in Neisseria gonorrhoeae madiated by the 3,2 MDal African plasmid

En la República Argentina, Buenos Aires (INM), detectamos la primer cepa productora de ß-lactamasa en 1980, incrementándose la prevalencia de estas cepas resistentes de 1,9 por ciento (1980-1984) a 30 por ciento (1984-1988) y a 37 por ciento (1989-1991). El objetivo de este trabajo es la caracterización de los plásmidos responsables de esta resistencia, en un estudio retrospectivo que abarca de 1985 a 1991. Se estudiaron 28 cepas de Neisseria gonorrhoeae productoras de penicilinasa (PPNG), enviadas para su estudio o aisladas en nuestro Servicio de exudados genitales. El 100 por ciento de las cepas presentó el plásmido críptico de 2,6 MDal. El plásmido de 3,2 MDal se encontró en 13 cepas (46,4 por ciento) y en el 92,3 por ciento de ellas acompañado por el de 24,4 MDal. En 15 cepas (53,60 por ciento) se detectó la presencia del plásmido de 4,4 MDal, sólo en 9 de ellas (60 por ciento) junto con el plásmido conjugativo. Este es el primer informe sobre la presencia del plásmido de 3,2 MDal (africano) en las cepas PPNG aisladas en la Argentina. Nuestro primer hallazgo de este plásmido data de 1988 (AU)

Primer informe en la República Argentina de resistencia a penicilina en Neisseria gonorrhoeae mediada por el plásmido de 3,2 MDal (africano) / First report in Argentine of resistance to penicillin in Neisseria gonorrhoeae madiated by the 3,2 MDal African plasmid

En la República Argentina, Buenos Aires (INM), detectamos la primer cepa productora de ß-lactamasa en 1980, incrementándose la prevalencia de estas cepas resistentes de 1,9 por ciento (1980-1984) a 30 por ciento (1984-1988) y a 37 por ciento (1989-1991). El objetivo de este trabajo es la caracterización de los plásmidos responsables de esta resistencia, en un estudio retrospectivo que abarca de 1985 a 1991. Se estudiaron 28 cepas de Neisseria gonorrhoeae productoras de penicilinasa (PPNG), enviadas para su estudio o aisladas en nuestro Servicio de exudados genitales. El 100 por ciento de las cepas presentó el plásmido críptico de 2,6 MDal. El plásmido de 3,2 MDal se encontró en 13 cepas (46,4 por ciento) y en el 92,3 por ciento de ellas acompañado por el de 24,4 MDal. En 15 cepas (53,60 por ciento) se detectó la presencia del plásmido de 4,4 MDal, sólo en 9 de ellas (60 por ciento) junto con el plásmido conjugativo. Este es el primer informe sobre la presencia del plásmido de 3,2 MDal (africano) en las cepas PPNG aisladas en la Argentina. Nuestro primer hallazgo de este plásmido data de 1988

[Detection of a new genomic variation of Adenovirus 7 associated to severe infections of the lower respiratory tract]. / Detección de una nueva variación genómica de Adenovirus 7 asociada a infecciones severas del tracto respiratorio inferior.

We report a new genomic variation of Adenovirus 7, associated to severe infections of the lower respiratory tract isolated during September 1990, from children under 3 years of age and living in Buenos Aires city. The restriction analysis with the BamHI, BglI, BglII and SmaI restriction endonucleases demonstrated that the new variation is highly related to the recently described Adenovirus 7h.

Detección de una nueva variación genómica de Adenovirus 7 asociada a infecciones severas del tracto respiratorio inferior. / [Detection of a new genomic variation of Adenovirus 7 associated to severe infections of the lower respiratory tract]

We report a new genomic variation of Adenovirus 7, associated to severe infections of the lower respiratory tract isolated during September 1990, from children under 3 years of age and living in Buenos Aires city. The restriction analysis with the BamHI, BglI, BglII and SmaI restriction endonucleases demonstrated that the new variation is highly related to the recently described Adenovirus 7h.