ABSTRACT
BACKGROUND: The study was conducted to assess the effects of the monophasic combined oral contraceptive containing ethinyl estradiol (EE) 0.03 mg and chlormadinone acetate (CMA) 2 mg (EE/CMA) on papulopustular acne of the face, décolleté (low neck) and back; on moderate comedonal acne of the face; and on seborrhea, alopecia and hirsutism. STUDY DESIGN: Three hundred seventy-seven women were randomized (2:1) to receive EE/CMA (n=251) or placebo (n=126) for six medication cycles. Due to the placebo-controlled, double-blind design of the trial, condoms were supplied for contraception. The primary efficacy end point was defined as a reduction of at least 50% in the number of papules and/or pustules of the face from admission to Medication Cycle 6. RESULTS: In total, 64.1% (161/251) of subjects treated with EE/CMA responded compared with 43.7% (55/126) of those taking placebo (p=.0001). The median reduction in papules/pustules on the face at Cycle 6 compared with admission was 63.6% (EE/CMA) compared with 45.3% (placebo group). For comedonal lesions of the face, the reduction in lesion numbers was 54.8% (EE/CMA) compared with 32.4% (placebo). Moderate papulopustular acne of the décolleté decreased by 92.9% (EE/CMA) vs. 50% (placebo group) and of the back by 86.0% and 58.3%, respectively. For these skin conditions, the p values for the relative difference between groups vs. baseline were <.05 at Cycles 3 and 6, in favor of EE/CMA. As part of a self-assessment rating, at least 70.5% (EE/CMA) vs. 41.3% (placebo) reported an at least satisfactory improvement of their moderate acne. Even 39.8% of women taking EE/CMA reported an "excellent improvement" or "complete resolution" of moderate acne compared with 12.7% taking placebo. CONCLUSION: In addition to its contraceptive efficacy described elsewhere, EE/CMA is an effective treatment for moderate papulopustular acne and other androgen-related skin disorders.
Subject(s)
Acne Vulgaris/drug therapy , Androgen Antagonists/therapeutic use , Chlormadinone Acetate/therapeutic use , Estrogens/therapeutic use , Ethinyl Estradiol/therapeutic use , Adolescent , Adult , Alopecia/drug therapy , Androgen Antagonists/adverse effects , Chlormadinone Acetate/adverse effects , Dermatitis, Seborrheic/drug therapy , Double-Blind Method , Drug Therapy, Combination , Estrogens/adverse effects , Ethinyl Estradiol/adverse effects , Female , Hirsutism/drug therapy , Humans , Sex Hormone-Binding Globulin/metabolism , Young AdultABSTRACT
PURPOSE: Scoring systems to predict mortality from surgery are important tools used to give information to the operator and patient and in the auditing of clinical practice. This study was designed to validate the recently developed the Colorectal Physiologic and Operative Severity Score for the Enumeration of Mortality and Morbidity (CR-POSSUM) scoring system in a single center for colorectal cancer surgery. We also analyzed whether albumin may have a role in the CR-POSSUM model. METHODS: We compared this model with two other scoring systems: POSSUM and Portsmouth-POSSUM (P-POSSUM) models. In-hospital mortality was used as the outcome, and Hosmer-Lemeshow statistic was used to determine goodness of fit. RESULTS: Complete data were collected prospectively from 304 patients from 1990 to the present. The overall operative mortality was 6.5 percent. Observed to expected ratios were used to compare the scoring systems at a given predicted mortality. The overall observed to expected ratio was 1.25 for CR-POSSUM, 1.59 for P-POSSUM, and 3.37 for POSSUM. The CR-POSSUM model showed a good fit with the data (Hosmer-Lemeshow statistic, 3.86; P = 0.795) and the area under the receiver operator curve was 0.74. After correcting for factors used in the CR-POSSUM, logistic regression showed a significant correlation between albumin and mortality (P = 0.016). CONCLUSIONS: We have shown that the CR-POSSUM model is an accurate predictor of outcome for major colorectal surgery. The POSSUM and P-POSSUM models over-predicted mortality. Albumin, which is not a factor included in these three systems, may be an important addition in improving the accuracy of the CR-POSSUM model.
Subject(s)
Colorectal Neoplasms/mortality , Colorectal Neoplasms/surgery , Risk Assessment/methods , Adult , Aged , Aged, 80 and over , Female , Humans , Logistic Models , Male , Middle Aged , Predictive Value of Tests , Prospective Studies , ROC Curve , Risk Factors , Severity of Illness IndexABSTRACT
OBJECTIVE: To establish the efficacy and safety of silicic acid in comparison to placebo in patients with mild to moderate acne vulgaris. PATIENTS AND METHODS: Forty adult patients with facial acne vulgaris were randomized to silicic acid or placebo applied twice daily for 8 weeks. Efficacy was assessed by number of acne lesions, acne grade and sebum excretion rate (SER). Clinical safety and tolerance were also assessed and global assessments and psychological profile questionnaires were completed. RESULTS: There were no significant differences between silicic acid and placebo in the number of inflamed lesions (the primary efficacy variable). In contrast, silicic acid significantly reduced the number of non-inflamed lesions, total acne lesions and the acne grade after 8 weeks and SER over 4 weeks when compared with placebo. There were no significant differences between silicic acid and placebo in the responses to the questionnaires or the global assessments after 8 weeks. Silicic acid was well tolerated with few adverse events. CONCLUSIONS: Silicic acid may have a positive treatment effect on the non-inflammatory component of mild to moderate facial acne. These preliminary findings now need to be confirmed by similar studies.
Subject(s)
Acne Vulgaris/drug therapy , Silicic Acid/therapeutic use , Acne Vulgaris/pathology , Adolescent , Adult , Double-Blind Method , Female , Humans , Male , Prospective Studies , Treatment OutcomeABSTRACT
BACKGROUND: We investigated the efficacy and cost-effectiveness of five antimicrobial regimens for mild to moderate facial acne and whether propionibacterial antibiotic resistance affects treatment response. METHODS: In this randomised, observer-masked trial, 649 community participants were allocated one of five antibacterial regimens. Primary outcomes were patients' self-assessed improvement and reduction in inflamed lesions at 18 weeks. Analyses were by intention to treat. FINDINGS: Moderate or greater improvement at 18 weeks was reported in 72 (55%) of 131 participants assigned oral oxytetracycline plus topical placebo, 70 (54%) of 130 assigned oral minocycline plus topical placebo, 78 (60%) of 130 assigned topical benzoyl peroxide plus oral placebo, 84 (66%) of 127 assigned topical erythromycin and benzoyl peroxide in a combined formulation plus oral placebo, and 82 (63%) of 131 assigned topical erythromycin and benzoyl peroxide separately plus oral placebo. Most improvement occurred in the first 6 weeks. Treatment differences for the proportion of people with at least moderate improvement were: minocycline versus oxytetracycline -1.2% (unadjusted 95% CI -13.3 to 10.9); combined erythromycin and benzoyl peroxide versus oxytetracycline 11.1% (-0.7 to 22.9) and versus minocycline 12.3% (0.4 to 24.2); erythromycin and benzoyl peroxide separately versus combined formulation -3.5% (-15.2 to 8.2); benzoyl peroxide versus oxytetracycline 5.0% (-7.0 to 17.0), versus minocycline 6.2% (-5.8 to 18.2), and versus combined formulation -6.1% (-17.9 to 5.7). Benzoyl peroxide was the most cost-effective treatment. Efficacy of both tetracyclines was reduced by pre-existing tetracycline resistance. INTERPRETATION: Topical benzoyl peroxide and benzoyl peroxide/erythromycin combinations are similar in efficacy to oral oxytetracycline and minocycline and are not affected by propionibacterial antibiotic resistance.
Subject(s)
Acne Vulgaris/drug therapy , Anti-Bacterial Agents/administration & dosage , Facial Dermatoses/drug therapy , Acne Vulgaris/economics , Acne Vulgaris/microbiology , Administration, Oral , Administration, Topical , Adolescent , Adult , Anti-Bacterial Agents/adverse effects , Anti-Bacterial Agents/economics , Benzoyl Peroxide/administration & dosage , Child , Cost-Benefit Analysis , Erythromycin/administration & dosage , Erythromycin/adverse effects , Erythromycin/economics , Facial Dermatoses/microbiology , Female , Humans , Male , Minocycline/administration & dosage , Minocycline/adverse effects , Minocycline/economics , Oxytetracycline/administration & dosage , Oxytetracycline/adverse effects , Oxytetracycline/economics , Single-Blind Method , Skin/microbiologyABSTRACT
An important feature in the etiology of acne is the presence of pilosebaceous ductal hypercornification, which can be seen histologically as microcomedones (Fig 1) and clinically as blackheads, whiteheads, and other forms of comedones, such as macrocomedones. There is a significant correlation between the severity of acne and the number and size of microcomedones (follicular casts), the presence of which is a measure of comedogenesis. This correlation can be demonstrated by skin surface biopsy using cyanoacrylate gel. In this procedure, microcomedones are sampled by applying cyanoacrylate gel to the skin surface. A glass microscopic slide is then applied on top of the gel and pressed firmly onto the skin for 1 minute(1-3). The glass slide is gently removed, taking with it the upper part of the stratum corneum and microcomedones, which are then analyzed by low-power microscopy or digital image analysis.(1-3)
Subject(s)
Acne Vulgaris/etiology , Acne Vulgaris/pathology , Acne Vulgaris/therapy , Biopsy/methods , Epidermis/pathology , Hair Follicle/pathology , Humans , Image Processing, Computer-Assisted/methods , Sebaceous Glands/pathologyABSTRACT
Acne, a chronic inflammatory disease of the pilosebaceous units of the face, neck, chest, and back, is the most common skin disorder occurring universally, with an estimated prevalence of 70-87%.(1) It is a pleomorphic disorder characterized by both inflammatory (papules, pustules, nodules) and noninflammatory (comedones, open and closed) lesions. Grading of acne is mandatory to determine the appropriate therapeutic strategy. Mild acne can be purely comedonal or mild papulopustular, with a few papulopustules present as well.(2) Moderate acne is characterized by numerous comedones, few to many pustules, and few small nodules, with no residual scarring.(2) In severe acne papulopustules are numerous, many nodules can be detected, inflammation is marked, and scarring is present.(2) Very severe acne can be recognized by sinus tracts, grouped comedones, many deeply located nodules, and severe inflammation and scarring.(2) Although acne does not affect health overall, its impact on emotional well-being and function can be critical and is often associated with depression, anxiety, and higher-than-average unemployment rates.(3) Effective treatment can dramatically improve a person's quality of life.
Subject(s)
Acne Vulgaris/drug therapy , Clinical Protocols , Dermatologic Agents/therapeutic use , Acne Vulgaris/classification , Acne Vulgaris/psychology , Administration, Cutaneous , Administration, Oral , Anti-Bacterial Agents/therapeutic use , Humans , Quality of Life , Retinoids/therapeutic useABSTRACT
This multicenter, randomized, investigator-blinded study compared the efficacy and tolerability of a combination of lymecycline 300 mg/day orally and adapalene topical gel 0.1% (n = 118) to lymecycline 300 mg/day orally plus vehicle gel (n = 124) in patients with moderate to moderately severe acne vulgaris with both inflammatory and noninflammatory lesions. The primary efficacy end point, total lesion count at end point (last observation carried forward), showed a statistically significant difference in favor of the lymecycline plus adapalene group (P =.0011). The mean decrease in total, inflammatory and noninflammatory lesion counts was significantly greater at end point in the lymecycline plus adapalene group than in the lymecycline plus vehicle group (P <.01). In addition, a significant difference for inflammatory and total acne lesions was seen sooner in the adapalene plus lymecycline group. In total, 75.5% of patients in the lymecycline plus adapalene group were markedly improved, almost clear or clear of their lesions at week 12, compared with 51.8% of those in the lymecycline plus vehicle group (P <.001). Local cutaneous tolerance was generally good in both groups, although more patients receiving the lymecycline plus adapalene combination experienced cutaneous reactions than those receiving lymecycline plus vehicle. There are relatively few studies comparing the efficacy of combined oral and topical therapy with either individual therapy alone. This study clearly demonstrates that lymecycline plus adapalene combination treatment resulted in a significantly greater mean decrease in the number of inflammatory, noninflammatory and total lesions than lymecycline plus vehicle and was well tolerated.
Subject(s)
Acne Vulgaris/drug therapy , Lymecycline/therapeutic use , Naphthalenes/therapeutic use , Acne Vulgaris/diagnosis , Adapalene , Administration, Oral , Administration, Topical , Adolescent , Adult , Child , Dose-Response Relationship, Drug , Drug Administration Schedule , Drug Therapy, Combination , Female , Follow-Up Studies , Humans , Male , Probability , Reference Values , Severity of Illness Index , Single-Blind Method , Treatment OutcomeABSTRACT
The earliest subclinical acne "lesion" is a microcomedone, of which hyperproliferation of the follicular epithelium is a characteristic feature. Inflammatory cells have been observed at the periphery of these "lesions". This study investigated whether inflammatory events occur pre or post hyperproliferative changes. Cellular, vascular, and proliferative markers were examined by immunohistochemical techniques on biopsies of clinically normal follicles from uninvolved skin and early inflamed lesions from acne patients. Control follicles were obtained from non-acne subjects. Follicles from uninvolved skin exhibited no microcomedonal features. Proliferation in the epithelium was comparable to controls and was significantly lower than in inflamed lesions. Numbers of CD3+, CD4+ T cells were elevated in the perifollicular and papillary dermis although levels were not equivalent to those in papules. The number of macrophages was also greatly increased and similar to those in papules. There were no changes in blood vessel numbers or vascular intercellular adhesion molecule 1 expression but E-selectin expression was increased to levels found in papules and vascular adhesion molecule 1 levels were upregulated. Levels of the pro-inflammatory cytokine interleukin-1 were also upregulated perifollicularly. Moreover, aberrant integrin expression was demonstrated in the epidermis around these uninvolved follicles and inflamed lesions whereas the basement membrane was still intact. These results provide novel evidence for vascular endothelial cell activation and involvement of inflammatory responses in the very earliest stages of acne lesion development.
Subject(s)
Acne Vulgaris/immunology , Acne Vulgaris/pathology , Hair Follicle/immunology , Hair Follicle/pathology , Adolescent , Adult , Antigens, CD/analysis , Antigens, CD1/analysis , Antigens, Differentiation, Myelomonocytic/analysis , Biomarkers , Blood Vessels/chemistry , Blood Vessels/cytology , CD3 Complex/analysis , CD4-Positive T-Lymphocytes/chemistry , CD4-Positive T-Lymphocytes/cytology , CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/chemistry , CD8-Positive T-Lymphocytes/cytology , CD8-Positive T-Lymphocytes/immunology , Cell Differentiation/immunology , Cell Division/immunology , Collagen Type IV/analysis , HLA-DR Antigens/analysis , Hair Follicle/chemistry , Humans , Immunohistochemistry , Immunologic Memory , Integrin alpha6/analysis , Interleukin-1/analysis , Keratinocytes/immunology , Keratinocytes/pathology , Laminin/analysis , Langerhans Cells/chemistry , Langerhans Cells/cytology , Langerhans Cells/immunology , Leukocyte Common Antigens/analysis , Macrophages/chemistry , Macrophages/cytology , Macrophages/immunology , Neutrophils/cytology , Neutrophils/immunology , Receptors, Interleukin-1/analysis , Receptors, Interleukin-1 Type IISubject(s)
Acne Vulgaris/drug therapy , Hydroxyurea/analogs & derivatives , Hydroxyurea/therapeutic use , Lipoxygenase Inhibitors/therapeutic use , Acne Vulgaris/pathology , Administration, Oral , Adolescent , Female , Humans , Hydroxyurea/administration & dosage , Lipoxygenase Inhibitors/administration & dosage , Male , Pilot ProjectsABSTRACT
BACKGROUND: One approach to suppressing the overgrowth of antibiotic-resistant bacteria is to develop combination products composed of active constituents with complementary but distinct mechanisms of antibacterial action. OBJECTIVE: The purpose of this study was to compare the antimicrobial and clinical efficacy and tolerability of clindamycin phosphate 1%/benzoyl peroxide 5% gel formulation with matching clindamycin 1% gel in the treatment of acne vulgaris. METHODS: This 16-week, single-center, double-blind, randomized, parallel-group study compared the combination gel with clindamycin monotherapy applied BID in patients 13 to 30 years of age with mild to moderate acne and facial Propionibacterium acnes counts > or = 10(4) colony-forming units per square centimeter of skin. RESULTS: Seventy-nine patients were enrolled and randomly assigned to receive the combination gel (n = 40) or clindamycin monotherapy (n = 39). Seventy patients (50 males, 20 females; mean age, 18.2 years) were included in the intent-to-treat group. The combination gel treatment produced significantly greater reductions (P < or = 0.046) from baseline in total lesion counts and in numbers of inflammatory lesions and comedones compared with clindamycin monotherapy. Greater reductions in the severity of acne also were observed in the physician's and patient's Clinical Global Improvement scale scores and in other secondary efficacy measurements. Reductions in clindamycin-resistant P acnes counts were observed relative to baseline in the combination gel group; in contrast, P acnes counts increased by >1,600% in the clindamycin monotherapy group at week 16 (P = 0.018 vs combination gel). Reductions in inflammatory (r2 = 0.31; P = 0.016) and total (r2 = 0.28; P = 0.027) lesions were correlated with decreases in clindamycin-resistant bacteria. Also, significant correlations were observed between the percent change from baseline in total lesion counts (r2 = 0.44; P < 0.001) and comedo counts (r2 = 0.50; P < 0.001) and the log10 change from baseline in total P acnes counts. CONCLUSIONS: The total P acnes count (P = 0.002) and the clindamycin-resistant P acnes count (P = 0.018) were significantly reduced after 16 weeks of treatment with combination gel compared with clindamycin monotherapy. These reductions in total P acnes and clindamycin-resistant P acnes counts correlated with reductions in total acne lesions.
Subject(s)
Acne Vulgaris/drug therapy , Anti-Bacterial Agents/administration & dosage , Benzoyl Peroxide/administration & dosage , Clindamycin/administration & dosage , Gram-Positive Bacterial Infections/drug therapy , Acne Vulgaris/microbiology , Administration, Topical , Adolescent , Anti-Bacterial Agents/therapeutic use , Benzoyl Peroxide/therapeutic use , Clindamycin/therapeutic use , Colony Count, Microbial , Double-Blind Method , Drug Combinations , Drug Resistance, Bacterial , Female , Gram-Positive Bacterial Infections/microbiology , Humans , Male , Propionibacterium acnes/drug effects , Propionibacterium acnes/isolation & purification , Treatment OutcomeABSTRACT
BACKGROUND: Previous clinical trials have shown that adapalene gel produces less irritation than tretinoin gels and tretinoin 0.025% cream. Short term results have shown that adapalene is less irritating than tretinoin gels and creams. This study is the first to compare the 0.1% formulation of adapalene gel with the 0.05% strength of tretinoin cream in a formal clinical trial. OBJECTIVE: To investigate the efficacy and tolerability of adapalene gel 0.1% compared with tretinoin cream 0.05% in patients with mild-to-moderate acne vulgaris. METHODS: Ten-week, multicentre, randomised, investigator-masked, active-controlled, parallel group study in 409 patients with acne vulgaris. RESULTS: Adapalene gel 0.1% demonstrated equivalent efficacy in reduction of acne lesion counts and global improvement of acne severity over 10 weeks' treatment and was significantly better tolerated than tretinoin cream 0.05% in terms of erythema, dryness, desquamation and stinging/burning. CONCLUSION: Adapalene gel 0.1% showed equivalent efficacy and was significantly better tolerated than tretinoin cream 0.05% in patients with mild-to-moderate acne vulgaris.
Subject(s)
Acne Vulgaris/drug therapy , Dermatologic Agents/therapeutic use , Keratolytic Agents/therapeutic use , Naphthalenes/therapeutic use , Tretinoin/therapeutic use , Adapalene , Administration, Cutaneous , Adolescent , Adult , Child , Dermatologic Agents/administration & dosage , Female , Humans , Keratolytic Agents/administration & dosage , Male , Naphthalenes/administration & dosage , Ointments , Treatment Outcome , Tretinoin/administration & dosageABSTRACT
One of the factors that contributes to the pathogenesis of acne is Propionibacterium acnes; yet, the molecular mechanism by which P. acnes induces inflammation is not known. Recent studies have demonstrated that microbial agents trigger cytokine responses via Toll-like receptors (TLRs). We investigated whether TLR2 mediates P. acnes-induced cytokine production in acne. Transfection of TLR2 into a nonresponsive cell line was sufficient for NF-kappa B activation in response to P. acnes. In addition, peritoneal macrophages from wild-type, TLR6 knockout, and TLR1 knockout mice, but not TLR2 knockout mice, produced IL-6 in response to P. acnes. P. acnes also induced activation of IL-12 p40 promoter activity via TLR2. Furthermore, P. acnes induced IL-12 and IL-8 protein production by primary human monocytes and this cytokine production was inhibited by anti-TLR2 blocking Ab. Finally, in acne lesions, TLR2 was expressed on the cell surface of macrophages surrounding pilosebaceous follicles. These data suggest that P. acnes triggers inflammatory cytokine responses in acne by activation of TLR2. As such, TLR2 may provide a novel target for treatment of this common skin disease.
Subject(s)
Acne Vulgaris/immunology , Cytokines/biosynthesis , Drosophila Proteins , Membrane Glycoproteins/physiology , Propionibacterium acnes/physiology , Receptors, Cell Surface/physiology , Acne Vulgaris/etiology , Animals , Cell Line , Humans , Interleukin-12/biosynthesis , Interleukin-12/genetics , Interleukin-18/biosynthesis , Macrophages/metabolism , Mice , Monocytes/metabolism , Promoter Regions, Genetic , Toll-Like Receptor 1 , Toll-Like Receptor 2 , Toll-Like ReceptorsABSTRACT
Five independent clones of the Propionibacterium acnes P-37 lipase gene (gehA) were obtained in Escherichia coli, and the gene was localized to a 2.75 kb Xhol fragment by subcloning. The five clones were shown to contain the same gene by Southern blotting with a DIG-labelled probe to gehA. The nucleotide sequence of gehA was determined, and shown to contain a single ORF of 1017 kb, encoding a protein of 339 amino acids. The predicted molecular mass was 36 kDa. A 33 kDa (PAGE) radiolabelled polypeptide was detected from E. coli minicell preparations harbouring gehA, which could correspond to GehA after cleavage of the putative 26 amino acid residue signal peptide. gehA was overexpressed in E. coli under the control of the bacteriophage T7 promoter, and the corresponding polypeptide was found to be present in insoluble aggregates. Active lipase was produced when the overexpressing strain was incubated at a reduced temperature in the presence of sucrose. Purification of lipase from P. acnes culture supernatant fluids confirmed the production of a 33 kDa (PAGE) lipase.