Dual-recognition "turn-off-on" fluorescent Biosensor triphenylamine-based continuous detection of copper ion and glyphosate applicated in environment and living system.

Heavy metal Cu2+ emitted in industry and residues of glyphosate pesticides are pervasive in ecosystems, accumulated in water bodies and organisms' overtime, constituting hazard to human and ecological balance. The development of rapid, highly selective, reversibility and sensitive biosensor in vivo detection for Cu2+ and glyphosate was imminent. A novel dual-recognition fluorescence biosensor MPH was successfully synthesized based on triphenylamine, which demonstrated remarkable ratiometric fluorescence quenching toward Cu2+, while MPH-Cu2+ (1:1) ensemble exhibited ratiometric fluorescence restoration for glyphosate, both with observable color changes in daylight and UV lamp. The biosensor exhibited rapid, outstanding selectivity, anti-interference, and multiple cycles reversibility through "turn-off-on" fluorescence towards Cu2+ and glyphosate, respectively. Surprisingly, the clearly binding mechanisms of MPH to Cu2+ and MPH-Cu2+ ensemble to glyphosate were determined, respectively, based on the Job's plot, FT-IR, ESI-HRMS, 1H NMR titration and theoretical calculations of dynamics and thermodynamics. In addition, biosensor MPH demonstrated successful detection of Cu2+ and glyphosate across diverse environmental samples including tap water, extraction solutions of traditional Chinese medicine honeysuckle and soil samples. In the meantime, fluorescence imaging of Cu2+ and glyphosate at both micro and macro scales in various living organisms, such as rice roots, MCF-7 cells, zebrafish, and mice, were successfully achieved. Overall, this work was expected to become a promising and versatile fluorescence biosensor for rapid and reversible detection of Cu2+ and glyphosate both in vitro and vivo.

[Identifying Driving Factors and Their Interacting Effects on Sources of Heavy Metal in Farmland Soils with Geodetector and Multi-source Data].

The identification of heavy metal sources in farmland soils is essential for the rational health condition management and sustainable development of soil. Using source resolution results(source component spectrum and source contribution)of a positive matrix factorization(PMF)model, historical survey data, and time-series remote sensing data, integrating a geodetector(GD), an optimal parameters-based geographical detector(OPGD), a spatial association detector(SPADE), and an interactive detector for spatial associations(IDSA)model, this study explored the modifiable areal unit problem(MAUP) of spatial heterogeneity of soil heavy metal sources and identified the driving factors and their interacting effects on the spatial heterogeneity of soil heavy metal sources in categorical and continuous variables, respectively. The results showed that the spatial heterogeneity of soil heavy metal sources at small and medium scales was affected by the spatial scale, and the optional spatial unit was 0.08 km2 for detecting spatial heterogeneity of soil heavy metal sources in the study region. Considering spatial correlation and discretization level, the combination of the quantile method and discretization parameters with an interruption number of 10 could be implied to reduce the partitioning effects on continuous variables in the detection of spatial heterogeneity of soil heavy metal sources. Within categorical variables, strata(PD 0.12-0.48) controlled the spatial heterogeneity of soil heavy metal sources, the interaction between strata and watersheds explained 27.28%-60.61% of each source, and the high-risk areas of each source were distributed in the lower sinian system, upper cretaceous in strata, mining land in land use, and haplic acrisols in soil types. Within continuous variables, population (PSD 0.40-0.82) controlled the spatial variation in soil heavy metal sources, and the explanatory power of spatial combinations of continuous variables for each source ranged from 61.77% to 78.46%. The high-risk areas of each source were distributed in evapotranspiration (41.2-43 kg·m-2), distance from the river (315-398 m), enhanced vegetation index (0.796-0.995), and distance from the river (499-605 m). The results of this study provide a reference for the research of the drivers of heavy metal sources and their interactions in arable soils and provide an important scientific basis for the management of arable soil and its sustainable development in karst areas.