ABSTRACT
A polymerase chain reaction-based method was used to screen sandflies for infection with Wolbachia (Rickettsiales: Rickettsiaceae), an intracellular bacterial endosymbiont found in many arthropods and filarial hosts. Positive results were obtained in five of 200 field-collected sandflies and were confirmed by sequencing. All sandflies were Lutzomyia longipalpis (Diptera: Psychodidae) captured in a region endemic for visceral leishmaniasis in Brazil. This is the first study to identify Wolbachia infection in this Lutzomyia species, which is the main vector of leishmaniasis in the study area. The low infection rate found in this study (2.5%), together with the lack of detection of Wolbachia in previous studies and the diversity found in the sequences analysed, suggests horizontal transmission to these sandflies.
Subject(s)
Insect Vectors/microbiology , Psychodidae/microbiology , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Wolbachia/isolation & purification , Animals , Brazil , Communicable Disease Control/methods , Female , Haplotypes , Leishmania infantum/physiology , Leishmaniasis, Visceral/transmission , Male , Polymerase Chain Reaction , Sequence Analysis, RNAABSTRACT
Evaluar la asociación entre hábitos de succión no nutritivos y la práctica del amamantamiento materno. Material y Métodos: Fueron realizadas entrevistas con 87 embarazadas y visitas mensualmente hasta los 6 meses de edad de los bebes, abordando preguntas sobre condiciones socio económica-culturales, práctica de amamantamiento y hábitos. Resultados: Casi la totalidad (94,7%) de las madres empezó a amamantar a los bebes en el primer mes, pero apenas el 49,7% amamantaban exclusivamente del seno. Al fin del sexto mes ninguna madre estaba amamantando exclusivamente y un 50% de los niños habían sido destetados. Los hábitos y uso de chupete fueron asociados significantemente con niños amamantados bajo amamantamiento exclusivo (p=0,0065 y 0,0270 respectivamente) y las amamantadas en el seno (los dos p<0,0001). La tasa de destete precoz fue elevada y baja la prevalencia de amamantamiento materno exclusivo, demostrando asociación entre hábitos y uso de chupete con amamantamiento materno
Verifying the association with non-nutritives suction habits and practice of breastfeeding. Material and Methods: 87 pregnants were interviewed and were monthly home visits until 6 mounths old babies, the studied variables were: social-economic-culture conditions, breast feeding pratical and non-nutritives suction habits. Results: 94.7% for mothers started breastfeeding their babies in the first month, but only 49.7% exclusively children breastfeeding. The sixth month nobody mother non-exclusively breastfed ones and 50% were weaning. The habits and pacifier users were significant association with exclusively children breastfed (p=0.0065 and 0.0270 respectively) and children breastfed (both p<0.0001). The early weaning rate was high and low rate of exclusive breastfeeding, existing association between non-nutritives suction habits and pacifier users with breastfeeding