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Nucleosides Nucleotides Nucleic Acids ; 20(4-7): 1049-51, 2001.
Article in English | MEDLINE | ID: mdl-11562956

ABSTRACT

The synthesis of a fluorescein conjugate on the non-natural base P is described. The ability of the newly synthesised fluorescein--dPTP and of a fluorescein-11-dUTP to compete with the natural nucleotide TTP was also studied. Overall the efficiency of labelling a nucleic acid with a fluorescein moiety was found to be approximately equal.


Subject(s)
Fluoresceins/chemistry , Nucleotides/chemistry , Nucleotides/chemical synthesis , Oxazines/chemistry , Pyrenes/chemical synthesis , Pyrimidines/chemistry , Binding, Competitive , DNA Polymerase I/metabolism , Deoxyuracil Nucleotides/metabolism , Fluoresceins/chemical synthesis , Fluoresceins/metabolism , Nucleotides/metabolism , Oxazines/metabolism , Pyrimidines/metabolism
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