ABSTRACT
This article documents the addition of 142 microsatellite marker loci to the Molecular Ecology Resources database. Loci were developed for the following species: Agriophyllum squarrosum, Amazilia cyanocephala, Batillaria attramentaria, Fungal strain CTeY1 (Ascomycota), Gadopsis marmoratus, Juniperus phoenicea subsp. turbinata, Liriomyza sativae, Lupinus polyphyllus, Metschnikowia reukaufii, Puccinia striiformis and Xylocopa grisescens. These loci were cross-tested on the following species: Amazilia beryllina, Amazilia candida, Amazilia rutila, Amazilia tzacatl, Amazilia violiceps, Amazilia yucatanensis, Campylopterus curvipennis, Cynanthus sordidus, Hylocharis leucotis, Juniperus brevifolia, Juniperus cedrus, Juniperus osteosperma, Juniperus oxycedrus, Juniperus thurifera, Liriomyza bryoniae, Liriomyza chinensis, Liriomyza huidobrensis and Liriomyza trifolii.
Subject(s)
Computational Biology/methods , Genomics/methods , Microsatellite Repeats , Animals , Bees/genetics , Birds/genetics , Fishes/genetics , Fungi/genetics , Plants/geneticsABSTRACT
PREMISE OF THE STUDY: Eighteen microsatellite loci of distylous Palicourea padifolia were isolated and characterized for population genetics studies. METHODS AND RESULTS: Following a microsatellite enrichment protocol, 18 primer pairs amplified successfully, and the polymorphism of the loci was initially evaluated in 15 individuals from three populations in Mexico. Seven loci were polymorphic, and their variability was further assessed in 60 individuals from three populations. The total number of alleles per locus, combining samples from all populations, ranged from 7 to 12. Nei's genetic diversity average across loci was 0.681, 0.714, and 0.703 for El Riscal, Montebello, and Ruiz Cortínez, respectively. Palicourea padifolia individuals had one to four alleles, confirming the polyploidy of this species. CONCLUSIONS: These markers will facilitate genetic diversity studies of P. padifolia across its distribution range and facilitate investigations on the evolution of its breeding system.