ABSTRACT
CASE REPORT: This case report shows a compliance-free treatment of an anterior open bite in a 11-year-old girl. This treatment is also non invasive, therefore well accepted by the patient and her parents.
Subject(s)
Open Bite/therapy , Orthodontic Appliances , Orthodontics, Corrective/instrumentation , Cephalometry , Child , Female , Humans , Open Bite/diagnostic imaging , Orthodontic Appliance Design , Patient Compliance , Radiography, PanoramicABSTRACT
A specific double-antibody radioimmunoassay with a sensitivity of 2.5 ng/ml has been developed for measuring thyroglobulin (Tg) in human serum. As endogenous anti-Tg antibodies in serum interfere in the assay, only sera with a negative tanned red cell (TRC) test are suitable for analysis. Tg was detectable in 84.7% of the euthyroid subjects, with a mean value of 6.1 (values ranging from nondetectable to 43.0 ng/ml). Values were significantly higher in women than in men. Tg release by the thyroid appears to be under pituitary control, as suggested by TSH stimulation and T3 suppression tests. Elevated Tg levels were found in hyperthyroidism, simple goitre, and differentiated thyroid carcinoma. The significance of circulating Tg and the possible application of the Tg RIA are discussed.
Subject(s)
Thyroglobulin/blood , Adolescent , Adult , Aged , Child , Female , Goiter/blood , Humans , Hyperthyroidism/blood , Hypothyroidism/blood , Male , Middle Aged , Radioimmunoassay/methods , Thyroid Neoplasms/bloodABSTRACT
Simplification of radioimmunoassay procedures of urinary aldosterone-18-glucuronide was attempted, taking into consideration the aspects implied by the hydrolysis of urine and the assay itself. The procedure standardized for the hydrolysis step (samples diluted with a two-fold volume of 0.2 N HCl and incubated at 30 degrees C for 16-24 h) proved suitable in terms of practicability and accuracy. Aldosterone antisera, raised in the rabbit against an aldosterone-3-bovine albumin conjugate, were selected according to their specificity towards competing steroids. Depending on the characteristics of the antisera used, an assay of extracts, or even direct measurements of hydrolyzed urines excluding any extraction, were found to yield reliable results. In the case of a high-quality antiserum, evidence for the adequacy of assay on non-hydrolyzed urine extracts for the measurement of the excretion of unconjugated aldosterone was provided by some preliminary data. The results of the experiments, directed at the methodological and clinical validation of the simplified procedures, are reported and discussed in this paper.