The mosquitoes of Finland: updated distributions and bionomics.

Mosquitoes (Diptera: Culicidae) were collected in Finland between 2012 and 2018 to determine the species present and their distributions. In total, 52 466 specimens from 1031 collections formed the basis for the preparation of distribution maps for each of the 40 species that were collected. Anopheles maculipennis s.s., An. claviger, Aedes geminus and Ochlerotatus sticticus are confirmed on mainland Finland after previous records were uncertain or absent. Coquillettidia richiardii, Culiseta morsitans, Cs. ochroptera, Culex territans, Cx. torrentium, Oc. leucomelas, Oc. nigrinus, Oc. pullatus and Oc. punctodes occur more widely than previously reported. Three species, Ae. rossicus, Cs. subochrea and Oc. cyprius were not collected, although Ae. rossicus was subsequently found in Lapland by another researcher. No invasive species were collected. Ochlerotatus communis, an aggressive biter, was the most commonly encountered species. Larval collection data suggest that several species may have up to three generations per year in Finland, with Cx. torrentium and Cx. pipiens having at least two, and Oc. communis and Oc. punctor regularly found as larvae across the summer. These data, especially when coupled with historical records, are vital for monitoring species which have significant vector potential, particularly when faced with a warming climate.

Anopheles daciae, a new country record for Finland.

Mosquitoes (Diptera: Culicidae) were collected throughout Finland between 2013 and 2018 to determine species distributions. During the course of molecular identifications of specimens belonging to the Anopheles maculipennis complex, ribosomal internal transcribed spacer 2 sequences and link-reared specimens revealed the presence of Anopheles daciae Linton, Nicolescu & Harbach (n = 37), a new country record, as well as Anopheles messeae Falleroni (n = 19) in the collections. Although the sample size is low, distinctions are apparent in the distributions of these two species, with An. daciae present in south-eastern and central Finland, including the regions of Kanta-Häme, Pirkanmaa, Pohjois-Pohjanmaa, Päijät-Häme and Satakunta, and An. messeae present in the southern and south-eastern regions of Åland (Ahvenanmaa), Etelä-Savo, Kanta-Häme, Kymenlaakso, Päijät-Häme, Satakunta, Uusimaa and Varsinais-Suomi. All reports of An. messeae in Finland prior to 2018 should therefore be recognized as potentially being either An. messeae or An. daciae. Because these species are potential vectors of malarial protozoa, it is important to have full knowledge of their distributions across Europe, particularly in the face of climate warming.

A report on the mosquitoes of mainland Åland, southwestern Finland and revised list of Finnish mosquitoes.

To successfully implement surveillance or control strategies for mosquitoes, up-to-date knowledge of regional species composition is vital. The last report regarding mosquitoes (Diptera: Culicidae) in the Åland archipelago, southwestern Finland listed 19 species (Utrio, 1979). To determine the current species diversity, one collection trip was made to mainland Åland in 2015 and three in 2016. Mosquitoes (n = 3286) were collected as both adult and immature life stages from 88 collections within 29 1-km2 areas. Fifteen of the 19 previously reported species were obtained, leaving the current status of four species uncertain. At least 11 species previously not reported from Åland, but confirmed on the Finnish mainland, were collected. Aedes geminus Peus was identified based on examination of the gonostylus, and represents a new species distribution for Finland. Anopheles maculipennis s.s. Meigen was confirmed from cytochrome c oxidase subunit I (COI) and internal transcribed spacer 2 (ITS2) sequence data and is reinstated on the list of Finnish species, along with Ochlerotatus sticticus (Meigen). Dahliana geniculata (Olivier) was found in two locations, in 2 months, indicating that there is an established population in Åland. The present data confirm that at least 27 species inhabit mainland Åland, rising to 31 when historical data are included. The Finnish mosquito fauna is increased from 38 to 41 species.

Why barcode? High-throughput multiplex sequencing of mitochondrial genomes for molecular systematics.

Mitochondrial genome sequences are important markers for phylogenetics but taxon sampling remains sporadic because of the great effort and cost required to acquire full-length sequences. Here, we demonstrate a simple, cost-effective way to sequence the full complement of protein coding mitochondrial genes from pooled samples using the 454/Roche platform. Multiplexing was achieved without the need for expensive indexing tags ('barcodes'). The method was trialled with a set of long-range polymerase chain reaction (PCR) fragments from 30 species of Coleoptera (beetles) sequenced in a 1/16th sector of a sequencing plate. Long contigs were produced from the pooled sequences with sequencing depths ranging from ∼10 to 100× per contig. Species identity of individual contigs was established via three 'bait' sequences matching disparate parts of the mitochondrial genome obtained by conventional PCR and Sanger sequencing. This proved that assembly of contigs from the sequencing pool was correct. Our study produced sequences for 21 nearly complete and seven partial sets of protein coding mitochondrial genes. Combined with existing sequences for 25 taxa, an improved estimate of basal relationships in Coleoptera was obtained. The procedure could be employed routinely for mitochondrial genome sequencing at the species level, to provide improved species 'barcodes' that currently use the cox1 gene only.