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1.
Bioresour Technol ; 128: 646-54, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23220111

ABSTRACT

A strain of Pseudomonas aeruginosa isolated from a site contaminated with refined oil products exhibited demulsification capabilities against Tween 80-Span 80 stabilized oil-in-water (O/W), Tween 80-stabilized water-in-oil (W/O) model emulsions (kerosene-water), and an industrial emulsion (Daido Dairoll PA-5A). GC-MS analysis confirmed the presence of fatty acids and carbohydrates in the extracellular biodemulsifier. The demulsifying activity of cells and culture supernatants was favored by growth in media containing 1% diesel oil. There was a correlation between culture age, de-emulsification and cellular hydrophobicity, and highest activities were observed for cells and supernatants from 96-h cultures. Activity increased with addition of up to 60 mg cells or 300 µL supernatant to emulsions. The activity was relatively stable at 20-40 °C and to freezing, but was reduced by 69% by washing the cells with chloroform-methanol-water. This demulsifier has potential for application in biotreatment of emulsified oily wastewaters to promote recovery and/or degradation of oil.


Subject(s)
Industrial Oils/microbiology , Pseudomonas aeruginosa/metabolism , Soil Microbiology , Soil Pollutants/chemistry , Soil Pollutants/isolation & purification , Biodegradation, Environmental , Emulsions/chemistry , Emulsions/metabolism , Pseudomonas aeruginosa/classification , Species Specificity
7.
Rev. Inst. Adolfo Lutz ; 1(2): e33073, fev.9, 1941. ilus
Article in Portuguese | LILACS, CONASS, Coleciona SUS, Sec. Est. Saúde SP, SESSP-IALPROD, Sec. Est. Saúde SP, SESSP-IALACERVO | ID: biblio-1381350

ABSTRACT

O autor. após tecer comentários sobre a falta de estudos sistematizados quanto à epidemiologia da Moléstia de Chagas no Estado de São Paulo, relata em nota os resultados obtidos na fase inicial de um inquérito realizado em uma localidade (Núcleo Colonial Barão de Antonina, município de Itaporanga, S. Paulo, Brasil). Tendo separado 50 pessoas, da localidade, moradores em casas com triatomas infectados, encontrou 4 com Trypanosoma cruzi no sangue periférico; o que dá uma porcentagem de 8 % de doentes. Como não houve escolha de indivíduos acha que tal índice é muito elevado. Ressalta o valor do Xenodiagnóstico como meio de diagnóstico para a doença, tanto na fase aguda como nos casos crônicos. Chama a atenção para a menor eficácia da gota espessa em relação ao último e descrê do valor da intradermorreação com antígeno de Trypanosoma cruzi. Tece comentários em torno do tipo de habitação existente na localidade. (AU)


Subject(s)
Information Storage and Retrieval , Chagas Disease
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